Info
- Utilizzare la checkbox di selezione a fianco di ciascun documento per attivare le funzionalità di stampa, invio email, download nei formati disponibili del (i) record.
Molecular biology techniques [[electronic resource] ] : an intensive laboratory course / / [edited by] Walt Ream and Katharine G. Field
| Molecular biology techniques [[electronic resource] ] : an intensive laboratory course / / [edited by] Walt Ream and Katharine G. Field |
| Pubbl/distr/stampa | San Diego, CA, : Academic Press, 1998 |
| Descrizione fisica | 1 online resource (245 p.) |
| Disciplina | 572.8/078 |
| Altri autori (Persone) |
FieldKatharine G
ReamWalt |
| Soggetto topico |
Molecular biology
Molecular genetics |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-01889-9
9786611018894 0-08-053682-4 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Molecular Biology Techniques: An Intensive Laboratory Course; Copyright Page; Contents; Preface; Course Synopsis; Introduction; Safety Precautions; Daily Schedule; Acknowledgments; Exercises I. DNA Preparation, Polymerase Chain Reaction, and Molecular Cloning; A. Cesium chloride-ethidium bromide density gradient centrifugation; B. PCR to synthesize virD2 flanked with restriction sites; C. Restriction digests of plasmid pGEX2 and PCR products; D. Purification of DNA fragments from agarose; E. Ligation of PCR product to pGEX2 vector
F. Transformation of E. coli with the ligated plasmidG. Small-scale preparation of plasmid DNA by the alkaline lysis method; H. Restriction analysis; Study questions; Exercises II. Protein Expression, Purification, and Analysis; A. Expression and purification of a fusion protein; B. SDS-polyacrylamide gel electrophoresis; C. Silver stain detection of proteins; D. Western blot (immunoblot) detection of proteins; Study questions; Exercises III. Oligonucleotide-Directed Mutagenesis; A. Restriction digests of virD2 (in pCS64) and pUC119; B. Purification of DNA fragments from agarose C. Ligation of restriction fragment and vectorD. Transformation of E. coli with the ligated plasmid and recovery of clones; E. Small-scale preparation of plasmid DNA from broth cultures; F. Restriction digest of DNAs: Examination to confirm insert; G. Preparation of single-stranded DNA template; H. Phosphorylation of oligonucleotide; I. Annealing mutant oligonucleotide to template; J. In vitro DNA synthesis by primer extension; K. Transform synthesis reaction into E. coli DH5a; L. Small-scale preparation of plasmid DNA; M. Confirmation of mutants by restriction analysis; Study questions Exercises IV. DNA SequencingA. Polyacrylamide sequencing gel electrophoresis; B. Dideoxy sequencing; C. Automated sequencing; D. Introduction to databases and gene sequence analysis; Study questions; Exercises V. Southern Blot Detection of DNA; A. Preparation of genomic DNA from Agrobacterium tumefaciens; B. Restriction digestion of genomic DNA; C. Agarose gel electrophoresis of restriction fragments; D. Southern blot: Denaturation and blotting of DNA; E. Preparation of probe by nick translation; F. Hybridization and washing of Southern blots; Study questions Exercises VI. Northern Blot Detection of mRNAA. Preparation of RNA from tobacco leaves; B. Agarose-formaldehyde gel electrophoresis; C. Northern blot: Denaturation and blotting of RNA; D. Probe preparation; E. Hybridization and washing of Northern blots; Study questions; Exercises VII. Protein Interaction Analysis in Yeast; A. Yeast transformation; B. Filter ß-galactosidase assay; Index |
| Record Nr. | UNINA-9910458228103321 |
| San Diego, CA, : Academic Press, 1998 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular biology techniques [[electronic resource] ] : an intensive laboratory course / / [edited by] Walt Ream and Katharine G. Field
| Molecular biology techniques [[electronic resource] ] : an intensive laboratory course / / [edited by] Walt Ream and Katharine G. Field |
| Pubbl/distr/stampa | San Diego, CA, : Academic Press, 1998 |
| Descrizione fisica | 1 online resource (245 p.) |
| Disciplina | 572.8/078 |
| Altri autori (Persone) |
FieldKatharine G
ReamWalt |
| Soggetto topico |
Molecular biology
Molecular genetics |
| ISBN |
1-281-01889-9
9786611018894 0-08-053682-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Molecular Biology Techniques: An Intensive Laboratory Course; Copyright Page; Contents; Preface; Course Synopsis; Introduction; Safety Precautions; Daily Schedule; Acknowledgments; Exercises I. DNA Preparation, Polymerase Chain Reaction, and Molecular Cloning; A. Cesium chloride-ethidium bromide density gradient centrifugation; B. PCR to synthesize virD2 flanked with restriction sites; C. Restriction digests of plasmid pGEX2 and PCR products; D. Purification of DNA fragments from agarose; E. Ligation of PCR product to pGEX2 vector
F. Transformation of E. coli with the ligated plasmidG. Small-scale preparation of plasmid DNA by the alkaline lysis method; H. Restriction analysis; Study questions; Exercises II. Protein Expression, Purification, and Analysis; A. Expression and purification of a fusion protein; B. SDS-polyacrylamide gel electrophoresis; C. Silver stain detection of proteins; D. Western blot (immunoblot) detection of proteins; Study questions; Exercises III. Oligonucleotide-Directed Mutagenesis; A. Restriction digests of virD2 (in pCS64) and pUC119; B. Purification of DNA fragments from agarose C. Ligation of restriction fragment and vectorD. Transformation of E. coli with the ligated plasmid and recovery of clones; E. Small-scale preparation of plasmid DNA from broth cultures; F. Restriction digest of DNAs: Examination to confirm insert; G. Preparation of single-stranded DNA template; H. Phosphorylation of oligonucleotide; I. Annealing mutant oligonucleotide to template; J. In vitro DNA synthesis by primer extension; K. Transform synthesis reaction into E. coli DH5a; L. Small-scale preparation of plasmid DNA; M. Confirmation of mutants by restriction analysis; Study questions Exercises IV. DNA SequencingA. Polyacrylamide sequencing gel electrophoresis; B. Dideoxy sequencing; C. Automated sequencing; D. Introduction to databases and gene sequence analysis; Study questions; Exercises V. Southern Blot Detection of DNA; A. Preparation of genomic DNA from Agrobacterium tumefaciens; B. Restriction digestion of genomic DNA; C. Agarose gel electrophoresis of restriction fragments; D. Southern blot: Denaturation and blotting of DNA; E. Preparation of probe by nick translation; F. Hybridization and washing of Southern blots; Study questions Exercises VI. Northern Blot Detection of mRNAA. Preparation of RNA from tobacco leaves; B. Agarose-formaldehyde gel electrophoresis; C. Northern blot: Denaturation and blotting of RNA; D. Probe preparation; E. Hybridization and washing of Northern blots; Study questions; Exercises VII. Protein Interaction Analysis in Yeast; A. Yeast transformation; B. Filter ß-galactosidase assay; Index |
| Record Nr. | UNINA-9910784526803321 |
| San Diego, CA, : Academic Press, 1998 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular biology techniques : an intensive laboratory course / / [edited by] Walt Ream and Katharine G. Field
| Molecular biology techniques : an intensive laboratory course / / [edited by] Walt Ream and Katharine G. Field |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | San Diego, CA, : Academic Press, 1998 |
| Descrizione fisica | 1 online resource (245 p.) |
| Disciplina | 572.8/078 |
| Altri autori (Persone) |
FieldKatharine G
ReamWalt |
| Soggetto topico |
Molecular biology
Molecular genetics |
| ISBN |
1-281-01889-9
9786611018894 0-08-053682-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Molecular Biology Techniques: An Intensive Laboratory Course; Copyright Page; Contents; Preface; Course Synopsis; Introduction; Safety Precautions; Daily Schedule; Acknowledgments; Exercises I. DNA Preparation, Polymerase Chain Reaction, and Molecular Cloning; A. Cesium chloride-ethidium bromide density gradient centrifugation; B. PCR to synthesize virD2 flanked with restriction sites; C. Restriction digests of plasmid pGEX2 and PCR products; D. Purification of DNA fragments from agarose; E. Ligation of PCR product to pGEX2 vector
F. Transformation of E. coli with the ligated plasmidG. Small-scale preparation of plasmid DNA by the alkaline lysis method; H. Restriction analysis; Study questions; Exercises II. Protein Expression, Purification, and Analysis; A. Expression and purification of a fusion protein; B. SDS-polyacrylamide gel electrophoresis; C. Silver stain detection of proteins; D. Western blot (immunoblot) detection of proteins; Study questions; Exercises III. Oligonucleotide-Directed Mutagenesis; A. Restriction digests of virD2 (in pCS64) and pUC119; B. Purification of DNA fragments from agarose C. Ligation of restriction fragment and vectorD. Transformation of E. coli with the ligated plasmid and recovery of clones; E. Small-scale preparation of plasmid DNA from broth cultures; F. Restriction digest of DNAs: Examination to confirm insert; G. Preparation of single-stranded DNA template; H. Phosphorylation of oligonucleotide; I. Annealing mutant oligonucleotide to template; J. In vitro DNA synthesis by primer extension; K. Transform synthesis reaction into E. coli DH5a; L. Small-scale preparation of plasmid DNA; M. Confirmation of mutants by restriction analysis; Study questions Exercises IV. DNA SequencingA. Polyacrylamide sequencing gel electrophoresis; B. Dideoxy sequencing; C. Automated sequencing; D. Introduction to databases and gene sequence analysis; Study questions; Exercises V. Southern Blot Detection of DNA; A. Preparation of genomic DNA from Agrobacterium tumefaciens; B. Restriction digestion of genomic DNA; C. Agarose gel electrophoresis of restriction fragments; D. Southern blot: Denaturation and blotting of DNA; E. Preparation of probe by nick translation; F. Hybridization and washing of Southern blots; Study questions Exercises VI. Northern Blot Detection of mRNAA. Preparation of RNA from tobacco leaves; B. Agarose-formaldehyde gel electrophoresis; C. Northern blot: Denaturation and blotting of RNA; D. Probe preparation; E. Hybridization and washing of Northern blots; Study questions; Exercises VII. Protein Interaction Analysis in Yeast; A. Yeast transformation; B. Filter ß-galactosidase assay; Index |
| Record Nr. | UNINA-9910827531303321 |
| San Diego, CA, : Academic Press, 1998 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||