Manual of Molecular and Clinical Laboratory Immunology : 2 Volume Set

• Autore: Schmitz John L
• Pubblicazione: Newark : , : ASM Press, , 2024; ©2025
• Edizione: 9th ed.
• Descrizione fisica: 1 online resource (3222 pages)
• Disciplina: 616.07/9
• Soggetto topico: Immunologic Techniques; Immunologic Tests - methods
• Soggetto genere / forma: Laboratory Manual
• Altri autori: DetrickBarbara O'GormanMaurice R. G
• Nota di contenuto: Cover -- Volume 1 -- Title Page -- Copyright Page -- Contents -- Editorial Board -- Contributors -- Preface -- Author and Editor Conflicts of interest -- Section A Molecular Methods -- Chapter 1 Introduction -- References -- Chapter 2 Molecular Methods for Genetic Diagnosis of Inborn Errors of Immunity -- Introduction -- Sequencing Technique Progression -- Sanger Sequencing -- High-Throughput Sequencing -- Pre- and postsequencing Considerations -- Sample Selection -- Analysis of variants -- Additional Techniques -- Copy Number Variation -- Trecs -- Droplet Digital PCR -- Future Considerations -- Acknowledgments -- References -- Chapter 3 Immunoglobulin and T-cell Receptor Gene Assessment -- Introduction -- Protein Structure of immunoglobulin -- Basic Immunoglobulin Structure -- Heavy-chain Isotypes -- Structure of the B-cell Receptor -- Protein Structure of the T-cell Receptor (tcr) -- T- and B-cell Development -- T-cell Development -- B Cell Development -- Genetic Structure of Immunoglobulins and t-cell Receptor -- Immunoglobulin Genes -- T-cell Receptor Genes -- Immunoglobulin and t-cell Receptor Gene Rearrangement -- B- and T-cell Receptor Gene Rearrangement Mechanism -- CDR3 Diversity -- J (joining) Region -- C (constant) Region -- Recombination Signal Sequences -- IGHC Class Switching -- Somatic Hypermutation -- Productive/unproductive Rearrangement -- Molecular Methods for TCR and IG Rearrangement Testing -- PCR-based Assays -- NGS-based Assays -- Considerations In assay Design -- Quality Controls for NGS Clonality Assays -- Interpretation of TCR and IG Rearrangement Testing -- Clonality Diagnostics and MRD Testing -- MRD Diagnostics Using Cell-free DNA -- Conclusions -- References -- Section B Immunoglobulin Methods -- Chapter 4 Introduction -- References. ; Chapter 5 Clinical Indications and applications of Serum and Urine Protein Electrophoresis -- Background on protein Structure -- Principles of Protein Electrophoresis -- Zone Electrophoresis -- Quality Control and quality Assurance -- Specimen Requirements for Serum -- Specimen Requirements for Urine -- Internal Controls -- Proteins Identified In Serum Protein Electrophoresis -- Pattern Interpretation In Serum -- Liver Disease Pattern -- Protein Loss Patterns -- Acute-phase Reaction -- Detection of M-proteins In Serum -- Quantification of Serum M-proteins -- Pattern Interpretation In Urine -- Detection of M-Proteins In the Urine -- Clinical Applications -- Avoiding False-positive Results -- Correlation of current With previous Patient Results -- References -- Chapter 6 Immunochemical Characterization of immunoglobulins In Serum, Urine, and cerebrospinal Fluid -- Background -- Laboratory Investigation -- Immunofixation -- Immunosubtraction -- Pattern Interpretations and clinical Disorders -- Polyclonal -- Endogenous Monoclonal Proteins -- Monoclonal Gammopathies -- Diseases With monoclonal Immunoglobulin Deposition -- Heavy Chain Diseases -- Plasma Cell Neoplasms -- Exogenous (therapeutic) Monoclonal Proteins and chimeric Proteins -- Oligoclonal Banding -- Hypogammaglobulinemia -- Artifacts -- Quality Control -- Urine Immunofixation -- Cerebrospinal Fluid -- Laboratory Investigation -- References -- Chapter 7 Cryoglobulins and Cryofibrinogenemia -- Cryoglobulins -- Background -- Concept -- Procedures -- Isolation, Quantitation, and characterization of cryoglobulins -- Assays for specific Antibody Activities, Antigens, or Detection of nucleic Acid -- Complement Measurements In cryoglobulinemia -- Conclusions -- Intralaboratory Quality Control and interlaboratory Proficiency Testing -- Cryofibrinogens -- Background -- Concept -- Interpretation. ; Procedures -- Conclusions -- Anticoagulant-related Recommendations -- References -- Chapter 8 Strategy for detecting and following Monoclonal Gammopathies -- Classification of monoclonal Gammopathies -- Malignant Plasma Cell Proliferative Disorders -- Premalignant Plasma Cell Proliferative Diseases -- Protein (or Low-tumor-burden) Diseases -- Overview of laboratory Tests for monoclonal Gammopathies -- Diagnostic Testing Strategies for M-Proteins In symptomatic Patients -- Monitoring M-proteins -- Stratification of risk -- Summary -- References -- Section C Complement -- Chapter 9 Introduction -- References -- Chapter 10 Methods of complement Analysis -- Introduction -- Preanalytical Considerations -- Diagnostic Workup -- Methods of modern Complement Analysis -- Functional Assays -- Protein Quantification of individual Components -- Analysis of complement Activation Products -- Multiplex Analysis of complement Proteins -- Monitoring of complement-targeted Drugs -- Complement Analysis In tissues -- Quality Assessment -- Outlook -- References -- Chapter 11 Assessing Complement Dysregulation In the Thrombotic Microangiopathies and ultrarare Complement-mediated Kidney Disease -- Alternative Pathway of complement -- Complement-mediated Renal Disease -- Atypical Hemolytic Uremic Syndrome -- C3 Glomerulopathy -- Conclusions -- Acknowledgements -- References -- Chapter 12 Current and future of complement Therapeutics and their Impact On the Clinical Immunology Laboratory -- C1-Inihibitor Concentrates -- Anti-C5 Antibodies -- Eculizumab and ABP959 -- Ravulizumab -- Crovalimab -- Pozelimab -- Anti-C5a Antibody -- Anti-masp-2 Antibody -- Cyclic Peptide C3 inhibitor -- Small-molecule C5a Receptor Antagonist -- Anti-C1 Antibodies -- Oral Small-molecule Inhibitors -- Pegylated RNA Aptamers -- RNA Interference -- Adeno-associated Virus (AAV) Gene therapy. ; References -- Section D Flow Cytometry -- Chapter 13 Introduction: Clinical Flow Cytometry Applications and quality Control -- References -- Chapter 14 Polychromatic Flow Cytometry -- Technology -- Instrumentation Overview -- Lasers -- Optical Filters -- Signal Processing and configuration of instrument Detectors -- Flow Cytometer Standardization -- Sample Preparation -- Sample Types and requirements -- Reagents -- Procedures -- Panel Design, One Approach -- Doublet Exclusion -- Quality Assurance and quality Control -- Requirements for validation of an Assay Include: -- Data Analysis and interpretation -- Future Directions -- References -- Chapter 15 High-sensitivity Detection of Paroxysmal Nocturnal Hemoglobinuria by Multiparameter Flow Cytometry -- Introduction -- Clinical Features of pnh -- Who Should Be tested for pnh? -- Complement Blockade Therapy and disease Monitoring In pnh -- Early Methods To test for pnh -- Evolution of flow Cytometric Methods To detect Pnh -- Issues With early Flow Methods -- Routine Versus High-sensitivity Flow Cytometry -- High-sensitivity Assays for pnh Red and white Blood Cells -- General Requirements -- High-sensitivity 2-c Assay for pnh In mature Rbcs -- Antibody Clone/conjugate Selection for High-sensitivity Detection of pnh MRBCs -- Instrument Setup Considerations for MRBCs -- MRBC Staining Protocol Using CD235a FITC/CD59 Pe Cocktail -- Issues With MRBC Assays -- Assays for pnh Within Immature Rbcs -- Cross-platform 3-color (3-c) Assay for pnh Irbcs and MRBCs -- Validation of 3-color Irbc/MRBC Assay To screen Pnh Samples -- High-sensitivity 4-Color (4-C) WBC assays -- Antibody Clone/conjugate Selection for 4-C High-sensitivity Pnh WBC Detection -- Instrument Setup Considerations: WBCs -- WBC Staining Procedure for optimized Neutrophil and monocyte Cocktails -- High-sensitivity 5-Color (5-C) WBC assays. ; Antibody Clone/conjugate Selection for 5-C High-sensitivity Pnh WBC Detection -- Instrument Setup Considerations for 5-Color WBC assay -- Staining Procedure for 5-Color High-sensitivity Combined Neutrophil/monocyte Assay -- Presence of type Ii Populations In neutrophils and monocytes -- CD157-negative Cases -- High-sensitivity 6-Color (6-C) WBC assays -- Antibody Clone/conjugate Selection for 6-C High-sensitivity Pnh WBC Detection -- IS FLAER Essential for high-sensitivity Testing of pnh WBCs? -- Cross-platform 5-, 6-, and 7-color Single Tube Assays for pnh WBCs -- Considerations for verification of high-sensitivity Pnh Assays -- Verification of instrument Setup and antibody Performance -- Internal Controls To verify Instrument Setup and antibody Conjugate Performance -- Fluorescence-minus-two (fm2) Controls To verify Instrument Settings -- Routine Control of assay Quality -- Considerations for validation of high-sensitivity Pnh Assays -- Ongoing QC and qc Monitors -- Reporting -- References -- Chapter 16 Standardized Flow Cytometry Assays for Enumerating CD34+ Hematopoietic Stem Cells -- Introduction -- Hematopoietic Stem/progenitor Cells Express CD34 Antigen -- Transplantation of hematopoietic Stem Cells -- Measuring Graft Adequacy -- Graft Assessment by CD34+ Cell Enumeration and Flow Cytometry -- Clinical Issues In enumerating Stem Cells by Flow Cytometry -- Early Methods to Measure CD34+ Cells by Flow Cytometry -- Statistical Issues In rare Event Detection -- Appropriate Controls for rare Event Detection -- Technical Issues in CD34+ Cell Enumeration by Multiparameter Flow Cytometry -- CD34 Antigen: Structural Considerations and choice of fluorochromes -- CD45 Antigen: Structural Considerations and choice of fluorochromes -- CD34+ Cell Enumeration Using Sequential Boolean Gating -- The Basic Ishage Protocol and gating Strategy. ; Single-platform Absolute CD34+ Cell Counting.
• Sommario/riassunto: "THE authoritative guide for clinical laboratory immunology .For over 40 years the Manual of Molecular and Clinical Laboratory Immunology has served as the premier guide for the clinical immunology laboratory. From basic serology testing to the present wide range of molecular analyses, the Manual has reflected the exponential growth in the field of immunology over the past decades. This eighth edition reflects the latest advances and developments in the diagnosis and treatment of patients with infectious and immune-mediated disorders. The Manual features detailed descriptions of general and specific methodologies, placing special focus on the interpretation of laboratory findings, and covers the immunology of infectious diseases, including specific pathogens, as well as the full range of autoimmune and immunodeficiency diseases, cancer, and transplantation. Written to guide the laboratory director, the Manual will also appeal to other laboratory scientists, especially those working in clinical immunology laboratories, and pathologists. It is also a useful reference for physicians, mid-level providers, medical students, and allied health students with an interest in the role that immunology plays in the clinical laboratory"--
• Titolo autorizzato: Manual of Molecular and Clinical Laboratory Immunology
• ISBN: 9781683674023; 1683674022; 9781683674016; 1683674014; 9781683674009; 1683674006
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9911019317303321
• Lo trovi qui: Univ. Federico II
• Serie: ASM Bks.