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MARC Lista (tabellare)
Molecular analysis and genome discovery [[electronic resource] /] / edited by Ralph Rapley and Stuart Harbron
Molecular analysis and genome discovery [[electronic resource] /] / edited by Ralph Rapley and Stuart Harbron
Edizione [2nd ed.]
Pubbl/distr/stampa Chichester, West Sussex ; ; Hoboken, NJ, : John Wiley & Sons, 2011
Descrizione fisica 1 online resource (317 p.)
Disciplina 615/.7
Altri autori (Persone) RapleyRalph
HarbronStuart
Soggetto topico Molecular diagnosis
Genomics
Proteomics
Pharmacogenomics
Polymerase chain reaction
DNA microarrays
ISBN 1-283-28453-7
9786613284532
1-119-97743-6
1-119-97744-4
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Molecular Analysis and Genome Discovery; Contents; Preface; Contributors; 1 Overview of Genotyping; Introduction; Methods for interrogating SNPs; Commercial platforms for SNP genotyping; Practical recommendations; SNP databases; Methylation analysis; Copy number variation analysis; Second generation sequencing technologies; Conclusions; References; 2 DNA Chip Analysis in Genome Discovery; Introduction; Interrogating a genome; Cross-species hybridization; Comparative genomic hybridization and microarray-based genotyping; Barcodes, DNA microarrays and organism identification; Concluding remarks
References3 qPCR, Theory, Reliability and Use in Molecular Analysis; Sample preparation; RNA quality; Reagents; Assay design; Transparency of published data; Further considerations; Conclusion; References; 4 DNA Analysis in Droplet-Based Microfluidic Devices; Introduction; Continuous-flow microPCR chips; PCR inhibition and carryover contamination; PCR in droplets; Conclusions; References; 5 High-Resolution Melt Profiling; Introduction; Basic concepts of melt profiling; HRMP and polymerase chain reaction; DNA specimens and HRMP; Determining a temperature window for melting
Dyes and platforms for melt profilingScanning PCR products for sequence variation; Genotyping with high-resolution melt profiling; Other applications of HRMP; Final notes; References; 6 Massively Parallel Sequencing; Sanger sequencing; Massively parallel sequencing; Commercially available massively parallel sequencers; Future technologies; Paired-end or mate-paired reads; Target-enrichment strategies for MPS; Applications of MPS; Summary; References; 7 Aptamers for Analysis: Nucleic Acids Ligands in the Post-Genomic Era; Introduction; SELEX; Aptamers in analysis; Imaging with aptamers
Conclusions, outlooks and perspectivesReferences; 8 Use of Nanotechnology for Enhancing of Cancer Biomarker Discovery and Analysis: A Molecular Approach; Introduction; Proteomics and nanotechnology; Nanoscale multicomponent separation; Nanoscale protein detection strategies; Surface-enhanced Raman scattering (SERS); References; 9 Chip-Based Proteomics; Introduction; Lab-on-a-chip; Arrays; Chip-based mass spectrometry; Surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) chip instruments; Microfluidics; Conclusion; References; 10 Antibody Microarrays in Proteome Profiling
IntroductionTechnical aspects; Antibody array applications; Summary; Acknowledgements; References; 11 Biomarker Detection and Molecular Profiling by Multiplex Microbead Suspension Array Based Immunoproteomics; Introduction; Principles of microbead-based multiplexing; Experimental aspects of the multiplex microbead assay; Multiplex microbead assay design and comparison with other methods; Applications of the multiplex microbead assay system for biomedical research and clinical studies; Selected investigational fields for multiplex analysis and examples of applications
Challenges and current limitations
Record Nr. UNINA-9910139595203321
Chichester, West Sussex ; ; Hoboken, NJ, : John Wiley & Sons, 2011
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Molecular analysis and genome discovery [[electronic resource] /] / edited by Ralph Rapley and Stuart Harbron
Molecular analysis and genome discovery [[electronic resource] /] / edited by Ralph Rapley and Stuart Harbron
Edizione [2nd ed.]
Pubbl/distr/stampa Chichester, West Sussex ; ; Hoboken, NJ, : John Wiley & Sons, 2011
Descrizione fisica 1 online resource (317 p.)
Disciplina 615/.7
Altri autori (Persone) RapleyRalph
HarbronStuart
Soggetto topico Molecular diagnosis
Genomics
Proteomics
Pharmacogenomics
Polymerase chain reaction
DNA microarrays
ISBN 1-283-28453-7
9786613284532
1-119-97743-6
1-119-97744-4
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Molecular Analysis and Genome Discovery; Contents; Preface; Contributors; 1 Overview of Genotyping; Introduction; Methods for interrogating SNPs; Commercial platforms for SNP genotyping; Practical recommendations; SNP databases; Methylation analysis; Copy number variation analysis; Second generation sequencing technologies; Conclusions; References; 2 DNA Chip Analysis in Genome Discovery; Introduction; Interrogating a genome; Cross-species hybridization; Comparative genomic hybridization and microarray-based genotyping; Barcodes, DNA microarrays and organism identification; Concluding remarks
References3 qPCR, Theory, Reliability and Use in Molecular Analysis; Sample preparation; RNA quality; Reagents; Assay design; Transparency of published data; Further considerations; Conclusion; References; 4 DNA Analysis in Droplet-Based Microfluidic Devices; Introduction; Continuous-flow microPCR chips; PCR inhibition and carryover contamination; PCR in droplets; Conclusions; References; 5 High-Resolution Melt Profiling; Introduction; Basic concepts of melt profiling; HRMP and polymerase chain reaction; DNA specimens and HRMP; Determining a temperature window for melting
Dyes and platforms for melt profilingScanning PCR products for sequence variation; Genotyping with high-resolution melt profiling; Other applications of HRMP; Final notes; References; 6 Massively Parallel Sequencing; Sanger sequencing; Massively parallel sequencing; Commercially available massively parallel sequencers; Future technologies; Paired-end or mate-paired reads; Target-enrichment strategies for MPS; Applications of MPS; Summary; References; 7 Aptamers for Analysis: Nucleic Acids Ligands in the Post-Genomic Era; Introduction; SELEX; Aptamers in analysis; Imaging with aptamers
Conclusions, outlooks and perspectivesReferences; 8 Use of Nanotechnology for Enhancing of Cancer Biomarker Discovery and Analysis: A Molecular Approach; Introduction; Proteomics and nanotechnology; Nanoscale multicomponent separation; Nanoscale protein detection strategies; Surface-enhanced Raman scattering (SERS); References; 9 Chip-Based Proteomics; Introduction; Lab-on-a-chip; Arrays; Chip-based mass spectrometry; Surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) chip instruments; Microfluidics; Conclusion; References; 10 Antibody Microarrays in Proteome Profiling
IntroductionTechnical aspects; Antibody array applications; Summary; Acknowledgements; References; 11 Biomarker Detection and Molecular Profiling by Multiplex Microbead Suspension Array Based Immunoproteomics; Introduction; Principles of microbead-based multiplexing; Experimental aspects of the multiplex microbead assay; Multiplex microbead assay design and comparison with other methods; Applications of the multiplex microbead assay system for biomedical research and clinical studies; Selected investigational fields for multiplex analysis and examples of applications
Challenges and current limitations
Record Nr. UNINA-9910809498403321
Chichester, West Sussex ; ; Hoboken, NJ, : John Wiley & Sons, 2011
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Molecular and cellular therapeutics [[electronic resource] /] / edited by David B. Whitehouse, Ralph Rapley
Molecular and cellular therapeutics [[electronic resource] /] / edited by David B. Whitehouse, Ralph Rapley
Edizione [2nd ed.]
Pubbl/distr/stampa Chichester, West Sussex, UK, : John Wiley & Sons, 2012
Descrizione fisica 1 online resource (331 p.)
Disciplina 572.8
Altri autori (Persone) WhitehouseDavid <1946->
RapleyRalph
Soggetto topico Molecular biology
Cellular therapy
ISBN 1-119-96780-5
1-280-58738-5
9786613617217
1-119-96730-9
1-119-96729-5
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Molecular and CellularTherapeutics; Contents; List of contributors; Preface; 1 Cytochrome P450 pharmacogenetics: from bench to bedside; 2 Cancer biomarkers for diagnosis, prognosis and therapy; 3 HER2 targeted therapy-induced gastrointestinal toxicity: from the clinical experience to possible molecular mechanisms; 4 Antibody-targeted photodynamic therapy; 5 Anti-ageing strategy of the lung for chronic inflammatory respiratory disease - targeting protein deacetylases; 6 RNA interference: from basics to therapeutics; 7 Delivery of RNAi effectors by tkRNAi; 8 Human stem cell therapy
9 Gene therapy in organ transplantation10 Advances in the treatment of Alzheimer's disease; 11 Novel molecular therapeutics in Parkinson's disease; 12 Emerging insights and therapies for human microbial disease; 13 Vaccine design and vaccination; Index
Record Nr. UNINA-9910141346603321
Chichester, West Sussex, UK, : John Wiley & Sons, 2012
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Molecular biology and biotechnology [[electronic resource]]
Molecular biology and biotechnology [[electronic resource]]
Edizione [4th ed. /]
Pubbl/distr/stampa Cambridge, : Royal Society of Chemistry, 2000
Descrizione fisica 1 online resource (589 p.)
Disciplina 574.88
Altri autori (Persone) WalkerJohn M. <1948->
RapleyRalph
Soggetto topico Molecular biology
Biotechnology
Soggetto genere / forma Electronic books.
ISBN 1-84755-149-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Ch. 1. Fermentation technology -- Ch. 2. Molecular analysis and application techniques -- Ch. 3. Recombinanat DNA technology -- Ch. 4. The expression of foreign DNA in bacteria -- Ch. 5. Yeast cloning and biotechnology -- Ch. 6. Cloning genes in mammalian cell-lines -- Ch. 7. Plant biotechnology -- Ch. 8. Molecular, structural and chemical biology in pharmaceutical research -- Ch. 9. Genetically modified foods -- Ch. 10. Molecular diagnosis of inherited disease -- Ch. 11. DNA in forensic science -- Ch. 12. Vaccination and gene manipulation -- Ch. 13. Transgenesis -- Ch. 14. Protein engineering -- Ch. 15. Bioinformatics -- Ch. 16. Immobilization of biocatalysts -- Ch. 17. Downstream processing: protein extraction and purification -- Ch. 18. Monoclonal antibodies -- Ch. 19. Biosensors.
Record Nr. UNINA-9910456705403321
Cambridge, : Royal Society of Chemistry, 2000
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Molecular biology and biotechnology [[electronic resource]]
Molecular biology and biotechnology [[electronic resource]]
Edizione [4th ed. /]
Pubbl/distr/stampa Cambridge, : Royal Society of Chemistry, 2000
Descrizione fisica 1 online resource (589 p.)
Disciplina 574.88
Altri autori (Persone) WalkerJohn M. <1948->
RapleyRalph
Soggetto topico Molecular biology
Biotechnology
ISBN 1-84755-149-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Ch. 1. Fermentation technology -- Ch. 2. Molecular analysis and application techniques -- Ch. 3. Recombinanat DNA technology -- Ch. 4. The expression of foreign DNA in bacteria -- Ch. 5. Yeast cloning and biotechnology -- Ch. 6. Cloning genes in mammalian cell-lines -- Ch. 7. Plant biotechnology -- Ch. 8. Molecular, structural and chemical biology in pharmaceutical research -- Ch. 9. Genetically modified foods -- Ch. 10. Molecular diagnosis of inherited disease -- Ch. 11. DNA in forensic science -- Ch. 12. Vaccination and gene manipulation -- Ch. 13. Transgenesis -- Ch. 14. Protein engineering -- Ch. 15. Bioinformatics -- Ch. 16. Immobilization of biocatalysts -- Ch. 17. Downstream processing: protein extraction and purification -- Ch. 18. Monoclonal antibodies -- Ch. 19. Biosensors.
Record Nr. UNINA-9910780937403321
Cambridge, : Royal Society of Chemistry, 2000
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Molecular biology and biotechnology [[electronic resource]]
Molecular biology and biotechnology [[electronic resource]]
Edizione [4th ed. /]
Pubbl/distr/stampa Cambridge, : Royal Society of Chemistry, 2000
Descrizione fisica 1 online resource (589 p.)
Disciplina 574.88
Altri autori (Persone) WalkerJohn M. <1948->
RapleyRalph
Soggetto topico Molecular biology
Biotechnology
ISBN 1-84755-149-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Ch. 1. Fermentation technology -- Ch. 2. Molecular analysis and application techniques -- Ch. 3. Recombinanat DNA technology -- Ch. 4. The expression of foreign DNA in bacteria -- Ch. 5. Yeast cloning and biotechnology -- Ch. 6. Cloning genes in mammalian cell-lines -- Ch. 7. Plant biotechnology -- Ch. 8. Molecular, structural and chemical biology in pharmaceutical research -- Ch. 9. Genetically modified foods -- Ch. 10. Molecular diagnosis of inherited disease -- Ch. 11. DNA in forensic science -- Ch. 12. Vaccination and gene manipulation -- Ch. 13. Transgenesis -- Ch. 14. Protein engineering -- Ch. 15. Bioinformatics -- Ch. 16. Immobilization of biocatalysts -- Ch. 17. Downstream processing: protein extraction and purification -- Ch. 18. Monoclonal antibodies -- Ch. 19. Biosensors.
Record Nr. UNINA-9910824019703321
Cambridge, : Royal Society of Chemistry, 2000
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Route maps in gene technology [[electronic resource] /] / Matthew R. Walker, with Ralph Rapley
Route maps in gene technology [[electronic resource] /] / Matthew R. Walker, with Ralph Rapley
Autore Walker Matthew R
Pubbl/distr/stampa Oxford ; ; Malden, MA, : Blackwell Science, 1997
Descrizione fisica 1 online resource (336 p.)
Disciplina 660.65
660/.65
Altri autori (Persone) RapleyRalph
Soggetto topico Genetic engineering
Human genetics
ISBN 1-282-37168-1
9786612371684
1-4443-1361-4
1-4443-1360-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto ROUTE MAPS IN GENE TECHNOLOGY; CONTENTS; PREFACE; ABOUT THE ROUTE MAPS FORMAT; 1. THE CONCEPT OF GENES IS DEVELOPED; 2. GENES ARE LOCATED TO CHROMOSOMES; 3. GENES ARE COMPOSED OF DNA; 4. THE CHEMICAL BUILDING BLOCKS OF NUCLEIC ACIDS; 5. FORMATION OF THE DNA DOUBLE HELIX; 6. PACKAGING OF DNA WITHIN CELLS; 7. CHROMATIN STRUCTURE AND THE FUNCTIONAL ACTIVITY OF GENES; 8. TYPES AND FUNCTIONS OF DNA-PROTEIN INTERACTIONS; 9. ORGANISATION OF GENOMES INTO MULTIPLE CHROMOSOMES; 10. DISTRIBUTION OF NUCLEIC ACIDS WITHIN EUKARYOTIC CELLS; 11. TYPES OF RNA MOLECULES
12. THE ANATOMY OF EUKARYOTIC CHROMOSOMES13. ORGANISATION OF GENES WITHIN CHROMOSOMES; 14. THE MOLECULAR ANATOMY OF EUKARYOTIC GENES; 15. CHROMOSOME ABERRATIONS AND LINKS TO HUMAN DISEASE; 16. TYPES OF MUTATIONS AND THEIR EFFECTS; 17. FORMS OF CHEMICALLY ALTERED DNA; 18. DNA REPAIR MECHANISMS; 19. LINKAGE ANALYSIS; 20. PEDIGREE ANALYSIS AND MODES OF INHERITANCE; 21. GENES DICTATE THE NATURE OF PROTEINS; 22. THE NATURE OF THE GENETIC CODE; 23. TRANSCRIPTION: FORMING GENETIC MESSAGES; 24. POST-TRANSCRIPTIONAL PROCESSING OF MESSENGER RNA; 25. TRANSFER AND RIBOSOMAL RNA PROCESSING/MODIFICATION
26. MECHANISMS REGULATING GENE EXPRESSION27. TRANSCRIPTIONAL REGULATORY SEQUENCES; 28. OPERONS AND PROKARYOTIC CONTROL OF GENE EXPRESSION; 29. TRANSCRIPTION FACTORS AND GENE EXPRESSION; 30. IN VIVO TRANSLATION: DECODING GENETIC MESSAGES; 31. SEQUENCES INVOLVED IN CELLULAR PROTEIN TARGETING; 32. EUKARYOTIC CELL DIVISION: MITOSIS AND MEIOSIS; 33. MOLECULAR MECHANISMS OF CELL CYCLE CONTR0L; 34. GENETIC RECOMBINATION MECHANISMS; 35. GENE TRANSFER DURING BACTERIAL REPRODUCTION; 36. TRANSPOSABLE GENETIC ELEMENTS: TRANSPOSONS; 37. IN VIVO DNA REPLICATION; 38. GENETIC CONTROL OF DEVELOPMENT
39. THE NATURAL BIOLOGY OF BACTERIOPHAGES40. BACTERIOPHAGE GENETICS; 41. RECOMBINANT DNA TECHNOLOGY; 42. ENZYMES COMMONLY USED IN MOLECULAR BIOLOGY METHODS; 43. RESTRICTION ENDONUCLEASES; 44. RESTRICTION FRAGMENT LENGTH POLYMORPHISMS; 45. ISOLATION OF NUCLEIC ACIDS FROM CELLS AND TISSUES; 46. VISUALISING NUCLEIC ACIDS; 47. ELECTROPHORESIS OF NUCLEIC ACIDS; 48. IN VITRO HYBRIDISATION; 49. TYPES OF HYBRIDISATION ASSAY FORMATS; 50. SOUTHERN BLOTTING; 51. IN SITU HYBRIDISATION; 52. MEASURING TRANSCRIPTIONAL ACTIVITY VIA MESSENGER RNA; 53. CONVERTING MESSENGER RNA INTO COMPLEMENTARY DNA
54. METHODS FOR DETERMINING DNA NUCLEOTIDE SEQUENCES55. THE POLYMERASE CHAIN REACTION; 56. ALTERNATIVES TO PCR-BASED IN VITRO DNA/RNA AMPLIFICATION; 57. IN VITRO TRANSLATION METHODS; 58. TYPES AND METHODS OF GENE PROBE GENERATION; 59. CHEMICAL SYNTHESIS OF OLIGONUCLEOTIDES; 60. TYPES AND APPLICATIONS OF NUCLEOTIDE ANALOGUES; 61. METHODS FOR LABELLING GENE PROBES; 62. FUNDAMENTAL PRINCIPLES OF CLONING; 63. THE NATURE OF CLONING VECTORS; 64. INSERTING FOREIGN DNA INTO VECTORS; 65. THE DEVELOPMENT OF BACTERIOPHAGE VECTORS; 66. PLASMIDS: DEVELOPMENT AS CLONING VECTORS
67. YEAST-DERIVED PLASMID VECTORS
Record Nr. UNISA-996200052303316
Walker Matthew R  
Oxford ; ; Malden, MA, : Blackwell Science, 1997
Materiale a stampa
Lo trovi qui: Univ. di Salerno
Opac: Controlla la disponibilità qui
Route maps in gene technology [[electronic resource] /] / Matthew R. Walker, with Ralph Rapley
Route maps in gene technology [[electronic resource] /] / Matthew R. Walker, with Ralph Rapley
Autore Walker Matthew R
Pubbl/distr/stampa Oxford ; ; Malden, MA, : Blackwell Science, 1997
Descrizione fisica 1 online resource (336 p.)
Disciplina 660.65
660/.65
Altri autori (Persone) RapleyRalph
Soggetto topico Genetic engineering
Human genetics
ISBN 1-282-37168-1
9786612371684
1-4443-1361-4
1-4443-1360-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto ROUTE MAPS IN GENE TECHNOLOGY; CONTENTS; PREFACE; ABOUT THE ROUTE MAPS FORMAT; 1. THE CONCEPT OF GENES IS DEVELOPED; 2. GENES ARE LOCATED TO CHROMOSOMES; 3. GENES ARE COMPOSED OF DNA; 4. THE CHEMICAL BUILDING BLOCKS OF NUCLEIC ACIDS; 5. FORMATION OF THE DNA DOUBLE HELIX; 6. PACKAGING OF DNA WITHIN CELLS; 7. CHROMATIN STRUCTURE AND THE FUNCTIONAL ACTIVITY OF GENES; 8. TYPES AND FUNCTIONS OF DNA-PROTEIN INTERACTIONS; 9. ORGANISATION OF GENOMES INTO MULTIPLE CHROMOSOMES; 10. DISTRIBUTION OF NUCLEIC ACIDS WITHIN EUKARYOTIC CELLS; 11. TYPES OF RNA MOLECULES
12. THE ANATOMY OF EUKARYOTIC CHROMOSOMES13. ORGANISATION OF GENES WITHIN CHROMOSOMES; 14. THE MOLECULAR ANATOMY OF EUKARYOTIC GENES; 15. CHROMOSOME ABERRATIONS AND LINKS TO HUMAN DISEASE; 16. TYPES OF MUTATIONS AND THEIR EFFECTS; 17. FORMS OF CHEMICALLY ALTERED DNA; 18. DNA REPAIR MECHANISMS; 19. LINKAGE ANALYSIS; 20. PEDIGREE ANALYSIS AND MODES OF INHERITANCE; 21. GENES DICTATE THE NATURE OF PROTEINS; 22. THE NATURE OF THE GENETIC CODE; 23. TRANSCRIPTION: FORMING GENETIC MESSAGES; 24. POST-TRANSCRIPTIONAL PROCESSING OF MESSENGER RNA; 25. TRANSFER AND RIBOSOMAL RNA PROCESSING/MODIFICATION
26. MECHANISMS REGULATING GENE EXPRESSION27. TRANSCRIPTIONAL REGULATORY SEQUENCES; 28. OPERONS AND PROKARYOTIC CONTROL OF GENE EXPRESSION; 29. TRANSCRIPTION FACTORS AND GENE EXPRESSION; 30. IN VIVO TRANSLATION: DECODING GENETIC MESSAGES; 31. SEQUENCES INVOLVED IN CELLULAR PROTEIN TARGETING; 32. EUKARYOTIC CELL DIVISION: MITOSIS AND MEIOSIS; 33. MOLECULAR MECHANISMS OF CELL CYCLE CONTR0L; 34. GENETIC RECOMBINATION MECHANISMS; 35. GENE TRANSFER DURING BACTERIAL REPRODUCTION; 36. TRANSPOSABLE GENETIC ELEMENTS: TRANSPOSONS; 37. IN VIVO DNA REPLICATION; 38. GENETIC CONTROL OF DEVELOPMENT
39. THE NATURAL BIOLOGY OF BACTERIOPHAGES40. BACTERIOPHAGE GENETICS; 41. RECOMBINANT DNA TECHNOLOGY; 42. ENZYMES COMMONLY USED IN MOLECULAR BIOLOGY METHODS; 43. RESTRICTION ENDONUCLEASES; 44. RESTRICTION FRAGMENT LENGTH POLYMORPHISMS; 45. ISOLATION OF NUCLEIC ACIDS FROM CELLS AND TISSUES; 46. VISUALISING NUCLEIC ACIDS; 47. ELECTROPHORESIS OF NUCLEIC ACIDS; 48. IN VITRO HYBRIDISATION; 49. TYPES OF HYBRIDISATION ASSAY FORMATS; 50. SOUTHERN BLOTTING; 51. IN SITU HYBRIDISATION; 52. MEASURING TRANSCRIPTIONAL ACTIVITY VIA MESSENGER RNA; 53. CONVERTING MESSENGER RNA INTO COMPLEMENTARY DNA
54. METHODS FOR DETERMINING DNA NUCLEOTIDE SEQUENCES55. THE POLYMERASE CHAIN REACTION; 56. ALTERNATIVES TO PCR-BASED IN VITRO DNA/RNA AMPLIFICATION; 57. IN VITRO TRANSLATION METHODS; 58. TYPES AND METHODS OF GENE PROBE GENERATION; 59. CHEMICAL SYNTHESIS OF OLIGONUCLEOTIDES; 60. TYPES AND APPLICATIONS OF NUCLEOTIDE ANALOGUES; 61. METHODS FOR LABELLING GENE PROBES; 62. FUNDAMENTAL PRINCIPLES OF CLONING; 63. THE NATURE OF CLONING VECTORS; 64. INSERTING FOREIGN DNA INTO VECTORS; 65. THE DEVELOPMENT OF BACTERIOPHAGE VECTORS; 66. PLASMIDS: DEVELOPMENT AS CLONING VECTORS
67. YEAST-DERIVED PLASMID VECTORS
Record Nr. UNINA-9910139786403321
Walker Matthew R  
Oxford ; ; Malden, MA, : Blackwell Science, 1997
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Route maps in gene technology [[electronic resource] /] / Matthew R. Walker, with Ralph Rapley
Route maps in gene technology [[electronic resource] /] / Matthew R. Walker, with Ralph Rapley
Autore Walker Matthew R
Pubbl/distr/stampa Oxford ; ; Malden, MA, : Blackwell Science, 1997
Descrizione fisica 1 online resource (336 p.)
Disciplina 660.65
660/.65
Altri autori (Persone) RapleyRalph
Soggetto topico Genetic engineering
Human genetics
ISBN 1-282-37168-1
9786612371684
1-4443-1361-4
1-4443-1360-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto ROUTE MAPS IN GENE TECHNOLOGY; CONTENTS; PREFACE; ABOUT THE ROUTE MAPS FORMAT; 1. THE CONCEPT OF GENES IS DEVELOPED; 2. GENES ARE LOCATED TO CHROMOSOMES; 3. GENES ARE COMPOSED OF DNA; 4. THE CHEMICAL BUILDING BLOCKS OF NUCLEIC ACIDS; 5. FORMATION OF THE DNA DOUBLE HELIX; 6. PACKAGING OF DNA WITHIN CELLS; 7. CHROMATIN STRUCTURE AND THE FUNCTIONAL ACTIVITY OF GENES; 8. TYPES AND FUNCTIONS OF DNA-PROTEIN INTERACTIONS; 9. ORGANISATION OF GENOMES INTO MULTIPLE CHROMOSOMES; 10. DISTRIBUTION OF NUCLEIC ACIDS WITHIN EUKARYOTIC CELLS; 11. TYPES OF RNA MOLECULES
12. THE ANATOMY OF EUKARYOTIC CHROMOSOMES13. ORGANISATION OF GENES WITHIN CHROMOSOMES; 14. THE MOLECULAR ANATOMY OF EUKARYOTIC GENES; 15. CHROMOSOME ABERRATIONS AND LINKS TO HUMAN DISEASE; 16. TYPES OF MUTATIONS AND THEIR EFFECTS; 17. FORMS OF CHEMICALLY ALTERED DNA; 18. DNA REPAIR MECHANISMS; 19. LINKAGE ANALYSIS; 20. PEDIGREE ANALYSIS AND MODES OF INHERITANCE; 21. GENES DICTATE THE NATURE OF PROTEINS; 22. THE NATURE OF THE GENETIC CODE; 23. TRANSCRIPTION: FORMING GENETIC MESSAGES; 24. POST-TRANSCRIPTIONAL PROCESSING OF MESSENGER RNA; 25. TRANSFER AND RIBOSOMAL RNA PROCESSING/MODIFICATION
26. MECHANISMS REGULATING GENE EXPRESSION27. TRANSCRIPTIONAL REGULATORY SEQUENCES; 28. OPERONS AND PROKARYOTIC CONTROL OF GENE EXPRESSION; 29. TRANSCRIPTION FACTORS AND GENE EXPRESSION; 30. IN VIVO TRANSLATION: DECODING GENETIC MESSAGES; 31. SEQUENCES INVOLVED IN CELLULAR PROTEIN TARGETING; 32. EUKARYOTIC CELL DIVISION: MITOSIS AND MEIOSIS; 33. MOLECULAR MECHANISMS OF CELL CYCLE CONTR0L; 34. GENETIC RECOMBINATION MECHANISMS; 35. GENE TRANSFER DURING BACTERIAL REPRODUCTION; 36. TRANSPOSABLE GENETIC ELEMENTS: TRANSPOSONS; 37. IN VIVO DNA REPLICATION; 38. GENETIC CONTROL OF DEVELOPMENT
39. THE NATURAL BIOLOGY OF BACTERIOPHAGES40. BACTERIOPHAGE GENETICS; 41. RECOMBINANT DNA TECHNOLOGY; 42. ENZYMES COMMONLY USED IN MOLECULAR BIOLOGY METHODS; 43. RESTRICTION ENDONUCLEASES; 44. RESTRICTION FRAGMENT LENGTH POLYMORPHISMS; 45. ISOLATION OF NUCLEIC ACIDS FROM CELLS AND TISSUES; 46. VISUALISING NUCLEIC ACIDS; 47. ELECTROPHORESIS OF NUCLEIC ACIDS; 48. IN VITRO HYBRIDISATION; 49. TYPES OF HYBRIDISATION ASSAY FORMATS; 50. SOUTHERN BLOTTING; 51. IN SITU HYBRIDISATION; 52. MEASURING TRANSCRIPTIONAL ACTIVITY VIA MESSENGER RNA; 53. CONVERTING MESSENGER RNA INTO COMPLEMENTARY DNA
54. METHODS FOR DETERMINING DNA NUCLEOTIDE SEQUENCES55. THE POLYMERASE CHAIN REACTION; 56. ALTERNATIVES TO PCR-BASED IN VITRO DNA/RNA AMPLIFICATION; 57. IN VITRO TRANSLATION METHODS; 58. TYPES AND METHODS OF GENE PROBE GENERATION; 59. CHEMICAL SYNTHESIS OF OLIGONUCLEOTIDES; 60. TYPES AND APPLICATIONS OF NUCLEOTIDE ANALOGUES; 61. METHODS FOR LABELLING GENE PROBES; 62. FUNDAMENTAL PRINCIPLES OF CLONING; 63. THE NATURE OF CLONING VECTORS; 64. INSERTING FOREIGN DNA INTO VECTORS; 65. THE DEVELOPMENT OF BACTERIOPHAGE VECTORS; 66. PLASMIDS: DEVELOPMENT AS CLONING VECTORS
67. YEAST-DERIVED PLASMID VECTORS
Record Nr. UNINA-9910813412303321
Walker Matthew R  
Oxford ; ; Malden, MA, : Blackwell Science, 1997
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui