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Methods in bioengineering : alternatives to animal testing / / Tim Maguire, Eric Novik, editors
| Methods in bioengineering : alternatives to animal testing / / Tim Maguire, Eric Novik, editors |
| Pubbl/distr/stampa | Boston, Massachusetts : , : Artech House, , ©2010 |
| Descrizione fisica | 1 online resource (298 p.) |
| Disciplina | 179.4 |
| Altri autori (Persone) |
MaguireTimothy J (Timothy John)
NovikEric |
| Collana | Methods in bioengineering series |
| Soggetto topico |
Animal experimentation - Moral and ethical aspects
Animal welfare - Moral and ethical aspects Human-animal relationships - Moral and ethical aspects |
| Soggetto genere / forma | Electronic books. |
| ISBN | 1-60807-012-3 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Contents; Preface; Chapter 1 Current Methods for Prediction of Human Hepatic Clearance Using In Vitro Intrinsic Clearance; 1.1 Introduction; 1.2 Materials; 1.3 Methods; 1.3.1 Thawing the hepatocytes; 1.3.2 Clearance study using a hepatocyte suspension; 1.3.3 Clearance study using a plated hepatocyte culture; 1.3.4 Clearance study using a plated hepatocyte culture under a flow condition; 1.3.5 Sampling for the clearance study; 1.3.6 Sample analysis using LC-MS/MS; 1.4 Data Acquisition, Anticipated Results, and Interpretation; 1.4.1 Hepatocyte suspension and plated hepatocyte system
1.4.2 Physiologically based microfluidic systems1.5 Discussion and Commentary; 1.5.1 Hepatocyte suspension system; 1.5.2 Plated hepatocyte system; 1.5.3 Physiologically based microfluidic systems; 1.6 Summary; References; Chapter 2 Use of Permeability from Cultured Cell Linesand PAMPA System and Absorption from Experimental Animals for the Prediction of Absorption in Humans; 2.1 Introduction; 2.2 Materials; 2.3 Methods; 2.3.1 Cultured cell system; 2.3.2 PAMPA system; 2.3.3 In vivo absorption measurements; 2.4 Data Acquisition, Anticipated Results, and Interpretation; 2.4.1 Data analysis 2.4.2 Results and interpretation2.5 Discussion and Commentary; 2.5.1 Cell culture and PAMPA systems; 2.5.2 Absorption in experimental animals; 2.5.3 Rats; 2.5.4 Dogs; 2.5.5 Monkeys; 2.6 Summary; References; Chapter 3 Aggregating Brain Cell Cultures for Neurotoxicity Tests; 3.1 Introduction; 3.2 Experimental Design; 3.3 Materials; 3.3.1 Animals; 3.3.2 Special equipment; 3.3.3 Reagents; 3.3.4 Preparation of solutions and media; 3.4 Methods; 3.4.1 Washing and sterilizing the glassware; 3.4.2 Cell isolation and culture preparation 3.4.3 Maintenance of aggregating brain cell cultures (media replenishmentand subdivision)3.4.4 Preparation and treatment of replicate cultures; 3.4.5 Harvest of replicate cultures for various analytical procedures; 3.4.6 Examples of sample preparation and use for various analytical procedures; 3.4.7 Data Analysis; 3.5 Anticipated Results; 3.6 Discussion and Commentary; 3.7 Application Notes; 3.8 Summary Points; Acknowledgments; References; Chapter 4 Approaches Towards a Multiscale Model of Systemic Inflammation in Humans; 4.1 Introduction; 4.2 Materials 4.2.1 Human endotoxin model and data collection4.3 Methods; 4.3.1 Transcriptional dynamics and intrinsic responses; 4.3.2 Modeling inflammation at the cellular level; 4.3.3 Modeling inflammation at the systemic level; 4.4 Results; 4.4.1 Elements of the multiscale host response model of human inflammation; 4.4.2 Estimation of relevant model parameters; 4.4.3 Qualitative assessment of the model; 4.5 Conclusions; Acknowledgments; References; Chapter 5 A Liposome Assay for Evaluating the Ocular Toxicity of Chemicals; 5.1 Introduction; 5.2 Experimental Design; 5.3 Materials; 5.4 Methods 5.4.1 Preparation of calcein-loaded liposomes |
| Record Nr. | UNINA-9910459222003321 |
| Boston, Massachusetts : , : Artech House, , ©2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Methods in bioengineering : alternatives to animal testing / / Tim Maguire, Eric Novik, editors
| Methods in bioengineering : alternatives to animal testing / / Tim Maguire, Eric Novik, editors |
| Pubbl/distr/stampa | Boston, Massachusetts : , : Artech House, , ©2010 |
| Descrizione fisica | 1 online resource (298 p.) |
| Disciplina | 179.4 |
| Altri autori (Persone) |
MaguireTimothy J (Timothy John)
NovikEric |
| Collana | Methods in bioengineering series |
| Soggetto topico |
Animal experimentation - Moral and ethical aspects
Animal welfare - Moral and ethical aspects Human-animal relationships - Moral and ethical aspects |
| ISBN |
1-5231-4630-3
1-60807-012-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Contents; Preface; Chapter 1 Current Methods for Prediction of Human Hepatic Clearance Using In Vitro Intrinsic Clearance; 1.1 Introduction; 1.2 Materials; 1.3 Methods; 1.3.1 Thawing the hepatocytes; 1.3.2 Clearance study using a hepatocyte suspension; 1.3.3 Clearance study using a plated hepatocyte culture; 1.3.4 Clearance study using a plated hepatocyte culture under a flow condition; 1.3.5 Sampling for the clearance study; 1.3.6 Sample analysis using LC-MS/MS; 1.4 Data Acquisition, Anticipated Results, and Interpretation; 1.4.1 Hepatocyte suspension and plated hepatocyte system
1.4.2 Physiologically based microfluidic systems1.5 Discussion and Commentary; 1.5.1 Hepatocyte suspension system; 1.5.2 Plated hepatocyte system; 1.5.3 Physiologically based microfluidic systems; 1.6 Summary; References; Chapter 2 Use of Permeability from Cultured Cell Linesand PAMPA System and Absorption from Experimental Animals for the Prediction of Absorption in Humans; 2.1 Introduction; 2.2 Materials; 2.3 Methods; 2.3.1 Cultured cell system; 2.3.2 PAMPA system; 2.3.3 In vivo absorption measurements; 2.4 Data Acquisition, Anticipated Results, and Interpretation; 2.4.1 Data analysis 2.4.2 Results and interpretation2.5 Discussion and Commentary; 2.5.1 Cell culture and PAMPA systems; 2.5.2 Absorption in experimental animals; 2.5.3 Rats; 2.5.4 Dogs; 2.5.5 Monkeys; 2.6 Summary; References; Chapter 3 Aggregating Brain Cell Cultures for Neurotoxicity Tests; 3.1 Introduction; 3.2 Experimental Design; 3.3 Materials; 3.3.1 Animals; 3.3.2 Special equipment; 3.3.3 Reagents; 3.3.4 Preparation of solutions and media; 3.4 Methods; 3.4.1 Washing and sterilizing the glassware; 3.4.2 Cell isolation and culture preparation 3.4.3 Maintenance of aggregating brain cell cultures (media replenishmentand subdivision)3.4.4 Preparation and treatment of replicate cultures; 3.4.5 Harvest of replicate cultures for various analytical procedures; 3.4.6 Examples of sample preparation and use for various analytical procedures; 3.4.7 Data Analysis; 3.5 Anticipated Results; 3.6 Discussion and Commentary; 3.7 Application Notes; 3.8 Summary Points; Acknowledgments; References; Chapter 4 Approaches Towards a Multiscale Model of Systemic Inflammation in Humans; 4.1 Introduction; 4.2 Materials 4.2.1 Human endotoxin model and data collection4.3 Methods; 4.3.1 Transcriptional dynamics and intrinsic responses; 4.3.2 Modeling inflammation at the cellular level; 4.3.3 Modeling inflammation at the systemic level; 4.4 Results; 4.4.1 Elements of the multiscale host response model of human inflammation; 4.4.2 Estimation of relevant model parameters; 4.4.3 Qualitative assessment of the model; 4.5 Conclusions; Acknowledgments; References; Chapter 5 A Liposome Assay for Evaluating the Ocular Toxicity of Chemicals; 5.1 Introduction; 5.2 Experimental Design; 5.3 Materials; 5.4 Methods 5.4.1 Preparation of calcein-loaded liposomes |
| Record Nr. | UNINA-9910785149403321 |
| Boston, Massachusetts : , : Artech House, , ©2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||