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Molecular biology in cellular pathology [[electronic resource] /] / edited by John Crocker, Paul G. Murray
Molecular biology in cellular pathology [[electronic resource] /] / edited by John Crocker, Paul G. Murray
Pubbl/distr/stampa Chichester, West Sussex, England ; ; Hoboken, NJ, : John Wiley & Sons, c2003
Descrizione fisica 1 online resource (403 p.)
Disciplina 611.018
611.0181
Altri autori (Persone) CrockerJ
MurrayPaul, Ph. D.
Soggetto topico Pathology, Molecular
Pathology, Cellular
ISBN 1-280-27200-7
9786610272006
0-470-29924-X
0-470-86498-2
0-470-86794-9
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Molecular Biology in Cellular Pathology; Contents; Preface; Preface to Molecular Biology in Histopathology; List of Contributors; 1 Blotting Techniques: Methodology and Applications; 1.1 Introduction; 1.2 Blotting techniques; 1.3 References; 2 In-situ Hybridisation in Histopathology; 2.1 Introduction; 2.2 Experimental conditions; 2.3 Probes and labels; 2.4 Controls and pitfalls; 2.5 Double-labelling; 2.6 Increasing the sensitivity of ISH; 2.7 What we do in our laboratories; 2.8 Applications of ISH: examples; 2.9 Perspective; 2.10 References; 3 DNA Flow Cytometry; 3.1 Introduction
3.2 Definitions and terms3.3 Dye used for DNA analysis; 3.4 Sample preparation for DNA analysis; 3.5 Analysis of the DNA histogram; 3.6 Quality control; 3.7 Computer analysis of the DNA histogram; 3.8 Multiparametric measurement; 3.9 Acknowledgements; 3.10 References; 4 Interphase Cytogenetics; 4.1 Introduction; 4.2 Interphase cytogenetics; 4.3 Applications; 4.4 Conclusion; 4.5 References; 5 Oncogenes; 5.1 Introduction; 5.2 Identification of the oncogenes; 5.3 Functions of the proto-oncogenes; 5.4 Mechanism of oncogene activation; 5.5 Oncogenes in colorectal cancer
5.6 Oncogenes in breast cancer5.7 Oncogenes in lung cancer; 5.8 Oncogenes in haematological malignancies; 5.9 Other cancers; 5.10 Conclusion; 5.11 References; 6 Molecular and Immunological Aspects of Cell Proliferation; 6.1 The cell cycle and its importance in clinical pathology; 6.2 Molecular control of the cell cycle; 6.3 Cell cycle control; 6.4 The cell cycle and cancer; 6.5 Immunocytochemical markers of proliferating cells; 6.6 References; 6.7 Further Reading; 7 Interphase Nucleolar Organiser Regions in Tumour Pathology; 7.1 Introduction; 7.2 The AgNORs; 7.3 NOR silver-staining
7.4 Quantitative AgNOR analysis7.5 AgNORs as a parameter of the level of cell proliferation; 7.6 Application of the AgNOR technique to tumour pathology; 7.7 What future for AgNORs in tumour pathology?; 7.8 References; 8 Apoptosis and Cell Senescence; 8.1 Introduction; 8.2 Apoptosis; 8.3 Cell senescence; 8.4 Summary; 8.5 References; 9 The Polymerase Chain Reaction; 9.1 Introduction; 9.2 Principles; 9.3 Analysis of products; 9.4 RT-PCR; 9.5 Quantitative PCR; 9.6 DNA and RNA extraction; 9.7 Correlation of the PCR with morphology; 9.8 Problems; 9.9 Applications; 9.10 Diagnostic applications
9.11 Infectious diseases9.12 Identity; 9.13 The future; 9.14 References; 9.15 Online information; 10 Laser Capture Microdissection: Techniques and Applications in the Molecular Analysis of the Cancer Cell; 10.1 Introduction; 10.2 The principle of LCM; 10.3 Technical considerations; 10.4 Advantages and disadvantages of LCM; 10.5 Applications of LCM; 10.6 Future perspectives; 10.7 Acknowledgements; 10.8 References; 11 The In-situ Polymerase Chain Reaction; 11.1 Introduction; 11.2 Overview of the methodology; 11.3 In-cell PCR technologies; 11.4 In-cell amplification of DNA
11.5 Detection of amplicons
Record Nr. UNINA-9910143169003321
Chichester, West Sussex, England ; ; Hoboken, NJ, : John Wiley & Sons, c2003
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Molecular biology in cellular pathology [[electronic resource] /] / edited by John Crocker, Paul G. Murray
Molecular biology in cellular pathology [[electronic resource] /] / edited by John Crocker, Paul G. Murray
Pubbl/distr/stampa Chichester, West Sussex, England ; ; Hoboken, NJ, : John Wiley & Sons, c2003
Descrizione fisica 1 online resource (403 p.)
Disciplina 611.018
611.0181
Altri autori (Persone) CrockerJ
MurrayPaul, Ph. D.
Soggetto topico Pathology, Molecular
Pathology, Cellular
ISBN 1-280-27200-7
9786610272006
0-470-29924-X
0-470-86498-2
0-470-86794-9
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Molecular Biology in Cellular Pathology; Contents; Preface; Preface to Molecular Biology in Histopathology; List of Contributors; 1 Blotting Techniques: Methodology and Applications; 1.1 Introduction; 1.2 Blotting techniques; 1.3 References; 2 In-situ Hybridisation in Histopathology; 2.1 Introduction; 2.2 Experimental conditions; 2.3 Probes and labels; 2.4 Controls and pitfalls; 2.5 Double-labelling; 2.6 Increasing the sensitivity of ISH; 2.7 What we do in our laboratories; 2.8 Applications of ISH: examples; 2.9 Perspective; 2.10 References; 3 DNA Flow Cytometry; 3.1 Introduction
3.2 Definitions and terms3.3 Dye used for DNA analysis; 3.4 Sample preparation for DNA analysis; 3.5 Analysis of the DNA histogram; 3.6 Quality control; 3.7 Computer analysis of the DNA histogram; 3.8 Multiparametric measurement; 3.9 Acknowledgements; 3.10 References; 4 Interphase Cytogenetics; 4.1 Introduction; 4.2 Interphase cytogenetics; 4.3 Applications; 4.4 Conclusion; 4.5 References; 5 Oncogenes; 5.1 Introduction; 5.2 Identification of the oncogenes; 5.3 Functions of the proto-oncogenes; 5.4 Mechanism of oncogene activation; 5.5 Oncogenes in colorectal cancer
5.6 Oncogenes in breast cancer5.7 Oncogenes in lung cancer; 5.8 Oncogenes in haematological malignancies; 5.9 Other cancers; 5.10 Conclusion; 5.11 References; 6 Molecular and Immunological Aspects of Cell Proliferation; 6.1 The cell cycle and its importance in clinical pathology; 6.2 Molecular control of the cell cycle; 6.3 Cell cycle control; 6.4 The cell cycle and cancer; 6.5 Immunocytochemical markers of proliferating cells; 6.6 References; 6.7 Further Reading; 7 Interphase Nucleolar Organiser Regions in Tumour Pathology; 7.1 Introduction; 7.2 The AgNORs; 7.3 NOR silver-staining
7.4 Quantitative AgNOR analysis7.5 AgNORs as a parameter of the level of cell proliferation; 7.6 Application of the AgNOR technique to tumour pathology; 7.7 What future for AgNORs in tumour pathology?; 7.8 References; 8 Apoptosis and Cell Senescence; 8.1 Introduction; 8.2 Apoptosis; 8.3 Cell senescence; 8.4 Summary; 8.5 References; 9 The Polymerase Chain Reaction; 9.1 Introduction; 9.2 Principles; 9.3 Analysis of products; 9.4 RT-PCR; 9.5 Quantitative PCR; 9.6 DNA and RNA extraction; 9.7 Correlation of the PCR with morphology; 9.8 Problems; 9.9 Applications; 9.10 Diagnostic applications
9.11 Infectious diseases9.12 Identity; 9.13 The future; 9.14 References; 9.15 Online information; 10 Laser Capture Microdissection: Techniques and Applications in the Molecular Analysis of the Cancer Cell; 10.1 Introduction; 10.2 The principle of LCM; 10.3 Technical considerations; 10.4 Advantages and disadvantages of LCM; 10.5 Applications of LCM; 10.6 Future perspectives; 10.7 Acknowledgements; 10.8 References; 11 The In-situ Polymerase Chain Reaction; 11.1 Introduction; 11.2 Overview of the methodology; 11.3 In-cell PCR technologies; 11.4 In-cell amplification of DNA
11.5 Detection of amplicons
Record Nr. UNINA-9910830360303321
Chichester, West Sussex, England ; ; Hoboken, NJ, : John Wiley & Sons, c2003
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Molecular biology in cellular pathology / / edited by John Crocker, Paul G. Murray
Molecular biology in cellular pathology / / edited by John Crocker, Paul G. Murray
Pubbl/distr/stampa Chichester, West Sussex, England ; ; Hoboken, NJ, : John Wiley & Sons, c2003
Descrizione fisica 1 online resource (403 p.)
Disciplina 611/.018
Altri autori (Persone) CrockerJ
MurrayPaul, Ph. D.
Soggetto topico Pathology, Molecular
Pathology, Cellular
ISBN 9786610272006
9781280272004
1280272007
9780470299241
047029924X
9780470864982
0470864982
9780470867945
0470867949
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Molecular Biology in Cellular Pathology; Contents; Preface; Preface to Molecular Biology in Histopathology; List of Contributors; 1 Blotting Techniques: Methodology and Applications; 1.1 Introduction; 1.2 Blotting techniques; 1.3 References; 2 In-situ Hybridisation in Histopathology; 2.1 Introduction; 2.2 Experimental conditions; 2.3 Probes and labels; 2.4 Controls and pitfalls; 2.5 Double-labelling; 2.6 Increasing the sensitivity of ISH; 2.7 What we do in our laboratories; 2.8 Applications of ISH: examples; 2.9 Perspective; 2.10 References; 3 DNA Flow Cytometry; 3.1 Introduction
3.2 Definitions and terms3.3 Dye used for DNA analysis; 3.4 Sample preparation for DNA analysis; 3.5 Analysis of the DNA histogram; 3.6 Quality control; 3.7 Computer analysis of the DNA histogram; 3.8 Multiparametric measurement; 3.9 Acknowledgements; 3.10 References; 4 Interphase Cytogenetics; 4.1 Introduction; 4.2 Interphase cytogenetics; 4.3 Applications; 4.4 Conclusion; 4.5 References; 5 Oncogenes; 5.1 Introduction; 5.2 Identification of the oncogenes; 5.3 Functions of the proto-oncogenes; 5.4 Mechanism of oncogene activation; 5.5 Oncogenes in colorectal cancer
5.6 Oncogenes in breast cancer5.7 Oncogenes in lung cancer; 5.8 Oncogenes in haematological malignancies; 5.9 Other cancers; 5.10 Conclusion; 5.11 References; 6 Molecular and Immunological Aspects of Cell Proliferation; 6.1 The cell cycle and its importance in clinical pathology; 6.2 Molecular control of the cell cycle; 6.3 Cell cycle control; 6.4 The cell cycle and cancer; 6.5 Immunocytochemical markers of proliferating cells; 6.6 References; 6.7 Further Reading; 7 Interphase Nucleolar Organiser Regions in Tumour Pathology; 7.1 Introduction; 7.2 The AgNORs; 7.3 NOR silver-staining
7.4 Quantitative AgNOR analysis7.5 AgNORs as a parameter of the level of cell proliferation; 7.6 Application of the AgNOR technique to tumour pathology; 7.7 What future for AgNORs in tumour pathology?; 7.8 References; 8 Apoptosis and Cell Senescence; 8.1 Introduction; 8.2 Apoptosis; 8.3 Cell senescence; 8.4 Summary; 8.5 References; 9 The Polymerase Chain Reaction; 9.1 Introduction; 9.2 Principles; 9.3 Analysis of products; 9.4 RT-PCR; 9.5 Quantitative PCR; 9.6 DNA and RNA extraction; 9.7 Correlation of the PCR with morphology; 9.8 Problems; 9.9 Applications; 9.10 Diagnostic applications
9.11 Infectious diseases9.12 Identity; 9.13 The future; 9.14 References; 9.15 Online information; 10 Laser Capture Microdissection: Techniques and Applications in the Molecular Analysis of the Cancer Cell; 10.1 Introduction; 10.2 The principle of LCM; 10.3 Technical considerations; 10.4 Advantages and disadvantages of LCM; 10.5 Applications of LCM; 10.6 Future perspectives; 10.7 Acknowledgements; 10.8 References; 11 The In-situ Polymerase Chain Reaction; 11.1 Introduction; 11.2 Overview of the methodology; 11.3 In-cell PCR technologies; 11.4 In-cell amplification of DNA
11.5 Detection of amplicons
Record Nr. UNINA-9911019655603321
Chichester, West Sussex, England ; ; Hoboken, NJ, : John Wiley & Sons, c2003
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui