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Calculations in molecular biology and biotechnology [[electronic resource] ] : a guide to mathematics in the laboratory / / Frank H. Stephenson
| Calculations in molecular biology and biotechnology [[electronic resource] ] : a guide to mathematics in the laboratory / / Frank H. Stephenson |
| Autore | Stephenson Frank Harold |
| Pubbl/distr/stampa | Amsterdam ; ; Boston, : Academic Press, c2003 |
| Descrizione fisica | 1 online resource (319 p.) |
| Disciplina | 572.8/01/51 |
| Soggetto topico |
Molecular biology - Computer simulation
Molecular biology - Mathematics Biotechnology |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-02727-8
9786611027278 0-08-050061-7 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Calculations in Molecular Biology and Biotechnology; Copyright Page; Contents; Foreword; Chapter 1. Scientific Notation and Metric Prefixes; Introduction; Significant Digits; Rounding Off Significant Digits in Calculations; Exponents and Scientific Notation; Expressing Numbers in Scientific Notation; Converting Numbers from Scientific Notation to Decimal Notation; Adding and Subtracting Numbers Written in Scientific Notation; Multiplying and Dividing Numbers Written in Scientific Notation; Metric Prefixes; Conversion Factors and Canceling Terms
Chapter 2. Solutions Mixtures and Media Introduction; Calculating Dilutions: A General Approach; Concentrations by a Factor of X; Preparing Percent Solutions; Diluting Percent Solutions; Moles and Molecular Weight: Definitions; Molarity; Diluting Molar Solutions; Converting Molarity to Percent; Converting Percent to Molarity; Normality; PH; pKa and the Henderson-Hasselbalch Equation; Chapter 3. Cell Growth; The Bacterial Growth Curve; Manipulating Cell Concentration; Plotting OD550 vs. Time on a Linear Graph; Plotting the Logarithm of OD550 vs. Time on a Linear Graph Plotting the Log of Cell Concentration vs. Time Calculating Generation Time; Plotting Cell Growth Data on a Semilog Graph; Determining Generation Time Directly from a Semilog Plot of Cell Concentration vs. Time; Plotting Cell Density versus OD550 on a Semilog Graph; The Fluctuation Test; Fluctuation Test Example; Variance; Measuring Mutation Rate; Measuring Cell Concentration on a Hemocytometer; Chapter 4. Working with Bacteriophage; Introduction; Multiplicity of Infection; Probabilities and Multiplicity of Infection; Measuring Phage Titer; Diluting Bacteriophage; Measuring Burst Size Chapter 5. Quantitation of Nucleic Acids Quantitation of Nucleic Acids by Ultraviolet Spectroscopy; Determining the Concentration of Double-Stranded DNA; Using Absorbance and an Extinction Coefficient to Calculate Double-Stranded DNA Concentration; Calculating DNA Concentration as a Millimolar (mM) Amount; Determining the Concentration of Single-Stranded DNA Molecules; Oligonucleotide Quantitation; Measuring RNA Concentration; Molecular Weight, Molarity, and Nucleic Acid Length; Estimating DNA Concentration on an Ethidium Bromide-Stained Gel Chapter 6. Labeling Nucleic Acids with Radioisotopes Introduction; Using Radioactivity: The Curie; Estimating Plasmid Copy Number; Labeling DNA by Nick Translation; Random Primer Labeling of DNA; Labeling 3' Termini with Terminal Transferase; cDNA Synthesis; Homopolymeric Tailing; In Vitro Transcription; Chapter 7. Oligonucleotide Synthesis; Introduction; Synthesis Yield; Measuring Stepwise and Overall Yield by the DMT Cation Assay; Overall Yield; Stepwise Yield; Calculating Micromoles of Nucleoside Added at Each Base Addition Step; Chapter 8. The Polymerase Chain Reaction; Introduction Template and Amplification |
| Record Nr. | UNINA-9910458136503321 |
Stephenson Frank Harold
|
||
| Amsterdam ; ; Boston, : Academic Press, c2003 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Calculations in molecular biology and biotechnology [[electronic resource] ] : a guide to mathematics in the laboratory / / Frank H. Stephenson
| Calculations in molecular biology and biotechnology [[electronic resource] ] : a guide to mathematics in the laboratory / / Frank H. Stephenson |
| Autore | Stephenson Frank Harold |
| Pubbl/distr/stampa | Amsterdam ; ; Boston, : Academic Press, c2003 |
| Descrizione fisica | 1 online resource (319 p.) |
| Disciplina | 572.8/01/51 |
| Soggetto topico |
Molecular biology - Computer simulation
Molecular biology - Mathematics Biotechnology |
| ISBN |
1-281-02727-8
9786611027278 0-08-050061-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Calculations in Molecular Biology and Biotechnology; Copyright Page; Contents; Foreword; Chapter 1. Scientific Notation and Metric Prefixes; Introduction; Significant Digits; Rounding Off Significant Digits in Calculations; Exponents and Scientific Notation; Expressing Numbers in Scientific Notation; Converting Numbers from Scientific Notation to Decimal Notation; Adding and Subtracting Numbers Written in Scientific Notation; Multiplying and Dividing Numbers Written in Scientific Notation; Metric Prefixes; Conversion Factors and Canceling Terms
Chapter 2. Solutions Mixtures and Media Introduction; Calculating Dilutions: A General Approach; Concentrations by a Factor of X; Preparing Percent Solutions; Diluting Percent Solutions; Moles and Molecular Weight: Definitions; Molarity; Diluting Molar Solutions; Converting Molarity to Percent; Converting Percent to Molarity; Normality; PH; pKa and the Henderson-Hasselbalch Equation; Chapter 3. Cell Growth; The Bacterial Growth Curve; Manipulating Cell Concentration; Plotting OD550 vs. Time on a Linear Graph; Plotting the Logarithm of OD550 vs. Time on a Linear Graph Plotting the Log of Cell Concentration vs. Time Calculating Generation Time; Plotting Cell Growth Data on a Semilog Graph; Determining Generation Time Directly from a Semilog Plot of Cell Concentration vs. Time; Plotting Cell Density versus OD550 on a Semilog Graph; The Fluctuation Test; Fluctuation Test Example; Variance; Measuring Mutation Rate; Measuring Cell Concentration on a Hemocytometer; Chapter 4. Working with Bacteriophage; Introduction; Multiplicity of Infection; Probabilities and Multiplicity of Infection; Measuring Phage Titer; Diluting Bacteriophage; Measuring Burst Size Chapter 5. Quantitation of Nucleic Acids Quantitation of Nucleic Acids by Ultraviolet Spectroscopy; Determining the Concentration of Double-Stranded DNA; Using Absorbance and an Extinction Coefficient to Calculate Double-Stranded DNA Concentration; Calculating DNA Concentration as a Millimolar (mM) Amount; Determining the Concentration of Single-Stranded DNA Molecules; Oligonucleotide Quantitation; Measuring RNA Concentration; Molecular Weight, Molarity, and Nucleic Acid Length; Estimating DNA Concentration on an Ethidium Bromide-Stained Gel Chapter 6. Labeling Nucleic Acids with Radioisotopes Introduction; Using Radioactivity: The Curie; Estimating Plasmid Copy Number; Labeling DNA by Nick Translation; Random Primer Labeling of DNA; Labeling 3' Termini with Terminal Transferase; cDNA Synthesis; Homopolymeric Tailing; In Vitro Transcription; Chapter 7. Oligonucleotide Synthesis; Introduction; Synthesis Yield; Measuring Stepwise and Overall Yield by the DMT Cation Assay; Overall Yield; Stepwise Yield; Calculating Micromoles of Nucleoside Added at Each Base Addition Step; Chapter 8. The Polymerase Chain Reaction; Introduction Template and Amplification |
| Record Nr. | UNINA-9910784562903321 |
|
Stephenson Frank Harold
|
||
| Amsterdam ; ; Boston, : Academic Press, c2003 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||