Info
- Utilizzare la checkbox di selezione a fianco di ciascun documento per attivare le funzionalità di stampa, invio email, download nei formati disponibili del (i) record.
Buying and selling laboratory instruments [[electronic resource] ] : a practical consulting guide / / Marvin C. McMaster
| Buying and selling laboratory instruments [[electronic resource] ] : a practical consulting guide / / Marvin C. McMaster |
| Autore | McMaster Marvin C |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley, 2010 |
| Descrizione fisica | 1 online resource (xiv, 160 p.) : ill |
| Disciplina | 681.750688 |
| Soggetto topico |
Scientific apparatus and instruments - Marketing
Laboratories - Equipment and supplies - Marketing |
| ISBN |
9780470615935 (e-book)
9780470404010 (hbk.) |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | PART ONE PURCHASING LABORATORY INSTRUMENTS; 1 Selecting Laboratory Instruments; 2 Step-By-Step Purchasing; 3 Analytical Instrument Specifications; 4 Finding The Best Price; 5 Grants and Bidding; 6 Instrument Vendor Support; 7 Laboratory Instrument Service; 8 Recycling The System; PART TWO A GUIDE TO THE SELLING PROCESS; 9 Buying Relationships; 10 Sales Justification; 11 Profiling the Sales Call; 12 Objections in the Sales Process; 13 Step-by-Step Instrument Selling; 14 Closing the Sale; 15 The Laws of Selling; 16 Handling Problems; Appendix A Frequently Asked Questions A.1 Frequently Asked Purchasing Questions; A.2 Frequently Asked Questions About New Instruments; A.3 Frequently Asked Questions about the Selling Process; Appendix B Memory Aids, Figures, and Tables; Appendix C Glossary of Purchasing and Sales Terms; Appendix D Troubleshooting Quick Reference; D.1 Troubleshooting the Purchase; D.2 Troubleshooting the Sale; Appendix E Selected Reading List; INDEX. |
| Record Nr. | UNINA-9910140821903321 |
McMaster Marvin C
|
||
| Hoboken, N.J., : Wiley, 2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Buying and selling laboratory instruments [[electronic resource] ] : a practical consulting guide / / Marvin C. McMaster
| Buying and selling laboratory instruments [[electronic resource] ] : a practical consulting guide / / Marvin C. McMaster |
| Autore | McMaster Marvin C |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley, 2010 |
| Descrizione fisica | 1 online resource (xiv, 160 p.) : ill |
| Disciplina | 681.750688 |
| Soggetto topico |
Scientific apparatus and instruments - Marketing
Laboratories - Equipment and supplies - Marketing |
| ISBN |
9780470615935 (e-book)
9780470404010 (hbk.) |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | PART ONE PURCHASING LABORATORY INSTRUMENTS; 1 Selecting Laboratory Instruments; 2 Step-By-Step Purchasing; 3 Analytical Instrument Specifications; 4 Finding The Best Price; 5 Grants and Bidding; 6 Instrument Vendor Support; 7 Laboratory Instrument Service; 8 Recycling The System; PART TWO A GUIDE TO THE SELLING PROCESS; 9 Buying Relationships; 10 Sales Justification; 11 Profiling the Sales Call; 12 Objections in the Sales Process; 13 Step-by-Step Instrument Selling; 14 Closing the Sale; 15 The Laws of Selling; 16 Handling Problems; Appendix A Frequently Asked Questions A.1 Frequently Asked Purchasing Questions; A.2 Frequently Asked Questions About New Instruments; A.3 Frequently Asked Questions about the Selling Process; Appendix B Memory Aids, Figures, and Tables; Appendix C Glossary of Purchasing and Sales Terms; Appendix D Troubleshooting Quick Reference; D.1 Troubleshooting the Purchase; D.2 Troubleshooting the Sale; Appendix E Selected Reading List; INDEX. |
| Record Nr. | UNINA-9910826619603321 |
|
McMaster Marvin C
|
||
| Hoboken, N.J., : Wiley, 2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
HPLC, a practical user's guide [[electronic resource] /] / Marvin C. McMaster
| HPLC, a practical user's guide [[electronic resource] /] / Marvin C. McMaster |
| Autore | McMaster Marvin C |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Interscience, c2007 |
| Descrizione fisica | 1 online resource (254 p.) |
| Disciplina |
543.84
543/.84 615.1901 |
| Soggetto topico | High performance liquid chromatography |
| ISBN |
1-280-72157-X
9786610721573 0-470-07908-8 0-470-07909-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
HPLC; CONTENTS; PREFACE; I HPLC PRIMER; 1 Advantages and Disadvantages of HPLC; 1.1 How It Works; 1.1.1 A Separation Model of the Column; 1.1.2 Basic Hardware: A Quick, First Look; 1.1.3 Use of Solvent Gradients; 1.1.4 Ranges of Compounds; 1.2 Other Ways to Make My Separation; 1.2.1 FPLC-Fast Protein Liquid Chromatography; 1.2.2 LC-Traditional Liquid Chromatography; 1.2.3 GLC-Gas Liquid Chromatography; 1.2.4 SFC-Supercritical Fluid Chromatography; 1.2.5 TLC-Thin Layer Chromatography; 1.2.6 EP-Electrophoresis; 1.2.7 CZE-Capillary Zone Electrophoresis; 2 Selecting an HPLC System
2.1 Characteristic Systems2.1.1 Finding a Fit: Detectors and Data Processing; 2.1.2 System Models: Gradient Versus Isocratic; 2.1.3 Vendor Selection; 2.1.4 Brand Names and Clones; 2.1.5 Hardware-Service-Support; 2.2 System Cost Estimates; 2.2.1 Type I System-QC Isocratic (Cost: 10-15,000); 2.2.2 Type II System-Research Gradient (Cost: 20-25,000); 2.2.3 Type III System-Automated Clinical (Cost: 25-35,000); 2.2.4 Type IV System-Automated Methods (Cost: 30-50,000); 2.3 Columns; 2.3.1 Sizes: Analytical and Preparative; 2.3.2 Separating Modes: Selecting Only What You Need 2.3.3 Tips on Column Use3 Running Your Chromatograph; 3.1 Set-up and Start-up; 3.1.1 Hardware Plumbing 101: Tubing and Fittings; 3.1.2 Connecting Components; 3.1.3 Solvent Clean-up; 3.1.4 Water Purity Test; 3.1.5 Start-up System Flushing; 3.1.6 Column Preparation and Equilibration; 3.2 Sample Preparation and Column Calibration; 3.2.1 Sample Clean-up; 3.2.2 Plate Counts; 3.3 Your First Chromatogram; 3.3.1 Reproducible Injection Techniques; 3.3.2 Simple Scouting for a Mobile Phase; 3.3.3 Examining the Chromatogram; 3.3.4 Basic Calculations of Results; II HPLC OPTIMIZATION; 4 Separation Models 4.1 Partition4.1.1 Separation Parameters; 4.1.2 Efficiency Factor; 4.1.3 Separation (Chemistry) Factor; 4.2 Ion Exchange Chromatography; 4.3 Size Exclusion Chromatography; 4.4 Affinity Chromatography; 5 Column Preparation; 5.1 Column Variations; 5.2 Packing Materials and Hardware; 5.3 Column Selection; 6 Column Aging, Diagnosis, and Healing; 6.1 Packing Degrading-Bonded-Phase Loss; 6.2 Dissolved Packing Material-End Voids; 6.3 Bound Material; 6.4 Pressure Increases; 6.5 Column Channeling-Center-Voids; 6.6 Normal Phase, Ion Exchange, and Size Columns; 6.7 Zirconium and Polymer Columns 7 Partition Chromatography Modifications7.1 Reverse-Phase and Hybrid Silica; 7.1.1 Ionization Suppression; 7.1.2 Ion Pairing; 7.1.3 Organic Modifiers; 7.1.4 Chelation; 7.2 Acidic Phase Silica; 7.3 Reverse-Phase Zirconium; 7.4 Partition Mode Selection; 8 "Nonpartition" Chromatography; 8.1 Ion Exchange; 8.1.1 Cationic:Weak and Strong; 8.1.2 Anionic:Weak and Strong; 8.2 Size Exclusion; 8.2.1 Organic Soluble Samples; 8.2.2 Hydrophilic Protein Separation; 8.3 Affinity Chromatography; 8.3.1 Column Packing Modification; 8.3.2 Chelation and Optically Active Columns; 9 Hardware Specifics 9.1 System Protection |
| Record Nr. | UNINA-9910141139203321 |
|
McMaster Marvin C
|
||
| Hoboken, N.J., : Wiley-Interscience, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
HPLC, a practical user's guide [[electronic resource] /] / Marvin C. McMaster
| HPLC, a practical user's guide [[electronic resource] /] / Marvin C. McMaster |
| Autore | McMaster Marvin C |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Interscience, c2007 |
| Descrizione fisica | 1 online resource (254 p.) |
| Disciplina |
543.84
543/.84 615.1901 |
| Soggetto topico | High performance liquid chromatography |
| ISBN |
1-280-72157-X
9786610721573 0-470-07908-8 0-470-07909-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
HPLC; CONTENTS; PREFACE; I HPLC PRIMER; 1 Advantages and Disadvantages of HPLC; 1.1 How It Works; 1.1.1 A Separation Model of the Column; 1.1.2 Basic Hardware: A Quick, First Look; 1.1.3 Use of Solvent Gradients; 1.1.4 Ranges of Compounds; 1.2 Other Ways to Make My Separation; 1.2.1 FPLC-Fast Protein Liquid Chromatography; 1.2.2 LC-Traditional Liquid Chromatography; 1.2.3 GLC-Gas Liquid Chromatography; 1.2.4 SFC-Supercritical Fluid Chromatography; 1.2.5 TLC-Thin Layer Chromatography; 1.2.6 EP-Electrophoresis; 1.2.7 CZE-Capillary Zone Electrophoresis; 2 Selecting an HPLC System
2.1 Characteristic Systems2.1.1 Finding a Fit: Detectors and Data Processing; 2.1.2 System Models: Gradient Versus Isocratic; 2.1.3 Vendor Selection; 2.1.4 Brand Names and Clones; 2.1.5 Hardware-Service-Support; 2.2 System Cost Estimates; 2.2.1 Type I System-QC Isocratic (Cost: 10-15,000); 2.2.2 Type II System-Research Gradient (Cost: 20-25,000); 2.2.3 Type III System-Automated Clinical (Cost: 25-35,000); 2.2.4 Type IV System-Automated Methods (Cost: 30-50,000); 2.3 Columns; 2.3.1 Sizes: Analytical and Preparative; 2.3.2 Separating Modes: Selecting Only What You Need 2.3.3 Tips on Column Use3 Running Your Chromatograph; 3.1 Set-up and Start-up; 3.1.1 Hardware Plumbing 101: Tubing and Fittings; 3.1.2 Connecting Components; 3.1.3 Solvent Clean-up; 3.1.4 Water Purity Test; 3.1.5 Start-up System Flushing; 3.1.6 Column Preparation and Equilibration; 3.2 Sample Preparation and Column Calibration; 3.2.1 Sample Clean-up; 3.2.2 Plate Counts; 3.3 Your First Chromatogram; 3.3.1 Reproducible Injection Techniques; 3.3.2 Simple Scouting for a Mobile Phase; 3.3.3 Examining the Chromatogram; 3.3.4 Basic Calculations of Results; II HPLC OPTIMIZATION; 4 Separation Models 4.1 Partition4.1.1 Separation Parameters; 4.1.2 Efficiency Factor; 4.1.3 Separation (Chemistry) Factor; 4.2 Ion Exchange Chromatography; 4.3 Size Exclusion Chromatography; 4.4 Affinity Chromatography; 5 Column Preparation; 5.1 Column Variations; 5.2 Packing Materials and Hardware; 5.3 Column Selection; 6 Column Aging, Diagnosis, and Healing; 6.1 Packing Degrading-Bonded-Phase Loss; 6.2 Dissolved Packing Material-End Voids; 6.3 Bound Material; 6.4 Pressure Increases; 6.5 Column Channeling-Center-Voids; 6.6 Normal Phase, Ion Exchange, and Size Columns; 6.7 Zirconium and Polymer Columns 7 Partition Chromatography Modifications7.1 Reverse-Phase and Hybrid Silica; 7.1.1 Ionization Suppression; 7.1.2 Ion Pairing; 7.1.3 Organic Modifiers; 7.1.4 Chelation; 7.2 Acidic Phase Silica; 7.3 Reverse-Phase Zirconium; 7.4 Partition Mode Selection; 8 "Nonpartition" Chromatography; 8.1 Ion Exchange; 8.1.1 Cationic:Weak and Strong; 8.1.2 Anionic:Weak and Strong; 8.2 Size Exclusion; 8.2.1 Organic Soluble Samples; 8.2.2 Hydrophilic Protein Separation; 8.3 Affinity Chromatography; 8.3.1 Column Packing Modification; 8.3.2 Chelation and Optically Active Columns; 9 Hardware Specifics 9.1 System Protection |
| Record Nr. | UNINA-9910830870903321 |
|
McMaster Marvin C
|
||
| Hoboken, N.J., : Wiley-Interscience, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
HPLC, a practical user's guide [[electronic resource] /] / Marvin C. McMaster
| HPLC, a practical user's guide [[electronic resource] /] / Marvin C. McMaster |
| Autore | McMaster Marvin C |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Interscience, c2007 |
| Descrizione fisica | 1 online resource (254 p.) |
| Disciplina |
543.84
543/.84 615.1901 |
| Soggetto topico | High performance liquid chromatography |
| ISBN |
1-280-72157-X
9786610721573 0-470-07908-8 0-470-07909-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
HPLC; CONTENTS; PREFACE; I HPLC PRIMER; 1 Advantages and Disadvantages of HPLC; 1.1 How It Works; 1.1.1 A Separation Model of the Column; 1.1.2 Basic Hardware: A Quick, First Look; 1.1.3 Use of Solvent Gradients; 1.1.4 Ranges of Compounds; 1.2 Other Ways to Make My Separation; 1.2.1 FPLC-Fast Protein Liquid Chromatography; 1.2.2 LC-Traditional Liquid Chromatography; 1.2.3 GLC-Gas Liquid Chromatography; 1.2.4 SFC-Supercritical Fluid Chromatography; 1.2.5 TLC-Thin Layer Chromatography; 1.2.6 EP-Electrophoresis; 1.2.7 CZE-Capillary Zone Electrophoresis; 2 Selecting an HPLC System
2.1 Characteristic Systems2.1.1 Finding a Fit: Detectors and Data Processing; 2.1.2 System Models: Gradient Versus Isocratic; 2.1.3 Vendor Selection; 2.1.4 Brand Names and Clones; 2.1.5 Hardware-Service-Support; 2.2 System Cost Estimates; 2.2.1 Type I System-QC Isocratic (Cost: 10-15,000); 2.2.2 Type II System-Research Gradient (Cost: 20-25,000); 2.2.3 Type III System-Automated Clinical (Cost: 25-35,000); 2.2.4 Type IV System-Automated Methods (Cost: 30-50,000); 2.3 Columns; 2.3.1 Sizes: Analytical and Preparative; 2.3.2 Separating Modes: Selecting Only What You Need 2.3.3 Tips on Column Use3 Running Your Chromatograph; 3.1 Set-up and Start-up; 3.1.1 Hardware Plumbing 101: Tubing and Fittings; 3.1.2 Connecting Components; 3.1.3 Solvent Clean-up; 3.1.4 Water Purity Test; 3.1.5 Start-up System Flushing; 3.1.6 Column Preparation and Equilibration; 3.2 Sample Preparation and Column Calibration; 3.2.1 Sample Clean-up; 3.2.2 Plate Counts; 3.3 Your First Chromatogram; 3.3.1 Reproducible Injection Techniques; 3.3.2 Simple Scouting for a Mobile Phase; 3.3.3 Examining the Chromatogram; 3.3.4 Basic Calculations of Results; II HPLC OPTIMIZATION; 4 Separation Models 4.1 Partition4.1.1 Separation Parameters; 4.1.2 Efficiency Factor; 4.1.3 Separation (Chemistry) Factor; 4.2 Ion Exchange Chromatography; 4.3 Size Exclusion Chromatography; 4.4 Affinity Chromatography; 5 Column Preparation; 5.1 Column Variations; 5.2 Packing Materials and Hardware; 5.3 Column Selection; 6 Column Aging, Diagnosis, and Healing; 6.1 Packing Degrading-Bonded-Phase Loss; 6.2 Dissolved Packing Material-End Voids; 6.3 Bound Material; 6.4 Pressure Increases; 6.5 Column Channeling-Center-Voids; 6.6 Normal Phase, Ion Exchange, and Size Columns; 6.7 Zirconium and Polymer Columns 7 Partition Chromatography Modifications7.1 Reverse-Phase and Hybrid Silica; 7.1.1 Ionization Suppression; 7.1.2 Ion Pairing; 7.1.3 Organic Modifiers; 7.1.4 Chelation; 7.2 Acidic Phase Silica; 7.3 Reverse-Phase Zirconium; 7.4 Partition Mode Selection; 8 "Nonpartition" Chromatography; 8.1 Ion Exchange; 8.1.1 Cationic:Weak and Strong; 8.1.2 Anionic:Weak and Strong; 8.2 Size Exclusion; 8.2.1 Organic Soluble Samples; 8.2.2 Hydrophilic Protein Separation; 8.3 Affinity Chromatography; 8.3.1 Column Packing Modification; 8.3.2 Chelation and Optically Active Columns; 9 Hardware Specifics 9.1 System Protection |
| Record Nr. | UNINA-9910841384403321 |
|
McMaster Marvin C
|
||
| Hoboken, N.J., : Wiley-Interscience, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
LC/MS : a practical user's guide / / Marvin C. McMaster
| LC/MS : a practical user's guide / / Marvin C. McMaster |
| Autore | McMaster Marvin C |
| Pubbl/distr/stampa | Hoboken, N.J., : John Wiley, c2005 |
| Descrizione fisica | 1 online resource (179 p.) |
| Disciplina |
543.84
543/.84 |
| Soggetto topico |
Liquid chromatography
High performance liquid chromatography Mass spectrometry |
| ISBN |
1-280-27734-3
9786610277346 0-471-73658-9 0-471-73657-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
LC/MS; CONTENTS; Preface; 1 Introduction to LC/MS; 1.1 Why LC/MS?; 1.2 Molecular Weights and Structure Studies; 1.3 LC/MS Systems; 1.4 System Costs; 1.5 Competitive Systems; 2 The HPLC System; 2.1 HPLC System Components; 2.2 Gradient versus Isocratic Systems; 2.3 Micro HPLC Systems; 2.4 HPLC Tubing and Fittings; 3 The HPLC Column and Separation Modes; 3.1 Column Construction; 3.2 Column Packing Materials; 3.3 Normal-Phase Columns; 3.4 Other Bonded-Phase Silica Columns; 3.5 Optimizing Reverse-Phase Column Use; 3.6 Silica Ion-Exchange Columns; 3.7 Silica Size-Separation Columns
3.8 Zirconium Bonded-Phase Columns3.9 Polymer Reverse-Phase Columns; 4 HPLC and Column Maintenance; 4.1 HPLC Maintenance; 4.2 Column Maintenance; 5 Sample Preparation and Separations Development; 5.1 Mobile-Phase Preparation; 5.2 Mobile-Phase pH Control Using Buffers; 5.3 Sample Preparation; 5.4 Cartridge Column Cleanup; 5.5 On-Column Sample Concentration; 5.6 Isocratic and Gradient Methods Development; 5.7 Automated Methods Development; 6 LC/MS Interfaces; 6.1 Solvent Removal and Ionization; 6.2 Atmospheric-Pressure Interfaces; 6.3 Electrospray Interface; 6.4 Ion Spray Interface 6.5 Secondary Detectors7 LC/MS Overview; 7.1 HPLC and the Ionization Source; 7.2 Vacuum Pumps; 7.3 Analyzer and Ion Detector Designs; 7.4 Data and Control Systems; 7.5 Peak Detection, ID, and Quantitation; 8 Mass Analyzers; 8.1 Quadrupole Analyzer; 8.2 Ion Trap Analyzer; 8.3 Linear Ion Trap Analyzer; 8.4 Time-of-Flight Analyzer; 8.5 Fourier Transform Analyzer Design; 8.6 Magnetic Sector Analyzers; 9 Mass Spectrometer Maintenance; 9.1 High-Vacuum Operation; 9.2 MS Hardware Maintenance; 9.3 System Electrical Grounding; 10 Application Areas for LC/MS; 10.1 Compound Discovery 10.2 Identification of Complex Biological Compounds10.3 Analysis of Trace Impurities and Metabolites; 10.4 Arson Residue Investigation; 10.5 Industrial Water and Pesticide Analysis; 10.6 Toxicology and Drugs of Abuse; 10.7 Clinical Therapeutic Drug Screening; 10.8 Pesticide Manufacturing; 11 Trace Analysis and LC/MS/MS; 11.1 LC/MS/MS Triple-Quadrupole System; 11.2 MS/MS Operating Modes; 11.3 Ion Trap MS/MS Operation; 11.4 Hybrid LC/MS/MS Systems; 12 Drug Discovery and Benchtop LC/MS; 12.1 Activity Screening; 12.2 Standardized LC/MS Screening 12.3 Molecular Fragmentation for Structural Determination12.4 Process Monitoring; 13 Proteomics: LC/MALDI/TOF and MS/MS Libraries; 13.1 Protein Molecular-Weight Determination by LC/MS; 13.2 De Novo Protein Purification; 13.3 Protein Analysis by Two-Dimensional GEP and LC/TOFMS; 13.4 LC/MS/MS Identification of Peptide Structures; 13.5 Tracer Labeling for Peptide ID; 13.6 Posttranslational Modified Protein; 13.7 Transient Peptides and Accumulation Proteins; 14 The Future of LC/MS; 14.1 Instrumentation Improvements; 14.2 Affordable Benchtop LC/LITMS; 14.3 User-Customized Data Libraries 14.4 Nucleomics and Restriction Fragment Analysis |
| Record Nr. | UNINA-9910143569103321 |
|
McMaster Marvin C
|
||
| Hoboken, N.J., : John Wiley, c2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
LC/MS : a practical user's guide / / Marvin C. McMaster
| LC/MS : a practical user's guide / / Marvin C. McMaster |
| Autore | McMaster Marvin C |
| Pubbl/distr/stampa | Hoboken, N.J., : John Wiley, c2005 |
| Descrizione fisica | 1 online resource (179 p.) |
| Disciplina |
543.84
543/.84 |
| Soggetto topico |
Liquid chromatography
High performance liquid chromatography Mass spectrometry |
| ISBN |
1-280-27734-3
9786610277346 0-471-73658-9 0-471-73657-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
LC/MS; CONTENTS; Preface; 1 Introduction to LC/MS; 1.1 Why LC/MS?; 1.2 Molecular Weights and Structure Studies; 1.3 LC/MS Systems; 1.4 System Costs; 1.5 Competitive Systems; 2 The HPLC System; 2.1 HPLC System Components; 2.2 Gradient versus Isocratic Systems; 2.3 Micro HPLC Systems; 2.4 HPLC Tubing and Fittings; 3 The HPLC Column and Separation Modes; 3.1 Column Construction; 3.2 Column Packing Materials; 3.3 Normal-Phase Columns; 3.4 Other Bonded-Phase Silica Columns; 3.5 Optimizing Reverse-Phase Column Use; 3.6 Silica Ion-Exchange Columns; 3.7 Silica Size-Separation Columns
3.8 Zirconium Bonded-Phase Columns3.9 Polymer Reverse-Phase Columns; 4 HPLC and Column Maintenance; 4.1 HPLC Maintenance; 4.2 Column Maintenance; 5 Sample Preparation and Separations Development; 5.1 Mobile-Phase Preparation; 5.2 Mobile-Phase pH Control Using Buffers; 5.3 Sample Preparation; 5.4 Cartridge Column Cleanup; 5.5 On-Column Sample Concentration; 5.6 Isocratic and Gradient Methods Development; 5.7 Automated Methods Development; 6 LC/MS Interfaces; 6.1 Solvent Removal and Ionization; 6.2 Atmospheric-Pressure Interfaces; 6.3 Electrospray Interface; 6.4 Ion Spray Interface 6.5 Secondary Detectors7 LC/MS Overview; 7.1 HPLC and the Ionization Source; 7.2 Vacuum Pumps; 7.3 Analyzer and Ion Detector Designs; 7.4 Data and Control Systems; 7.5 Peak Detection, ID, and Quantitation; 8 Mass Analyzers; 8.1 Quadrupole Analyzer; 8.2 Ion Trap Analyzer; 8.3 Linear Ion Trap Analyzer; 8.4 Time-of-Flight Analyzer; 8.5 Fourier Transform Analyzer Design; 8.6 Magnetic Sector Analyzers; 9 Mass Spectrometer Maintenance; 9.1 High-Vacuum Operation; 9.2 MS Hardware Maintenance; 9.3 System Electrical Grounding; 10 Application Areas for LC/MS; 10.1 Compound Discovery 10.2 Identification of Complex Biological Compounds10.3 Analysis of Trace Impurities and Metabolites; 10.4 Arson Residue Investigation; 10.5 Industrial Water and Pesticide Analysis; 10.6 Toxicology and Drugs of Abuse; 10.7 Clinical Therapeutic Drug Screening; 10.8 Pesticide Manufacturing; 11 Trace Analysis and LC/MS/MS; 11.1 LC/MS/MS Triple-Quadrupole System; 11.2 MS/MS Operating Modes; 11.3 Ion Trap MS/MS Operation; 11.4 Hybrid LC/MS/MS Systems; 12 Drug Discovery and Benchtop LC/MS; 12.1 Activity Screening; 12.2 Standardized LC/MS Screening 12.3 Molecular Fragmentation for Structural Determination12.4 Process Monitoring; 13 Proteomics: LC/MALDI/TOF and MS/MS Libraries; 13.1 Protein Molecular-Weight Determination by LC/MS; 13.2 De Novo Protein Purification; 13.3 Protein Analysis by Two-Dimensional GEP and LC/TOFMS; 13.4 LC/MS/MS Identification of Peptide Structures; 13.5 Tracer Labeling for Peptide ID; 13.6 Posttranslational Modified Protein; 13.7 Transient Peptides and Accumulation Proteins; 14 The Future of LC/MS; 14.1 Instrumentation Improvements; 14.2 Affordable Benchtop LC/LITMS; 14.3 User-Customized Data Libraries 14.4 Nucleomics and Restriction Fragment Analysis |
| Record Nr. | UNINA-9910830933703321 |
|
McMaster Marvin C
|
||
| Hoboken, N.J., : John Wiley, c2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
LC/MS : a practical user's guide / / Marvin C. McMaster
| LC/MS : a practical user's guide / / Marvin C. McMaster |
| Autore | McMaster Marvin C |
| Pubbl/distr/stampa | Hoboken, N.J., : John Wiley, c2005 |
| Descrizione fisica | 1 online resource (179 p.) |
| Disciplina |
543.84
543/.84 |
| Soggetto topico |
Liquid chromatography
High performance liquid chromatography Mass spectrometry |
| ISBN |
1-280-27734-3
9786610277346 0-471-73658-9 0-471-73657-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
LC/MS; CONTENTS; Preface; 1 Introduction to LC/MS; 1.1 Why LC/MS?; 1.2 Molecular Weights and Structure Studies; 1.3 LC/MS Systems; 1.4 System Costs; 1.5 Competitive Systems; 2 The HPLC System; 2.1 HPLC System Components; 2.2 Gradient versus Isocratic Systems; 2.3 Micro HPLC Systems; 2.4 HPLC Tubing and Fittings; 3 The HPLC Column and Separation Modes; 3.1 Column Construction; 3.2 Column Packing Materials; 3.3 Normal-Phase Columns; 3.4 Other Bonded-Phase Silica Columns; 3.5 Optimizing Reverse-Phase Column Use; 3.6 Silica Ion-Exchange Columns; 3.7 Silica Size-Separation Columns
3.8 Zirconium Bonded-Phase Columns3.9 Polymer Reverse-Phase Columns; 4 HPLC and Column Maintenance; 4.1 HPLC Maintenance; 4.2 Column Maintenance; 5 Sample Preparation and Separations Development; 5.1 Mobile-Phase Preparation; 5.2 Mobile-Phase pH Control Using Buffers; 5.3 Sample Preparation; 5.4 Cartridge Column Cleanup; 5.5 On-Column Sample Concentration; 5.6 Isocratic and Gradient Methods Development; 5.7 Automated Methods Development; 6 LC/MS Interfaces; 6.1 Solvent Removal and Ionization; 6.2 Atmospheric-Pressure Interfaces; 6.3 Electrospray Interface; 6.4 Ion Spray Interface 6.5 Secondary Detectors7 LC/MS Overview; 7.1 HPLC and the Ionization Source; 7.2 Vacuum Pumps; 7.3 Analyzer and Ion Detector Designs; 7.4 Data and Control Systems; 7.5 Peak Detection, ID, and Quantitation; 8 Mass Analyzers; 8.1 Quadrupole Analyzer; 8.2 Ion Trap Analyzer; 8.3 Linear Ion Trap Analyzer; 8.4 Time-of-Flight Analyzer; 8.5 Fourier Transform Analyzer Design; 8.6 Magnetic Sector Analyzers; 9 Mass Spectrometer Maintenance; 9.1 High-Vacuum Operation; 9.2 MS Hardware Maintenance; 9.3 System Electrical Grounding; 10 Application Areas for LC/MS; 10.1 Compound Discovery 10.2 Identification of Complex Biological Compounds10.3 Analysis of Trace Impurities and Metabolites; 10.4 Arson Residue Investigation; 10.5 Industrial Water and Pesticide Analysis; 10.6 Toxicology and Drugs of Abuse; 10.7 Clinical Therapeutic Drug Screening; 10.8 Pesticide Manufacturing; 11 Trace Analysis and LC/MS/MS; 11.1 LC/MS/MS Triple-Quadrupole System; 11.2 MS/MS Operating Modes; 11.3 Ion Trap MS/MS Operation; 11.4 Hybrid LC/MS/MS Systems; 12 Drug Discovery and Benchtop LC/MS; 12.1 Activity Screening; 12.2 Standardized LC/MS Screening 12.3 Molecular Fragmentation for Structural Determination12.4 Process Monitoring; 13 Proteomics: LC/MALDI/TOF and MS/MS Libraries; 13.1 Protein Molecular-Weight Determination by LC/MS; 13.2 De Novo Protein Purification; 13.3 Protein Analysis by Two-Dimensional GEP and LC/TOFMS; 13.4 LC/MS/MS Identification of Peptide Structures; 13.5 Tracer Labeling for Peptide ID; 13.6 Posttranslational Modified Protein; 13.7 Transient Peptides and Accumulation Proteins; 14 The Future of LC/MS; 14.1 Instrumentation Improvements; 14.2 Affordable Benchtop LC/LITMS; 14.3 User-Customized Data Libraries 14.4 Nucleomics and Restriction Fragment Analysis |
| Record Nr. | UNINA-9910841128603321 |
|
McMaster Marvin C
|
||
| Hoboken, N.J., : John Wiley, c2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||