Info
- Utilizzare la checkbox di selezione a fianco di ciascun documento per attivare le funzionalità di stampa, invio email, download nei formati disponibili del (i) record.
Enzyme assays [[electronic resource] ] : high-throughput screening, genetic selection, and fingerprinting / / edited by Jean-Louis Reymond
| Enzyme assays [[electronic resource] ] : high-throughput screening, genetic selection, and fingerprinting / / edited by Jean-Louis Reymond |
| Pubbl/distr/stampa | Weinheim, Germany, : Wiley-VCH, c2006 |
| Descrizione fisica | 1 online resource (388 p.) |
| Disciplina | 572.7 |
| Altri autori (Persone) | ReymondJean-Louis |
| Soggetto topico | Enzymes - Analysis |
| ISBN |
1-280-85428-6
9786610854288 3-527-60784-6 3-527-60721-8 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Enzyme Assays; Contents; Preface; List of Contributors; Introduction; Enzyme Assays; Part I: High-throughput Screening; Part II: Genetic Selection; Part III: Enzyme Fingerprinting; Enzyme Assays in Other Areas; How to Use this Book; Part I High-throughput Screening; 1 Quantitative Assay of Hydrolases for Activity and Selectivity Using Color Changes; 1.1 Overview; 1.2 Direct Assays Using Chromogenic Substrates; 1.3 Indirect Assays Using Coupled Reactions - pH Indicators; 1.3.1 Overview of Quantitative Use of pH Indicator Assay; 1.3.2 Applications
1.3.2.1 Searching for an Active Hydrolase (Testing Many Hydrolases Toward One Substrate)1.3.2.2 Substrate Mapping of New Hydrolases (Testing Many Substrates Toward Hydrolase); 1.3.3 Comparison with Other Methods; 1.4 Estimating and Measuring Selectivity; 1.4.1 Estimating Selectivity without a Reference Compound; 1.4.2 Quantitative Measure of Selectivity Using a Reference Compound (Quick E and Related Methods); 1.4.2.1 Chromogenic Substrate; 1.4.2.2 pH Indicators; 1.4.3 Application; 1.4.3.1 Substrate Mapping of Hydrolases; 1.4.3.2 Screening of Mutants in Directed Evolution 1.4.4 Advantages and DisadvantagesReferences; 2 High-throughput Screening Systems for Assaying the Enantioselectivity of Enzymes; 2.1 Introduction; 2.2 UV/Vis Spectroscopy-based Assays; 2.2.1 Assay for Screening Lipases or Esterases in the Kinetic Resolution of Chiral p-Nitrophenyl Esters; 2.2.2 Enzyme-coupled UV/Vis-based Assay for Lipases and Esterases; 2.2.3 Enzymatic Method for Determining Enantiomeric Excess (EMDee); 2.2.4 UV/Vis-based Enzyme Immunoassay as a Means to Measure Enantiomeric Excess; 2.2.5 Other UV/Vis-based ee-Assays; 2.3 Assays Using Fluorescence 2.3.1 Umbelliferone-based Systems2.3.2 Fluorescence-based Assay Using DNA Microarrays; 2.3.3 Other Fluorescence-based ee-Assays; 2.4 Assays Based on Mass Spectrometry (MS); 2.4.1 MS-based Assay Using Isotope Labeling; 2.5 Assays Based on Nuclear Magnetic Resonance Spectroscopy; 2.6 Assay Based on Fourier Transform Infrared Spectroscopy for Assaying Lipases or Esterases; 2.7 Assays Based on Gas Chromatography; 2.8 Assays Based on HPLC; 2.9 Assays Based on Capillary Array Electrophoresis; 2.10 Assays Based on Circular Dichroism (CD) 2.11 Assay Based on Surface-enhanced Resonance Raman Scattering2.12 Conclusions; References; 3 High-throughput Screening Methods Developed for Oxidoreductases; 3.1 Introduction; 3.2 High-throughput Methods for Various Oxidoreductases; 3.2.1 Dehydrogenases; 3.2.1.1 Colorimetric Screen Based on NAD(P)H Generation; 3.2.1.2 Screens Based on NAD(P)H Depletion; 3.2.2 Oxidases; 3.2.2.1 Galactose Oxidase; 3.2.2.2 D-Amino Acid Oxidase; 3.2.2.3 Peroxidases; 3.2.3 Oxygenases; 3.2.3.1 Assays Based on Optical Properties of Substrates and Products 3.2.3.2 Assays Based on Gibbs' Reagent and 4-Aminoantipyrine |
| Record Nr. | UNINA-9910143962403321 |
| Weinheim, Germany, : Wiley-VCH, c2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Enzyme assays [[electronic resource] ] : high-throughput screening, genetic selection, and fingerprinting / / edited by Jean-Louis Reymond
| Enzyme assays [[electronic resource] ] : high-throughput screening, genetic selection, and fingerprinting / / edited by Jean-Louis Reymond |
| Pubbl/distr/stampa | Weinheim, Germany, : Wiley-VCH, c2006 |
| Descrizione fisica | 1 online resource (388 p.) |
| Disciplina | 572.7 |
| Altri autori (Persone) | ReymondJean-Louis |
| Soggetto topico | Enzymes - Analysis |
| ISBN |
1-280-85428-6
9786610854288 3-527-60784-6 3-527-60721-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Enzyme Assays; Contents; Preface; List of Contributors; Introduction; Enzyme Assays; Part I: High-throughput Screening; Part II: Genetic Selection; Part III: Enzyme Fingerprinting; Enzyme Assays in Other Areas; How to Use this Book; Part I High-throughput Screening; 1 Quantitative Assay of Hydrolases for Activity and Selectivity Using Color Changes; 1.1 Overview; 1.2 Direct Assays Using Chromogenic Substrates; 1.3 Indirect Assays Using Coupled Reactions - pH Indicators; 1.3.1 Overview of Quantitative Use of pH Indicator Assay; 1.3.2 Applications
1.3.2.1 Searching for an Active Hydrolase (Testing Many Hydrolases Toward One Substrate)1.3.2.2 Substrate Mapping of New Hydrolases (Testing Many Substrates Toward Hydrolase); 1.3.3 Comparison with Other Methods; 1.4 Estimating and Measuring Selectivity; 1.4.1 Estimating Selectivity without a Reference Compound; 1.4.2 Quantitative Measure of Selectivity Using a Reference Compound (Quick E and Related Methods); 1.4.2.1 Chromogenic Substrate; 1.4.2.2 pH Indicators; 1.4.3 Application; 1.4.3.1 Substrate Mapping of Hydrolases; 1.4.3.2 Screening of Mutants in Directed Evolution 1.4.4 Advantages and DisadvantagesReferences; 2 High-throughput Screening Systems for Assaying the Enantioselectivity of Enzymes; 2.1 Introduction; 2.2 UV/Vis Spectroscopy-based Assays; 2.2.1 Assay for Screening Lipases or Esterases in the Kinetic Resolution of Chiral p-Nitrophenyl Esters; 2.2.2 Enzyme-coupled UV/Vis-based Assay for Lipases and Esterases; 2.2.3 Enzymatic Method for Determining Enantiomeric Excess (EMDee); 2.2.4 UV/Vis-based Enzyme Immunoassay as a Means to Measure Enantiomeric Excess; 2.2.5 Other UV/Vis-based ee-Assays; 2.3 Assays Using Fluorescence 2.3.1 Umbelliferone-based Systems2.3.2 Fluorescence-based Assay Using DNA Microarrays; 2.3.3 Other Fluorescence-based ee-Assays; 2.4 Assays Based on Mass Spectrometry (MS); 2.4.1 MS-based Assay Using Isotope Labeling; 2.5 Assays Based on Nuclear Magnetic Resonance Spectroscopy; 2.6 Assay Based on Fourier Transform Infrared Spectroscopy for Assaying Lipases or Esterases; 2.7 Assays Based on Gas Chromatography; 2.8 Assays Based on HPLC; 2.9 Assays Based on Capillary Array Electrophoresis; 2.10 Assays Based on Circular Dichroism (CD) 2.11 Assay Based on Surface-enhanced Resonance Raman Scattering2.12 Conclusions; References; 3 High-throughput Screening Methods Developed for Oxidoreductases; 3.1 Introduction; 3.2 High-throughput Methods for Various Oxidoreductases; 3.2.1 Dehydrogenases; 3.2.1.1 Colorimetric Screen Based on NAD(P)H Generation; 3.2.1.2 Screens Based on NAD(P)H Depletion; 3.2.2 Oxidases; 3.2.2.1 Galactose Oxidase; 3.2.2.2 D-Amino Acid Oxidase; 3.2.2.3 Peroxidases; 3.2.3 Oxygenases; 3.2.3.1 Assays Based on Optical Properties of Substrates and Products 3.2.3.2 Assays Based on Gibbs' Reagent and 4-Aminoantipyrine |
| Record Nr. | UNINA-9910830562203321 |
| Weinheim, Germany, : Wiley-VCH, c2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Enzyme assays : high-throughput screening, genetic selection, and fingerprinting / / edited by Jean-Louis Reymond
| Enzyme assays : high-throughput screening, genetic selection, and fingerprinting / / edited by Jean-Louis Reymond |
| Pubbl/distr/stampa | Weinheim, Germany, : Wiley-VCH, c2006 |
| Descrizione fisica | 1 online resource (388 p.) |
| Disciplina | 572.76 |
| Altri autori (Persone) | ReymondJean-Louis |
| Soggetto topico | Enzymes - Analysis |
| ISBN |
1-280-85428-6
9786610854288 3-527-60784-6 3-527-60721-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Enzyme Assays; Contents; Preface; List of Contributors; Introduction; Enzyme Assays; Part I: High-throughput Screening; Part II: Genetic Selection; Part III: Enzyme Fingerprinting; Enzyme Assays in Other Areas; How to Use this Book; Part I High-throughput Screening; 1 Quantitative Assay of Hydrolases for Activity and Selectivity Using Color Changes; 1.1 Overview; 1.2 Direct Assays Using Chromogenic Substrates; 1.3 Indirect Assays Using Coupled Reactions - pH Indicators; 1.3.1 Overview of Quantitative Use of pH Indicator Assay; 1.3.2 Applications
1.3.2.1 Searching for an Active Hydrolase (Testing Many Hydrolases Toward One Substrate)1.3.2.2 Substrate Mapping of New Hydrolases (Testing Many Substrates Toward Hydrolase); 1.3.3 Comparison with Other Methods; 1.4 Estimating and Measuring Selectivity; 1.4.1 Estimating Selectivity without a Reference Compound; 1.4.2 Quantitative Measure of Selectivity Using a Reference Compound (Quick E and Related Methods); 1.4.2.1 Chromogenic Substrate; 1.4.2.2 pH Indicators; 1.4.3 Application; 1.4.3.1 Substrate Mapping of Hydrolases; 1.4.3.2 Screening of Mutants in Directed Evolution 1.4.4 Advantages and DisadvantagesReferences; 2 High-throughput Screening Systems for Assaying the Enantioselectivity of Enzymes; 2.1 Introduction; 2.2 UV/Vis Spectroscopy-based Assays; 2.2.1 Assay for Screening Lipases or Esterases in the Kinetic Resolution of Chiral p-Nitrophenyl Esters; 2.2.2 Enzyme-coupled UV/Vis-based Assay for Lipases and Esterases; 2.2.3 Enzymatic Method for Determining Enantiomeric Excess (EMDee); 2.2.4 UV/Vis-based Enzyme Immunoassay as a Means to Measure Enantiomeric Excess; 2.2.5 Other UV/Vis-based ee-Assays; 2.3 Assays Using Fluorescence 2.3.1 Umbelliferone-based Systems2.3.2 Fluorescence-based Assay Using DNA Microarrays; 2.3.3 Other Fluorescence-based ee-Assays; 2.4 Assays Based on Mass Spectrometry (MS); 2.4.1 MS-based Assay Using Isotope Labeling; 2.5 Assays Based on Nuclear Magnetic Resonance Spectroscopy; 2.6 Assay Based on Fourier Transform Infrared Spectroscopy for Assaying Lipases or Esterases; 2.7 Assays Based on Gas Chromatography; 2.8 Assays Based on HPLC; 2.9 Assays Based on Capillary Array Electrophoresis; 2.10 Assays Based on Circular Dichroism (CD) 2.11 Assay Based on Surface-enhanced Resonance Raman Scattering2.12 Conclusions; References; 3 High-throughput Screening Methods Developed for Oxidoreductases; 3.1 Introduction; 3.2 High-throughput Methods for Various Oxidoreductases; 3.2.1 Dehydrogenases; 3.2.1.1 Colorimetric Screen Based on NAD(P)H Generation; 3.2.1.2 Screens Based on NAD(P)H Depletion; 3.2.2 Oxidases; 3.2.2.1 Galactose Oxidase; 3.2.2.2 D-Amino Acid Oxidase; 3.2.2.3 Peroxidases; 3.2.3 Oxygenases; 3.2.3.1 Assays Based on Optical Properties of Substrates and Products 3.2.3.2 Assays Based on Gibbs' Reagent and 4-Aminoantipyrine |
| Record Nr. | UNINA-9910877202003321 |
| Weinheim, Germany, : Wiley-VCH, c2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||