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Peptidomics [[electronic resource] ] : methods and applications / / edited by Mikhail Soloviev, Chris Shaw, Per Andrén
| Peptidomics [[electronic resource] ] : methods and applications / / edited by Mikhail Soloviev, Chris Shaw, Per Andrén |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Interscience, c2008 |
| Descrizione fisica | 1 online resource (432 p.) |
| Disciplina | 612/.015756 |
| Altri autori (Persone) |
SolovievMikhail <1965->
ShawChris <1954-> AndrénPer |
| Soggetto topico |
Peptides
Proteomics |
| ISBN |
1-281-22166-X
9786611221669 0-470-19650-5 0-470-19649-1 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
PEPTIDOMICS; CONTENTS; Preface; Contributors; PART I STUDIES OF NATURALLY OCCURRING PEPTIDES; 1 Analysis of the Peptidomes of Amphibian Skin Granular Gland Secretions-An Integrated Functional Genomic Strategy; 1.1 Introduction; 1.2 Historical Perspective; 1.3 Contemporary Methods of Sample Acquisition; 1.4 The Integrated Functional Genomic Strategy; 1.5 How then can All Three Approaches be Integrated?; 1.6 Limitations of Each Approach; 1.7 Closing Thoughts
2 A Short History of Insect (Neuro)Peptidomics-A Personal Story of the Birth and Youth of an Excellent Model For Studying Peptidome Biology2.1 Introduction; 2.2 History; 2.3 Present (and Future): Novel Technologies, New Data. . .; 2.3.1 MALDI and (Nano)ESI TOFs and QTOFs; 2.3.2 Peptide Displays; 2.3.3 Ion Traps and FT-ICRs: Greater Variety of Instruments, Greater Variety of Sequences. . .; 2.3.4 Orbitrap; 2.3.5 FT-ICRs; 2.3.6 Combining Peptide Chemical Analysis with Histological Localization: Peptidome Mass Spectrometry Imaging; 2.4 Concluding Remarks 3 Peptidomics of Short Linear Cytolytic Peptides from Spider Venom3.1 Introduction; 3.2 Peptidomics: Identifying and Sequencing Novel AMPs; 3.3 Genomics: Unraveling the Biosynthetic Pathway; 3.4 Venomics: AMPs in the Spider Venom; 3.4.1 Structural Features; 3.4.2 Functional Features; 3.4.3 Venomic Significance; 4 Molecular Cloning Approaches to Peptidomics: The Identification of Novel cDNAs Encoding Neurotoxin-like Peptide Pools; 4.1 Introduction; 4.2 Spider Toxins-Combinatorial Peptide Libraries; 4.3 EST Cloning Approach to Cloning Peptide Families 4.4 PCR-Based Amplification and Cloning Approaches4.5 The Design and Use of Hybrid Primers for the Amplification of cDNAs Coding for Large Toxin-like Peptide Pools; 4.5.1 Multiple Alignment of the Existing Toxins and Toxin-like Sequences; 4.5.2 The Design of Hybrid Partially Degenerate Primers; 4.5.2.1 The Design of the 3 ́ Region; 4.5.2.2 The Design of the 5 ́ Region and the Middle Part of the Primers; 4.5.2.3 The Overall Length and Degeneracy; 4.5.2.4 Annealing Temperature Matching; 4.5.3 Optimization of the Amplification Conditions; 4.5.4 Other Experimental Details 4.5.5 High Throughout Amplification and Cloning4.5.6 Sequences Identification and Analysis; 4.6 Polypeptide Toxins-Novel Applications in Drug and Pesticide Development; 4.6.1 Analgesic Drugs; 4.6.2 Insecticides; 4.7 Concluding Remarks; 4.7.1 RACE-PCR Issues; 4.7.2 Sequence Quality Issues; 4.7.3 Conclusion; 5 Wheat Antimicrobial Peptides; 5.1 Introduction; 5.2 Materials and Methods; 5.2.1 Isolation of AMPs; 5.2.2 Reduction and Alkylation of Peptides [35]; 5.2.3 MALDI-TOF MS; 5.2.4 High-Resolution Two-Dimensional Gel Electrophoresis; 5.3 Isolation of AMPs from T. kiharae Seeds 5.4 Sequence Determination of AMPs from T. kiharae Seeds |
| Record Nr. | UNINA-9910145556503321 |
| Hoboken, N.J., : Wiley-Interscience, c2008 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Peptidomics : methods and applications / / edited by Mikhail Soloviev, Chris Shaw, Per Andren
| Peptidomics : methods and applications / / edited by Mikhail Soloviev, Chris Shaw, Per Andren |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Interscience, c2008 |
| Descrizione fisica | 1 online resource (432 p.) |
| Disciplina | 612/.015756 |
| Altri autori (Persone) |
SolovievMikhail <1965->
ShawChris <1954-> AndrenPer |
| Soggetto topico |
Peptides
Proteomics |
| ISBN |
1-281-22166-X
9786611221669 0-470-19650-5 0-470-19649-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
PEPTIDOMICS; CONTENTS; Preface; Contributors; PART I STUDIES OF NATURALLY OCCURRING PEPTIDES; 1 Analysis of the Peptidomes of Amphibian Skin Granular Gland Secretions-An Integrated Functional Genomic Strategy; 1.1 Introduction; 1.2 Historical Perspective; 1.3 Contemporary Methods of Sample Acquisition; 1.4 The Integrated Functional Genomic Strategy; 1.5 How then can All Three Approaches be Integrated?; 1.6 Limitations of Each Approach; 1.7 Closing Thoughts
2 A Short History of Insect (Neuro)Peptidomics-A Personal Story of the Birth and Youth of an Excellent Model For Studying Peptidome Biology2.1 Introduction; 2.2 History; 2.3 Present (and Future): Novel Technologies, New Data. . .; 2.3.1 MALDI and (Nano)ESI TOFs and QTOFs; 2.3.2 Peptide Displays; 2.3.3 Ion Traps and FT-ICRs: Greater Variety of Instruments, Greater Variety of Sequences. . .; 2.3.4 Orbitrap; 2.3.5 FT-ICRs; 2.3.6 Combining Peptide Chemical Analysis with Histological Localization: Peptidome Mass Spectrometry Imaging; 2.4 Concluding Remarks 3 Peptidomics of Short Linear Cytolytic Peptides from Spider Venom3.1 Introduction; 3.2 Peptidomics: Identifying and Sequencing Novel AMPs; 3.3 Genomics: Unraveling the Biosynthetic Pathway; 3.4 Venomics: AMPs in the Spider Venom; 3.4.1 Structural Features; 3.4.2 Functional Features; 3.4.3 Venomic Significance; 4 Molecular Cloning Approaches to Peptidomics: The Identification of Novel cDNAs Encoding Neurotoxin-like Peptide Pools; 4.1 Introduction; 4.2 Spider Toxins-Combinatorial Peptide Libraries; 4.3 EST Cloning Approach to Cloning Peptide Families 4.4 PCR-Based Amplification and Cloning Approaches4.5 The Design and Use of Hybrid Primers for the Amplification of cDNAs Coding for Large Toxin-like Peptide Pools; 4.5.1 Multiple Alignment of the Existing Toxins and Toxin-like Sequences; 4.5.2 The Design of Hybrid Partially Degenerate Primers; 4.5.2.1 The Design of the 3 ́ Region; 4.5.2.2 The Design of the 5 ́ Region and the Middle Part of the Primers; 4.5.2.3 The Overall Length and Degeneracy; 4.5.2.4 Annealing Temperature Matching; 4.5.3 Optimization of the Amplification Conditions; 4.5.4 Other Experimental Details 4.5.5 High Throughout Amplification and Cloning4.5.6 Sequences Identification and Analysis; 4.6 Polypeptide Toxins-Novel Applications in Drug and Pesticide Development; 4.6.1 Analgesic Drugs; 4.6.2 Insecticides; 4.7 Concluding Remarks; 4.7.1 RACE-PCR Issues; 4.7.2 Sequence Quality Issues; 4.7.3 Conclusion; 5 Wheat Antimicrobial Peptides; 5.1 Introduction; 5.2 Materials and Methods; 5.2.1 Isolation of AMPs; 5.2.2 Reduction and Alkylation of Peptides [35]; 5.2.3 MALDI-TOF MS; 5.2.4 High-Resolution Two-Dimensional Gel Electrophoresis; 5.3 Isolation of AMPs from T. kiharae Seeds 5.4 Sequence Determination of AMPs from T. kiharae Seeds |
| Record Nr. | UNINA-9910814904803321 |
| Hoboken, N.J., : Wiley-Interscience, c2008 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||