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Bioanalytical chemistry / / Susan R. Mikkelsen, Eduardo Cortón
| Bioanalytical chemistry / / Susan R. Mikkelsen, Eduardo Cortón |
| Autore | Mikkelsen Susan R. <1960-> |
| Edizione | [Second edition.] |
| Pubbl/distr/stampa | Hoboken, New Jersey : , : John Wiley & Sons, Incorporated, , [2016] |
| Descrizione fisica | 1 online resource (641 p.) |
| Disciplina | 612/.01585 |
| Soggetto topico |
Analytical biochemistry
Chemistry, Analytic |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-119-05770-1
1-119-05763-9 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Title Page; Copyright; Preface to Second Edition; Preface to First Edition; Acknowledgments; 1: Quantitative Instrumental Measurements; 1.1 Introduction; 1.2 Optical Measurements; 1.3 Electrochemical Measurements; 1.4 Radiochemical Measurements; 1.5 Surface Plasmon Resonance; 1.6 Calorimetry; 1.7 Automation: Microplates, Multiwell Liquid Dispensers and Microplate Readers; 1.8 Calibration of Instrumental Measurements; 1.9 Quantitative and Semi-Quantitative Measurements; Suggested Reading; Problems; 2: Spectroscopic Methods for the Quantitation of Classes of Biomolecules; 2.1 Introduction
2.2 Total Protein2.3 Total DNA; 2.4 Total RNA; 2.5 Total Carbohydrate; 2.6 Free Fatty Acids; References; Problems; 3: Enzymes; 3.1 Introduction; 3.2 Enzyme Nomenclature; 3.3 Enzyme Commission Numbers; 3.4 Enzymes in Bioanalytical Chemistry; 3.5 Enzyme Kinetics; 3.6 Enzyme Activators; 3.7 Enzyme Inhibitors; 3.8 Enzyme Units and Concentrations; Suggested Reading; References; Problems; 4: Quantitation of Enzymes and Their Substrates; 4.1 Introduction; 4.2 Substrate Depletion or Product Accumulation; 4.3 Direct and Coupled Measurements; 4.4 Classification of Methods; 4.5 Instrumental Methods 4.6 High-Throughput Assays for Enzymes and Inhibitors4.7. Assays for Enzymatic Reporter Gene Products27-30; 4.8 Practical Considerations for Enzymatic Assays; Suggested Reading; References; Problems; 5: Immobilized Enzymes; 5.1 Introduction; 5.2 Immobilization Methods; 5.3 Properties of Immobilized Enzymes; 5.4 Immobilized Enzyme Reactors; 5.5 Theoretical Treatment of Packed-Bed Enzyme Reactors34-37; Suggested Reading; References; Problems; 6: Antibodies; 6.1 Introduction; 6.2 Structural and Functional Properties of Antibodies; 6.3 Polyclonal and Monoclonal Antibodies 6.4 Antibody-Antigen Interactions6.5 Analytical Applications of Secondary Antibody-Antigen Interactions; Suggested Reading; References; Problems; 7: Quantitative Immunoassays with Labels; 7.1 Introduction; 7.2 Labeling Reactions; 7.3 Heterogeneous Immunoassays; 7.4 Homogeneous Immunoassays; 7.5 Evaluation of New Immunoassay Methods33,34; Suggested Reading; References; Problems; 8: Biosensors; 8.1 Introduction; 8.2 Biosensor Diversity and Classification; 8.3 Recognition Agents; 8.4 Response of Enzyme-Based Biosensors5; 8.5 Examples of Biosensor Configurations 8.6 Evaluation of Biosensor Perfomance8.7 In VIVO Applications of Biosensors; Suggested Reading; References; Problems; 9: Directed Evolution for the Design of Macromolecular Reagents; 9.1 Introduction; 9.2 Rational Design and Directed Evolution; 9.3 Generation of Genetic Diversity; 9.4 Linking Genotype and Phenotype; 9.5 Identification and Selection of Successful Variants; 9.6 Examples of Directed Evolution Experiments; Suggested Reading; References; Problems; 10: Image-Based Bioanalysis; 10.1 Introduction; 10.2 Magnification and Resolution; 10.3 Optical Microscopy; 10.4 Electron Microscopy 10.5 Scanning Tunneling Microscopy |
| Record Nr. | UNINA-9910465872703321 |
Mikkelsen Susan R. <1960->
|
||
| Hoboken, New Jersey : , : John Wiley & Sons, Incorporated, , [2016] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Bioanalytical chemistry / / Susan R. Mikkelsen, Eduardo Corton
| Bioanalytical chemistry / / Susan R. Mikkelsen, Eduardo Corton |
| Autore | Mikkelsen Susan R. <1960-> |
| Edizione | [Second edition.] |
| Pubbl/distr/stampa | Hoboken, New Jersey : , : John Wiley & Sons, Incorporated, , [2016] |
| Descrizione fisica | 1 online resource (641 pages) : illustrations |
| Disciplina | 612/.01585 |
| Soggetto topico |
Analytical biochemistry
Chemistry, Analytic |
| ISBN |
1-119-05763-9
1-119-05770-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910795945303321 |
|
Mikkelsen Susan R. <1960->
|
||
| Hoboken, New Jersey : , : John Wiley & Sons, Incorporated, , [2016] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Bioanalytical chemistry / / Susan R. Mikkelsen, Eduardo Corton
| Bioanalytical chemistry / / Susan R. Mikkelsen, Eduardo Corton |
| Autore | Mikkelsen Susan R. <1960-> |
| Edizione | [Second edition.] |
| Pubbl/distr/stampa | Hoboken, New Jersey : , : John Wiley & Sons, Incorporated, , [2016] |
| Descrizione fisica | 1 online resource (641 pages) : illustrations |
| Disciplina | 612/.01585 |
| Soggetto topico |
Analytical biochemistry
Chemistry, Analytic |
| ISBN |
1-119-05763-9
1-119-05770-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910814575103321 |
|
Mikkelsen Susan R. <1960->
|
||
| Hoboken, New Jersey : , : John Wiley & Sons, Incorporated, , [2016] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Bioanalytical chemistry [[electronic resource] /] / Susan R. Mikkelsen, Eduardo Cortón
| Bioanalytical chemistry [[electronic resource] /] / Susan R. Mikkelsen, Eduardo Cortón |
| Autore | Mikkelsen Susan R. <1960-> |
| Pubbl/distr/stampa | Hoboken, N.J., : John Wiley & Sons, c2004 |
| Descrizione fisica | 1 online resource (381 p.) |
| Disciplina | 572/.36 |
| Altri autori (Persone) | CortónEduardo <1962-> |
| Soggetto topico | Analytical biochemistry |
| ISBN |
1-280-36794-6
9786610367948 0-470-23229-3 0-471-62386-5 0-471-62362-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
BIOANALYTICAL CHEMISTRY; CONTENTS; Preface; Acknowledgments; 1. Spectroscopic Methods for Matrix Characterization; 1.1 Introduction; 1.2 Total Protein; 1.2.1 Lowry Method; 1.2.2 Smith (BCA) Method; 1.2.3 Bradford Method; 1.2.4 Ninhydrin-Based Assay; 1.2.5 Other Protein Quantitation Methods; 1.3 Total DNA; 1.3.1 Diaminobenzoic Acid Method; 1.3.2 Diphenylamine Method; 1.3.3 Other Fluorometric Methods; 1.4 Total RNA; 1.5 Total Carbohydrate; 1.5.1 Ferricyanide Method; 1.5.2 Phenol-Sulfuric Acid Method; 1.5.3 2-Aminothiophenol Method; 1.5.4 Purpald Assay for Bacterial Polysaccharides
1.6 Free Fatty AcidsReferences; Problems; 2. Enzymes; 2.1 Introduction; 2.2 Enzyme Nomenclature; 2.3 Enzyme Commission Numbers; 2.4 Enzymes in Bioanalytical Chemistry; 2.5 Enzyme Kinetics; 2.5.1 Simple One-Substrate Enzyme Kinetics; 2.5.2 Experimental Determination of Michaelis-Menten Parameters; 2.5.2.1 Eadie-Hofstee Method; 2.5.2.2 Hanes Method; 2.5.2.3 Lineweaver-Burk Method; 2.5.2.4 Cornish-Bowden-Eisenthal Method; 2.5.3 Comparison of Methods for the Determination of K(m) Values; 2.5.4 One-Substrate, Two-Product Enzyme Kinetics; 2.5.5 Two-Substrate Enzyme Kinetics 2.5.6 Examples of Enzyme-Catalyzed Reactions and Their Treatment2.6 Enzyme Activators; 2.7 Enzyme Inhibitors; 2.7.1 Competitive Inhibition; 2.7.2 Noncompetitive Inhibition; 2.7.3 Uncompetitive Inhibition; 2.8 Enzyme Units and Concentrations; Suggested References; References; Problems; 3. Quantitation of Enzymes and Their Substrates; 3.1 Introduction; 3.2 Substrate Depletion or Product Accumulation; 3.3 Direct and Coupled Measurements; 3.4 Classification of Methods; 3.5 Instrumental Methods; 3.5.1 Optical Detection; 3.5.1.1 Absorbance; 3.5.1.2 Fluorescence; 3.5.1.3 Luminescence 3.5.1.4 Nephelometry3.5.2 Electrochemical Detection; 3.5.2.1 Amperometry; 3.5.2.2 Potentiometry; 3.5.2.3 Conductimetry; 3.5.3 Other Detection Methods; 3.5.3.1 Radiochemical; 3.5.3.2 Manometry; 3.5.3.3 Calorimetry; 3.6 Ultra-High-Throughput Assays (HTA); 3.7 Practical Considerations for Enzymatic Assays; Suggested References; References; Problems; 4. Immobilized Enzymes; 4.1 Introduction; 4.2 Immobilization Methods; 4.2.1 Nonpolymerizing Covalent Immobilization; 4.2.1.1 Controlled-Pore Glass; 4.2.1.2 Polysaccharides; 4.2.1.3 Polyacrylamide; 4.2.1.4 Acidic Supports; 4.2.1.5 Anhydride Groups 4.2.1.6 Thiol Groups4.2.2 Cross-Linking with Bifunctional Reagents; 4.2.3 Adsorption; 4.2.4 Entrapment; 4.2.5 Microencapsulation; 4.3 Properties of Immobilized Enzymes; 4.4 Immobilized Enzyme Reactors; 4.5 Theoretical Treatment of Packed-Bed Enzyme Reactors; Suggested References; References; Problems; 5. Antibodies; 5.1 Introduction; 5.2 Structural and Functional Properties of Antibodies; 5.3 Polyclonal and Monoclonal Antibodies; 5.4 Antibody-Antigen Interactions; 5.5 Analytical Applications of Secondary Antibody-Antigen Interactions; 5.5.1 Agglutination Reactions 5.5.2 Precipitation Reactions |
| Record Nr. | UNINA-9910780374003321 |
|
Mikkelsen Susan R. <1960->
|
||
| Hoboken, N.J., : John Wiley & Sons, c2004 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Bioanalytical chemistry / / Susan R. Mikkelsen, Eduardo Corton
| Bioanalytical chemistry / / Susan R. Mikkelsen, Eduardo Corton |
| Autore | Mikkelsen Susan R. <1960-> |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : John Wiley & Sons, c2004 |
| Descrizione fisica | 1 online resource (381 p.) |
| Disciplina | 572/.36 |
| Altri autori (Persone) | CortonEduardo <1962-> |
| Soggetto topico | Analytical biochemistry |
| ISBN |
1-280-36794-6
9786610367948 0-470-23229-3 0-471-62386-5 0-471-62362-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
BIOANALYTICAL CHEMISTRY; CONTENTS; Preface; Acknowledgments; 1. Spectroscopic Methods for Matrix Characterization; 1.1 Introduction; 1.2 Total Protein; 1.2.1 Lowry Method; 1.2.2 Smith (BCA) Method; 1.2.3 Bradford Method; 1.2.4 Ninhydrin-Based Assay; 1.2.5 Other Protein Quantitation Methods; 1.3 Total DNA; 1.3.1 Diaminobenzoic Acid Method; 1.3.2 Diphenylamine Method; 1.3.3 Other Fluorometric Methods; 1.4 Total RNA; 1.5 Total Carbohydrate; 1.5.1 Ferricyanide Method; 1.5.2 Phenol-Sulfuric Acid Method; 1.5.3 2-Aminothiophenol Method; 1.5.4 Purpald Assay for Bacterial Polysaccharides
1.6 Free Fatty AcidsReferences; Problems; 2. Enzymes; 2.1 Introduction; 2.2 Enzyme Nomenclature; 2.3 Enzyme Commission Numbers; 2.4 Enzymes in Bioanalytical Chemistry; 2.5 Enzyme Kinetics; 2.5.1 Simple One-Substrate Enzyme Kinetics; 2.5.2 Experimental Determination of Michaelis-Menten Parameters; 2.5.2.1 Eadie-Hofstee Method; 2.5.2.2 Hanes Method; 2.5.2.3 Lineweaver-Burk Method; 2.5.2.4 Cornish-Bowden-Eisenthal Method; 2.5.3 Comparison of Methods for the Determination of K(m) Values; 2.5.4 One-Substrate, Two-Product Enzyme Kinetics; 2.5.5 Two-Substrate Enzyme Kinetics 2.5.6 Examples of Enzyme-Catalyzed Reactions and Their Treatment2.6 Enzyme Activators; 2.7 Enzyme Inhibitors; 2.7.1 Competitive Inhibition; 2.7.2 Noncompetitive Inhibition; 2.7.3 Uncompetitive Inhibition; 2.8 Enzyme Units and Concentrations; Suggested References; References; Problems; 3. Quantitation of Enzymes and Their Substrates; 3.1 Introduction; 3.2 Substrate Depletion or Product Accumulation; 3.3 Direct and Coupled Measurements; 3.4 Classification of Methods; 3.5 Instrumental Methods; 3.5.1 Optical Detection; 3.5.1.1 Absorbance; 3.5.1.2 Fluorescence; 3.5.1.3 Luminescence 3.5.1.4 Nephelometry3.5.2 Electrochemical Detection; 3.5.2.1 Amperometry; 3.5.2.2 Potentiometry; 3.5.2.3 Conductimetry; 3.5.3 Other Detection Methods; 3.5.3.1 Radiochemical; 3.5.3.2 Manometry; 3.5.3.3 Calorimetry; 3.6 Ultra-High-Throughput Assays (HTA); 3.7 Practical Considerations for Enzymatic Assays; Suggested References; References; Problems; 4. Immobilized Enzymes; 4.1 Introduction; 4.2 Immobilization Methods; 4.2.1 Nonpolymerizing Covalent Immobilization; 4.2.1.1 Controlled-Pore Glass; 4.2.1.2 Polysaccharides; 4.2.1.3 Polyacrylamide; 4.2.1.4 Acidic Supports; 4.2.1.5 Anhydride Groups 4.2.1.6 Thiol Groups4.2.2 Cross-Linking with Bifunctional Reagents; 4.2.3 Adsorption; 4.2.4 Entrapment; 4.2.5 Microencapsulation; 4.3 Properties of Immobilized Enzymes; 4.4 Immobilized Enzyme Reactors; 4.5 Theoretical Treatment of Packed-Bed Enzyme Reactors; Suggested References; References; Problems; 5. Antibodies; 5.1 Introduction; 5.2 Structural and Functional Properties of Antibodies; 5.3 Polyclonal and Monoclonal Antibodies; 5.4 Antibody-Antigen Interactions; 5.5 Analytical Applications of Secondary Antibody-Antigen Interactions; 5.5.1 Agglutination Reactions 5.5.2 Precipitation Reactions |
| Record Nr. | UNINA-9910815439603321 |
|
Mikkelsen Susan R. <1960->
|
||
| Hoboken, N.J., : John Wiley & Sons, c2004 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||