Dried blood spots : applications and techniques / / edited by Wenkui Li, Mike S. Lee |
Pubbl/distr/stampa | Hoboken, New Jersey : , : Wiley, , 2014 |
Descrizione fisica | 1 online resource (377 p.) |
Disciplina | 616.07/561 |
Collana | Wiley Series on Pharmaceutical Science and Biotechnology : Practices, Applications and Methods |
Soggetto topico |
Blood - Collection and preservation
Phlebotomy Forensic hematology Forensic serology |
ISBN |
1-118-89089-2
1-118-89083-3 1-118-89090-6 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Dried Blood Spots; Contents; Preface; Contributors; PART I History, Applications, and Healthcare; 1 Overview of the History and Applications of Dried Blood Samples; 1.1 History; 1.2 Historical Applications; 1.3 Overall Advantages and Disadvantages of Dried-Blood Spot Sampling; 1.3.1 Advantages; 1.3.2 Disadvantages; 1.4 Conclusion; References; 2 Dried Blood Spot Cards; 2.1 Introduction; 2.2 Filter Paper; 2.3 Filter Paper Sources; 2.4 Guidelines and Printing; 2.5 Printing Adaptations; 2.6 Further Innovations in Manufacturing; 2.7 Filter Paper Cards Construction
2.8 Wraparound Cover and Cassette Construction 2.9 Filter Paper Pre-Treatment; 2.10 The Future of the Filter Paper Card; References; 3 Dried Blood Spot Sample Collection, Storage, and Transportation; 3.1 Introduction; 3.2 General Procedures and Precautions for DBS Sample Collection; 3.2.1 Reagents and Materials; 3.2.2 Biosafety and Infection Control; 3.2.3 Specimen Collection; 3.2.4 DBS Prepared by Other Collection Methods; 3.2.5 Labeling of DBS; 3.2.6 Drying; 3.3 Sample Storage and Transportation; 3.3.1 Packaging and Storage; 3.3.2 Transportation 3.4 Common Errors in Sample Collection, Storage, and Shipment 3.5 Conclusions; Disclaimer; References; 4 Dried Blood Spot Specimens for Polymerase Chain Reaction in Molecular Diagnostics and Public Health Surveillance; 4.1 Introduction; 4.2 Pioneering DBS Nucleic Acid Extraction Methods for PCR; 4.3 Direct PCR Amplification of Targeted Genomic DNA from DBS; 4.4 Expansion of DBS-PCR from Newborn Screening to Diagnosis of Infectious and other Diseases; 4.5 Application of DBS-PCR in Sequencing-Based Molecular Epidemiology Analyses 4.6 Application of DBS-PCR for HIV Drug Resistance Surveillance and Monitoring in Resource-Limited Settings4.7 Quantitation of Infectious Agent Particles with DBS-PCR; 4.8 Conclusions; References; 5 Application of Enzyme Immunoassay Methods Using Dried Blood Spot Specimens; 5.1 Introduction; 5.2 Overview of Basic ELISA Systems; 5.2.1 Antibody Detection; 5.2.2 Variation of ELISA Method for Antibody Detection; 5.2.3 Variations of ELISA for Antigen Detection; 5.3 Applications of ELISA Methods Using DBS; 5.4 Optimization and ELISA Methods Validation Using DBS Specimens; 5.4.1 DBS Elution Protocol 5.4.2 Defining Cutoff Values5.5 Factors Influencing the Performance of DBS-based ELISA Methods; 5.5.1 DBS Preparation; 5.5.2 Anticoagulant; 5.5.3 Punching; 5.5.4 Cross-Contamination; 5.6 Conclusions; Disclaimer; References; 6 Applications of Dried Blood Spots in Newborn and Metabolic Screening; 6.1 Introduction; 6.1.1 History and Chronology in the Use of DBS in NBS; 6.1.2 Chapter Organization and Perspectives; 6.2 Fundamentals of Dried Blood Spots; 6.2.1 A Class II Medical Device; 6.2.2 Liquid versus Dried Blood Specimens; 6.2.3 Interaction of Paper with Biological Matrix and Substrates 6.2.4 Sample Collection and DBS |
Record Nr. | UNINA-9910132219103321 |
Hoboken, New Jersey : , : Wiley, , 2014 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Dried blood spots : applications and techniques / / edited by Wenkui Li, Mike S. Lee |
Pubbl/distr/stampa | Hoboken, New Jersey : , : Wiley, , 2014 |
Descrizione fisica | 1 online resource (377 p.) |
Disciplina | 616.07/561 |
Collana | Wiley Series on Pharmaceutical Science and Biotechnology : Practices, Applications and Methods |
Soggetto topico |
Blood - Collection and preservation
Phlebotomy Forensic hematology Forensic serology |
ISBN |
1-118-89089-2
1-118-89083-3 1-118-89090-6 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Dried Blood Spots; Contents; Preface; Contributors; PART I History, Applications, and Healthcare; 1 Overview of the History and Applications of Dried Blood Samples; 1.1 History; 1.2 Historical Applications; 1.3 Overall Advantages and Disadvantages of Dried-Blood Spot Sampling; 1.3.1 Advantages; 1.3.2 Disadvantages; 1.4 Conclusion; References; 2 Dried Blood Spot Cards; 2.1 Introduction; 2.2 Filter Paper; 2.3 Filter Paper Sources; 2.4 Guidelines and Printing; 2.5 Printing Adaptations; 2.6 Further Innovations in Manufacturing; 2.7 Filter Paper Cards Construction
2.8 Wraparound Cover and Cassette Construction 2.9 Filter Paper Pre-Treatment; 2.10 The Future of the Filter Paper Card; References; 3 Dried Blood Spot Sample Collection, Storage, and Transportation; 3.1 Introduction; 3.2 General Procedures and Precautions for DBS Sample Collection; 3.2.1 Reagents and Materials; 3.2.2 Biosafety and Infection Control; 3.2.3 Specimen Collection; 3.2.4 DBS Prepared by Other Collection Methods; 3.2.5 Labeling of DBS; 3.2.6 Drying; 3.3 Sample Storage and Transportation; 3.3.1 Packaging and Storage; 3.3.2 Transportation 3.4 Common Errors in Sample Collection, Storage, and Shipment 3.5 Conclusions; Disclaimer; References; 4 Dried Blood Spot Specimens for Polymerase Chain Reaction in Molecular Diagnostics and Public Health Surveillance; 4.1 Introduction; 4.2 Pioneering DBS Nucleic Acid Extraction Methods for PCR; 4.3 Direct PCR Amplification of Targeted Genomic DNA from DBS; 4.4 Expansion of DBS-PCR from Newborn Screening to Diagnosis of Infectious and other Diseases; 4.5 Application of DBS-PCR in Sequencing-Based Molecular Epidemiology Analyses 4.6 Application of DBS-PCR for HIV Drug Resistance Surveillance and Monitoring in Resource-Limited Settings4.7 Quantitation of Infectious Agent Particles with DBS-PCR; 4.8 Conclusions; References; 5 Application of Enzyme Immunoassay Methods Using Dried Blood Spot Specimens; 5.1 Introduction; 5.2 Overview of Basic ELISA Systems; 5.2.1 Antibody Detection; 5.2.2 Variation of ELISA Method for Antibody Detection; 5.2.3 Variations of ELISA for Antigen Detection; 5.3 Applications of ELISA Methods Using DBS; 5.4 Optimization and ELISA Methods Validation Using DBS Specimens; 5.4.1 DBS Elution Protocol 5.4.2 Defining Cutoff Values5.5 Factors Influencing the Performance of DBS-based ELISA Methods; 5.5.1 DBS Preparation; 5.5.2 Anticoagulant; 5.5.3 Punching; 5.5.4 Cross-Contamination; 5.6 Conclusions; Disclaimer; References; 6 Applications of Dried Blood Spots in Newborn and Metabolic Screening; 6.1 Introduction; 6.1.1 History and Chronology in the Use of DBS in NBS; 6.1.2 Chapter Organization and Perspectives; 6.2 Fundamentals of Dried Blood Spots; 6.2.1 A Class II Medical Device; 6.2.2 Liquid versus Dried Blood Specimens; 6.2.3 Interaction of Paper with Biological Matrix and Substrates 6.2.4 Sample Collection and DBS |
Record Nr. | UNINA-9910823859903321 |
Hoboken, New Jersey : , : Wiley, , 2014 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Handbook of LC-MS bioanalysis [[electronic resource] ] : best practices, experimental protocols, and regulations / / edited by Wenkui Li, Jie Zhang, Francis L.S. Tse |
Pubbl/distr/stampa | Hoboken, N.J., : John Wiley & Sons, 2013 |
Descrizione fisica | 1 online resource (706 p.) |
Disciplina | 615.1/901 |
Altri autori (Persone) |
LiWenkui <1964->
ZhangJie <1962-> TseFrancis L. S |
Soggetto topico |
Drugs - Spectra
Drugs - Analysis Mass spectrometry Liquid chromatography |
ISBN |
1-118-67135-X
1-118-67127-9 1-118-67134-1 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | pt. I. Overview of LC-MS bioanalysis -- pt. II. Current understanding of LC-MS bioanalysis-related regulations -- pt. III. Best practice in LC-MS bioanalysis -- pt. IV. Representative guidelines and/or experimental protocols of LC-MS bioanalysis. |
Record Nr. | UNINA-9910139023203321 |
Hoboken, N.J., : John Wiley & Sons, 2013 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|