Pubbl/distr/stampa	Weinheim, : Wiley-VCH Verlag GmbH & Co. KGaA, 2008
Descrizione fisica	1 online resource (422 p.)
Disciplina	543.0894 543.8 543.84 544.92
Altri autori (Persone)	KromidasStavros KussHans-Joachim
Soggetto topico	Chromatographic analysis High performance liquid chromatography Gas chromatography
Soggetto genere / forma	Electronic books.
ISBN	3-527-66015-1 1-281-94677-X 9786611946777 3-527-62222-5 3-527-62223-3
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	ger
Nota di contenuto	Chromatogramme richtig integrieren und bewerten; Inhaltsverzeichnis; Vorwort; Autorenliste; Zum Aufbau des Buches; Teil I Auswertung in der Chromatographie - die Integration; 1 Das Chromatogramm; 1.1 Chromatographischer Prozess; 1.1.1 Selektivität und Effizienz - Maß für die unterschiedliche Wanderungsgeschwindigkeit; 1.2 Chromatographische Kenngrößen; 1.2.1 Retentionsgrößen; 1.2.1.1 Totzeit (t(m); t(0)); 1.2.1.2 Bruttoretentionszeit (t(ms); t(R)); 1.2.1.3 Nettoretentionszeit (t(s)); 1.2.1.4 Retentionsfaktor oder Kapazitätsfaktor (k; k ́); 1.2.2 Peak-Ausdehnung und Peakform 1.2.2.1 Basispeakbreite (w(b))1.2.2.2 Peakbreite in halber Höhe (w(h)); 1.2.2.3 Peakhöhe (h); 1.2.2.4 Peaksymmetrie, Tailingfaktor (T); 1.2.3 Auflösungsgrößen; 1.2.3.1 Die Auflösung (R); 1.2.3.2 Quantitative Größe der Selektivität; 1.2.3.3 Quantitative Größen für die Effizienz der Trennsäule; 1.2.4 Bestimmung von kleinen Substanzmengen; 1.2.4.1 Ermitteln der Nachweis-, Erfassungs-, Entscheidungs- und Bestimmungsgrenze; 1.3 van Deemter- und Golay-Gleichung; 1.4 Erzeugen von Chromatogrammen; 1.4.1 Datenaufnahme, Erzeugen der Rohdaten; 1.4.1.1 Bei der Datenaufnahme verwendete Parameter 1.4.1.2 Beispiele der unterschiedlichen Art der Datenaufnahme1.4.1.3 Innere/äußere Chromatogramme; 1.4.1.4 2-D-/3-D-Chromatogramme; 1.4.2 Charakterisierung von Detektoren; 1.4.2.1 Zerstörend/nicht zerstörend; 1.4.2.2 Selektiv, spezifisch, universell; 1.4.2.3 Konzentrations- und massenstromabhängige Detektoren; 1.4.2.4 Detektorempfindlichkeit; 1.4.2.5 Linearer und dynamischer Bereich; 1.4.2.6 Ansprechzeit, Zeitkonstante; 1.5 Integration; 1.5.1 Integration anschaulich; 1.5.1.1 Methoden zur Peakerkennung; 1.5.2 Integration und Integrationsparameter, Beispiele 1.5.2.1 Datenaufnahme und -integration mit Empower 21.5.2.2 Datenaufnahme und -integration mit Chromeleon; 1.5.2.3 Datenaufnahme und -integration mit EZChrom Elite; 1.5.2.4 Datenaufnahme und -integration mit ChemStation; 1.5.2.5 Vergleich der wichtigsten Integrationsparameter von vier unterschiedlichen Integrationsprogrammen; Anhang: Experimente zur Optimierung der Zeitkonstante/Datensammelrate; Literatur; 2 Integrationsfehler und Auswertung; 2.1 Was sagt die Literatur über Integrationsfehler?; 2.2 Integration in der täglichen Praxis; 2.2.1 Integration - einfach und immer gleich? 2.2.2 Vergleich von Integrationssystemen mit wenigen großen Peaks2.2.3 Vergleich von Integrationssystemen mit vielen kleinen Peaks; 2.3 Chromatogramm-Simulation; 2.3.1 Simulation eines digitalen Chromatogramms; 2.3.2 Ein Peak; 2.3.3 Mehrere Peaks; 2.3.4 Rauschen; 2.3.5 Drift; 2.3.6 Gaschromatogramm; 2.3.7 Verschmolzene Peaks; 2.3.8 Datenpunktabstand; 2.3.9 Tailing; 2.3.10 Peakfläche und Peakhöhe; 2.3.11 Andere Kenngrößen; 2.4 Anwendungen der Simulation; 2.4.1 Simulation einer Kalibriergeraden; 2.4.2 Zehnfache Simulation an der Bestimmungsgrenze 2.4.3 Simulation eines isokratischen Chromatogramms
Record Nr.	UNINA-9910144117803321

Pubbl/distr/stampa	Weinheim, Germany : , : Wiley-VCH Verlag GmbH & Co. KGaA, , [2019]
Descrizione fisica	1 online resource (252 pages)
Disciplina	543.0894
Soggetto topico	High performance liquid chromatography
Soggetto genere / forma	Electronic books.
ISBN	3-527-81276-8 1-5231-2820-8 3-527-81274-1 3-527-81277-6
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Record Nr.	UNINA-9910555081403321

Pubbl/distr/stampa	Weinheim, : Wiley-VCH
Descrizione fisica	1 online resource (789 p.)
Disciplina	543.0894 543.84
Altri autori (Persone)	KromidasStavros
Soggetto topico	High performance liquid chromatography - Methodology Liquid chromatography
Soggetto genere / forma	Electronic books.
ISBN	1-281-31169-3 9786611311698 3-527-61197-5 3-527-61199-1
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Nota di contenuto	HPLC Made to Measure; Foreword; Preface; Contents; List of Contributors; Structure of the Book; 1 Fundamentals of Optimization; 1.1 Principles of the Optimization of HPLC Illustrated by RP-Chromatography; 1.1.1 Before the First Steps of Optimization; 1.1.2 What Exactly Do We Mean By "Optimization"?; 1.1.3 Improvement of Resolution ("Separate Better"); 1.1.3.1 Principal Possibilities for Improving Resolution; 1.1.3.2 What has the Greatest Effect on Resolution?; 1.1.3.3 Which Sequence of Steps is Most Logical When Attempting an Optimization?; 1.1.3.4 How to Change k, α, and N 1.1.3.4.1 Isocratic Mode1.1.3.4.2 Gradient Mode; 1.1.3.4.3 Acetonitrile or Methanol?; 1.1.4 Testing of the Peak Homogeneity; 1.1.5 Unknown Samples: "How Can I Start?"; Strategies and Concepts; 1.1.5.1 The "Two Days Method"; 1.1.5.2 "The 5-Step Model"; 1.1.6 Shortening of the Run Time ("Faster Separation"); 1.1.7 Improvement of the Sensitivity ("To See More", i.e. Lowering of the Detection Limit); 1.1.8 Economics in HPLC ("Cheaper Separation"); 1.1.9 Final Remarks and Outlook; References; 1.2 Fast Gradient Separations; 1.2.1 Introduction; 1.2.2 Main Part; 1.2.2.1 Theory; 1.2.2.2 Results 1.2.2.2.1 General Relationships1.2.2.2.2 Short Columns, Small Particles; 1.2.2.2.3 An Actual Example; 1.2.2.3 Optimal Operating Conditions and Limits of Currently Available Technology; 1.2.2.4 Problems and Solutions; 1.2.2.4.1 Gradient Delay Volume; 1.2.2.4.2 Detector Sampling Rate and Time Constant; 1.2.2.4.3 Ion Suppression in Mass Spectrometry; References; 1.3 pH and Selectivity in RP-Chromatography; 1.3.1 Introduction; 1.3.2 Main Section; 1.3.2.1 Ionization and pH; 1.3.2.2 Mobile Phase and pH; 1.3.2.2.1 Buffer Capacity 1.3.2.2.2 Changes of pK and pH Value in the Presence of an Organic Solvent1.3.2.3 Buffers; 1.3.2.3.1 Classical HPLC Buffers; 1.3.2.3.2 MS-Compatible pH Control; 1.3.2.4 Influence of the Samples; 1.3.2.4.1 The Sample Type: Acids, Bases, Zwitterions; 1.3.2.4.2 Influence of the Organic Solvent on the Ionization of the Analytes; 1.3.3 Application Example; 1.3.4 Troubleshooting; 1.3.4.1 Reproducibility Problems; 1.3.4.2 Buffer Strength and Solubility; 1.3.4.3 Constant Buffer Concentration; 1.3.5 Summary; References; 1.4 Selecting the Correct pH Value for HPLC; 1.4.1 Introduction 1.4.2 Typical Approaches to pH Selection1.4.3 Initial pH Selection; 1.4.4 Basis of pK(a) Prediction; 1.4.5 Correction of pH Based on Organic Content; 1.4.6 Optimization of Mobile Phase pH Without Chemical Structures; 1.4.7 A Systematic Approach to pH Selection; 1.4.8 An Example - Separation of 1,4-Bis[(2-pyridin-2-ylethyl)thio]butane-2,3-diol from its Impurities; 1.4.9 Troubleshooting Mobile Phase pH; 1.4.10 The Future; 1.4.11 Conclusion; References; 1.5 Optimization of the Evaluation in Chromatography; 1.5.1 Evaluation of Chromatographic Data - An Introduction; 1.5.2 Working Range 1.5.3 Internal Standard
Record Nr.	UNINA-9910146269003321

Pubbl/distr/stampa	Weinheim, Germany : , : Wiley-VCH Verlag GmbH & Company KGaA, , [2016]
Descrizione fisica	1 online resource (411 p.)
Soggetto topico	High performance liquid chromatography
Soggetto genere / forma	Electronic books.
ISBN	3-527-67763-1 3-527-67761-5 3-527-67762-3
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Nota di contenuto	Title Page; Copyright; Table of Contents; List of Contributors; The structure of "The HPLC-Expert"; Preface; Chapter 1: LC/MS Coupling; 1.1 State of the Art in LC/MS; 1.2 Technical Aspects and Pitfalls of LC/MS Hyphenation; 1.3 LC Coupled to MS - A User Report; References; Chapter 2: Optimization Strategies in RP-HPLC; 2.1 Introduction; 2.2 LC Fundamentals; 2.3 Methodology of Optimization; 2.4 Outlook; References; Chapter 3: The Gradient in RP-Chromatography*; 3.1 Aspects of Gradient Optimization; 3.2 Prediction of Gradients; References Chapter 4: Comparison and Selection of Modern HPLC Columns4.1 Supports; 4.2 Stationary Phases for the HPLC: The Historical Development; 4.3 pH Stability and Restrictions in the Use of Silica; 4.4 The Key Properties of Reversed Phases; 4.5 Characterization and Classification of Reversed Phases; 4.6 Procedure for Practical Method Development; 4.7 Column Screening; 4.8 Column Databases; References; Chapter 5: Introduction to Biochromatography; 5.1 Introduction; 5.2 Overview of the Stationary Phases; 5.3 Reversed-Phase Chromatography of Peptides and Proteins 5.4 IEC Chromatography of Peptides and Proteins5.5 Size-Exclusion Chromatography of Peptides and Proteins; 5.6 Further Types of Chromatography - Brief Descriptions; 5.7 Summary; Chapter 6: Comparison of Modern Chromatographic Data Systems; 6.1 Introduction; 6.2 The Forerunners for CDS; 6.3 CDS Today; 6.4 Advantages and Disadvantages of File-Based CDS; 6.5 Advantages and Disadvantages of Database-Supported CDS; 6.6 CDS in a Network Environment; 6.7 Instrument Control; 6.8 Documentation and Compliance; 6.9 Brief Overview of Current Systems; 6.10 The CDS of Tomorrow; 6.11 Special Extensions 6.12 Open Interfaces6.13 The CDS in 20 Years; Acknowledgment; Chapter 7: Possibilities of Integration Today; 7.1 Peak Overlay - Effect on the Chromatogram; 7.2 Separation Techniques for Higher-Level Peaks; 7.3 Application of Separation Methods; 7.4 Chromatogrammsimulation; 7.5 Deconvolution; 7.6 Evaluation of Separation Methods; 7.7 Practical Application of Deconvolution; References; Chapter 8: Smart Documentation Strategies; 8.1 Introduction; 8.2 Objectives of Documentation; 8.3 The Life Cycle Model for Regulated Documents in Practice 8.4 Dealing with Hybrid Systems Comprising Paper and Electronic Records8.5 Preview; References; Chapter 9: Tips for a Successful FDA Inspection; 9.1 Introduction; 9.2 Preparation with the Inspection Model; 9.3 Typical Course of an FDA Inspection; 9.4 During the Inspection; 9.5 Post-Processing of the Inspection; Further Readings; Chapter 10: HPLC - Link List; 10.1 Chemical Data; 10.2 Applications/Methods; 10.3 Troubleshooting; 10.4 Background Information and Theory; 10.5 Literature; 10.6 Databases with Costs; 10.7 Apps; 10.8 Social Media; 10.9 Twitter Pages (Examples) 10.10 Facebook Pages (Examples)
Record Nr.	UNINA-9910136779003321

Pubbl/distr/stampa	Weinheim, Germany : , : Wiley-VCH Verlag GmbH & Company KGaA, , [2016]
Descrizione fisica	1 online resource (411 p.)
Soggetto topico	High performance liquid chromatography
ISBN	3-527-67763-1 3-527-67761-5 3-527-67762-3
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Nota di contenuto	Title Page; Copyright; Table of Contents; List of Contributors; The structure of "The HPLC-Expert"; Preface; Chapter 1: LC/MS Coupling; 1.1 State of the Art in LC/MS; 1.2 Technical Aspects and Pitfalls of LC/MS Hyphenation; 1.3 LC Coupled to MS - A User Report; References; Chapter 2: Optimization Strategies in RP-HPLC; 2.1 Introduction; 2.2 LC Fundamentals; 2.3 Methodology of Optimization; 2.4 Outlook; References; Chapter 3: The Gradient in RP-Chromatography*; 3.1 Aspects of Gradient Optimization; 3.2 Prediction of Gradients; References Chapter 4: Comparison and Selection of Modern HPLC Columns4.1 Supports; 4.2 Stationary Phases for the HPLC: The Historical Development; 4.3 pH Stability and Restrictions in the Use of Silica; 4.4 The Key Properties of Reversed Phases; 4.5 Characterization and Classification of Reversed Phases; 4.6 Procedure for Practical Method Development; 4.7 Column Screening; 4.8 Column Databases; References; Chapter 5: Introduction to Biochromatography; 5.1 Introduction; 5.2 Overview of the Stationary Phases; 5.3 Reversed-Phase Chromatography of Peptides and Proteins 5.4 IEC Chromatography of Peptides and Proteins5.5 Size-Exclusion Chromatography of Peptides and Proteins; 5.6 Further Types of Chromatography - Brief Descriptions; 5.7 Summary; Chapter 6: Comparison of Modern Chromatographic Data Systems; 6.1 Introduction; 6.2 The Forerunners for CDS; 6.3 CDS Today; 6.4 Advantages and Disadvantages of File-Based CDS; 6.5 Advantages and Disadvantages of Database-Supported CDS; 6.6 CDS in a Network Environment; 6.7 Instrument Control; 6.8 Documentation and Compliance; 6.9 Brief Overview of Current Systems; 6.10 The CDS of Tomorrow; 6.11 Special Extensions 6.12 Open Interfaces6.13 The CDS in 20 Years; Acknowledgment; Chapter 7: Possibilities of Integration Today; 7.1 Peak Overlay - Effect on the Chromatogram; 7.2 Separation Techniques for Higher-Level Peaks; 7.3 Application of Separation Methods; 7.4 Chromatogrammsimulation; 7.5 Deconvolution; 7.6 Evaluation of Separation Methods; 7.7 Practical Application of Deconvolution; References; Chapter 8: Smart Documentation Strategies; 8.1 Introduction; 8.2 Objectives of Documentation; 8.3 The Life Cycle Model for Regulated Documents in Practice 8.4 Dealing with Hybrid Systems Comprising Paper and Electronic Records8.5 Preview; References; Chapter 9: Tips for a Successful FDA Inspection; 9.1 Introduction; 9.2 Preparation with the Inspection Model; 9.3 Typical Course of an FDA Inspection; 9.4 During the Inspection; 9.5 Post-Processing of the Inspection; Further Readings; Chapter 10: HPLC - Link List; 10.1 Chemical Data; 10.2 Applications/Methods; 10.3 Troubleshooting; 10.4 Background Information and Theory; 10.5 Literature; 10.6 Databases with Costs; 10.7 Apps; 10.8 Social Media; 10.9 Twitter Pages (Examples) 10.10 Facebook Pages (Examples)
Record Nr.	UNINA-9910830952903321