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Genetic manipulation : techniques and applications / / edited by J.M. Grange, A. Fox & N.L. Morgan
| Genetic manipulation : techniques and applications / / edited by J.M. Grange, A. Fox & N.L. Morgan |
| Pubbl/distr/stampa | Oxford ; ; Boston, : Blackwell Scientific Publications, 1991 |
| Descrizione fisica | 1 online resource (417 p.) |
| Disciplina |
575.10724
660/.65 |
| Altri autori (Persone) |
GrangeJohn M
FoxA <1927-> (Arnold) MorganN. L <1951-> (Neil L.) |
| Collana | The Society for Applied Bacteriology technical series |
| Soggetto topico |
Genetic engineering
Biotechnology |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-282-23726-8
9786612237263 1-4443-1414-9 1-4443-1413-0 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Genetic Manipulation: Techniques and Applications; Contents; Contributors; Preface; Extraction, Purification and Assay of DNA; Isolation of DNA; Isolation of Plasmid DNA from Bacteria; Purification of DNA; Isolating Genomic DNA From Bacterial Cells; Isolating Mammalian DNA; Assay of DNA; References; Restriction Mapping of DNA; Restriction Enzymes; Approaches to Mapping; References; The Construction and Use of Cloning Vectors; The Commercially Available Vectors; The Construction of Vectors for Use with the Clostridia; References; Appendix I: Addresses of Suppliers of Cloning Vectors
Appendix II: Addresses of Suppliers of Other Products Useful for Molecular BiologyAdaptor Based cDNA Cloning in the Phage Vectors lgt10 and lgt11; Experimental Methods; Results of cDNA Library Analysis; Discussion; Acknowledgements; References; Plasmid Profiling and DNA/DNA Hybridization for Distinguishing Between Mesophilic Aeromonas Bacteria; DNA/DNA Hybridization; Plasmid Profiling; Methods; Results; Acknowledgements; References; Appendix: Reagents; Preparation and Screening of Bacterial Genomic Libraries; General Strategy; Construction of a Genomic Library by using lEMBL4 Screening of a lEMBL4 Library with Gene ProbesConstruction of a lgt11 Library and Screening with Antibodies; Acknowledgements; References; DNA Probes for Detection and Identification of Bacteria; Principle of Nucleic Acid Hybridization; Application of DNA Hybridization; The Polymerase Chain Reaction; References; Extraction and Purification of Eukaryotic mRNA; Basic Precautions Prior to RNA Extraction; RNA Extraction Protocols; Analysis of Purified RNA; Isolation of Polyadenylated mRNA from Total RNA; Storage of RNA Samples; Acknowledgements; References; cDNA Cloning How Many Clones are Required?Cloning Strategies; First-strand Synthesis; Second-strand Synthesis; Preparation of the cDNA for Cloning; Methylation of the cDNA; Linker Kinase Reaction; Test of Ligation Efficiency; Ligation of the Phosphorylated Linkers to the cDNA; EcoRI Digestion and Removal of Excess Linkers from the cDNA; Size Fractionation of the Linkered cDNA; Ligating Fragments into pUC Vectors; Transformation of Host Bacteria; Screening for Recombinant Phages and Plasmids; References; Primer Extension Sequencing of RNA Viruses; Precautions when Handling RNA Nucleotide Sequencing of Influenza Virus GenesPrimer Extension Sequencing of Influenza A Viruses; Primer Extension Sequencing of Flavivirus Genes; Comments; References; Appendix: Buffers and Reagents; Forensic Applications of DNA Profiling; MuItiIocus Probes; Single Locus Probes; Methods; DNA Profiling in Forensic Casework; References; Application of Nucleic Acid Probes to the Identification of Bacterial Enteric Pathogens; Identification of Enterotoxigenic Escherichia coli from Faecal Specimens; Gene Probes for other Bacterial Enteric Pathogens Prospects for Nucleic Acid Probes in Clinical Microbiology |
| Record Nr. | UNINA-9910139924503321 |
| Oxford ; ; Boston, : Blackwell Scientific Publications, 1991 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Genetic manipulation : techniques and applications / / edited by J.M. Grange, A. Fox & N.L. Morgan
| Genetic manipulation : techniques and applications / / edited by J.M. Grange, A. Fox & N.L. Morgan |
| Pubbl/distr/stampa | Oxford ; ; Boston, : Blackwell Scientific Publications, 1991 |
| Descrizione fisica | 1 online resource (417 p.) |
| Disciplina |
575.10724
660/.65 |
| Altri autori (Persone) |
GrangeJohn M
FoxA <1927-> (Arnold) MorganN. L <1951-> (Neil L.) |
| Collana | The Society for Applied Bacteriology technical series |
| Soggetto topico |
Genetic engineering
Biotechnology |
| ISBN |
1-282-23726-8
9786612237263 1-4443-1414-9 1-4443-1413-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Genetic Manipulation: Techniques and Applications; Contents; Contributors; Preface; Extraction, Purification and Assay of DNA; Isolation of DNA; Isolation of Plasmid DNA from Bacteria; Purification of DNA; Isolating Genomic DNA From Bacterial Cells; Isolating Mammalian DNA; Assay of DNA; References; Restriction Mapping of DNA; Restriction Enzymes; Approaches to Mapping; References; The Construction and Use of Cloning Vectors; The Commercially Available Vectors; The Construction of Vectors for Use with the Clostridia; References; Appendix I: Addresses of Suppliers of Cloning Vectors
Appendix II: Addresses of Suppliers of Other Products Useful for Molecular BiologyAdaptor Based cDNA Cloning in the Phage Vectors lgt10 and lgt11; Experimental Methods; Results of cDNA Library Analysis; Discussion; Acknowledgements; References; Plasmid Profiling and DNA/DNA Hybridization for Distinguishing Between Mesophilic Aeromonas Bacteria; DNA/DNA Hybridization; Plasmid Profiling; Methods; Results; Acknowledgements; References; Appendix: Reagents; Preparation and Screening of Bacterial Genomic Libraries; General Strategy; Construction of a Genomic Library by using lEMBL4 Screening of a lEMBL4 Library with Gene ProbesConstruction of a lgt11 Library and Screening with Antibodies; Acknowledgements; References; DNA Probes for Detection and Identification of Bacteria; Principle of Nucleic Acid Hybridization; Application of DNA Hybridization; The Polymerase Chain Reaction; References; Extraction and Purification of Eukaryotic mRNA; Basic Precautions Prior to RNA Extraction; RNA Extraction Protocols; Analysis of Purified RNA; Isolation of Polyadenylated mRNA from Total RNA; Storage of RNA Samples; Acknowledgements; References; cDNA Cloning How Many Clones are Required?Cloning Strategies; First-strand Synthesis; Second-strand Synthesis; Preparation of the cDNA for Cloning; Methylation of the cDNA; Linker Kinase Reaction; Test of Ligation Efficiency; Ligation of the Phosphorylated Linkers to the cDNA; EcoRI Digestion and Removal of Excess Linkers from the cDNA; Size Fractionation of the Linkered cDNA; Ligating Fragments into pUC Vectors; Transformation of Host Bacteria; Screening for Recombinant Phages and Plasmids; References; Primer Extension Sequencing of RNA Viruses; Precautions when Handling RNA Nucleotide Sequencing of Influenza Virus GenesPrimer Extension Sequencing of Influenza A Viruses; Primer Extension Sequencing of Flavivirus Genes; Comments; References; Appendix: Buffers and Reagents; Forensic Applications of DNA Profiling; MuItiIocus Probes; Single Locus Probes; Methods; DNA Profiling in Forensic Casework; References; Application of Nucleic Acid Probes to the Identification of Bacterial Enteric Pathogens; Identification of Enterotoxigenic Escherichia coli from Faecal Specimens; Gene Probes for other Bacterial Enteric Pathogens Prospects for Nucleic Acid Probes in Clinical Microbiology |
| Record Nr. | UNISA-996200224203316 |
| Oxford ; ; Boston, : Blackwell Scientific Publications, 1991 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
Genetic manipulation : techniques and applications / / edited by J.M. Grange, A. Fox & N.L. Morgan
| Genetic manipulation : techniques and applications / / edited by J.M. Grange, A. Fox & N.L. Morgan |
| Pubbl/distr/stampa | Oxford ; ; Boston, : Blackwell Scientific Publications, 1991 |
| Descrizione fisica | 1 online resource (417 p.) |
| Disciplina |
575.10724
660/.65 |
| Altri autori (Persone) |
GrangeJohn M
FoxA <1927-> (Arnold) MorganN. L <1951-> (Neil L.) |
| Collana | The Society for Applied Bacteriology technical series |
| Soggetto topico |
Genetic engineering
Biotechnology |
| ISBN |
1-282-23726-8
9786612237263 1-4443-1414-9 1-4443-1413-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Genetic Manipulation: Techniques and Applications; Contents; Contributors; Preface; Extraction, Purification and Assay of DNA; Isolation of DNA; Isolation of Plasmid DNA from Bacteria; Purification of DNA; Isolating Genomic DNA From Bacterial Cells; Isolating Mammalian DNA; Assay of DNA; References; Restriction Mapping of DNA; Restriction Enzymes; Approaches to Mapping; References; The Construction and Use of Cloning Vectors; The Commercially Available Vectors; The Construction of Vectors for Use with the Clostridia; References; Appendix I: Addresses of Suppliers of Cloning Vectors
Appendix II: Addresses of Suppliers of Other Products Useful for Molecular BiologyAdaptor Based cDNA Cloning in the Phage Vectors lgt10 and lgt11; Experimental Methods; Results of cDNA Library Analysis; Discussion; Acknowledgements; References; Plasmid Profiling and DNA/DNA Hybridization for Distinguishing Between Mesophilic Aeromonas Bacteria; DNA/DNA Hybridization; Plasmid Profiling; Methods; Results; Acknowledgements; References; Appendix: Reagents; Preparation and Screening of Bacterial Genomic Libraries; General Strategy; Construction of a Genomic Library by using lEMBL4 Screening of a lEMBL4 Library with Gene ProbesConstruction of a lgt11 Library and Screening with Antibodies; Acknowledgements; References; DNA Probes for Detection and Identification of Bacteria; Principle of Nucleic Acid Hybridization; Application of DNA Hybridization; The Polymerase Chain Reaction; References; Extraction and Purification of Eukaryotic mRNA; Basic Precautions Prior to RNA Extraction; RNA Extraction Protocols; Analysis of Purified RNA; Isolation of Polyadenylated mRNA from Total RNA; Storage of RNA Samples; Acknowledgements; References; cDNA Cloning How Many Clones are Required?Cloning Strategies; First-strand Synthesis; Second-strand Synthesis; Preparation of the cDNA for Cloning; Methylation of the cDNA; Linker Kinase Reaction; Test of Ligation Efficiency; Ligation of the Phosphorylated Linkers to the cDNA; EcoRI Digestion and Removal of Excess Linkers from the cDNA; Size Fractionation of the Linkered cDNA; Ligating Fragments into pUC Vectors; Transformation of Host Bacteria; Screening for Recombinant Phages and Plasmids; References; Primer Extension Sequencing of RNA Viruses; Precautions when Handling RNA Nucleotide Sequencing of Influenza Virus GenesPrimer Extension Sequencing of Influenza A Viruses; Primer Extension Sequencing of Flavivirus Genes; Comments; References; Appendix: Buffers and Reagents; Forensic Applications of DNA Profiling; MuItiIocus Probes; Single Locus Probes; Methods; DNA Profiling in Forensic Casework; References; Application of Nucleic Acid Probes to the Identification of Bacterial Enteric Pathogens; Identification of Enterotoxigenic Escherichia coli from Faecal Specimens; Gene Probes for other Bacterial Enteric Pathogens Prospects for Nucleic Acid Probes in Clinical Microbiology |
| Record Nr. | UNINA-9910830959103321 |
| Oxford ; ; Boston, : Blackwell Scientific Publications, 1991 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Genetic manipulation : techniques and applications / / edited by J.M. Grange, A. Fox & N.L. Morgan
| Genetic manipulation : techniques and applications / / edited by J.M. Grange, A. Fox & N.L. Morgan |
| Pubbl/distr/stampa | Oxford ; ; Boston, : Blackwell Scientific Publications, 1991 |
| Descrizione fisica | 1 online resource (417 p.) |
| Disciplina |
575.10724
660/.65 |
| Altri autori (Persone) |
GrangeJohn M
FoxA <1927-> (Arnold) MorganN. L <1951-> (Neil L.) |
| Collana | The Society for Applied Bacteriology technical series |
| Soggetto topico |
Genetic engineering
Biotechnology |
| ISBN |
1-282-23726-8
9786612237263 1-4443-1414-9 1-4443-1413-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Genetic Manipulation: Techniques and Applications; Contents; Contributors; Preface; Extraction, Purification and Assay of DNA; Isolation of DNA; Isolation of Plasmid DNA from Bacteria; Purification of DNA; Isolating Genomic DNA From Bacterial Cells; Isolating Mammalian DNA; Assay of DNA; References; Restriction Mapping of DNA; Restriction Enzymes; Approaches to Mapping; References; The Construction and Use of Cloning Vectors; The Commercially Available Vectors; The Construction of Vectors for Use with the Clostridia; References; Appendix I: Addresses of Suppliers of Cloning Vectors
Appendix II: Addresses of Suppliers of Other Products Useful for Molecular BiologyAdaptor Based cDNA Cloning in the Phage Vectors lgt10 and lgt11; Experimental Methods; Results of cDNA Library Analysis; Discussion; Acknowledgements; References; Plasmid Profiling and DNA/DNA Hybridization for Distinguishing Between Mesophilic Aeromonas Bacteria; DNA/DNA Hybridization; Plasmid Profiling; Methods; Results; Acknowledgements; References; Appendix: Reagents; Preparation and Screening of Bacterial Genomic Libraries; General Strategy; Construction of a Genomic Library by using lEMBL4 Screening of a lEMBL4 Library with Gene ProbesConstruction of a lgt11 Library and Screening with Antibodies; Acknowledgements; References; DNA Probes for Detection and Identification of Bacteria; Principle of Nucleic Acid Hybridization; Application of DNA Hybridization; The Polymerase Chain Reaction; References; Extraction and Purification of Eukaryotic mRNA; Basic Precautions Prior to RNA Extraction; RNA Extraction Protocols; Analysis of Purified RNA; Isolation of Polyadenylated mRNA from Total RNA; Storage of RNA Samples; Acknowledgements; References; cDNA Cloning How Many Clones are Required?Cloning Strategies; First-strand Synthesis; Second-strand Synthesis; Preparation of the cDNA for Cloning; Methylation of the cDNA; Linker Kinase Reaction; Test of Ligation Efficiency; Ligation of the Phosphorylated Linkers to the cDNA; EcoRI Digestion and Removal of Excess Linkers from the cDNA; Size Fractionation of the Linkered cDNA; Ligating Fragments into pUC Vectors; Transformation of Host Bacteria; Screening for Recombinant Phages and Plasmids; References; Primer Extension Sequencing of RNA Viruses; Precautions when Handling RNA Nucleotide Sequencing of Influenza Virus GenesPrimer Extension Sequencing of Influenza A Viruses; Primer Extension Sequencing of Flavivirus Genes; Comments; References; Appendix: Buffers and Reagents; Forensic Applications of DNA Profiling; MuItiIocus Probes; Single Locus Probes; Methods; DNA Profiling in Forensic Casework; References; Application of Nucleic Acid Probes to the Identification of Bacterial Enteric Pathogens; Identification of Enterotoxigenic Escherichia coli from Faecal Specimens; Gene Probes for other Bacterial Enteric Pathogens Prospects for Nucleic Acid Probes in Clinical Microbiology |
| Record Nr. | UNINA-9910877789103321 |
| Oxford ; ; Boston, : Blackwell Scientific Publications, 1991 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||