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Flow cytometry of hematological malignancies / / Claudio Ortolani
Flow cytometry of hematological malignancies / / Claudio Ortolani
Autore Ortolani C (Claudio)
Edizione [Second edition.]
Pubbl/distr/stampa Hoboken, New Jersey ; ; Chichester, West Sussex, England : , : Wiley Blackwell, , [2021]
Descrizione fisica 1 online resource (467 pages)
Disciplina 574.87028
Soggetto topico Flow cytometry
Hematological oncology
Flow cytometry - Methodology
Soggetto genere / forma Electronic books.
ISBN 1-119-61130-X
1-119-61127-X
1-119-61128-8
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Cover -- Title Page -- Copyright Page -- Contents -- Foreword to the Second Edition -- Foreword to the First Edition -- Foreword to the First Edition -- Preface to the Second Edition -- Preface to the First Edition -- Abbreviations -- Chapter 1 Antigens -- CD1 Antigens -- CD2 Antigen -- CD3 Antigen -- CD4 Antigen -- CD5 Antigen -- CD7 Antigen -- CD8 Antigen -- CD10 Antigen -- CD11b Antigen -- CD11c Antigen -- CD13 Antigen -- CD14 Antigen -- CD15 Antigen -- CD16 Antigen -- CD19 Antigen -- CD20 Antigen -- CD22 Antigen -- CD23 Antigen -- CD24 Antigen -- CD25 Antigen -- CD26 Antigen -- CD27 Antigen -- CD28 Antigen -- CD30 Antigen -- CD33 Antigen -- CD34 Antigen -- CD38 Antigen -- CD43 Antigen -- CD45 Antigen -- CD45 Isoforms -- CD49 Antigens -- CD56 Antigen -- CD57 Antigen -- CD61 Antigen -- CD62L Antigen -- CD64 Antigen -- CD65 Antigen -- CD66c Antigen -- CD71 Antigen -- CD79 Antigen -- CD81 Antigen -- CD103 Antigen -- CD117 Antigen -- CD123 Antigen -- CD138 Antigen -- CD200 Antigen -- CD305 Antigen -- CD307 (IRTA) Family -- CD371 Antigen -- BCL-2 Protein -- Chemokines and Chemokine Receptors -- Chemokines -- Chemokine Receptors -- CRLF2 Antigen -- Cytotoxic Proteins -- HLA-DR Antigen -- Immunoglobulins -- KIRs, CD158 Isoforms -- Myeloperoxidase -- NG2 Antigen -- PCA-1 Antigen -- ROR1 Antigen -- SLAM Molecules and SLAM-Associated Protein -- SOX11 -- T-Cell Receptor (TCR) -- Terminal Deoxy-Nucleotidyl Transferase -- Toll-Like Receptors -- VS38 Antigen -- ZAP-70 Protein -- Chapter 2 Diseases -- Myeloproliferative neoplasms -- Chronic myeloid leukemia (CML) -- Myeloproliferative neoplasms other than CML -- Chronic neutrophilic leukemia (CNL) -- Polycythemia vera (PV) -- Primary myelofibrosis (PMF) -- Essential thrombocythemia (ET) -- Chronic eosinophilic leukemia (CEL) -- Mastocytosis -- Acute mast-cell leukemia (AMCL).
Chronic mast-cell leukemia (CMCL) -- Myelomastocytic leukemia (MML) -- Myelodysplastic/myeloproliferative neoplasms -- Chronic myelomonocytic leukemia (CMML) -- Other myelodysplastic/myeloproliferative neoplasms and related conditions -- Juvenile myelomonocytic leukemia (JMML) -- Atypical CML bcr/abl negative (ACML) -- RAS-associated autoimmune leukoproliferative disorder (RALD) -- Myelodysplastic syndromes -- Myeloid neoplasms with germline predisposition -- Acute myeloid leukemias -- AMLs with recurrent genetic anomalies -- AMLs with chromosomal anomalies -- AMLs with gene mutations -- Relationships between genotype and phenotype in cases of AML not recognized as separate entities in WHO 2017 -- AMLs with myelodysplasia-related changes (AML-MRC) -- AMLs not otherwise specified -- AML with minimal differentiation -- AML without maturation -- AML with maturation -- Acute myelomonocytic leukemia (AMMoL) -- Acute monoblastic and monocytic leukemia (AMoL) -- Pure erythroid leukemia (PEL) -- Acute megakaryoblastic leukemia (AMKL) -- Acute basophilic leukemia (ABL) -- Myeloid proliferations associated with Down syndrome -- Transient abnormal myelopoiesis (TAM) -- AMLs in patients with Down syndrome -- Blastic plasmacytoid dendritic cell neoplasm (BPDCN/PDCL) -- Acute leukemias with ambiguous lineage attribution (ALAL) -- Acute undifferentiated leukemias (AUL) -- Mixed phenotype acute leukemias (MPAL) -- Neoplastic diseases of B and T lymphatic precursors -- B lymphoblastic leukemia/lymphoma, not otherwise specified (B-ALL/LBLnos) -- B lymphoblastic leukemia/lymphoma with recurrent genetic anomalies -- Relationships between genotype and phenotype in cases of B-ALL not recognized as separate entities in WHO 2017 -- T lymphoblastic leukemia/lymphoma (T-ALL/LBL) -- Early T-cell precursor lymphoblastic leukemia (ETP-ALL).
NK lymphoblastic leukemia/lymphoma (NK-ALL/LBL) -- Neoplastic diseases of mature B cells -- Chronic lymphocytic leukemia/small lymphocytic lymphoma (B-CLL/SLL) -- Familial B-CLL -- Richter syndrome -- Monoclonal B-cell lymphocytosis (MBL) -- CLL-like monoclonal B lymphocytosis -- Non-CLL-like monoclonal B lymphocytosis -- B-cell prolymphocytic leukemia (B-PLL) -- Lymphoplasmacytic lymphoma (LPL) -- Heavy chain disease (HCD) -- μ heavy chain disease -- α heavy chain disease -- Hairy cell leukemia (HCL) -- Hairy cell leukemia, variant (HCL-v) -- Hairy cell leukemia, Japanese variant (HCL-J) -- Splenic diffuse red pulp lymphoma (SDRPL) -- Marginal zone lymphomas (MZL) -- Nodal marginal zone lymphoma (NMZL) -- Splenic marginal zone lymphoma (SMZL) -- Extranodal marginal zone lymphoma (EMZL/MALToma) -- Clonal B-cell lymphocytosis with MZL-like phenotype (CBL-MZ) -- Follicular lymphoma (FCL) -- Testicular follicular lymphoma -- Duodenal type follicular lymphoma -- Pediatric type follicular lymphoma -- Primitive cutaneous follicular lymphoma (PCFL) -- Large B-cell lymphoma with IRF4 rearrangement -- Mantle-cell lymphoma (MCL) -- Blastic mantle-cell lymphoma (BMCL) -- Leukemic non nodal mantle-cell lymphoma -- DLBCL not otherwise specified (DLBCLnos) -- CD5(+) diffuse large cell lymphoma (CD5(+) DLBCL) -- T-cell/histiocyte-rich B-cell lymphoma (THRLBCL) -- Primary DLBCL of the CNS (PCNSL) -- Primary cutaneous DLBCL, "leg type -- EBV(+) DLBCLnos -- DLBCL associated with chronic inflammation (PAL) -- Fibrin associated DLBCL -- Lymphomatoid granulomatosis (LYG) -- Primary mediastinal B-cell lymphoma (PMBCL) -- Intravascular large B-cell lymphoma (IVBCL) -- ALK-positive large cell lymphoma (ALK(+) LBCL) -- Plasmablastic lymphoma (PBL) -- Primary effusion lymphoma (PEL) -- HHV8-associated lymphoproliferative disorders -- HHV8-positive DLBCL.
HHV8-positive germinotropic lymphoproliferative disorder -- Burkitt lymphoma (BL) -- Burkitt leukemia with immature phenotype -- Burkitt-like lymphoma with 11q aberrations -- High-grade B-cell lymphoma (HGBL) -- Plasma cell neoplasms -- Monoclonal gammopathies of undetermined significance (MGUS) -- Multiple myeloma (MM) -- Plasma cell leukemia (PCL) -- Neoplastic diseases of mature T and NK cells -- T-cell prolymphocytic leukemia (T-PLL) -- T-cell large granular lymphocytic leukemia (T-LGL) -- Chronic lymphoproliferative disorders of NK cells (CLPD-NK/CNKL) -- Aggressive NK-cell leukemia (ANKL) -- Adult T-cell leukemia/lymphoma (ATLL) -- Extranodal NK/T-cell lymphoma, "nasal type" (ENKTL) -- Intestinal T-cell lymphomas (ITCL) -- Enteropathy-associated T-cell lymphoma (EATCL) -- Monomorphic epitheliotropic intestinal T-cell lymphoma (MEITL) -- Indolent gastro-intestinal T lymphoproliferative disorder (indolent GI T-LPD) -- Hepatosplenic T-cell lymphoma (HSTCL) -- Subcutaneous panniculitis-like T-cell lymphoma (SPTCL) -- Mycosis fungoides (MF) -- Sezary syndrome (SS) -- Primary cutaneous CD30(+) lymphoproliferative disorders -- Lymphomatoid papulosis (LyP) -- Primary cutaneous anaplastic T-cell lymphoma (pcALCL) -- Primary cutaneous peripheral T-cell lymphoma (PTCL) -- Primary cutaneous TCRƒÁƒÂ(+) T-cell lymphoma (PCGD-TCL) -- Primary cutaneous CD8(+) aggressive epidermotropic cytotoxic T-cell lymphoma (PCAETL) -- Primary cutaneous acral CD8(+) T-cell lymphoma (PCATCL) -- Primary cutaneous lymphoma of the medium/small CD4(+) T cells (PCSM-TCL) -- Peripheral T-cell lymphoma, not otherwise specified (PTCLnos) -- Nodal lymphomas of follicular T-helper derivation -- Angioimmunoblastic T-cell lymphoma (AITL) -- Follicular T-cell lymphoma (FTCL) -- Nodal PTCL with follicular T-helper phenotype -- Anaplastic large cell lymphoma ALK(+) (ALCL ALK(+)).
Anaplastic large cell lymphoma ALK(-) (ALCL ALK(-)) -- Breast implant.associated anaplastic large cell lymphoma (biaALCL) -- Hodgkin lymphomas -- Classic Hodgkin lymphoma (CHL) -- Nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) -- Neoplastic diseases of histiocytic and dendritic cells -- Histiocytic sarcoma (HS) -- Langerhans cell histiocytosis (LCH) -- Indeterminate dendritic cell tumor (IDCT) -- Interdigitating dendritic cell sarcoma (IDCS) -- Follicular dendritic cell sarcoma (FDCS) -- Erdheim.Chester disease (EDC) -- Chapter 3 Appendix -- Acute leukemias not recognized by the 2017 WHO classification -- Acute leukemia of myeloid/NK precursors (M/NK-AL) -- Acute leukemia of myeloid dendritic cells (MDCL) -- Acute leukemia of Langerhans cells -- Composite lymphomas -- Hypereosinophilic syndrome (HES), lymphocyte variant -- Indolent T lymphoblastic proliferations (iT]LBP) -- Polyclonal lymphocytoses of B lymphocytes -- Persistent polyclonal B]cell lymphocytosis (PPBL) -- Persistent polyclonal CD5(+) B]cell lymphocytosis -- Persistent polyclonal B]cell lymphocytosis, Japanese (hairy) variant -- Polyclonal plasmacytoses -- Small round (blue) cell tumors (SR(B)CT) -- References -- Index -- EULA.
Record Nr. UNINA-9910555117403321
Ortolani C (Claudio)  
Hoboken, New Jersey ; ; Chichester, West Sussex, England : , : Wiley Blackwell, , [2021]
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Flow cytometry of hematological malignancies / / Claudio Ortolani
Flow cytometry of hematological malignancies / / Claudio Ortolani
Autore Ortolani C (Claudio)
Edizione [Second edition.]
Pubbl/distr/stampa Hoboken, New Jersey ; ; Chichester, West Sussex, England : , : Wiley Blackwell, , [2021]
Descrizione fisica 1 online resource (467 pages)
Disciplina 574.87028
Soggetto topico Flow cytometry
Hematological oncology
Flow cytometry - Methodology
ISBN 1-119-61130-X
1-119-61127-X
1-119-61128-8
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Cover -- Title Page -- Copyright Page -- Contents -- Foreword to the Second Edition -- Foreword to the First Edition -- Foreword to the First Edition -- Preface to the Second Edition -- Preface to the First Edition -- Abbreviations -- Chapter 1 Antigens -- CD1 Antigens -- CD2 Antigen -- CD3 Antigen -- CD4 Antigen -- CD5 Antigen -- CD7 Antigen -- CD8 Antigen -- CD10 Antigen -- CD11b Antigen -- CD11c Antigen -- CD13 Antigen -- CD14 Antigen -- CD15 Antigen -- CD16 Antigen -- CD19 Antigen -- CD20 Antigen -- CD22 Antigen -- CD23 Antigen -- CD24 Antigen -- CD25 Antigen -- CD26 Antigen -- CD27 Antigen -- CD28 Antigen -- CD30 Antigen -- CD33 Antigen -- CD34 Antigen -- CD38 Antigen -- CD43 Antigen -- CD45 Antigen -- CD45 Isoforms -- CD49 Antigens -- CD56 Antigen -- CD57 Antigen -- CD61 Antigen -- CD62L Antigen -- CD64 Antigen -- CD65 Antigen -- CD66c Antigen -- CD71 Antigen -- CD79 Antigen -- CD81 Antigen -- CD103 Antigen -- CD117 Antigen -- CD123 Antigen -- CD138 Antigen -- CD200 Antigen -- CD305 Antigen -- CD307 (IRTA) Family -- CD371 Antigen -- BCL-2 Protein -- Chemokines and Chemokine Receptors -- Chemokines -- Chemokine Receptors -- CRLF2 Antigen -- Cytotoxic Proteins -- HLA-DR Antigen -- Immunoglobulins -- KIRs, CD158 Isoforms -- Myeloperoxidase -- NG2 Antigen -- PCA-1 Antigen -- ROR1 Antigen -- SLAM Molecules and SLAM-Associated Protein -- SOX11 -- T-Cell Receptor (TCR) -- Terminal Deoxy-Nucleotidyl Transferase -- Toll-Like Receptors -- VS38 Antigen -- ZAP-70 Protein -- Chapter 2 Diseases -- Myeloproliferative neoplasms -- Chronic myeloid leukemia (CML) -- Myeloproliferative neoplasms other than CML -- Chronic neutrophilic leukemia (CNL) -- Polycythemia vera (PV) -- Primary myelofibrosis (PMF) -- Essential thrombocythemia (ET) -- Chronic eosinophilic leukemia (CEL) -- Mastocytosis -- Acute mast-cell leukemia (AMCL).
Chronic mast-cell leukemia (CMCL) -- Myelomastocytic leukemia (MML) -- Myelodysplastic/myeloproliferative neoplasms -- Chronic myelomonocytic leukemia (CMML) -- Other myelodysplastic/myeloproliferative neoplasms and related conditions -- Juvenile myelomonocytic leukemia (JMML) -- Atypical CML bcr/abl negative (ACML) -- RAS-associated autoimmune leukoproliferative disorder (RALD) -- Myelodysplastic syndromes -- Myeloid neoplasms with germline predisposition -- Acute myeloid leukemias -- AMLs with recurrent genetic anomalies -- AMLs with chromosomal anomalies -- AMLs with gene mutations -- Relationships between genotype and phenotype in cases of AML not recognized as separate entities in WHO 2017 -- AMLs with myelodysplasia-related changes (AML-MRC) -- AMLs not otherwise specified -- AML with minimal differentiation -- AML without maturation -- AML with maturation -- Acute myelomonocytic leukemia (AMMoL) -- Acute monoblastic and monocytic leukemia (AMoL) -- Pure erythroid leukemia (PEL) -- Acute megakaryoblastic leukemia (AMKL) -- Acute basophilic leukemia (ABL) -- Myeloid proliferations associated with Down syndrome -- Transient abnormal myelopoiesis (TAM) -- AMLs in patients with Down syndrome -- Blastic plasmacytoid dendritic cell neoplasm (BPDCN/PDCL) -- Acute leukemias with ambiguous lineage attribution (ALAL) -- Acute undifferentiated leukemias (AUL) -- Mixed phenotype acute leukemias (MPAL) -- Neoplastic diseases of B and T lymphatic precursors -- B lymphoblastic leukemia/lymphoma, not otherwise specified (B-ALL/LBLnos) -- B lymphoblastic leukemia/lymphoma with recurrent genetic anomalies -- Relationships between genotype and phenotype in cases of B-ALL not recognized as separate entities in WHO 2017 -- T lymphoblastic leukemia/lymphoma (T-ALL/LBL) -- Early T-cell precursor lymphoblastic leukemia (ETP-ALL).
NK lymphoblastic leukemia/lymphoma (NK-ALL/LBL) -- Neoplastic diseases of mature B cells -- Chronic lymphocytic leukemia/small lymphocytic lymphoma (B-CLL/SLL) -- Familial B-CLL -- Richter syndrome -- Monoclonal B-cell lymphocytosis (MBL) -- CLL-like monoclonal B lymphocytosis -- Non-CLL-like monoclonal B lymphocytosis -- B-cell prolymphocytic leukemia (B-PLL) -- Lymphoplasmacytic lymphoma (LPL) -- Heavy chain disease (HCD) -- μ heavy chain disease -- α heavy chain disease -- Hairy cell leukemia (HCL) -- Hairy cell leukemia, variant (HCL-v) -- Hairy cell leukemia, Japanese variant (HCL-J) -- Splenic diffuse red pulp lymphoma (SDRPL) -- Marginal zone lymphomas (MZL) -- Nodal marginal zone lymphoma (NMZL) -- Splenic marginal zone lymphoma (SMZL) -- Extranodal marginal zone lymphoma (EMZL/MALToma) -- Clonal B-cell lymphocytosis with MZL-like phenotype (CBL-MZ) -- Follicular lymphoma (FCL) -- Testicular follicular lymphoma -- Duodenal type follicular lymphoma -- Pediatric type follicular lymphoma -- Primitive cutaneous follicular lymphoma (PCFL) -- Large B-cell lymphoma with IRF4 rearrangement -- Mantle-cell lymphoma (MCL) -- Blastic mantle-cell lymphoma (BMCL) -- Leukemic non nodal mantle-cell lymphoma -- DLBCL not otherwise specified (DLBCLnos) -- CD5(+) diffuse large cell lymphoma (CD5(+) DLBCL) -- T-cell/histiocyte-rich B-cell lymphoma (THRLBCL) -- Primary DLBCL of the CNS (PCNSL) -- Primary cutaneous DLBCL, "leg type -- EBV(+) DLBCLnos -- DLBCL associated with chronic inflammation (PAL) -- Fibrin associated DLBCL -- Lymphomatoid granulomatosis (LYG) -- Primary mediastinal B-cell lymphoma (PMBCL) -- Intravascular large B-cell lymphoma (IVBCL) -- ALK-positive large cell lymphoma (ALK(+) LBCL) -- Plasmablastic lymphoma (PBL) -- Primary effusion lymphoma (PEL) -- HHV8-associated lymphoproliferative disorders -- HHV8-positive DLBCL.
HHV8-positive germinotropic lymphoproliferative disorder -- Burkitt lymphoma (BL) -- Burkitt leukemia with immature phenotype -- Burkitt-like lymphoma with 11q aberrations -- High-grade B-cell lymphoma (HGBL) -- Plasma cell neoplasms -- Monoclonal gammopathies of undetermined significance (MGUS) -- Multiple myeloma (MM) -- Plasma cell leukemia (PCL) -- Neoplastic diseases of mature T and NK cells -- T-cell prolymphocytic leukemia (T-PLL) -- T-cell large granular lymphocytic leukemia (T-LGL) -- Chronic lymphoproliferative disorders of NK cells (CLPD-NK/CNKL) -- Aggressive NK-cell leukemia (ANKL) -- Adult T-cell leukemia/lymphoma (ATLL) -- Extranodal NK/T-cell lymphoma, "nasal type" (ENKTL) -- Intestinal T-cell lymphomas (ITCL) -- Enteropathy-associated T-cell lymphoma (EATCL) -- Monomorphic epitheliotropic intestinal T-cell lymphoma (MEITL) -- Indolent gastro-intestinal T lymphoproliferative disorder (indolent GI T-LPD) -- Hepatosplenic T-cell lymphoma (HSTCL) -- Subcutaneous panniculitis-like T-cell lymphoma (SPTCL) -- Mycosis fungoides (MF) -- Sezary syndrome (SS) -- Primary cutaneous CD30(+) lymphoproliferative disorders -- Lymphomatoid papulosis (LyP) -- Primary cutaneous anaplastic T-cell lymphoma (pcALCL) -- Primary cutaneous peripheral T-cell lymphoma (PTCL) -- Primary cutaneous TCRƒÁƒÂ(+) T-cell lymphoma (PCGD-TCL) -- Primary cutaneous CD8(+) aggressive epidermotropic cytotoxic T-cell lymphoma (PCAETL) -- Primary cutaneous acral CD8(+) T-cell lymphoma (PCATCL) -- Primary cutaneous lymphoma of the medium/small CD4(+) T cells (PCSM-TCL) -- Peripheral T-cell lymphoma, not otherwise specified (PTCLnos) -- Nodal lymphomas of follicular T-helper derivation -- Angioimmunoblastic T-cell lymphoma (AITL) -- Follicular T-cell lymphoma (FTCL) -- Nodal PTCL with follicular T-helper phenotype -- Anaplastic large cell lymphoma ALK(+) (ALCL ALK(+)).
Anaplastic large cell lymphoma ALK(-) (ALCL ALK(-)) -- Breast implant.associated anaplastic large cell lymphoma (biaALCL) -- Hodgkin lymphomas -- Classic Hodgkin lymphoma (CHL) -- Nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) -- Neoplastic diseases of histiocytic and dendritic cells -- Histiocytic sarcoma (HS) -- Langerhans cell histiocytosis (LCH) -- Indeterminate dendritic cell tumor (IDCT) -- Interdigitating dendritic cell sarcoma (IDCS) -- Follicular dendritic cell sarcoma (FDCS) -- Erdheim.Chester disease (EDC) -- Chapter 3 Appendix -- Acute leukemias not recognized by the 2017 WHO classification -- Acute leukemia of myeloid/NK precursors (M/NK-AL) -- Acute leukemia of myeloid dendritic cells (MDCL) -- Acute leukemia of Langerhans cells -- Composite lymphomas -- Hypereosinophilic syndrome (HES), lymphocyte variant -- Indolent T lymphoblastic proliferations (iT]LBP) -- Polyclonal lymphocytoses of B lymphocytes -- Persistent polyclonal B]cell lymphocytosis (PPBL) -- Persistent polyclonal CD5(+) B]cell lymphocytosis -- Persistent polyclonal B]cell lymphocytosis, Japanese (hairy) variant -- Polyclonal plasmacytoses -- Small round (blue) cell tumors (SR(B)CT) -- References -- Index -- EULA.
Record Nr. UNINA-9910829962903321
Ortolani C (Claudio)  
Hoboken, New Jersey ; ; Chichester, West Sussex, England : , : Wiley Blackwell, , [2021]
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Flow cytometry today : everything you need to know about flow cytometry / / Claudio Ortolani
Flow cytometry today : everything you need to know about flow cytometry / / Claudio Ortolani
Autore Ortolani C (Claudio)
Pubbl/distr/stampa Cham, Switzerland : , : Springer, , [2023]
Descrizione fisica 1 online resource (556 pages)
Disciplina 910.5
Soggetto topico Flow cytometry
ISBN 9783031108365
9783031108358
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Intro -- Foreword -- Preface -- Contents -- Abbreviations -- Chapter 1: General Principles -- 1.1 Flow Cytometers General Layout -- 1.2 Flow Cytometers Features -- 1.3 Parameters and Signals -- 1.4 Time -- References -- Chapter 2: Signals: Scattering -- 2.1 Forward Scatter (FSC) -- 2.2 Side Scatter (SSC) -- 2.3 Raman Scattering -- 2.4 Depolarized Scatter -- References -- Chapter 3: Signals: Fluorescence, Phosphorescence, Impedance, Extinction -- 3.1 Fluorescence -- 3.1.1 Depolarized Fluorescence -- 3.1.2 Autofluorescence -- 3.2 Phosphorescence -- 3.3 Impedance -- 3.4 Axial Extinction -- References -- Chapter 4: Fluidics -- 4.1 Overview on Fluids -- 4.1.1 Laminar Flow and Turbulent Flow -- 4.1.2 Hydrodynamic Focusing -- 4.2 Cytometer Fluidics -- 4.2.1 Sheath and Core -- 4.2.2 Flow System Components -- 4.2.3 Flow Rate Control -- 4.2.4 Sample Injection -- 4.2.4.1 Sample Differential Control -- 4.2.4.2 Absolute Counts -- 4.2.5 Event-Light Interaction: The Interrogation Point -- 4.2.5.1 In-Cuvette Interaction -- 4.2.5.2 Stream-in-Air Interaction -- 4.2.5.3 Interrogation on an Open Surface -- 4.2.5.4 Systems Based on Acoustic Focusing -- 4.2.5.5 Sheathless Systems -- References -- Chapter 5: Light Sources -- 5.1 Arc Lamps -- 5.2 Lasers -- 5.2.1 Gas Lasers -- 5.2.1.1 Argon Ion Lasers -- 5.2.1.2 Krypton Ion Lasers -- 5.2.1.3 Mixed-Gas Ion Lasers (Argon/Krypton) -- 5.2.1.4 Helium-Neon Atom Lasers -- 5.2.1.5 Helium-Cadmium Ion Lasers -- 5.2.1.6 Helium-Silver and Neon-Copper Metal Vapor Lasers -- 5.2.2 Solid-State Lasers (SSLs) -- 5.2.2.1 Ultraviolet (UV) Emitting SSLs -- Deep UV -- 320 nm -- 355 nm -- 5.2.2.2 Near-Ultraviolet Emitting SSLs -- 5.2.2.3 Violet Emitting SSLs -- 5.2.2.4 Blue Emitting SSLs -- Deep Blue -- Blue-Green -- 5.2.2.5 Green and Yellow-Green Emitting SSLs -- 532 nm -- 552 nm -- 561 nm -- 5.2.2.6 Orange Emitting SSLs.
5.2.2.7 Red Emitting SSLs -- Short Red -- Full Red -- 5.2.2.8 Infrared (IR) Emitting SSLs -- 5.2.2.9 Supercontinuum White Light Emitting SSLs -- 5.2.3 Liquid State Lasers (Dye Lasers) -- 5.3 Light Emitting Diodes (LEDs) -- References -- Chapter 6: Optical Benches -- 6.1 From the Light Source(s) to the Interrogation Point(s) -- 6.2 From the Interrogation Point(s) to the Detector(s) -- 6.3 Optical Bench Components -- 6.3.1 Absorption Filters -- 6.3.2 Interference Filters -- 6.3.3 Neutral Density Filters -- 6.3.4 Polarizing Filters -- 6.3.5 Beam Splitters -- 6.3.6 Wavelength Division Multiplexing (WDM) -- 6.3.7 Prisms, Gratings, Coarse WDM (CWDM) -- 6.4 Optical Bench Layouts -- 6.4.1 Transmission Benches -- 6.4.2 Reflection Benches -- 6.4.3 Multilaser Benches -- 6.4.3.1 Spaced Lasers and Separate Pathways/Detectors -- 6.4.3.2 Spaced Lasers and Shared Pathways/Detectors -- 6.4.3.3 Time Delay -- 6.4.3.4 Continuous Collinear Lasers -- 6.4.3.5 Pulsed Collinear Lasers -- 6.4.4 Special Solutions -- 6.4.4.1 Pie-Shaped Design -- 6.4.4.2 Benches for (De)Polarized Signals -- 6.4.4.3 Spectrally Enhanced Optical Benches -- References -- Chapter 7: Detectors and Electronics -- 7.1 Photodetectors -- 7.1.1 Photodiodes (PDs) -- 7.1.2 Avalanche Photodiodes (APDs) -- 7.1.3 Photomultipliers (PMTs) -- 7.1.4 Multi-anode Photomultipliers -- 7.1.5 Silicon Photomultipliers (SiPMs) -- 7.1.6 Charged-Coupled Devices (CCDs) -- 7.1.7 Trans-impedance Amplifiers (TIAs) -- 7.2 Circuitry -- 7.2.1 Analog Model -- 7.2.1.1 Baseline Restorers -- 7.2.1.2 Comparators and Threshold -- 7.2.1.3 Accessory Circuits -- 7.2.1.4 Amplifiers -- Linear Amplifiers -- Logarithmic Amplifiers -- 7.2.1.5 DC Restorers -- 7.2.1.6 Peak Detectors and Integrators -- Measurement of H (Peak Detectors) -- Measurement of A (Integrators) -- Measurement of W -- Pulse Processing Synchronization.
7.2.1.7 Analog-to-Digital Converters (ADCs) -- Bit Number -- Clock Number -- 7.2.2 Digital Model -- 7.2.2.1 Data Acquisition Boards (DAQs) -- Analog-to-Digital Converters (ADCs) -- Field-Programmable Gate Arrays (FPGAs) -- Digital Signal Processors (DSPs) -- 7.2.2.2 General Considerations on the Digital Model -- 7.2.3 Hybrid Model -- References -- Chapter 8: Signal Analysis -- 8.1 The Background -- 8.1.1 Instrumental Background (BCAL) -- 8.1.2 Experimental Background (Bsos) -- 8.2 The Pulse -- 8.2.1 Pulse Analysis in Analog Systems -- 8.2.2 Pulse Analysis in Digital Systems -- 8.2.2.1 Window´s Gate and Window´s Extension -- 8.2.2.2 Area Scaling -- 8.2.3 Practical Applications of Pulse Analysis -- 8.2.3.1 FSC-A vs. FSC-H vs. FSC-W -- 8.2.3.2 FL-A vs. FL-W -- 8.2.3.3 FL-H vs. FL-W -- 8.3 Dynamic Range of the Signal -- 8.3.1 Effective Resolution -- 8.3.2 Picket Fence Phenomenon -- References -- Chapter 9: The Cytometric File -- 9.1 FCS Format -- 9.2 Segments -- 9.2.1 Header Segment -- 9.2.2 Text Segment -- 9.2.3 Data Segment -- 9.2.4 Analysis Segment -- 9.2.5 Optional Segments -- 9.3 Keywords -- 9.3.1 Standard Keywords -- 9.3.1.1 Required Keywords -- 9.3.1.2 Optional Keywords -- 9.3.2 Non-standard Keywords -- 9.3.3 Relationships Between Keywords and Compensation Procedures -- References -- Chapter 10: Data Transformation -- 10.1 Logarithmic Transformation -- 10.2 Log-Like Transformations -- 10.3 Polynomial Transformation -- References -- Chapter 11: Data Representation -- 11.1 Histogram -- 11.1.1 Histograms of Lin Amplified Data -- 11.1.2 Histograms of Log Amplified/Transformed Data -- 11.1.3 Histograms of Log-Like Transformed Data -- 11.2 Cytograms -- 11.2.1 Representation by Dots (Dot Plot) -- 11.2.2 Representation by Contours (Contour Plot) -- 11.2.2.1 Logarithmic Density -- 11.2.2.2 Probability -- 11.2.3 Representation by False Colors or Gray Tones.
11.2.4 Pseudo-Three-Dimensional Representation -- 11.2.5 Three-Dimensional Representation -- References -- Chapter 12: Data Analysis -- 12.1 Immunofluorescence Measurements -- 12.1.1 The Vexed Question of the Negative Control -- 12.1.1.1 Isotype Control -- 12.1.1.2 Isoclonic Control -- 12.1.1.3 Fluorescence-Minus-One (FMO) Control -- 12.1.1.4 Unstained Control -- 12.1.2 Histograms -- 12.1.2.1 MFI (Mean Fluorescence Intensity) -- 12.1.2.2 RFI (Relative Fluorescence Intensity) -- 12.1.3 Cytograms -- 12.1.4 Weak Positivity in Immunofluorescence -- 12.1.4.1 Weak Positivity with a Negative Component -- 12.1.4.2 Weak Positivity Without an Apparent Negative Component -- 12.2 DNA Content Measurements -- 12.2.1 DNA Content versus BrdU Incorporation -- 12.3 Concept of Gate and Concept of Region -- 12.3.1 Combined (Boolean) Use of Regions and Gates -- 12.3.1.1 In the Determination of Hematopoietic Stem Cells (HSCs) -- ISHAGE Protocol -- 12.3.1.2 In the Determination of the Minimal Residual Disease (MRD) -- 12.3.1.3 In the Augmentation of the Dimensionality in Cell Subset Analysis -- 12.4 Advanced Tools and Future Perspectives -- 12.4.1 Pre-processing Programs -- 12.4.1.1 Quality Assessment -- 12.4.1.2 Data Normalization -- 12.4.1.3 Data Compensation -- 12.4.1.4 Data Transformation -- 12.4.2 Data Processing Programs -- 12.4.2.1 Dimensionality Reduction Based Programs -- 12.4.2.2 Clustering-Based Programs -- References -- Chapter 13: Standards, Setup, Calibration, and Control Techniques -- 13.1 Standards in Flow Cytometry -- 13.1.1 Natural Standards -- 13.1.2 Artificial Standards -- 13.1.2.1 Type 0 Artificial Standards -- 13.1.2.2 Type I Artificial Standards -- 13.1.2.3 Type II Artificial Standards -- Type IIa -- Type IIb -- Type IIc -- 13.1.2.4 Type III Artificial Standards -- Type IIIa -- Type IIIb -- Type IIIc Standards in Daily Practice.
13.1.2.5 Primary Performance Parameters (PPP) -- 13.1.3 Standard Use in Quality Procedures -- 13.1.3.1 Internal Quality Controls (IQCs) -- 13.1.3.2 External Quality Assessments (EQAs) -- 13.2 Optical Bench Setup -- 13.3 Photodetectors´ Setup -- 13.3.1 SDen (Electronic Noise Standard Deviation) -- 13.3.2 PMT Setup -- 13.3.3 APD Setup -- 13.4 Calibration -- 13.4.1 Calibration in ERF -- 13.4.2 Calibration in MESF -- 13.4.3 Calibration in ABC -- 13.4.3.1 With Conjugated Antibodies -- 13.4.3.2 With Unconjugated Antibodies -- 13.4.4 Calibration in FLU -- 13.4.5 Calibration in Nanometers -- 13.5 Instrument Performance and Its Control -- 13.5.1 Linearity -- 13.5.2 Accuracy -- 13.5.2.1 Carry-over -- 13.5.2.2 Count Inaccuracy at High Speed -- 13.5.3 Resolution -- 13.5.4 Sensitivity -- 13.5.4.1 Q, Qr, Stain Index, and Other Indexes -- 13.5.4.2 Antibody Titration -- 13.5.5 Limits of Blank (LOB), Detection (LOD), and Quantification (LOQ) -- 13.5.5.1 LOB, LOD, and LOQ in the Detection of Weak Signals -- LOB (Limit of Blank) -- LOD (Limit of Detection) -- LOQ (Limit of Quantification) -- 13.5.5.2 LOB, LOD, and LOQ in Rare Event Analysis -- LOB (Limit of Blank) -- LOD (Limit of Detection) -- LOQ (Limit of Quantification) -- LOD and LOQ and Minimal Residual Disease (MRD) -- 13.5.6 Precision -- 13.5.7 Specificity -- References -- Chapter 14: Fluorochromes: Overview -- 14.1 Spectral Behavior of Fluorescent Molecules -- 14.2 Relationships with the Environment -- 14.2.1 Spectral Effects -- 14.2.1.1 Bathochromic Effect -- 14.2.1.2 Hypsochromic Effect -- 14.2.1.3 Hyperchromic Effect -- 14.2.1.4 Hypochromic Effect -- 14.2.1.5 Solvatochromic Effect -- 14.2.2 Other Effects -- 14.2.2.1 Extinction or Quenching -- 14.2.2.2 Photodestruction or Photobleaching -- 14.2.2.3 Non-radiative Transfer of Energy (FRET) -- 14.3 Accessory Groups -- References.
Chapter 15: Fluorochromes Suitable for Antibody Conjugation.
Record Nr. UNINA-9910631093503321
Ortolani C (Claudio)  
Cham, Switzerland : , : Springer, , [2023]
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