Immunology : overview and laboratory manual / / Tobili Y. Sam-Yellowe

• Autore: Sam-Yellowe Tobili Y.
• Pubblicazione: Cham, Switzerland : , : Springer, , [2021]; ©2021
• Edizione: 1st ed. 2021.
• Descrizione fisica: 1 online resource (xx, 471 pages) : colour illustrations
• Disciplina: 616.079
• Soggetto topico: Immunology; Immunologia; Manuals de laboratori
• Soggetto genere / forma: Llibres electrònics
• Nota di contenuto: Part I: Overview of Immunity -- Ch 1: Immunity -- Ch 2: Components of immunity -- Ch 3: Hematopoiesis -- Ch 4: The Immune system: pathogen sensing and detection -- Ch 5: Lymphoid organs -- Ch 6: Innate and Adaptive immunity -- Ch 7: Leukocyte homing, migration and recirculation -- Ch 8: Apoptosis -- Ch 9: Antigens -- Ch 10: B cell development, activation and immunoglobulin structure -- Ch 11: Immunoglobulin and T cell receptor gene rearrangements -- Ch 12: Major histocompatibility complex proteins -- Ch 13: T cell development and T cell receptor structure -- Ch 14: Cytokines -- Ch 15: Lymphocyte signals -- Ch 16: Complement fixation -- Ch 17: Cell mediated immunity -- Ch 18: Hypersensitivities -- Ch 19: Parasite Immunity -- Ch 20: Vaccines -- Ch 21: Transplantation -- Ch 22: Immune regulation and autoimmunity -- Ch 23: Immunodeficiencies -- Ch 24: Cancer -- Ch 25: Practice questions -- Ch 26: Study guide for exam. Part II. Laboratory Manual --Ch 27. Immunology laboratory policies -- Ch 28. Instructions for notebook entries -- Ch 29. Instructions for writing lab reports -- Ch 30. Lab report topics -- Ch 31. Exercise 1: Using a compound microscope -- Ch 32. Exercise 2: Pipetting and dilution technique, identification of mouse lymphoid organs -- Ch 33. Exercise 3: Blood Cell Preparation, Leukocyte differentiation and ABO Blood Typing -- Ch 34. Exercise 4: Single Suspension of Mouse Spleen cells, Cell Viability Assays, and Identification of specific cells using surface antigens -- Ch 35. Exercise 5: Isolation of mouse peritoneal macrophages: In vitro phagocytosis of bacterial cells -- Ch 36. Exercise 6: Clearance of bacteria from mouse blood by the RES -- Ch 37. Exercise 7: Hemolytic Plaque assay (The Jeme Plaque Assay) -- Ch 38. EXERCISE 8: Rabbit and Mouse Immunizations- Preparation of Polyclonal antibodies and screening assays -- Ch 39. EXERCISE 9: Determination of Antibody Titer: Screening Techniques -- Ch 40. EXERCISE 10: Immunodiffusion and Immunoelectrophoresis -- Ch 41. EXERCISE 11: Protein Affinity Purification of Rabbit IgG -- Ch 42. EXERCISE 12: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) -- Ch 43. EXERCISE 13: Immunoblotting (Western Blotting) -- Ch 44. Exercise 14: Protein Assay -- Ch 45. Exercise 15: Enzyme-linked Immunosorbent Assay (ELISA) -- Ch 46. EXERCISE 16: Enzyme-Linked Immunospot Assay (ELISPOT) -- Ch 47. EXERCISE 17: Immunofluorescence assay (IFA) -- Ch 48. EXERCISE 18: Flow Cytometry -- Ch 49.EXERCISE 19: Complement Fixation Assays -- Ch 50. Tissue Cell Culture Techniques -- Ch 51. EXERCISE 20: Mitogen Induced Response of Lymphocytes: Detection of Band T cell Lymphocytes Using Immunofluorescence -- Ch 52. Exercise 21: Induction of IL-2 Secretion from Mouse Lymphoma Cells -- Ch 53. EXERCISE 22: Adoptive Transfer of Lymphocytes: Band T cell Cooperation for Antibody Production -- Ch 54. EXERCISE 23: Macrophage Migration Inhibition -- Ch 55. EXERCISE 24: Monoclonal Antibody Preparation -- Ch 56. EXERCISE 25: Immunization of mice with a recombinant protein -- Ch 57. EXERCISE 26: Polarization of T helper cells into TH1 subset -- Ch 58. Exercise 27: Immunoprecipitation -- Ch 59. EXERCISE 28: Induction of apoptosis in T lymphocytes and detection of apoptotic markers -- Ch 60. EXERCISE 29: Cloning and sequencing of cDNAs of mouse variable regions of H and L chains of monoclonal antibodies.
• Sommario/riassunto: A two-in-one text providing teaching lab students with an overview of immunology as well as a lab manual complete with current standard exercises. Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. Section II consists of twenty-two lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions.
• Titolo autorizzato: Immunology
• ISBN: 3-030-64686-6
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910495233203321
• Lo trovi qui: Univ. Federico II