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Culture of animal cells : a manual of basic technique and specialized applications / / R. Ian Freshney ; reviewing editors, Amanda Capes-Davis, Carl Gregory, Stefan Przyborski
Culture of animal cells : a manual of basic technique and specialized applications / / R. Ian Freshney ; reviewing editors, Amanda Capes-Davis, Carl Gregory, Stefan Przyborski
Autore Freshney R. Ian
Edizione [Seventh edition.]
Pubbl/distr/stampa Hoboken, New Jersey : , : Wiley-Blackwell, , 2016
Descrizione fisica 1 online resource (758 p.)
Disciplina 571.6/381
Soggetto topico Tissue culture
Cell culture
Soggetto genere / forma Electronic books.
ISBN 1-118-87337-8
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Intro -- Titlepage -- Copyright -- Dedication -- List of Color Plates -- List of Protocols -- List of Minireviews -- Preface and Acknowledgments -- Abbreviations -- About the Companion Website -- CHAPTER 1 Introduction -- 1.1 HISTORICAL BACKGROUND -- 1.2 ADVANTAGES OF TISSUE CULTURE -- 1.3 LIMITATIONS -- 1.4 MAJOR DIFFERENCES IN VITRO -- 1.5 TYPES OF TISSUE CULTURE -- REFERENCES -- CHAPTER 2 Biology of Cultured Cells -- 2.1 THE CULTURE ENVIRONMENT -- 2.2 CELL ADHESION -- 2.3 CELL PROLIFERATION -- 2.4 DIFFERENTIATION -- 2.5 CELL SIGNALING -- 2.6 ENERGY METABOLISM -- 2.7 ORIGIN OF CULTURED CELLS -- 2.8 DEFINITIONS -- REFERENCES -- CHAPTER 3 Laboratory Design and Layout -- 3.1 PLANNING, FURNISHING, AND SERVICES -- 3.2 DESIGN AND LAYOUT -- 3.3 DISASTER MANAGEMENT -- REFERENCES -- CHAPTER 4 Equipment and Materials -- 4.1 REQUIREMENTS OF A TISSUE CULTURE LABORATORY -- 4.2 STERILE WORKING AREA -- 4.3 INCUBATION AND CULTURE -- 4.4 PREPARATION AND STERILIZATION -- 4.5 STORAGE -- 4.6 SUPPLEMENTARY LABORATORY EQUIPMENT -- 4.7 SPECIALIZED EQUIPMENT -- REFERENCES -- CHAPTER 5 Aseptic Technique -- 5.1 OBJECTIVES OF ASEPTIC TECHNIQUE -- 5.2 ELEMENTS OF ASEPTIC ENVIRONMENT -- 5.3 STERILE HANDLING -- 5.4 STANDARD PROCEDURE -- 5.5 APPARATUS AND EQUIPMENT -- REFERENCES -- CHAPTER 6 Safety, Bioethics, and Validation -- 6.1 LABORATORY SAFETY -- 6.2 RISK ASSESSMENT -- 6.3 STANDARD OPERATING PROCEDURES -- 6.4 SAFETY REGULATIONS -- 6.5 GENERAL SAFETY -- 6.6 FIRE -- 6.7 IONIZING RADIATION -- 6.8 BIOHAZARDS -- 6.9 BIOETHICS -- 6.10 QUALITY ASSURANCE -- REFERENCES -- CHAPTER 7 Culture Vessels and Substrates -- 7.1 THE SUBSTRATE -- 7.2 TREATED SURFACES -- 7.3 CHOICE OF CULTURE VESSEL -- 7.4 SPECIALIZED SYSTEMS -- REFERENCES -- CHAPTER 8 Defined Media and Supplements -- 8.1 DEVELOPMENT OF MEDIA -- 8.2 PHYSICOCHEMICAL PROPERTIES -- 8.3 BALANCED SALT SOLUTIONS.
8.4 COMPLETE MEDIA -- 8.5 SERUM -- 8.6 SELECTION OF MEDIUM AND SERUM -- 8.7 OTHER SUPPLEMENTS -- 8.8 STORAGE -- REFERENCES -- CHAPTER 9 Serum-Free Media -- 9.1 DISADVANTAGES OF SERUM -- 9.2 ADVANTAGES OF SERUM-FREE MEDIA -- 9.3 DISADVANTAGES OF SERUM-FREE MEDIA -- 9.4 REPLACEMENT OF SERUM -- 9.5 DEVELOPMENT OF SERUM-FREE MEDIUM -- 9.6 SELECTION OF SERUM-FREE MEDIUM -- 9.7 PREPARATION OF SERUM-FREE MEDIUM -- 9.8 ANIMAL PROTEIN−FREE MEDIA -- 9.9 CONCLUSIONS -- REFERENCES -- CHAPTER 10 Preparation and Sterilization -- 10.1 PREPARATION OF REAGENTS AND MATERIALS -- 10.2 STERILIZATION OF APPARATUS AND LIQUIDS -- 10.3 APPARATUS -- 10.4 REAGENTS AND MEDIA -- 10.5 STERILIZATION OF MEDIA -- 10.6 CONTROL, TESTING, AND STORAGE OF MEDIA -- REFERENCES -- CHAPTER 11 Primary Culture -- 11.1 INITIATION OF A PRIMARY CELL CULTURE -- 11.2 ISOLATION OF THE TISSUE -- 11.3 TYPES OF PRIMARY CULTURE -- REFERENCES -- CHAPTER 12 Subculture and Cell Lines -- 12.1 TERMINOLOGY -- 12.2 SUBCULTURE AND PROPAGATION -- 12.3 CHOOSING A CELL LINE -- 12.4 ROUTINE MAINTENANCE -- 12.5 SUBCULTURE -- REFERENCES -- CHAPTER 13 Authentication and Validation -- 13.1 AUTHENTICATION OF CELL LINES -- 13.2 VALIDATION -- 13.3 QUALITY ASSURANCE -- REFERENCES -- CHAPTER 14 Microbial Contamination -- 14.1 SOURCES OF CONTAMINATION -- 14.2 TYPES OF MICROBIAL CONTAMINATION -- 14.3 MONITORING FOR CONTAMINATION -- 14.4 DISPOSAL OF CONTAMINATED CULTURES -- 14.5 ERADICATION OF CONTAMINATION -- 14.6 CROSS-CONTAMINATION -- 14.7 CONCLUSIONS -- REFERENCES -- CHAPTER 15 Cryopreservation and Banking -- 15.1 RATIONALE FOR FREEZING -- 15.2 CONSIDERATIONS BEFORE CRYOPRESERVATION -- 15.3 PRINCIPLES OF CRYOPRESERVATION -- 15.4 DESIGN AND CONTROL OF FREEZER STOCKS -- 15.5 CELL BANKS -- 15.6 TRANSPORTING CELLS -- REFERENCES -- CHAPTER 16 Cloning and Selection -- 16.1 CELL CLONING -- 16.3 SUSPENSION CLONING.
16.4 ISOLATION OF CLONES -- 16.5 REPLICA PLATING -- 16.6 SELECTIVE MEDIA AND INHIBITORS -- 16.7 INTERACTION WITH SUBSTRATE -- REFERENCES -- CHAPTER 17 Cell Sorting -- 17.1 CELL DENSITY AND ISOPYKNIC SEDIMENTATION -- 17.2 CELL SIZE AND SEDIMENTATION VELOCITY -- 17.3 ANTIBODY-BASED TECHNIQUES -- 17.4 BEGINNER'S APPROACH TO CELL SEPARATION -- REFERENCES -- CHAPTER 18 Cell Line Characterization -- 18.1 THE NEED FOR CHARACTERIZATION -- 18.2 GENOTYPIC CHARACTERIZATION -- 18.3 PHENOTYPIC CHARACTERIZATION -- 18.4 CELL MORPHOLOGY -- 18.5 ANTIGENIC MARKERS -- 18.6 TIME-LAPSE RECORDING -- 18.7 CELL CYCLE -- REFERENCES -- CHAPTER 19 Differentiation -- 19.1 EXPRESSION OF THE IN VIVO PHENOTYPE -- 19.2 STAGES OF DIFFERENTIATION -- 19.3 PROLIFERATION AND DIFFERENTIATION -- 19.4 COMMITMENT AND LINEAGE -- 19.5 STEM CELL PLASTICITY -- 19.6 MARKERS OF DIFFERENTIATION -- 19.7 INDUCTION OF DIFFERENTIATION -- 19.8 DIFFERENTIATION AND MALIGNANCY -- 19.9 PRACTICAL ASPECTS -- REFERENCES -- CHAPTER 20 Three-Dimensional Culture -- 20.1 CELL INTERACTION AND PHENOTYPIC EXPRESSION -- 20.2 ORGAN CULTURE -- 20.3 HISTOTYPIC CULTURE -- 20.4 ORGANOTYPIC CULTURE -- 20.5 IMAGING CELLS IN 3D CONSTRUCTS -- REFERENCES -- CHAPTER 21 Scale-up and Automation -- 21.1 SCALE-UP IN SUSPENSION -- 21.2 SCALE-UP IN MONOLAYER -- 21.3 PROCESS CONTROL -- 21.4 LARGE-SCALE PROCESSES -- 21.5 AUTOMATION -- REFERENCES -- CHAPTER 22 Senescence, Immortalization, and Transformation -- 22.1 ROLE IN CELL LINE CHARACTERIZATION -- 22.2 GENETIC INSTABILITY AND HETEROGENEITY -- 22.3 IMMORTALIZATION -- 22.4 ABERRANT GROWTH CONTROL -- 22.5 TUMORIGENICITY -- REFERENCES -- CHAPTER 23 Quantitation -- 23.1 CELL COUNTING -- 23.2 CELL WEIGHT AND PACKED CELL VOLUME -- 23.3 DNA CONTENT -- 23.4 PROTEIN -- 23.5 RATES OF SYNTHESIS -- 23.6 PREPARATION OF SAMPLES FOR ENZYME ASSAY AND IMMUNOASSAY -- 23.7 CYTOMETRY.
23.8 REPLICATE SAMPLING -- 23.9 CELL PROLIFERATION -- 23.10 PLATING EFFICIENCY -- 23.11 LABELING INDEX -- 23.12 AUTORADIOGRAPHY -- 23.13 CELL CYCLE TIME -- 23.14 CELL MIGRATION -- REFERENCES -- CHAPTER 24 Cytotoxicity -- 24.1 VIABILITY, TOXICITY, AND SURVIVAL -- 24.2 IN VITRO LIMITATIONS -- 24.3 NATURE OF THE ASSAY -- 24.4 APPLICATIONS OF CYTOTOXICITY ASSAYS -- 24.5 GENOTOXICITY -- 24.6 INFLAMMATION -- REFERENCES -- CHAPTER 25 Culture of Specific Cell Types -- 25.1 CELL CULTURE OF SPECIALIZED CELLS -- 25.2 EPITHELIAL CELLS -- 25.3 MESENCHYMAL CELLS -- 25.4 NEUROECTODERMAL CELLS -- 25.5 HEMATOPOIETIC CELLS -- 25.6 PRODUCTION OF MONOCLONAL ANTIBODIES -- 25.7 GONADS -- 25.8 CULTURE OF CELLS FROM POIKILOTHERMS -- 25.9 TUMOR CELL CULTURE -- 25.10 SAMPLING -- 25.11 DISAGGREGATION -- 25.12 PRIMARY CULTURE -- 25.13 SELECTIVE CULTURE OF TUMOR CELLS -- 25.14 DEVELOPMENT OF CELL LINES -- 25.15 CHARACTERIZATION OF TUMOR CELL CULTURES -- 25.16 SPECIFIC TUMOR TYPES -- REFERENCES -- CHAPTER 26 Stem Cells -- 26.1 EMBRYONIC STEM CELLS -- 26.2 GERM CELLS -- 26.3 EXTRAEMBRYONIC CELLS -- 26.4 HEMOPOIETIC STEM CELLS -- 26.5 APPICATION OF STEM CELLS TO REGENERATIVE MEDICINE -- 26.6 CANCER STEM CELLS -- REFERENCES -- CHAPTER 27 Training Programs -- 27.1 OBJECTIVES -- 27.2 PREPARATIVE AND MANIPULATIVE SKILLS -- 27.3 BASIC CELL CULTURE TECHNIQUES -- 27.4 ADVANCED EXERCISES -- 27.5 SPECIALIZED EXERCISES -- REFERENCES -- CHAPTER 28 Problem Solving -- 28.1 ABNORMAL APPEARANCE OF CELLS -- 28.2 SLOW CELL GROWTH -- 28.3 MEDIUM -- 28.4 SUBSTRATES AND CONTAINERS -- 28.5 MICROBIAL CONTAMINATION -- 28.6 CHEMICAL CONTAMINATION -- 28.7 PRIMARY CULTURE -- 28.8 DIFFERENTIATION -- 28.9 FEEDING -- 28.10 SUBCULTURE -- 28.11 CLONING -- 28.12 CROSS-CONTAMINATION AND MISIDENTIFICATION -- 28.13 CRYOPRESERVATION -- 28.14 CELL COUNTING -- REFERENCE -- CHAPTER 29 In Conclusion.
Appendix I Calculations and preparation of reagents -- REFERENCES -- APPENDIX II Glossary -- REFERENCE -- Index -- Links -- Supplementary Images -- EULA.
Record Nr. UNINA-9910460932803321
Freshney R. Ian  
Hoboken, New Jersey : , : Wiley-Blackwell, , 2016
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Culture of animal cells : a manual of basic technique and specialized applications / / R. Ian Freshney ; reviewing editors, Amanda Capes-Davis, Carl Gregory, Stefan Przyborski
Culture of animal cells : a manual of basic technique and specialized applications / / R. Ian Freshney ; reviewing editors, Amanda Capes-Davis, Carl Gregory, Stefan Przyborski
Autore Freshney R. Ian
Edizione [Seventh edition.]
Pubbl/distr/stampa Hoboken, New Jersey : , : Wiley-Blackwell, , 2016
Descrizione fisica 1 online resource (758 pages, 30 unnumbered pages of plates) : illustrations
Disciplina 571.6/381
Soggetto topico Cell culture
Tissue culture
ISBN 9781118873373
1118873378
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910796100103321
Freshney R. Ian  
Hoboken, New Jersey : , : Wiley-Blackwell, , 2016
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Culture of animal cells : a manual of basic technique and specialized applications / / R. Ian Freshney ; reviewing editors, Amanda Capes-Davis, Carl Gregory, Stefan Przyborski
Culture of animal cells : a manual of basic technique and specialized applications / / R. Ian Freshney ; reviewing editors, Amanda Capes-Davis, Carl Gregory, Stefan Przyborski
Autore Freshney R. Ian
Edizione [Seventh edition.]
Pubbl/distr/stampa Hoboken, New Jersey : , : Wiley-Blackwell, , 2016
Descrizione fisica 1 online resource (758 pages, 30 unnumbered pages of plates) : illustrations
Disciplina 571.6/381
Soggetto topico Cell culture
Tissue culture
ISBN 9781118873373
1118873378
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910820130403321
Freshney R. Ian  
Hoboken, New Jersey : , : Wiley-Blackwell, , 2016
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Culture of animal cells : a manual of basic technique and specialized applications / / R. Ian Freshney
Culture of animal cells : a manual of basic technique and specialized applications / / R. Ian Freshney
Autore Freshney R. Ian
Edizione [6th ed.]
Pubbl/distr/stampa Hoboken, N.J., : Wiley-Blackwell, c2010
Descrizione fisica 1 online resource (766 p.)
Disciplina 571.6/381
571.6381
Soggetto topico Tissue culture
Cell culture
ISBN 1-283-02488-8
9786613024886
0-470-64936-4
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Culture of Animal Cells; Contents; List of Figures; List of Color Plates; List of Protocols; Preface and Acknowledgements; Abbreviations; 1. Introduction; 1.1. Historical Background; 1.2. Advantages of Tissue Culture; 1.2.1. Control of the Environment; 1.2.2. Characterization and Homogeneity of Samples; 1.2.3. Economy, Scale, and Mechanization; 1.2.4. In vitro Modeling of In vivo Conditions; 1.3. Limitations; 1.3.1. Expertise; 1.3.2. Quantity; 1.3.3. Dedifferentiation and Selection; 1.3.4. Origin of Cells; 1.3.5. Instability; 1.4. Major Differences In vitro; 1.5. Types of Tissue Culture
2. Biology of Cultured Cells2.1. The Culture Environment; 2.2. Cell Adhesion; 2.2.1. Cell Adhesion Molecules; 2.2.2. Intercellular Junctions; 2.2.3. Extracellular Matrix; 2.2.4. Cytoskeleton; 2.2.5. Cell Motility; 2.3. Cell Proliferation; 2.3.1. Cell Cycle; 2.3.2. Control of Cell Proliferation; 2.4. Differentiation; 2.4.1. Maintenance of Differentiation; 2.4.2. Dedifferentiation; 2.5. Cell Signaling; 2.6. Energy Metabolism; 2.7. Origin of Cultured Cells; 2.7.1. Initiation of the Culture; 2.7.2. Evolution of Cell Lines; 2.7.3. Senescence
2.7.4. Transformation and the Development of Continuous Cell Lines3. Laboratory Design, Layout, and Equipment; 3.1. Layout, Furnishing, and Services; 3.1.1. Requirements; 3.1.2. Services; 3.1.3. Ventilation; 3.2. Layout; 3.2.1. Sterile Handling Area; 3.2.2. Laminar Flow; 3.2.3. Service Bench; 3.2.4. Quarantine and Containment; 3.2.5. Incubation; 3.2.6. Preparation Area; 3.2.7. Storage; 4. Equipment and Materials; 4.1. Requirements of a Tissue Culture Laboratory; 4.2. Aseptic Area; 4.2.1. Laminar-Flow Hood; 4.2.2. Service Carts; 4.2.3. Sterile Liquid Handling-Pipetting and Dispensing
4.2.4. Inverted Microscope4.2.5. CCD Camera and Monitor; 4.2.6. Dissecting Microscope; 4.2.7. Centrifuge; 4.2.8. Cell Counting; 4.3. Incubation and Culture; 4.3.1. Incubator; 4.3.2. Humid CO2 Incubator; 4.3.3. Temperature Recorder; 4.3.4. Roller Racks; 4.3.5. Magnetic Stirrer; 4.3.6. Culture Vessels; 4.4. Preparation and Sterilization; 4.4.1. Washup; 4.4.2. Preparation of Media and Reagents; 4.4.3. Sterilization; 4.5. Storage; 4.5.1. Consumables; 4.5.2. Refrigerators and Freezers; 4.5.3. Cryostorage Containers; 4.5.4. Controlled-Rate Freezer; 4.6. Supplementary Laboratory Equipment
4.6.1. Computers and Networks4.6.2. Upright Microscope; 4.6.3. Low-Temperature Freezer; 4.6.4. Confocal Microscope; 4.6.5. PCR Thermal Cycler; 4.7. Specialized Equipment; 4.7.1. Microinjection Facilities; 4.7.2. Colony Counter; 4.7.3. Centrifugal Elutriator; 4.7.4. Flow Cytometer; 5. Aseptic Technique; 5.1. Objectives of Aseptic Technique; 5.1.1. Risk of Contamination; 5.1.2. Maintaining Sterility; 5.2. Elements of Aseptic Environment; 5.2.1. Laminar Flow; 5.2.2. Quiet Area; 5.2.3. Work Surface; 5.2.4. Personal Hygiene; 5.2.5. Reagents and Media; 5.2.6. Cultures; 5.3. Sterile Handling
5.3.1. Swabbing
Record Nr. UNINA-9910133601903321
Freshney R. Ian  
Hoboken, N.J., : Wiley-Blackwell, c2010
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Culture of animal cells : a manual of basic technique
Culture of animal cells : a manual of basic technique
Autore Freshney R. Ian
Pubbl/distr/stampa [Place of publication not identified], : Wiley Liss, 2005
Disciplina 571.6/381
Soggetto topico Culture Techniques
Cells, Cultured
Clinical Laboratory Techniques
Cells
Investigative Techniques
Anatomy
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Cytology
Biology
Health & Biological Sciences
ISBN 0-471-73990-1
0-471-74759-9
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910143953503321
Freshney R. Ian  
[Place of publication not identified], : Wiley Liss, 2005
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Culture of animal cells : a manual of basic technique
Culture of animal cells : a manual of basic technique
Autore Freshney R. Ian
Pubbl/distr/stampa [Place of publication not identified], : Wiley Liss, 2005
Disciplina 571.6/381
Soggetto topico Culture Techniques
Cells, Cultured
Clinical Laboratory Techniques
Cells
Investigative Techniques
Anatomy
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Cytology
Biology
Health & Biological Sciences
ISBN 0-471-73990-1
0-471-74759-9
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910677802603321
Freshney R. Ian  
[Place of publication not identified], : Wiley Liss, 2005
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui