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Advanced manufacturing technology for medical applications [[electronic resource] ] : reverse engineering, software conversion, and rapid prototyping / / edited by Ian Gibson
| Advanced manufacturing technology for medical applications [[electronic resource] ] : reverse engineering, software conversion, and rapid prototyping / / edited by Ian Gibson |
| Pubbl/distr/stampa | Chichester, West Sussex, England ; ; Hoboken, NJ, USA, : J. Wiley, c2005 |
| Descrizione fisica | 1 online resource (256 p.) |
| Disciplina |
610.28
610/.28 |
| Altri autori (Persone) | GibsonIan <1938-> |
| Collana | Engineering Research Series (REP) |
| Soggetto topico |
Medical technology
Medical innovations Manufacturing processes |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-280-35585-9
9786610355853 0-470-03398-3 1-60119-372-6 0-470-03284-7 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Advanced Manufacturing Technology for Medical Applications; Contents; Contributors; 1 Rapid Prototyping for Medical Applications; 1.1 Overview; 1.2 Workshop on Medical Applications for Reverse Engineering and Rapid Prototyping; 1.3 Purpose of This Chapter (Overview); 1.4 Background on Rapid Prototyping; 1.5 Stereolithography and Other Resin-type Systems; 1.6 Fused Deposition Modelling and Selective Laser Sintering; 1.7 Droplet/Binder Systems; 1.8 Related Technology: Microsystems and Direct Metal Systems; 1.9 File Preparation; 1.10 Relationship with Other Technologies
1.11 Disadvantages with RP for Medical Applications1.12 Summary; Bibliography; 2 Role of Rapid Digital Manufacture in Planning and Implementation of Complex Medical Treatments; 2.1 Introduction; 2.2 Primer on Medical Imaging; 2.3 Surgical Planning; 2.3.1 Virtual planning; 2.3.2 Implementation of the plan; 2.4 RDM in Medicine; 2.4.1 RP-generated anatomical models; 2.4.2 Custom treatment devices with ADM; 2.5 The Future; 2.6 Conclusion; References; 3 Biomodelling; 3.1 Introduction; 3.2 Surgical Applications of Real Virtuality; 3.2.1 Cranio-maxillofacial biomodelling 3.2.1.1 Integration of biomodels with dental castings3.2.1.2 Use of biomodels to shape maxillofacial implants; 3.2.1.3 Use of biomodels to prefabricate templates and splints; 3.2.1.4 Use of biomodels in restorative prosthetics; 3.2.2 Use of real virtuality in customized cranio-maxillofacial prosthetics; 3.2.2.1 Computer mirroring techniques for the generation of prostheses; 3.2.2.2 Results of implantation; 3.2.2.3 Advantages of prefabricated customized cranioplastic implants; 3.2.3 Biomodel-guided stereotaxy; 3.2.3.1 Development of stereotaxy 3.2.3.2 Development of biomodel-guided stereotactic surgery3.2.3.3 Biomodel-guided stereotactic surgery with a template and markers; 3.2.3.4 Biomodel-guided stereotactic surgery using the D'Urso frame; 3.2.3.5 Utility of biomodel-guided stereotactic surgery; 3.2.4 Vascular biomodelling; 3.2.4.1 Biomodelling from CTA; 3.2.4.2 Biomodelling from MRA; 3.2.4.3 Clinical applications of vascular biomodels; 3.2.4.4 Vascular biomodelling: technical note; 3.2.5 Skull-base tumour surgery; 3.2.6 Spinal surgery; 3.2.6.1 Spinal biomodel stereotaxy; 3.2.6.2 Technical considerations in spinal biomodelling 3.2.7 Orthopaedic biomodelling3.3 Case Studies; References; 4 Three-dimensional Data Capture and Processing; 4.1 Introduction; 4.2 3D Medical Scan Process; 4.2.1 3D scanning; 4.2.1.1 Computed tomography imaging and its applications; 4.2.1.2 Magnetic resonance imaging and its applications; 4.2.1.3 Ultrasound imaging and its applications; 4.2.1.4 3D laser scanning; 4.2.2 3D reconstruction; 4.3 RE and RP in Medical Application; 4.3.1 Proposed method for RP model construction from scanned data; 4.3.2 Reconstruction software; 4.3.3 Accuracy issues; 4.4 Applications of Medical Imaging 4.5 Case Study |
| Record Nr. | UNINA-9910143727503321 |
| Chichester, West Sussex, England ; ; Hoboken, NJ, USA, : J. Wiley, c2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Advanced manufacturing technology for medical applications [[electronic resource] ] : reverse engineering, software conversion, and rapid prototyping / / edited by Ian Gibson
| Advanced manufacturing technology for medical applications [[electronic resource] ] : reverse engineering, software conversion, and rapid prototyping / / edited by Ian Gibson |
| Pubbl/distr/stampa | Chichester, West Sussex, England ; ; Hoboken, NJ, USA, : J. Wiley, c2005 |
| Descrizione fisica | 1 online resource (256 p.) |
| Disciplina |
610.28
610/.28 |
| Altri autori (Persone) | GibsonIan <1938-> |
| Collana | Engineering Research Series (REP) |
| Soggetto topico |
Medical technology
Medical innovations Manufacturing processes |
| ISBN |
1-280-35585-9
9786610355853 0-470-03398-3 1-60119-372-6 0-470-03284-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Advanced Manufacturing Technology for Medical Applications; Contents; Contributors; 1 Rapid Prototyping for Medical Applications; 1.1 Overview; 1.2 Workshop on Medical Applications for Reverse Engineering and Rapid Prototyping; 1.3 Purpose of This Chapter (Overview); 1.4 Background on Rapid Prototyping; 1.5 Stereolithography and Other Resin-type Systems; 1.6 Fused Deposition Modelling and Selective Laser Sintering; 1.7 Droplet/Binder Systems; 1.8 Related Technology: Microsystems and Direct Metal Systems; 1.9 File Preparation; 1.10 Relationship with Other Technologies
1.11 Disadvantages with RP for Medical Applications1.12 Summary; Bibliography; 2 Role of Rapid Digital Manufacture in Planning and Implementation of Complex Medical Treatments; 2.1 Introduction; 2.2 Primer on Medical Imaging; 2.3 Surgical Planning; 2.3.1 Virtual planning; 2.3.2 Implementation of the plan; 2.4 RDM in Medicine; 2.4.1 RP-generated anatomical models; 2.4.2 Custom treatment devices with ADM; 2.5 The Future; 2.6 Conclusion; References; 3 Biomodelling; 3.1 Introduction; 3.2 Surgical Applications of Real Virtuality; 3.2.1 Cranio-maxillofacial biomodelling 3.2.1.1 Integration of biomodels with dental castings3.2.1.2 Use of biomodels to shape maxillofacial implants; 3.2.1.3 Use of biomodels to prefabricate templates and splints; 3.2.1.4 Use of biomodels in restorative prosthetics; 3.2.2 Use of real virtuality in customized cranio-maxillofacial prosthetics; 3.2.2.1 Computer mirroring techniques for the generation of prostheses; 3.2.2.2 Results of implantation; 3.2.2.3 Advantages of prefabricated customized cranioplastic implants; 3.2.3 Biomodel-guided stereotaxy; 3.2.3.1 Development of stereotaxy 3.2.3.2 Development of biomodel-guided stereotactic surgery3.2.3.3 Biomodel-guided stereotactic surgery with a template and markers; 3.2.3.4 Biomodel-guided stereotactic surgery using the D'Urso frame; 3.2.3.5 Utility of biomodel-guided stereotactic surgery; 3.2.4 Vascular biomodelling; 3.2.4.1 Biomodelling from CTA; 3.2.4.2 Biomodelling from MRA; 3.2.4.3 Clinical applications of vascular biomodels; 3.2.4.4 Vascular biomodelling: technical note; 3.2.5 Skull-base tumour surgery; 3.2.6 Spinal surgery; 3.2.6.1 Spinal biomodel stereotaxy; 3.2.6.2 Technical considerations in spinal biomodelling 3.2.7 Orthopaedic biomodelling3.3 Case Studies; References; 4 Three-dimensional Data Capture and Processing; 4.1 Introduction; 4.2 3D Medical Scan Process; 4.2.1 3D scanning; 4.2.1.1 Computed tomography imaging and its applications; 4.2.1.2 Magnetic resonance imaging and its applications; 4.2.1.3 Ultrasound imaging and its applications; 4.2.1.4 3D laser scanning; 4.2.2 3D reconstruction; 4.3 RE and RP in Medical Application; 4.3.1 Proposed method for RP model construction from scanned data; 4.3.2 Reconstruction software; 4.3.3 Accuracy issues; 4.4 Applications of Medical Imaging 4.5 Case Study |
| Record Nr. | UNINA-9910830536303321 |
| Chichester, West Sussex, England ; ; Hoboken, NJ, USA, : J. Wiley, c2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Advanced manufacturing technology for medical applications [[electronic resource] ] : reverse engineering, software conversion, and rapid prototyping / / edited by Ian Gibson
| Advanced manufacturing technology for medical applications [[electronic resource] ] : reverse engineering, software conversion, and rapid prototyping / / edited by Ian Gibson |
| Pubbl/distr/stampa | Chichester, West Sussex, England ; ; Hoboken, NJ, USA, : J. Wiley, c2005 |
| Descrizione fisica | 1 online resource (256 p.) |
| Disciplina |
610.28
610/.28 |
| Altri autori (Persone) | GibsonIan <1938-> |
| Collana | Engineering Research Series (REP) |
| Soggetto topico |
Medical technology
Medical innovations Manufacturing processes |
| ISBN |
1-280-35585-9
9786610355853 0-470-03398-3 1-60119-372-6 0-470-03284-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Advanced Manufacturing Technology for Medical Applications; Contents; Contributors; 1 Rapid Prototyping for Medical Applications; 1.1 Overview; 1.2 Workshop on Medical Applications for Reverse Engineering and Rapid Prototyping; 1.3 Purpose of This Chapter (Overview); 1.4 Background on Rapid Prototyping; 1.5 Stereolithography and Other Resin-type Systems; 1.6 Fused Deposition Modelling and Selective Laser Sintering; 1.7 Droplet/Binder Systems; 1.8 Related Technology: Microsystems and Direct Metal Systems; 1.9 File Preparation; 1.10 Relationship with Other Technologies
1.11 Disadvantages with RP for Medical Applications1.12 Summary; Bibliography; 2 Role of Rapid Digital Manufacture in Planning and Implementation of Complex Medical Treatments; 2.1 Introduction; 2.2 Primer on Medical Imaging; 2.3 Surgical Planning; 2.3.1 Virtual planning; 2.3.2 Implementation of the plan; 2.4 RDM in Medicine; 2.4.1 RP-generated anatomical models; 2.4.2 Custom treatment devices with ADM; 2.5 The Future; 2.6 Conclusion; References; 3 Biomodelling; 3.1 Introduction; 3.2 Surgical Applications of Real Virtuality; 3.2.1 Cranio-maxillofacial biomodelling 3.2.1.1 Integration of biomodels with dental castings3.2.1.2 Use of biomodels to shape maxillofacial implants; 3.2.1.3 Use of biomodels to prefabricate templates and splints; 3.2.1.4 Use of biomodels in restorative prosthetics; 3.2.2 Use of real virtuality in customized cranio-maxillofacial prosthetics; 3.2.2.1 Computer mirroring techniques for the generation of prostheses; 3.2.2.2 Results of implantation; 3.2.2.3 Advantages of prefabricated customized cranioplastic implants; 3.2.3 Biomodel-guided stereotaxy; 3.2.3.1 Development of stereotaxy 3.2.3.2 Development of biomodel-guided stereotactic surgery3.2.3.3 Biomodel-guided stereotactic surgery with a template and markers; 3.2.3.4 Biomodel-guided stereotactic surgery using the D'Urso frame; 3.2.3.5 Utility of biomodel-guided stereotactic surgery; 3.2.4 Vascular biomodelling; 3.2.4.1 Biomodelling from CTA; 3.2.4.2 Biomodelling from MRA; 3.2.4.3 Clinical applications of vascular biomodels; 3.2.4.4 Vascular biomodelling: technical note; 3.2.5 Skull-base tumour surgery; 3.2.6 Spinal surgery; 3.2.6.1 Spinal biomodel stereotaxy; 3.2.6.2 Technical considerations in spinal biomodelling 3.2.7 Orthopaedic biomodelling3.3 Case Studies; References; 4 Three-dimensional Data Capture and Processing; 4.1 Introduction; 4.2 3D Medical Scan Process; 4.2.1 3D scanning; 4.2.1.1 Computed tomography imaging and its applications; 4.2.1.2 Magnetic resonance imaging and its applications; 4.2.1.3 Ultrasound imaging and its applications; 4.2.1.4 3D laser scanning; 4.2.2 3D reconstruction; 4.3 RE and RP in Medical Application; 4.3.1 Proposed method for RP model construction from scanned data; 4.3.2 Reconstruction software; 4.3.3 Accuracy issues; 4.4 Applications of Medical Imaging 4.5 Case Study |
| Record Nr. | UNINA-9910841050003321 |
| Chichester, West Sussex, England ; ; Hoboken, NJ, USA, : J. Wiley, c2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Antisense and ribozyme methodology / / Ian Gibson, editor
| Antisense and ribozyme methodology / / Ian Gibson, editor |
| Pubbl/distr/stampa | London, [England] : , : Chapman & Hall, , 1997 |
| Descrizione fisica | 1 online resource (96 p.) |
| Disciplina |
572.88
615.36 |
| Collana | Laboratory Companion Series |
| Soggetto topico |
Antisense nucleic acids
Catalytic RNA |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-84239-7
9786611842390 3-527-61253-X 3-527-61252-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Antisense and Ribozyme Methodology; Contents; CHAPTER 1. Antisense and Ribozyme Methodology; 1.1 The Potential; 1.2 Antisense Technology; 1.2.1 Problems; 1.2.2 Resistance to Nucleases; 1.2.3 Entry into Cells; 1.2.4 How Antisense Works; 1.2.5 Success; 1.3 Ribozymes; 1.3.1 What Are They?; 1.3.2 Problems; 1.3.3 Stable Ribozymes; 1.3.4 Designing Ribozymes; 1.4 Ribozymes or Antisense DNAs?; 1.5 The Choice Today!!; CHAPTER 2. Design and Synthesis of Antisense DNA Molecules; 2.1 Introduction; 2.2 Synthesis of Methylphosphonodiester-Phosphodiester Chimeric Oligodeoxynucleotides
2.2.1 Materials and Chemicals2.2.2 Solutions; 2.2.3 Maximizing Product Purity; 2.2.4 Deprotection of Chimeric Oligodeoxynucleotides; 2.2.5 Failed Sequences; 2.3 Primary Purification by Reversed-Phase, Solid-Phase Extraction on C18 SEP-PAK Cartridges; 2.3.1 Equipment; 2.3.2 Method; 2.3.3 Purification of the Oligodeoxynucleotide; 2.3.4 Further Purification; 2.4 Analysis and Purification by HPLC; 2.4.1 Analysis of Chimeric Oligodeoxynucleotides by HPLC; 2.4.2 Purification of Chimeric Oligodeoxynucleotides by HPLC; 2.4.3 Re-Use of Columns 2.5 Synthesis of Chimeric Oligodeoxynucleotides with Fluorescein Attached2.6 Summary; CHAPTER 3. The Design and Synthesis of Hammerhead Ribozymes; 3.1 Introduction; 3.2 The Design of Hammerhead Ribozymes; 3.3 Improving the Reactions; 3.3.1 Accessibility of the Target - Substrate Binding; 3.3.2 Finding the Target; 3.3.3 Theoretical Considerations; 3.3.4 Experimental Approaches; 3.3.5 Kinetic Studies; 3.4 Length of Arms; 3.4.1 Choosing Antisense Arms of Hammerhead Ribozymes; 3.4.2 Arms of Different Lengths; 3.5 Cleavage of the Target Motif; 3.6 Synthesis of Ribozymes 3.6.1 Chemical Synthesis of Short Hammerhead Ribozymes3.6.2 Enzymatic Transcription in Vitro; 3.7 Endogenous Expression of Ribozyme Genes; CHAPTER 4. Delivery of Ribozymes and Antisense DNA Molecules into Mammalian Cells; 4.1 Introduction; 4.2 Exogenous Application; 4.2.1 lntracytoplasmic Delivery of Antisense Oligodeoxynucleotides by Reversible Plasma Membrane Permeabilization with Streptolysin O; 4.3 Microinjection; 4.4 Other Methods Used in Nucleic Acid Transfection; 4.5 Electroporation; 4.5.1 Method; 4.5.2 Transfection: Optimization of Conditions 4.5.3 Mechanism of Uptake Following Electroporation4.5.4 Benefits and Drawbacks of Electrophoretic-Mediated Uptake; 4.6 Diethylaminoethyl-Dextran (DEAE- Dextran) and DNA Transfection; 4.6.1 Methods for Transfection of Adherent Cells; 4.6.2 Possible Alterations of the Above Protocol; 4.6.3 Transfection of Cells Growing in Suspension; 4.6.4 Transfection Optimization; 4.6.5 Distribution Mechanism of DEAE-Dextran Uptake and lntracellular; 4.6.6 Benefits and Drawbacks; 4.7 Calcium Phosphate Transfection; 4.7.1 Method; 4.7.2 Possible Alterations to Above Method; 4.7.3 Method Optimization 4.7.4 Calcium Phosphate-Mediated Uptake and lntracellular Distribution |
| Record Nr. | UNINA-9910144117903321 |
| London, [England] : , : Chapman & Hall, , 1997 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Antisense and ribozyme methodology / / Ian Gibson, editor
| Antisense and ribozyme methodology / / Ian Gibson, editor |
| Pubbl/distr/stampa | London, [England] : , : Chapman & Hall, , 1997 |
| Descrizione fisica | 1 online resource (96 p.) |
| Disciplina |
572.88
615.36 |
| Collana | Laboratory Companion Series |
| Soggetto topico |
Antisense nucleic acids
Catalytic RNA |
| ISBN |
1-281-84239-7
9786611842390 3-527-61253-X 3-527-61252-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Antisense and Ribozyme Methodology; Contents; CHAPTER 1. Antisense and Ribozyme Methodology; 1.1 The Potential; 1.2 Antisense Technology; 1.2.1 Problems; 1.2.2 Resistance to Nucleases; 1.2.3 Entry into Cells; 1.2.4 How Antisense Works; 1.2.5 Success; 1.3 Ribozymes; 1.3.1 What Are They?; 1.3.2 Problems; 1.3.3 Stable Ribozymes; 1.3.4 Designing Ribozymes; 1.4 Ribozymes or Antisense DNAs?; 1.5 The Choice Today!!; CHAPTER 2. Design and Synthesis of Antisense DNA Molecules; 2.1 Introduction; 2.2 Synthesis of Methylphosphonodiester-Phosphodiester Chimeric Oligodeoxynucleotides
2.2.1 Materials and Chemicals2.2.2 Solutions; 2.2.3 Maximizing Product Purity; 2.2.4 Deprotection of Chimeric Oligodeoxynucleotides; 2.2.5 Failed Sequences; 2.3 Primary Purification by Reversed-Phase, Solid-Phase Extraction on C18 SEP-PAK Cartridges; 2.3.1 Equipment; 2.3.2 Method; 2.3.3 Purification of the Oligodeoxynucleotide; 2.3.4 Further Purification; 2.4 Analysis and Purification by HPLC; 2.4.1 Analysis of Chimeric Oligodeoxynucleotides by HPLC; 2.4.2 Purification of Chimeric Oligodeoxynucleotides by HPLC; 2.4.3 Re-Use of Columns 2.5 Synthesis of Chimeric Oligodeoxynucleotides with Fluorescein Attached2.6 Summary; CHAPTER 3. The Design and Synthesis of Hammerhead Ribozymes; 3.1 Introduction; 3.2 The Design of Hammerhead Ribozymes; 3.3 Improving the Reactions; 3.3.1 Accessibility of the Target - Substrate Binding; 3.3.2 Finding the Target; 3.3.3 Theoretical Considerations; 3.3.4 Experimental Approaches; 3.3.5 Kinetic Studies; 3.4 Length of Arms; 3.4.1 Choosing Antisense Arms of Hammerhead Ribozymes; 3.4.2 Arms of Different Lengths; 3.5 Cleavage of the Target Motif; 3.6 Synthesis of Ribozymes 3.6.1 Chemical Synthesis of Short Hammerhead Ribozymes3.6.2 Enzymatic Transcription in Vitro; 3.7 Endogenous Expression of Ribozyme Genes; CHAPTER 4. Delivery of Ribozymes and Antisense DNA Molecules into Mammalian Cells; 4.1 Introduction; 4.2 Exogenous Application; 4.2.1 lntracytoplasmic Delivery of Antisense Oligodeoxynucleotides by Reversible Plasma Membrane Permeabilization with Streptolysin O; 4.3 Microinjection; 4.4 Other Methods Used in Nucleic Acid Transfection; 4.5 Electroporation; 4.5.1 Method; 4.5.2 Transfection: Optimization of Conditions 4.5.3 Mechanism of Uptake Following Electroporation4.5.4 Benefits and Drawbacks of Electrophoretic-Mediated Uptake; 4.6 Diethylaminoethyl-Dextran (DEAE- Dextran) and DNA Transfection; 4.6.1 Methods for Transfection of Adherent Cells; 4.6.2 Possible Alterations of the Above Protocol; 4.6.3 Transfection of Cells Growing in Suspension; 4.6.4 Transfection Optimization; 4.6.5 Distribution Mechanism of DEAE-Dextran Uptake and lntracellular; 4.6.6 Benefits and Drawbacks; 4.7 Calcium Phosphate Transfection; 4.7.1 Method; 4.7.2 Possible Alterations to Above Method; 4.7.3 Method Optimization 4.7.4 Calcium Phosphate-Mediated Uptake and lntracellular Distribution |
| Record Nr. | UNISA-996203927503316 |
| London, [England] : , : Chapman & Hall, , 1997 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
Antisense and ribozyme methodology / / Ian Gibson, editor
| Antisense and ribozyme methodology / / Ian Gibson, editor |
| Pubbl/distr/stampa | London, [England] : , : Chapman & Hall, , 1997 |
| Descrizione fisica | 1 online resource (96 p.) |
| Disciplina |
572.88
615.36 |
| Collana | Laboratory Companion Series |
| Soggetto topico |
Antisense nucleic acids
Catalytic RNA |
| ISBN |
1-281-84239-7
9786611842390 3-527-61253-X 3-527-61252-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Antisense and Ribozyme Methodology; Contents; CHAPTER 1. Antisense and Ribozyme Methodology; 1.1 The Potential; 1.2 Antisense Technology; 1.2.1 Problems; 1.2.2 Resistance to Nucleases; 1.2.3 Entry into Cells; 1.2.4 How Antisense Works; 1.2.5 Success; 1.3 Ribozymes; 1.3.1 What Are They?; 1.3.2 Problems; 1.3.3 Stable Ribozymes; 1.3.4 Designing Ribozymes; 1.4 Ribozymes or Antisense DNAs?; 1.5 The Choice Today!!; CHAPTER 2. Design and Synthesis of Antisense DNA Molecules; 2.1 Introduction; 2.2 Synthesis of Methylphosphonodiester-Phosphodiester Chimeric Oligodeoxynucleotides
2.2.1 Materials and Chemicals2.2.2 Solutions; 2.2.3 Maximizing Product Purity; 2.2.4 Deprotection of Chimeric Oligodeoxynucleotides; 2.2.5 Failed Sequences; 2.3 Primary Purification by Reversed-Phase, Solid-Phase Extraction on C18 SEP-PAK Cartridges; 2.3.1 Equipment; 2.3.2 Method; 2.3.3 Purification of the Oligodeoxynucleotide; 2.3.4 Further Purification; 2.4 Analysis and Purification by HPLC; 2.4.1 Analysis of Chimeric Oligodeoxynucleotides by HPLC; 2.4.2 Purification of Chimeric Oligodeoxynucleotides by HPLC; 2.4.3 Re-Use of Columns 2.5 Synthesis of Chimeric Oligodeoxynucleotides with Fluorescein Attached2.6 Summary; CHAPTER 3. The Design and Synthesis of Hammerhead Ribozymes; 3.1 Introduction; 3.2 The Design of Hammerhead Ribozymes; 3.3 Improving the Reactions; 3.3.1 Accessibility of the Target - Substrate Binding; 3.3.2 Finding the Target; 3.3.3 Theoretical Considerations; 3.3.4 Experimental Approaches; 3.3.5 Kinetic Studies; 3.4 Length of Arms; 3.4.1 Choosing Antisense Arms of Hammerhead Ribozymes; 3.4.2 Arms of Different Lengths; 3.5 Cleavage of the Target Motif; 3.6 Synthesis of Ribozymes 3.6.1 Chemical Synthesis of Short Hammerhead Ribozymes3.6.2 Enzymatic Transcription in Vitro; 3.7 Endogenous Expression of Ribozyme Genes; CHAPTER 4. Delivery of Ribozymes and Antisense DNA Molecules into Mammalian Cells; 4.1 Introduction; 4.2 Exogenous Application; 4.2.1 lntracytoplasmic Delivery of Antisense Oligodeoxynucleotides by Reversible Plasma Membrane Permeabilization with Streptolysin O; 4.3 Microinjection; 4.4 Other Methods Used in Nucleic Acid Transfection; 4.5 Electroporation; 4.5.1 Method; 4.5.2 Transfection: Optimization of Conditions 4.5.3 Mechanism of Uptake Following Electroporation4.5.4 Benefits and Drawbacks of Electrophoretic-Mediated Uptake; 4.6 Diethylaminoethyl-Dextran (DEAE- Dextran) and DNA Transfection; 4.6.1 Methods for Transfection of Adherent Cells; 4.6.2 Possible Alterations of the Above Protocol; 4.6.3 Transfection of Cells Growing in Suspension; 4.6.4 Transfection Optimization; 4.6.5 Distribution Mechanism of DEAE-Dextran Uptake and lntracellular; 4.6.6 Benefits and Drawbacks; 4.7 Calcium Phosphate Transfection; 4.7.1 Method; 4.7.2 Possible Alterations to Above Method; 4.7.3 Method Optimization 4.7.4 Calcium Phosphate-Mediated Uptake and lntracellular Distribution |
| Record Nr. | UNINA-9910830630503321 |
| London, [England] : , : Chapman & Hall, , 1997 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||