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DNA chromatography
| DNA chromatography |
| Autore | Gjerde Douglas T |
| Pubbl/distr/stampa | [Place of publication not identified], : Wiley VCH, 2002 |
| Descrizione fisica | 1 online resource (238 pages) |
| Disciplina | 572.8/6 |
| Soggetto topico |
DNA - Analysis
Chromatographic analysis DNA Chromatography, High Pressure Liquid Genetic Techniques Investigative Techniques Nucleic Acids Chromatography, Liquid Nucleic Acids, Nucleotides, and Nucleosides Analytical, Diagnostic and Therapeutic Techniques and Equipment Chromatography Chemistry Techniques, Analytical Chemicals and Drugs Animal Biochemistry Human Anatomy & Physiology Health & Biological Sciences |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-280-55844-X
9786610558445 3-527-60074-4 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910146247903321 |
Gjerde Douglas T
|
||
| [Place of publication not identified], : Wiley VCH, 2002 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
DNA chromatography
| DNA chromatography |
| Autore | Gjerde Douglas T |
| Pubbl/distr/stampa | [Place of publication not identified], : Wiley VCH, 2002 |
| Descrizione fisica | 1 online resource (238 pages) |
| Disciplina | 572.8/6 |
| Soggetto topico |
DNA - Analysis
Chromatographic analysis DNA Chromatography, High Pressure Liquid Genetic Techniques Investigative Techniques Nucleic Acids Chromatography, Liquid Nucleic Acids, Nucleotides, and Nucleosides Analytical, Diagnostic and Therapeutic Techniques and Equipment Chromatography Chemistry Techniques, Analytical Chemicals and Drugs Animal Biochemistry Human Anatomy & Physiology Health & Biological Sciences |
| ISBN |
1-280-55844-X
9786610558445 3-527-60074-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910830366003321 |
|
Gjerde Douglas T
|
||
| [Place of publication not identified], : Wiley VCH, 2002 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
DNA chromatography
| DNA chromatography |
| Autore | Gjerde Douglas T |
| Pubbl/distr/stampa | [Place of publication not identified], : Wiley VCH, 2002 |
| Descrizione fisica | 1 online resource (238 pages) |
| Disciplina | 572.8/6 |
| Soggetto topico |
DNA - Analysis
Chromatographic analysis DNA Chromatography, High Pressure Liquid Genetic Techniques Investigative Techniques Nucleic Acids Chromatography, Liquid Nucleic Acids, Nucleotides, and Nucleosides Analytical, Diagnostic and Therapeutic Techniques and Equipment Chromatography Chemistry Techniques, Analytical Chemicals and Drugs Animal Biochemistry Human Anatomy & Physiology Health & Biological Sciences |
| ISBN |
1-280-55844-X
9786610558445 3-527-60074-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910877038103321 |
|
Gjerde Douglas T
|
||
| [Place of publication not identified], : Wiley VCH, 2002 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
RNA purification and analysis [[electronic resource] ] : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby
| RNA purification and analysis [[electronic resource] ] : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby |
| Autore | Gjerde Douglas T |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH |
| Descrizione fisica | 1 online resource (209 p.) |
| Disciplina |
572.88
572.8836 574.87/3283 |
| Altri autori (Persone) |
HornbyDavid, Dr.
HoangLee |
| Soggetto topico |
RNA - Analysis
RNA - Purification |
| ISBN |
1-282-68979-7
9786612689796 3-527-62719-7 3-527-62720-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
RNA Purification and Analysis: Sample Preparation, Extraction, Chromatography; Contents; Preface; Acknowledgments; 1: RNA Extraction, Separation, and Analysis; 1.1 The Need to Be Able to Extract, Manipulate, and Analyze RNA; 1.2 Using Chemical Tools to Solve the Problem of Analysis of Biological Processes; 1.3 The Principle of Chromatography and Solid-Phase Extraction; 1.3.1 Principle of Chromatography; 1.3.2 Mobile Phase Gradient Controls Elution; 1.3.3 Different Types of Column and Eluent Chemistries; 1.3.4 The Principle of Solid-Phase Extraction; 1.4 RNA Chromatography
1.5 Enzymatic Treatment of RNA and Analysis1.5.1 Polyacrylamide Gel Electrophoresis; 1.5.2 RNA Structure Probing with Ribonuclease Enzymes; 1.6 Content and Organization of This Book; References; 2: Biological and Chemical RNA; 2.1 Why Classify RNA with Biology and Chemistry?; 2.1.1 Chemical Classification of RNA; 2.1.2 Biological Classification of RNA; 2.2 Prokaryotic Cellular RNA; 2.3 Prokaryote Sample Type; 2.3.1 Escherichia coli; 2.3.2 Other Bacteria; 2.4 Eukaryotic Cellular RNA; 2.5 Eukaryote Sample Type; 2.5.1 Yeast; 2.5.2 Other Fungi; 2.5.3 Simple Multicellular Organism 2.5.4 Soft Animal2.5.5 Hard Animal; 2.5.6 Plant; 2.5.7 Cell Culture; 2.6 Other Samples; 2.6.1 Virus; 2.6.2 Soil and Rock; 2.7 Synthetic RNA; 2.7.1 Aptamers; 2.7.2 SELEX; 2.7.3 Short Hairpin RNAs; References; 3: RNA Separation: Substrates, Functional Groups, Mechanisms, and Control; 3.1 Solid-Phase Interaction; 3.1.1 Adsorption of Sample Compounds and Sample Matrix Compounds; 3.1.2 Roles of Solid-Phase Substrate and Functional Group; 3.1.3 Correlation of Interaction Type, Functional Group, and Substrate; 3.1.4 RNA Structure and Solid Surface Interaction 3.2 The Solid-Phase Substrate and Attachment of Functional Groups3.2.1 Polymeric Resin Substrates; 3.2.2 Porous and Nonporous Polymeric Resins; 3.2.3 Monolith Polymeric Columns; 3.2.4 Functionalization of the Polymer; 3.2.5 Silica-Glass-Based Substrates; 3.2.6 Functionalization of Silica; 3.2.7 Agarose and Cellulose Affinity Substrates; 3.2.8 Dextran and Polyacrylamide Gel Filtration Substrates; 3.3 Reverse-Phase Ion-Pairing Separation Mechanism; 3.4 Ion-Exchange Separation Mechanism; 3.5 Chaotropic Denaturing Interaction Mechanism; 3.6 Hybridization; 3.6.1 SELEX; 3.7 Gel Filtration References4: RNA Extraction and Analysis; 4.1 Transcription; 4.1.1 RNA Catalysis; 4.1.2 RNA-Protein Complex Interactions; 4.1.3 Pre-mRNA Splicing; 4.2 Translation; 4.2.1 Post-Transcriptional Control of Eukaryotic Gene Expression; 4.3 Gene Regulation; 4.3.1 RNA Interference Pathway; 4.3.2 Micro RNAs and Their Role in Gene Regulation; 4.4 Use of siRNA to Investigate Gene Function; References; 5: RNA Chromatography; 5.1 Development of RNA Chromatography; 5.2 RNA Chromatography Instrumentation; 5.2.1 The Column Oven; 5.2.2 Ultraviolet (UV) and Fluorescence Detection; 5.2.3 Fragment Collection 5.3 RNA Chromatography Conditions |
| Record Nr. | UNINA-9910139923703321 |
|
Gjerde Douglas T
|
||
| Weinheim, : Wiley-VCH | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
RNA purification and analysis [[electronic resource] ] : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby
| RNA purification and analysis [[electronic resource] ] : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby |
| Autore | Gjerde Douglas T |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH |
| Descrizione fisica | 1 online resource (209 p.) |
| Disciplina |
572.88
572.8836 574.87/3283 |
| Altri autori (Persone) |
HornbyDavid, Dr.
HoangLee |
| Soggetto topico |
RNA - Analysis
RNA - Purification |
| ISBN |
1-282-68979-7
9786612689796 3-527-62719-7 3-527-62720-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
RNA Purification and Analysis: Sample Preparation, Extraction, Chromatography; Contents; Preface; Acknowledgments; 1: RNA Extraction, Separation, and Analysis; 1.1 The Need to Be Able to Extract, Manipulate, and Analyze RNA; 1.2 Using Chemical Tools to Solve the Problem of Analysis of Biological Processes; 1.3 The Principle of Chromatography and Solid-Phase Extraction; 1.3.1 Principle of Chromatography; 1.3.2 Mobile Phase Gradient Controls Elution; 1.3.3 Different Types of Column and Eluent Chemistries; 1.3.4 The Principle of Solid-Phase Extraction; 1.4 RNA Chromatography
1.5 Enzymatic Treatment of RNA and Analysis1.5.1 Polyacrylamide Gel Electrophoresis; 1.5.2 RNA Structure Probing with Ribonuclease Enzymes; 1.6 Content and Organization of This Book; References; 2: Biological and Chemical RNA; 2.1 Why Classify RNA with Biology and Chemistry?; 2.1.1 Chemical Classification of RNA; 2.1.2 Biological Classification of RNA; 2.2 Prokaryotic Cellular RNA; 2.3 Prokaryote Sample Type; 2.3.1 Escherichia coli; 2.3.2 Other Bacteria; 2.4 Eukaryotic Cellular RNA; 2.5 Eukaryote Sample Type; 2.5.1 Yeast; 2.5.2 Other Fungi; 2.5.3 Simple Multicellular Organism 2.5.4 Soft Animal2.5.5 Hard Animal; 2.5.6 Plant; 2.5.7 Cell Culture; 2.6 Other Samples; 2.6.1 Virus; 2.6.2 Soil and Rock; 2.7 Synthetic RNA; 2.7.1 Aptamers; 2.7.2 SELEX; 2.7.3 Short Hairpin RNAs; References; 3: RNA Separation: Substrates, Functional Groups, Mechanisms, and Control; 3.1 Solid-Phase Interaction; 3.1.1 Adsorption of Sample Compounds and Sample Matrix Compounds; 3.1.2 Roles of Solid-Phase Substrate and Functional Group; 3.1.3 Correlation of Interaction Type, Functional Group, and Substrate; 3.1.4 RNA Structure and Solid Surface Interaction 3.2 The Solid-Phase Substrate and Attachment of Functional Groups3.2.1 Polymeric Resin Substrates; 3.2.2 Porous and Nonporous Polymeric Resins; 3.2.3 Monolith Polymeric Columns; 3.2.4 Functionalization of the Polymer; 3.2.5 Silica-Glass-Based Substrates; 3.2.6 Functionalization of Silica; 3.2.7 Agarose and Cellulose Affinity Substrates; 3.2.8 Dextran and Polyacrylamide Gel Filtration Substrates; 3.3 Reverse-Phase Ion-Pairing Separation Mechanism; 3.4 Ion-Exchange Separation Mechanism; 3.5 Chaotropic Denaturing Interaction Mechanism; 3.6 Hybridization; 3.6.1 SELEX; 3.7 Gel Filtration References4: RNA Extraction and Analysis; 4.1 Transcription; 4.1.1 RNA Catalysis; 4.1.2 RNA-Protein Complex Interactions; 4.1.3 Pre-mRNA Splicing; 4.2 Translation; 4.2.1 Post-Transcriptional Control of Eukaryotic Gene Expression; 4.3 Gene Regulation; 4.3.1 RNA Interference Pathway; 4.3.2 Micro RNAs and Their Role in Gene Regulation; 4.4 Use of siRNA to Investigate Gene Function; References; 5: RNA Chromatography; 5.1 Development of RNA Chromatography; 5.2 RNA Chromatography Instrumentation; 5.2.1 The Column Oven; 5.2.2 Ultraviolet (UV) and Fluorescence Detection; 5.2.3 Fragment Collection 5.3 RNA Chromatography Conditions |
| Record Nr. | UNINA-9910829855103321 |
|
Gjerde Douglas T
|
||
| Weinheim, : Wiley-VCH | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
RNA purification and analysis : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby
| RNA purification and analysis : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby |
| Autore | Gjerde Douglas T |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH |
| Descrizione fisica | 1 online resource (209 p.) |
| Disciplina |
572.88
572.8836 574.87/3283 |
| Altri autori (Persone) |
HornbyDavid, Dr.
HoangLee |
| Soggetto topico |
RNA - Analysis
RNA - Purification |
| ISBN |
1-282-68979-7
9786612689796 3-527-62719-7 3-527-62720-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
RNA Purification and Analysis: Sample Preparation, Extraction, Chromatography; Contents; Preface; Acknowledgments; 1: RNA Extraction, Separation, and Analysis; 1.1 The Need to Be Able to Extract, Manipulate, and Analyze RNA; 1.2 Using Chemical Tools to Solve the Problem of Analysis of Biological Processes; 1.3 The Principle of Chromatography and Solid-Phase Extraction; 1.3.1 Principle of Chromatography; 1.3.2 Mobile Phase Gradient Controls Elution; 1.3.3 Different Types of Column and Eluent Chemistries; 1.3.4 The Principle of Solid-Phase Extraction; 1.4 RNA Chromatography
1.5 Enzymatic Treatment of RNA and Analysis1.5.1 Polyacrylamide Gel Electrophoresis; 1.5.2 RNA Structure Probing with Ribonuclease Enzymes; 1.6 Content and Organization of This Book; References; 2: Biological and Chemical RNA; 2.1 Why Classify RNA with Biology and Chemistry?; 2.1.1 Chemical Classification of RNA; 2.1.2 Biological Classification of RNA; 2.2 Prokaryotic Cellular RNA; 2.3 Prokaryote Sample Type; 2.3.1 Escherichia coli; 2.3.2 Other Bacteria; 2.4 Eukaryotic Cellular RNA; 2.5 Eukaryote Sample Type; 2.5.1 Yeast; 2.5.2 Other Fungi; 2.5.3 Simple Multicellular Organism 2.5.4 Soft Animal2.5.5 Hard Animal; 2.5.6 Plant; 2.5.7 Cell Culture; 2.6 Other Samples; 2.6.1 Virus; 2.6.2 Soil and Rock; 2.7 Synthetic RNA; 2.7.1 Aptamers; 2.7.2 SELEX; 2.7.3 Short Hairpin RNAs; References; 3: RNA Separation: Substrates, Functional Groups, Mechanisms, and Control; 3.1 Solid-Phase Interaction; 3.1.1 Adsorption of Sample Compounds and Sample Matrix Compounds; 3.1.2 Roles of Solid-Phase Substrate and Functional Group; 3.1.3 Correlation of Interaction Type, Functional Group, and Substrate; 3.1.4 RNA Structure and Solid Surface Interaction 3.2 The Solid-Phase Substrate and Attachment of Functional Groups3.2.1 Polymeric Resin Substrates; 3.2.2 Porous and Nonporous Polymeric Resins; 3.2.3 Monolith Polymeric Columns; 3.2.4 Functionalization of the Polymer; 3.2.5 Silica-Glass-Based Substrates; 3.2.6 Functionalization of Silica; 3.2.7 Agarose and Cellulose Affinity Substrates; 3.2.8 Dextran and Polyacrylamide Gel Filtration Substrates; 3.3 Reverse-Phase Ion-Pairing Separation Mechanism; 3.4 Ion-Exchange Separation Mechanism; 3.5 Chaotropic Denaturing Interaction Mechanism; 3.6 Hybridization; 3.6.1 SELEX; 3.7 Gel Filtration References4: RNA Extraction and Analysis; 4.1 Transcription; 4.1.1 RNA Catalysis; 4.1.2 RNA-Protein Complex Interactions; 4.1.3 Pre-mRNA Splicing; 4.2 Translation; 4.2.1 Post-Transcriptional Control of Eukaryotic Gene Expression; 4.3 Gene Regulation; 4.3.1 RNA Interference Pathway; 4.3.2 Micro RNAs and Their Role in Gene Regulation; 4.4 Use of siRNA to Investigate Gene Function; References; 5: RNA Chromatography; 5.1 Development of RNA Chromatography; 5.2 RNA Chromatography Instrumentation; 5.2.1 The Column Oven; 5.2.2 Ultraviolet (UV) and Fluorescence Detection; 5.2.3 Fragment Collection 5.3 RNA Chromatography Conditions |
| Record Nr. | UNINA-9910876677603321 |
|
Gjerde Douglas T
|
||
| Weinheim, : Wiley-VCH | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||