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Biochemistry laboratory manual for undergraduates : an inquiry-based approach / / Timea Gerczei, Scott Pattison; managing editor, Anna Rulka
| Biochemistry laboratory manual for undergraduates : an inquiry-based approach / / Timea Gerczei, Scott Pattison; managing editor, Anna Rulka |
| Autore | Gerczei Timea |
| Pubbl/distr/stampa | Warsaw, [Poland] ; ; Berlin, [Germany] : , : De Gruyter Open, , 2014 |
| Descrizione fisica | 1 online resource (186 pages) : illustrations, (some colour) |
| Disciplina | 572.078 |
| Soggetto topico |
Biochemistry
Molecular biology Drug resistance in microorganisms |
| ISBN |
1-5231-0070-2
3-11-042625-0 3-11-041133-4 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Front matter -- Preface -- Contents -- 1 Introducing the Bacterial Antibiotic Sensor Mini Project -- 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function -- 3 Designing Primers for Site-Directed Mutagenesis -- 4 Performing Site-Directed Mutagenesis -- 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy -- 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease -- 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro -- 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry -- 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching -- 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy -- 11 Understanding the Importance of Buffers in Biological Systems -- 12 Molecular Visualization of an Enzyme, Acetylcholinesterase -- 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment -- 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC -- List of Figures |
| Record Nr. | UNINA-9910132278903321 |
Gerczei Timea
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| Warsaw, [Poland] ; ; Berlin, [Germany] : , : De Gruyter Open, , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Biochemistry laboratory manual for undergraduates : an inquiry-based approach / / Timea Gerczei, Scott Pattison; managing editor, Anna Rulka
| Biochemistry laboratory manual for undergraduates : an inquiry-based approach / / Timea Gerczei, Scott Pattison; managing editor, Anna Rulka |
| Autore | Gerczei Timea |
| Pubbl/distr/stampa | Warsaw, [Poland] ; ; Berlin, [Germany] : , : De Gruyter Open, , 2014 |
| Descrizione fisica | 1 online resource (186 pages) : illustrations, (some colour) |
| Disciplina | 572.078 |
| Soggetto topico |
Biochemistry
Molecular biology Drug resistance in microorganisms |
| ISBN |
1-5231-0070-2
3-11-042625-0 3-11-041133-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Front matter -- Preface -- Contents -- 1 Introducing the Bacterial Antibiotic Sensor Mini Project -- 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function -- 3 Designing Primers for Site-Directed Mutagenesis -- 4 Performing Site-Directed Mutagenesis -- 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy -- 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease -- 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro -- 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry -- 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching -- 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy -- 11 Understanding the Importance of Buffers in Biological Systems -- 12 Molecular Visualization of an Enzyme, Acetylcholinesterase -- 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment -- 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC -- List of Figures |
| Record Nr. | UNISA-996308751403316 |
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Gerczei Timea
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| Warsaw, [Poland] ; ; Berlin, [Germany] : , : De Gruyter Open, , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
Biomedical chemistry : current trends and developments / / Nuno Vale ; managing editor, Anna Rulka ; language editor, Reuben Hudson & Michael Jones
| Biomedical chemistry : current trends and developments / / Nuno Vale ; managing editor, Anna Rulka ; language editor, Reuben Hudson & Michael Jones |
| Autore | Vale Nuno |
| Pubbl/distr/stampa | Berlin, [Germany] : , : De Gruyter Open, , 2015 |
| Descrizione fisica | 1 online resource (361 pages) : illustrations, tables |
| Disciplina | 615.19 |
| Soggetto topico | Pharmaceutical chemistry |
| Soggetto genere / forma | Electronic books. |
| Soggetto non controllato | medicinal chemistry, natural products, drug design, drug discovery, translational research |
| ISBN |
3-11-046875-1
3-11-046887-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Front matter -- Contents -- Preface -- Section 1: Chemical Principles in Drug Design and Discovery -- 1.1 Functional Groups of Biomolecules and their Reactions / Pinto, Diana C. G. A. / Pereira, João M. P. / Silva, Artur M. S. -- 1.2 Designing Covalent Inhibitors: A Medicinal Chemistry Challenge / Lopes, Francisca / Santos, Maria M. M. / Moreira, Rui -- Section 2: Chemical Basis of Drug Action and Diseases -- 2.1 Pharmacokinetics and Bioanalysis to Improve Drug Development / Fortuna, Ana / Alves, Gilberto / Falcão, Amílcar -- 2.2 Translational Research in Endocrinology and Neuroimmunology Applied to Depression / Wong, Ma-Li / Lewis, Martin / Licinio, Julio -- 2.3 Understanding the Metabolic Syndrome Using a Biomedical Chemistry Profile / Pereira, Cidália / Monteiro, Rosário / João Martins, Maria -- 2.4 Brain Neurochemistry and Cognitive Performance: Neurotransmitter Systems / Lobo, Andrea / Summavielle, Teresa -- Section 3: Strategies to Develop New and Better Drugs -- 3.1 Amino Acids and Peptides in Medicine: Old or New Drugs? / Vale, Nuno -- 3.2 Targeting Calcium-mediated Excitotoxicity in the CNS / Chambers, James J. -- 3.3 Strategies for Conversion of Peptides to Peptidomimetic Drugs / Fransson, Rebecca / Sköld, Christian / Sandström, Anja -- 3.4 Synthetic Vs. Natural Bioactive Compounds Against Tropical Disease / Pybus, Brandon S. -- 3.5 Current Trends and Developments for Nanotechnology in Cancer / Ferreira, Mónica / Almeida, Patrick / Shahbazi, Mohammad-Ali / Correia, Alexandra / Santos, Hélder A. -- Index |
| Record Nr. | UNINA-9910160768303321 |
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Vale Nuno
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| Berlin, [Germany] : , : De Gruyter Open, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Biomedical chemistry : current trends and developments / / Nuno Vale ; managing editor, Anna Rulka ; language editor, Reuben Hudson & Michael Jones
| Biomedical chemistry : current trends and developments / / Nuno Vale ; managing editor, Anna Rulka ; language editor, Reuben Hudson & Michael Jones |
| Autore | Vale Nuno |
| Pubbl/distr/stampa | Berlin, [Germany] : , : De Gruyter Open, , 2015 |
| Descrizione fisica | 1 online resource (361 pages) : illustrations, tables |
| Disciplina | 615.19 |
| Soggetto topico | Pharmaceutical chemistry |
| Soggetto genere / forma | Electronic books. |
| Soggetto non controllato | medicinal chemistry, natural products, drug design, drug discovery, translational research |
| ISBN |
3-11-046875-1
3-11-046887-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Front matter -- Contents -- Preface -- Section 1: Chemical Principles in Drug Design and Discovery -- 1.1 Functional Groups of Biomolecules and their Reactions / Pinto, Diana C. G. A. / Pereira, João M. P. / Silva, Artur M. S. -- 1.2 Designing Covalent Inhibitors: A Medicinal Chemistry Challenge / Lopes, Francisca / Santos, Maria M. M. / Moreira, Rui -- Section 2: Chemical Basis of Drug Action and Diseases -- 2.1 Pharmacokinetics and Bioanalysis to Improve Drug Development / Fortuna, Ana / Alves, Gilberto / Falcão, Amílcar -- 2.2 Translational Research in Endocrinology and Neuroimmunology Applied to Depression / Wong, Ma-Li / Lewis, Martin / Licinio, Julio -- 2.3 Understanding the Metabolic Syndrome Using a Biomedical Chemistry Profile / Pereira, Cidália / Monteiro, Rosário / João Martins, Maria -- 2.4 Brain Neurochemistry and Cognitive Performance: Neurotransmitter Systems / Lobo, Andrea / Summavielle, Teresa -- Section 3: Strategies to Develop New and Better Drugs -- 3.1 Amino Acids and Peptides in Medicine: Old or New Drugs? / Vale, Nuno -- 3.2 Targeting Calcium-mediated Excitotoxicity in the CNS / Chambers, James J. -- 3.3 Strategies for Conversion of Peptides to Peptidomimetic Drugs / Fransson, Rebecca / Sköld, Christian / Sandström, Anja -- 3.4 Synthetic Vs. Natural Bioactive Compounds Against Tropical Disease / Pybus, Brandon S. -- 3.5 Current Trends and Developments for Nanotechnology in Cancer / Ferreira, Mónica / Almeida, Patrick / Shahbazi, Mohammad-Ali / Correia, Alexandra / Santos, Hélder A. -- Index |
| Record Nr. | UNISA-996308772703316 |
|
Vale Nuno
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||
| Berlin, [Germany] : , : De Gruyter Open, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
Miniaturization in sample preparation / / Francisco Pena-Pereira, [editor]; managing editor, Anna Rulka; language editor Perry Mitchell
| Miniaturization in sample preparation / / Francisco Pena-Pereira, [editor]; managing editor, Anna Rulka; language editor Perry Mitchell |
| Autore | Pena Pereira Francisco |
| Pubbl/distr/stampa | Warsaw, [Poland] ; ; Berlin, [Germany] : , : De Gruyter Open, , 2014 |
| Descrizione fisica | 1 online resource (464 pages) |
| Disciplina | 543 |
| Soggetto topico |
Sampling
Sample preparation (Chemistry) Extraction (Chemistry) |
| Soggetto genere / forma | Electronic books. |
| ISBN |
3-11-042726-5
3-11-041018-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Front matter -- Contents -- List of Contributors -- 1 From Conventional to Miniaturized Analytical Systems / Pena-Pereira, Francisco -- 2 Solid-Phase Microextraction and Related Techniques / Bagheri, Habib / Piri-Moghadam, Hamed / Naderi, Mehrnoush / Es'haghi, Ali / Roostaie, Ali -- 3 Novel Materials in Solid-Phase Microextraction and Related Sample Preparation Approaches / Hu, Bin / He, Man / Chen, Beibei -- 4 Liquid-phase Microextraction Techniques / Fernández, Elena / Vidal, Lorena -- 5 Choice of Solvent in Liquid-Phase Microextraction / Dadfarnia , Shayessteh / Haji-Shabani, Ali Mohammad -- 6 Method Development with Miniaturized Sample Preparation Techniques / Costas-Rodriguez, Marta / Pena-Pereira, Francisco -- 7 Miniaturized Alternatives to Conventional Sample Preparation Techniques for Solid Samples / Cabaleiro, Noelia / de la Calle, Inmaculada -- 8 Green Aspects of Miniaturized Sample Preparation Techniques / Kloskowski, Adam / Marcinkowski, Łukasz / Namieśnik, Jacek -- Index |
| Record Nr. | UNINA-9910132279803321 |
|
Pena Pereira Francisco
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||
| Warsaw, [Poland] ; ; Berlin, [Germany] : , : De Gruyter Open, , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Miniaturization in sample preparation / / Francisco Pena-Pereira, [editor]; managing editor, Anna Rulka; language editor Perry Mitchell
| Miniaturization in sample preparation / / Francisco Pena-Pereira, [editor]; managing editor, Anna Rulka; language editor Perry Mitchell |
| Autore | Pena Pereira Francisco |
| Pubbl/distr/stampa | Warsaw, [Poland] ; ; Berlin, [Germany] : , : De Gruyter Open, , 2014 |
| Descrizione fisica | 1 online resource (464 pages) |
| Disciplina | 543 |
| Soggetto topico |
Sampling
Sample preparation (Chemistry) Extraction (Chemistry) |
| Soggetto genere / forma | Electronic books. |
| ISBN |
3-11-042726-5
3-11-041018-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Front matter -- Contents -- List of Contributors -- 1 From Conventional to Miniaturized Analytical Systems / Pena-Pereira, Francisco -- 2 Solid-Phase Microextraction and Related Techniques / Bagheri, Habib / Piri-Moghadam, Hamed / Naderi, Mehrnoush / Es'haghi, Ali / Roostaie, Ali -- 3 Novel Materials in Solid-Phase Microextraction and Related Sample Preparation Approaches / Hu, Bin / He, Man / Chen, Beibei -- 4 Liquid-phase Microextraction Techniques / Fernández, Elena / Vidal, Lorena -- 5 Choice of Solvent in Liquid-Phase Microextraction / Dadfarnia , Shayessteh / Haji-Shabani, Ali Mohammad -- 6 Method Development with Miniaturized Sample Preparation Techniques / Costas-Rodriguez, Marta / Pena-Pereira, Francisco -- 7 Miniaturized Alternatives to Conventional Sample Preparation Techniques for Solid Samples / Cabaleiro, Noelia / de la Calle, Inmaculada -- 8 Green Aspects of Miniaturized Sample Preparation Techniques / Kloskowski, Adam / Marcinkowski, Łukasz / Namieśnik, Jacek -- Index |
| Record Nr. | UNISA-996309149103316 |
|
Pena Pereira Francisco
|
||
| Warsaw, [Poland] ; ; Berlin, [Germany] : , : De Gruyter Open, , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
