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Handbook of fluorescence spectroscopy and imaging : from single molecules to ensembles / / Markus Sauer, Johan Hofkens, and Jorg Enderlein
| Handbook of fluorescence spectroscopy and imaging : from single molecules to ensembles / / Markus Sauer, Johan Hofkens, and Jorg Enderlein |
| Autore | Sauer Markus |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, 2011 |
| Descrizione fisica | 1 online resource (293 p.) |
| Disciplina | 543.56 |
| Altri autori (Persone) |
HofkensJohan
EnderleinJ (Jorg) |
| Soggetto topico |
Fluorescence spectroscopy
Fluorescence microscopy |
| ISBN |
3-527-63352-9
1-283-14063-2 9786613140630 3-527-63351-0 3-527-63350-2 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Handbook of Fluorescence Spectroscopy and Imaging: From Single Molecules to Ensembles; Contents; Preface; 1 Basic Principles of Fluorescence Spectroscopy; 1.1 Absorption and Emission of Light; 1.2 Spectroscopic Transition Strengths; 1.3 Lambert-Beer Law and Absorption Spectroscopy; 1.4 Fluorophore Dimerization and Isosbestic Points; 1.5 Franck-Condon Principle; 1.6 Temperature Effects on Absorption and Emission Spectra; 1.7 Fluorescence and Competing Processes; 1.8 Stokes Shift, Solvent Relaxation, and Solvatochroism; 1.9 Fluorescence Quantum Yield and Lifetime; 1.10 Fluorescence Anisotropy
References2 Fluorophores and Fluorescent Labels; 2.1 Natural Fluorophores; 2.2 Organic Fluorophores; 2.3 Different Fluorophore Classes; 2.4 Multichromophoric Labels; 2.5 Nanocrystals; References; 3 Fluorophore Labeling for Single-Molecule Fluorescence Spectroscopy (SMFS); 3.1 In Vitro Fluorescence Labeling; 3.2 Fluorescence Labeling in Living Cells; References; 4 Fluorophore Selection for Single-Molecule Fluorescence Spectroscopy (SMFS) and Photobleaching Pathways; References; 5 Fluorescence Correlation Spectroscopy; 5.1 Introduction; 5.2 Optical Set-Up; 5.3 Data Acquisition and Evaluation 5.4 Milliseconds to Seconds: Diffusion and Concentration5.4.1 Single-Focus FCS; 5.4.2 Dual-Focus FCS; 5.5 Nanoseconds to Microseconds: Photophysics, Conformational Fluctuations, Binding Dynamics; 5.6 Picoseconds to Nanoseconds: Rotational Diffusion and Fluorescence Antibunching; 5.6.1 Antibunching; 5.6.2 Rotational Diffusion; 5.7 Fluorescence Lifetime Correlation Spectroscopy; 5.8 Conclusion; References; 6 Excited State Energy Transfer; 6.1 Introduction; 6.2 Theory of (F€orster) Energy Transfer; 6.2.1 Mechanism and Mathematical Formalism of FRET 6.2.2 Measuring FRET Efficiencies Through Excited-State Lifetimes6.2.3 Spin Rules for FRET; 6.2.4 Homo-FRET and FRET-Induced Depolarization; 6.3 Experimental Approach for Single-Pair FRET-Experiments; 6.3.1 Single-Laser Excitation; 6.3.2 Alternating-Laser Excitation (ALEX); 6.4 Examples and Applications of FRET; 6.4.1 FRET Processes in Bulk Experiments; 6.4.1.1 FRET-Based Molecular Biosensors; 6.4.1.2 Energy Hopping and Trapping in Chromophore-Substituted Polyphenylene Dendrimers; 6.4.2 Single-Molecule Observation of FRET; 6.4.2.1 Light-Harvesting Systems: Phycobilisomes and Allophycocyanins 6.4.2.2 Hairpin Ribozyme Dynamics and Activity6.4.2.3 Protein (Un)folding and Dynamics; References; 7 Photoinduced Electron Transfer (PET) Reactions; 7.1 Fluorescence Quenching by PET; 7.2 Single-Molecule Fluorescence Spectroscopy to Study PET; 7.3 Single-Molecule Sensitive Fluorescence Sensors Based on PET; 7.4 PET Reporter System; 7.5 Monitoring Conformational Dynamics and Protein Folding by PET; 7.6 Biological and Diagnostic Applications; References; 8 Super-Resolution Fluorescence Imaging; 8.1 Diffraction Barrier of Optical Microscopy 8.2 Multi-Photon and Structured Illumination Microscopy |
| Record Nr. | UNINA-9910133642003321 |
Sauer Markus
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| Weinheim, : Wiley-VCH, 2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Nanomedicines for Effective Cancer Therapy / / edited by Hitoshi Kasai, Hiroshi Uji-i, Johan Hofkens
| Nanomedicines for Effective Cancer Therapy / / edited by Hitoshi Kasai, Hiroshi Uji-i, Johan Hofkens |
| Autore | Kasai Hitoshi |
| Edizione | [1st ed. 2024.] |
| Pubbl/distr/stampa | Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2024 |
| Descrizione fisica | 1 online resource (304 pages) |
| Disciplina | 620.19 |
| Altri autori (Persone) |
Uji-iHiroshi
HofkensJohan |
| Collana | Nanomedicine and Nanotoxicology |
| Soggetto topico |
Biomaterials
Cells Nanomedicine Medicinal chemistry Cancer - Treatment Drug delivery systems Materials - Microscopy Biomaterials-Cells Nanomedicine and Nanotoxicology Medicinal Chemistry Cancer Therapy Drug Delivery Microscopy |
| ISBN | 981-9752-88-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Most recent designs of nano-prodrugs for Hydroxy camptothecin and Podophyllotoxin -- Pt(II)-diradical complex toward theranostics of cancer -- Design of novel self assembling based drugs -- Innovative gene/nucleic acid delivery system based on optimized intracellular trafficking steps -- Novel targeted therapeutic nano-sized, micro-sized particles as DDS for brain tumor -- Designs of non-toxic and multifunctional nanocarriers for cancer therapy -- Cancer microenvironment-targeted therapy by molecular blocks -- Functional polymer-based siRNA delivery carrier that recognizes site-specific biosignals -- Nanowire-based nanoscopy for intracellular PH sensing -- Bioanalytical nanoprobes and microfluidicis -- Improvement of Multi-photon Microscopy by Utilizing Novel Optical Technologies and Materials -- Intracellular imaging using quantum dots -- Intracellular investigation of prodrug nanoparticles -- Polymeric Nanoparticles for Highly Efficient Multifunctional Drug Delivery Systems -- From 2D to 3D Cancer Cell Models—The Enigmas of Drug Delivery Research -- Control of the Formation Process of Polypeptide Self-assemblies for Understanding Complex Biological Systems: From Nano-physiology to Artificial Cells. |
| Record Nr. | UNINA-9910897991303321 |
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Kasai Hitoshi
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| Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2024 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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