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Bioseparations science and engineering / / Roger G. Harrison [and three others]
| Bioseparations science and engineering / / Roger G. Harrison [and three others] |
| Autore | Harrison Roger G. <1944-> |
| Edizione | [2nd edition.] |
| Pubbl/distr/stampa | New York : , : Oxford University Press, , 2015 |
| Descrizione fisica | 1 online resource (577 p.) |
| Disciplina | 660/.2842 |
| Collana | Topics in Chemical Engineering : A Series of Textbooks and Monographs |
| Soggetto topico |
Biomolecules - Separation
Biochemical engineering Separation (Technology) Extraction (Chemistry) |
| ISBN |
0-19-756276-0
0-19-021374-4 1-68015-865-1 9780195391817 0-19-021373-6 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Bioseparations Science and Engineering; Copyright; Dedication; Contents; Preface; 1 Introduction to Bioproducts and Bioseparations; 1.1 Instructional Objectives; 1.2 Broad Classification of Bioproducts; 1.3 Small Biomolecules; 1.3.1 Primary Metabolites; 1.3.2 Secondary Metabolites; 1.3.3 Summary of Small Biomolecules; 1.4 Macromolecules: Proteins; 1.4.1 Primary Structure; 1.4.2 Secondary Structure; 1.4.3 Tertiary Structure; Example 1.1 Effect of a Reducing Agent on Protein Structure and Mobility; 1.4.4 Quaternary Structure; 1.4.5 Prosthetic Groups and Hybrid Molecules
1.4.6 Functions and Commercial Uses of Proteins1.4.7 Stability of Proteins; 1.4.8 Recombinant Protein Expression; 1.5 Macromolecules: Nucleic Acids and Oligonucleotides; 1.6 Macromolecules: Polysaccharides; 1.7 Particulate Products; 1.8 Introduction to Bioseparations: Engineering Analysis; 1.8.1 Stages of Downstream Processing; Example 1.2 Initial Selection of Purification Steps; 1.8.2 Basic Principles of Engineering Analysis; 1.8.3 Process and Product Quality; 1.8.4 Criteria for Process Development; 1.9 The Route to Market; 1.9.1 The Chemical and Applications Range of the Bioproduct 1.9.2 Documentation of Pharmaceutical Bioproducts1.9.3 GLP and cGMP; 1.9.4 Formulation; 1.10 Summary; Nomenclature; Problems; References; 2 Analytical Methods and Bench Scale Preparative Bioseparations; 2.1 Instructional Objectives; 2.2 Specifications; 2.3 Assay Attributes; 2.3.1 Precision; 2.3.2 Accuracy; 2.3.3 Specificity; 2.3.4 Linearity, Limit of Detection, and Limit of Quantitation; 2.3.5 Range; 2.3.6 Robustness; 2.4 Analysis of Biological Activity; 2.4.1 Animal Model Assays; 2.4.2 Cell-Line-Derived Bioassays; 2.4.3 In vitro Biochemical Assays Example 2.1 Coupled Enzyme Assay for Alcohol Oxidase2.5 Analysis of Purity; 2.5.1 Electrophoretic Analysis; Example 2.2 Estimation of the Maximum Temperature in an Electrophoresis Gel; 2.5.2 High-Performance Liquid Chromatography (HPLC); 2.5.3 Mass Spectrometry; 2.5.4 Coupling of HPLC with Mass Spectrometry; 2.5.5 Ultraviolet Absorbance; Example 2.3 Determination of Molar Absorptivity; 2.5.6 CHNO/Amino Acid Analysis (AAA); Example 2.4 Calculations Based on CHNO Analysis; 2.5.7 Protein Assays; 2.5.8 Enzyme-Linked Immunosorbent Assay; 2.5.9 Gas Chromatography; 2.5.10 DNA Hybridization 2.5.11 ICP/MS (AES)2.5.12 Dry Weight; 2.6 Microbiology Assays; 2.6.1 Sterility; 2.6.2 Bioburden; 2.6.3 Endotoxin; 2.6.4 Virus, Mycoplasma, and Phage; 2.7 Bench Scale Preparative Separations; 2.7.1 Preparative Electrophoresis; 2.7.2 Magnetic Bioseparations; 2.8 Summary; Nomenclature; Problems; References; 3 Cell Lysis and Flocculation; 3.1 Instructional Objectives; 3.2 Some Elements of Cell Structure; 3.2.1 Prokaryotic Cells; 3.2.2 Eukaryotic Cells; 3.3 Cell Lysis; 3.3.1 Osmotic and Chemical Cell Lysis; 3.3.2 Mechanical Methods of Lysis; 3.4 Flocculation; 3.4.1 The Electric Double Layer Example 3.1 Dependence of the Debye Radius on the Type of Electrolyte |
| Record Nr. | UNINA-9910787493903321 |
Harrison Roger G. <1944->
|
||
| New York : , : Oxford University Press, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Bioseparations science and engineering / / Roger G. Harrison [and three others]
| Bioseparations science and engineering / / Roger G. Harrison [and three others] |
| Autore | Harrison Roger G. <1944-> |
| Edizione | [2nd edition.] |
| Pubbl/distr/stampa | New York : , : Oxford University Press, , 2015 |
| Descrizione fisica | 1 online resource (577 p.) |
| Disciplina | 660/.2842 |
| Collana | Topics in Chemical Engineering : A Series of Textbooks and Monographs |
| Soggetto topico |
Biomolecules - Separation
Biochemical engineering Separation (Technology) Extraction (Chemistry) |
| ISBN |
0-19-756276-0
0-19-021374-4 1-68015-865-1 9780195391817 0-19-021373-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Bioseparations Science and Engineering; Copyright; Dedication; Contents; Preface; 1 Introduction to Bioproducts and Bioseparations; 1.1 Instructional Objectives; 1.2 Broad Classification of Bioproducts; 1.3 Small Biomolecules; 1.3.1 Primary Metabolites; 1.3.2 Secondary Metabolites; 1.3.3 Summary of Small Biomolecules; 1.4 Macromolecules: Proteins; 1.4.1 Primary Structure; 1.4.2 Secondary Structure; 1.4.3 Tertiary Structure; Example 1.1 Effect of a Reducing Agent on Protein Structure and Mobility; 1.4.4 Quaternary Structure; 1.4.5 Prosthetic Groups and Hybrid Molecules
1.4.6 Functions and Commercial Uses of Proteins1.4.7 Stability of Proteins; 1.4.8 Recombinant Protein Expression; 1.5 Macromolecules: Nucleic Acids and Oligonucleotides; 1.6 Macromolecules: Polysaccharides; 1.7 Particulate Products; 1.8 Introduction to Bioseparations: Engineering Analysis; 1.8.1 Stages of Downstream Processing; Example 1.2 Initial Selection of Purification Steps; 1.8.2 Basic Principles of Engineering Analysis; 1.8.3 Process and Product Quality; 1.8.4 Criteria for Process Development; 1.9 The Route to Market; 1.9.1 The Chemical and Applications Range of the Bioproduct 1.9.2 Documentation of Pharmaceutical Bioproducts1.9.3 GLP and cGMP; 1.9.4 Formulation; 1.10 Summary; Nomenclature; Problems; References; 2 Analytical Methods and Bench Scale Preparative Bioseparations; 2.1 Instructional Objectives; 2.2 Specifications; 2.3 Assay Attributes; 2.3.1 Precision; 2.3.2 Accuracy; 2.3.3 Specificity; 2.3.4 Linearity, Limit of Detection, and Limit of Quantitation; 2.3.5 Range; 2.3.6 Robustness; 2.4 Analysis of Biological Activity; 2.4.1 Animal Model Assays; 2.4.2 Cell-Line-Derived Bioassays; 2.4.3 In vitro Biochemical Assays Example 2.1 Coupled Enzyme Assay for Alcohol Oxidase2.5 Analysis of Purity; 2.5.1 Electrophoretic Analysis; Example 2.2 Estimation of the Maximum Temperature in an Electrophoresis Gel; 2.5.2 High-Performance Liquid Chromatography (HPLC); 2.5.3 Mass Spectrometry; 2.5.4 Coupling of HPLC with Mass Spectrometry; 2.5.5 Ultraviolet Absorbance; Example 2.3 Determination of Molar Absorptivity; 2.5.6 CHNO/Amino Acid Analysis (AAA); Example 2.4 Calculations Based on CHNO Analysis; 2.5.7 Protein Assays; 2.5.8 Enzyme-Linked Immunosorbent Assay; 2.5.9 Gas Chromatography; 2.5.10 DNA Hybridization 2.5.11 ICP/MS (AES)2.5.12 Dry Weight; 2.6 Microbiology Assays; 2.6.1 Sterility; 2.6.2 Bioburden; 2.6.3 Endotoxin; 2.6.4 Virus, Mycoplasma, and Phage; 2.7 Bench Scale Preparative Separations; 2.7.1 Preparative Electrophoresis; 2.7.2 Magnetic Bioseparations; 2.8 Summary; Nomenclature; Problems; References; 3 Cell Lysis and Flocculation; 3.1 Instructional Objectives; 3.2 Some Elements of Cell Structure; 3.2.1 Prokaryotic Cells; 3.2.2 Eukaryotic Cells; 3.3 Cell Lysis; 3.3.1 Osmotic and Chemical Cell Lysis; 3.3.2 Mechanical Methods of Lysis; 3.4 Flocculation; 3.4.1 The Electric Double Layer Example 3.1 Dependence of the Debye Radius on the Type of Electrolyte |
| Record Nr. | UNINA-9910808432703321 |
|
Harrison Roger G. <1944->
|
||
| New York : , : Oxford University Press, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||