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Genetic analysis [[electronic resource] ] : principles, scope, and objectives / / John R.S. Fincham
| Genetic analysis [[electronic resource] ] : principles, scope, and objectives / / John R.S. Fincham |
| Autore | Fincham J. R. S |
| Pubbl/distr/stampa | Oxford ; ; Boston, : Blackwell Scientific Publications, 1994 |
| Descrizione fisica | 1 online resource (241 p.) |
| Disciplina | 574.87/322 |
| Soggetto topico |
Genetics - Technique
Nucleotide sequence Gene mapping |
| ISBN |
1-282-18905-0
9786612189050 1-4443-1383-5 1-4443-1382-7 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
GENETIC ANALYSIS; CONTENTS; PREFACE; INTRODUCTION: THE EXPANDING SCOPE OF GENETICS; 1: DISSECTING THE GENOME USING NATURAL GENETIC SYSTEMS; The eukaryotic system; Eukaryotes distinguished from prokaryotes; Mitosis and chromosome structure; Alternation of haploid and diploid phases in the sexual cycle; Meiosis and the rules of classical genetics; Meiosis; Single-factor genetic ratios and their explanation; Independent assortment of different allele-pairs; Testing data for fit to theoretical ratios; Linkage groups and the nature of crossovers; Map units and map distance
Placing Linked genes in sequenceDistinguishing between independent assorment and distant linkage; Mapping centromeres using tetrads; Sex linkage; Segregation and linkage in human genetics; Assigning linkage groups to chromosomes; Genetic analysis of bacteria and bacteriophage; Three modes of gene transfer in bacteria; Time-of-entry mapping in E. coli; Analogy with sexual recombination and segregation; Stable partial diploids - F' plasmids; Bacteriophage lambda - a virus in the chromosome; Bacteriophage T4 - a model recombinational system; Eukaryotic organelle genetics Exceptions to the rule of equal results from reciprocal crossesChloroplast variants; Mitochondrial variants; Summary and perspective; Further reading; References; 2: FROM MUTATIONS TO GENES; Defining the gene by mutation and complementation; What is it that the markers mark?; The collection of mutants; Sorting of mutants into complementation groups; An interim definition of the gene; Mapping within the gene; The detection of recombination within genes; Mapping by reference to flanking markers; Recombination frequency; Deletion mapping; The determination by genes of protein structure Colinearity of the gene and its encoded protein productBiochemistry of polypeptide synthesis; Not all genes encode proteins; Conclusions; References; 3: THE GENE AS DNA SEQUENCE; Characterization of DNA fragments; Restriction endonucleases; Separating aod sizing DNA fragments; Probing for specific sequences; Making restriction-site maps; Cloning and cloning vectors; Making recombinant DNA molecules; Escherichia coli plasmid vectors; Yeast 2-um plasmid and shuttle vectors; Lambda (l) bacteriophage and cosmid vectors; Yeast artificial chromosomes (YACs) Screening DNA libraries for functional genesGenomic libraries and cDNA libraries; Gene identification by complementation of mutants; Screening gene libraries with DNA probes; Designing gene-specific probes; Use of cDNA probes; Screening for gene expression; Probing across species or group boundaries; Positional cloning; Confirming the identity of cloned sequences; DNA sequence and open reading frames; Using the clone to disrupt the corresponding gene; Hybridization back to chromosomes; Simultaneous mutagenesis and gene tagging; The principle; Transposon tags; Conclusion; References 4: THE EVOLVING CONCEPT OF THE GENE |
| Record Nr. | UNISA-996206293403316 |
Fincham J. R. S
|
||
| Oxford ; ; Boston, : Blackwell Scientific Publications, 1994 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
Genetic analysis : principles, scope, and objectives / / John R.S. Fincham
| Genetic analysis : principles, scope, and objectives / / John R.S. Fincham |
| Autore | Fincham J. R. S |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Oxford ; ; Boston, : Blackwell Scientific Publications, 1994 |
| Descrizione fisica | 1 online resource (241 p.) |
| Disciplina | 574.87/322 |
| Soggetto topico |
Genetics - Technique
Nucleotide sequence Gene mapping |
| ISBN |
1-282-18905-0
9786612189050 1-4443-1383-5 1-4443-1382-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
GENETIC ANALYSIS; CONTENTS; PREFACE; INTRODUCTION: THE EXPANDING SCOPE OF GENETICS; 1: DISSECTING THE GENOME USING NATURAL GENETIC SYSTEMS; The eukaryotic system; Eukaryotes distinguished from prokaryotes; Mitosis and chromosome structure; Alternation of haploid and diploid phases in the sexual cycle; Meiosis and the rules of classical genetics; Meiosis; Single-factor genetic ratios and their explanation; Independent assortment of different allele-pairs; Testing data for fit to theoretical ratios; Linkage groups and the nature of crossovers; Map units and map distance
Placing Linked genes in sequenceDistinguishing between independent assorment and distant linkage; Mapping centromeres using tetrads; Sex linkage; Segregation and linkage in human genetics; Assigning linkage groups to chromosomes; Genetic analysis of bacteria and bacteriophage; Three modes of gene transfer in bacteria; Time-of-entry mapping in E. coli; Analogy with sexual recombination and segregation; Stable partial diploids - F' plasmids; Bacteriophage lambda - a virus in the chromosome; Bacteriophage T4 - a model recombinational system; Eukaryotic organelle genetics Exceptions to the rule of equal results from reciprocal crossesChloroplast variants; Mitochondrial variants; Summary and perspective; Further reading; References; 2: FROM MUTATIONS TO GENES; Defining the gene by mutation and complementation; What is it that the markers mark?; The collection of mutants; Sorting of mutants into complementation groups; An interim definition of the gene; Mapping within the gene; The detection of recombination within genes; Mapping by reference to flanking markers; Recombination frequency; Deletion mapping; The determination by genes of protein structure Colinearity of the gene and its encoded protein productBiochemistry of polypeptide synthesis; Not all genes encode proteins; Conclusions; References; 3: THE GENE AS DNA SEQUENCE; Characterization of DNA fragments; Restriction endonucleases; Separating aod sizing DNA fragments; Probing for specific sequences; Making restriction-site maps; Cloning and cloning vectors; Making recombinant DNA molecules; Escherichia coli plasmid vectors; Yeast 2-um plasmid and shuttle vectors; Lambda (l) bacteriophage and cosmid vectors; Yeast artificial chromosomes (YACs) Screening DNA libraries for functional genesGenomic libraries and cDNA libraries; Gene identification by complementation of mutants; Screening gene libraries with DNA probes; Designing gene-specific probes; Use of cDNA probes; Screening for gene expression; Probing across species or group boundaries; Positional cloning; Confirming the identity of cloned sequences; DNA sequence and open reading frames; Using the clone to disrupt the corresponding gene; Hybridization back to chromosomes; Simultaneous mutagenesis and gene tagging; The principle; Transposon tags; Conclusion; References 4: THE EVOLVING CONCEPT OF THE GENE |
| Record Nr. | UNINA-9910139918503321 |
|
Fincham J. R. S
|
||
| Oxford ; ; Boston, : Blackwell Scientific Publications, 1994 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||