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Biochemistry laboratory manual for undergraduates : an inquiry-based approach / / Timea Gerczei, Scott Pattison; managing editor, Anna Rulka
| Biochemistry laboratory manual for undergraduates : an inquiry-based approach / / Timea Gerczei, Scott Pattison; managing editor, Anna Rulka |
| Autore | Gerczei Timea |
| Pubbl/distr/stampa | Warsaw, [Poland] ; ; Berlin, [Germany] : , : De Gruyter Open, , 2014 |
| Descrizione fisica | 1 online resource (186 pages) : illustrations, (some colour) |
| Disciplina | 572.078 |
| Soggetto topico |
Biochemistry
Molecular biology Drug resistance in microorganisms |
| ISBN |
1-5231-0070-2
3-11-042625-0 3-11-041133-4 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Front matter -- Preface -- Contents -- 1 Introducing the Bacterial Antibiotic Sensor Mini Project -- 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function -- 3 Designing Primers for Site-Directed Mutagenesis -- 4 Performing Site-Directed Mutagenesis -- 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy -- 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease -- 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro -- 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry -- 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching -- 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy -- 11 Understanding the Importance of Buffers in Biological Systems -- 12 Molecular Visualization of an Enzyme, Acetylcholinesterase -- 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment -- 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC -- List of Figures |
| Record Nr. | UNINA-9910132278903321 |
Gerczei Timea
|
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| Warsaw, [Poland] ; ; Berlin, [Germany] : , : De Gruyter Open, , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Biochemistry laboratory manual for undergraduates : an inquiry-based approach / / Timea Gerczei, Scott Pattison; managing editor, Anna Rulka
| Biochemistry laboratory manual for undergraduates : an inquiry-based approach / / Timea Gerczei, Scott Pattison; managing editor, Anna Rulka |
| Autore | Gerczei Timea |
| Pubbl/distr/stampa | Warsaw, [Poland] ; ; Berlin, [Germany] : , : De Gruyter Open, , 2014 |
| Descrizione fisica | 1 online resource (186 pages) : illustrations, (some colour) |
| Disciplina | 572.078 |
| Soggetto topico |
Biochemistry
Molecular biology Drug resistance in microorganisms |
| ISBN |
1-5231-0070-2
3-11-042625-0 3-11-041133-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Front matter -- Preface -- Contents -- 1 Introducing the Bacterial Antibiotic Sensor Mini Project -- 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function -- 3 Designing Primers for Site-Directed Mutagenesis -- 4 Performing Site-Directed Mutagenesis -- 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy -- 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease -- 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro -- 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry -- 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching -- 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy -- 11 Understanding the Importance of Buffers in Biological Systems -- 12 Molecular Visualization of an Enzyme, Acetylcholinesterase -- 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment -- 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC -- List of Figures |
| Record Nr. | UNISA-996308751403316 |
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Gerczei Timea
|
||
| Warsaw, [Poland] ; ; Berlin, [Germany] : , : De Gruyter Open, , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||