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Detection of highly dangerous pathogens [[electronic resource] ] : microarray methods for BSL 3 and BSL 4 agents / / edited by Tanja Kostic, Patrick Butaye, and Jacques Schrenzel
| Detection of highly dangerous pathogens [[electronic resource] ] : microarray methods for BSL 3 and BSL 4 agents / / edited by Tanja Kostic, Patrick Butaye, and Jacques Schrenzel |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, 2009 |
| Descrizione fisica | 1 online resource (193 p.) |
| Disciplina | 579.165 |
| Altri autori (Persone) |
KosticTanja
ButayePatrick SchrenzelJacques |
| Soggetto topico |
Pathogenic microorganisms - Detection
DNA microarrays |
| ISBN |
1-282-29205-6
9786612292057 3-527-62668-9 3-527-62669-7 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Detection of Highly Dangerous Pathogens: Microarray Methods for the Detection of BSL 3 and BSL 4 Agents; Contents; This Publication is Supported by COST; Preface; List of Contributors; 1 Introduction to Microarray-Based Detection Methods; 1.1 Introduction to Microarray Technology; 1.2 Technical Aspects of Microarray Technology; 1.2.1 Probes; 1.2.1.1 Genome Fragments; 1.2.1.2 PCR Products; 1.2.1.3 Oligonucleotide Probes; 1.2.2 Substrates for Printing; 1.2.2.1 Slides with Poly-L-lysine Coating; 1.2.2.2 Slides with Amino Silane Coating; 1.2.2.3 Slides with Aldehyde Coating
1.2.2.4 Slides with Epoxy Coating1.2.2.5 Proprietary Surface Chemistries; 1.2.2.6 Probe Spacers; 1.2.3 Targets for Microarray Analysis; 1.2.3.1 Target Amplifications and Sensitivity Issues; 1.2.3.2 Labeling of the Targets; 1.2.3.3 Hybridization and Wash Conditions; 1.2.4 Classical Commercially Available Microarray Formats; 1.2.4.1 Spotting Approaches; 1.2.4.2 In Situ Synthesis; 1.2.5 Alternative Methods for Improving Microarray-Based Detection Sensitivity; 1.2.5.1 Resonance-Light Scattering (RLS); 1.2.5.2 Planar-Waveguide Technology (PWT); 1.2.5.3 Liquid Arrays 1.2.5.4 Three-Dimensional Microarray Formats1.2.6 Marker Genes Used on MDMs; 1.3 Analysis and Quality Control Aspects; 1.4 Applications of Microarray Technology in Microbial Diagnostics; 1.4.1 Gene Expression Studies; 1.4.2 Comparative Genomic Hybridization (CGH); 1.4.3 Generic or Universal Microarrays; 1.4.4 Microarrays for Sequence Analysis; 1.4.5 Microbial Diagnostic Microarrays (MDMs); 1.5 Further Developments and New Perspectives Regarding Array Sensitivity and Specificity; 1.6 Conclusions; References; Part I: Methods; 2 Long Oligonucleotide Microarray-Based Microbial Detection 2.1 Introduction2.2 Method; 2.2.1 DNA Extraction; 2.2.2 Ф29 Amplification; 2.2.3 Klenow Amplification/Labeling; 2.2.4 Probe and Slide Preparation; 2.2.5 Slide Processing Protocol (for Amino Surfaces); 2.2.6 Hybridization and Slide Washing; 2.2.7 Comments; 2.3 Our Test System and Results; 2.4 Conclusions; References; 3 Sequence-Specific End-Labeling of Oligonucleotides; 3.1 Introduction; 3.2 Probe Design; 3.3 Slide Preparation (Spotting); 3.4 Slide Processing Protocol ( for Aldehyde Surfaces); 3.5 DNA Extraction and PCR Amplification of the Targeted Gene 3.6 Shrimp Alkaline Phosphatase Treatment3.7 Labeling; 3.8 Hybridization and Slide Washing; 3.9 Data Analysis; 3.10 Costs; 3.11 Microarray for Detection of Pathogenic Bacteria; References; 4 Non-Cognate Approaches for Pathogen Detection on Microarrays; 4.1 Introduction; 4.2 Non-Cognate Hybridization System; 4.2.1 Concept; 4.2.2 Definition of the Optimal Probe Length; 4.2.3 Virtual Assessment of Array Performances (in Silico Experiments); 4.2.4 Array Manufacturing and Hybridization (Wet-Lab Experiments); 4.2.5 Analysis; 4.3 Perspectives; References; 5 Patterning Techniques for Array Platforms 5.1 Introduction |
| Record Nr. | UNINA-9910143129503321 |
| Weinheim, : Wiley-VCH, 2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Detection of highly dangerous pathogens [[electronic resource] ] : microarray methods for BSL 3 and BSL 4 agents / / edited by Tanja Kostic, Patrick Butaye, and Jacques Schrenzel
| Detection of highly dangerous pathogens [[electronic resource] ] : microarray methods for BSL 3 and BSL 4 agents / / edited by Tanja Kostic, Patrick Butaye, and Jacques Schrenzel |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, 2009 |
| Descrizione fisica | 1 online resource (193 p.) |
| Disciplina | 579.165 |
| Altri autori (Persone) |
KosticTanja
ButayePatrick SchrenzelJacques |
| Soggetto topico |
Pathogenic microorganisms - Detection
DNA microarrays |
| ISBN |
1-282-29205-6
9786612292057 3-527-62668-9 3-527-62669-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Detection of Highly Dangerous Pathogens: Microarray Methods for the Detection of BSL 3 and BSL 4 Agents; Contents; This Publication is Supported by COST; Preface; List of Contributors; 1 Introduction to Microarray-Based Detection Methods; 1.1 Introduction to Microarray Technology; 1.2 Technical Aspects of Microarray Technology; 1.2.1 Probes; 1.2.1.1 Genome Fragments; 1.2.1.2 PCR Products; 1.2.1.3 Oligonucleotide Probes; 1.2.2 Substrates for Printing; 1.2.2.1 Slides with Poly-L-lysine Coating; 1.2.2.2 Slides with Amino Silane Coating; 1.2.2.3 Slides with Aldehyde Coating
1.2.2.4 Slides with Epoxy Coating1.2.2.5 Proprietary Surface Chemistries; 1.2.2.6 Probe Spacers; 1.2.3 Targets for Microarray Analysis; 1.2.3.1 Target Amplifications and Sensitivity Issues; 1.2.3.2 Labeling of the Targets; 1.2.3.3 Hybridization and Wash Conditions; 1.2.4 Classical Commercially Available Microarray Formats; 1.2.4.1 Spotting Approaches; 1.2.4.2 In Situ Synthesis; 1.2.5 Alternative Methods for Improving Microarray-Based Detection Sensitivity; 1.2.5.1 Resonance-Light Scattering (RLS); 1.2.5.2 Planar-Waveguide Technology (PWT); 1.2.5.3 Liquid Arrays 1.2.5.4 Three-Dimensional Microarray Formats1.2.6 Marker Genes Used on MDMs; 1.3 Analysis and Quality Control Aspects; 1.4 Applications of Microarray Technology in Microbial Diagnostics; 1.4.1 Gene Expression Studies; 1.4.2 Comparative Genomic Hybridization (CGH); 1.4.3 Generic or Universal Microarrays; 1.4.4 Microarrays for Sequence Analysis; 1.4.5 Microbial Diagnostic Microarrays (MDMs); 1.5 Further Developments and New Perspectives Regarding Array Sensitivity and Specificity; 1.6 Conclusions; References; Part I: Methods; 2 Long Oligonucleotide Microarray-Based Microbial Detection 2.1 Introduction2.2 Method; 2.2.1 DNA Extraction; 2.2.2 Ф29 Amplification; 2.2.3 Klenow Amplification/Labeling; 2.2.4 Probe and Slide Preparation; 2.2.5 Slide Processing Protocol (for Amino Surfaces); 2.2.6 Hybridization and Slide Washing; 2.2.7 Comments; 2.3 Our Test System and Results; 2.4 Conclusions; References; 3 Sequence-Specific End-Labeling of Oligonucleotides; 3.1 Introduction; 3.2 Probe Design; 3.3 Slide Preparation (Spotting); 3.4 Slide Processing Protocol ( for Aldehyde Surfaces); 3.5 DNA Extraction and PCR Amplification of the Targeted Gene 3.6 Shrimp Alkaline Phosphatase Treatment3.7 Labeling; 3.8 Hybridization and Slide Washing; 3.9 Data Analysis; 3.10 Costs; 3.11 Microarray for Detection of Pathogenic Bacteria; References; 4 Non-Cognate Approaches for Pathogen Detection on Microarrays; 4.1 Introduction; 4.2 Non-Cognate Hybridization System; 4.2.1 Concept; 4.2.2 Definition of the Optimal Probe Length; 4.2.3 Virtual Assessment of Array Performances (in Silico Experiments); 4.2.4 Array Manufacturing and Hybridization (Wet-Lab Experiments); 4.2.5 Analysis; 4.3 Perspectives; References; 5 Patterning Techniques for Array Platforms 5.1 Introduction |
| Record Nr. | UNINA-9910831037603321 |
| Weinheim, : Wiley-VCH, 2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||