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RNA methodologies [[electronic resource] ] : a laboratory guide for isolation and characterization / / Robert E. Farrell, Jr
| RNA methodologies [[electronic resource] ] : a laboratory guide for isolation and characterization / / Robert E. Farrell, Jr |
| Autore | Farrell Robert E |
| Edizione | [3rd ed.] |
| Pubbl/distr/stampa | Amsterdam ; ; Boston, : Elsevier/Academic Press, c2005 |
| Descrizione fisica | 1 online resource (794 p.) |
| Disciplina | 572.8/8 |
| Soggetto topico |
RNA - Analysis
Nucleic acids - Analysis |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-280-62859-6
9786610628599 0-08-045476-3 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front cover; Title page; Copyright page; Dedication; Table of contents; Preface; 1 RNA and the Cellular Biochemistry Revisited; Why Study RNA?; What Is RNA?; Assembly of Polynucleotides; Types of RNA; Stringency: Conditions That Influence Nucleic Acid Structure; Types of Double-Stranded Molecules; References and Suggested Reading; 2 Transcription and the Organization of Eukaryotic Genes; Transcription and the Central Dogma; Gene Organization; RNA Polymerases and the Products of Transcription; References; 3 Messenger RNA; Topology of a Typical mRNA Molecule; Stability in the Cytoplasm
Levels of RegulationReferences; 4 Resilient Ribonucleases; Rationale; Elimination of Ribonuclease Activity; Types of Ribonuclease Inhibitors; Preparation of Equipment and Reagents; Other Reagents Used to Control Nuclease Activity; Protocol: Synthesis of Vanadyl Ribonucleoside Complexes; References; 5 RNA Isolation Strategies; Rationale; Goals in the Purification of RNA; Lysis Buffer Formulations; Isolation of RNA with Guanidinium Buffers; Guanidinium-Acid-Phenol Extraction Techniques; Density Gradient Centrifugation; Simultaneous Isolation of RNA and DNA; The Word on Kits; Other Methods Short- and Long-Term Storage of Purified RNAReferences; 6 The Truth About Tissues; Rationale; Tissue Culture or Tissue?; Homogenization Methods; RNA Isolation Strategies for Various Organs and Tissues; Protocol: LiCl-Urea Method for RNA Isolation from Tissue; Protocol: RNA Isolation from Lipid-Enriched Tissue; Purification of Polysome-Engaged mRNA; Collecting Samples in the Field; RNA ""Clean-Up"" Methods; References; 7 Isolation of Polyadenylated RNA; Rationale; Polyadenylation; The Poly(A) Caveat; Selection of Polyadenylated Molecules; Magnetic Bead Technology for Poly(A)+ Purification Oligo(dT)-Cellulose Column ChromatographyRapid, Non-Column Poly(A)+ Purification; References; 8 Quality Control for RNA Preparations; Rationale; Quality Control Technique 1: Electrophoretic Profile of the RNA; Quality Control Technique 2: Ultraviolet Spectrophotometry and Absorption Ratios; Quality Control Technique 3: Sample Capacity to Support RT-PCR; Quality Control Technique 4: Northern Analysis; Quality Control Technique 5: Sample Capacity to Support In Vitro Translation; References; 9 Dot Blot Analysis; Rationale; Advantages and Disadvantages; Appropriate Positive and Negative Controls Limitations of the DataReferences; 10 Electrophoresis of RNA; Rationale; Normalization of Nucleic Acids; RNA Denaturing Systems for Agarose Gel Electrophoresis; Molecular Weight Standards; Gel Staining Techniques; Safety Considerations in Electrophoresis; Maintenance of Electrophoresis Equipment; Running Agarose Gels for the First Time: A Few Tips; References; 11 Photodocumentation and Image Analysis; Rationale; Photodocumentation; Digital Image Analysis; Suggested Reading; 12 Northern Analysis; Rationale; Choice of Filter Membrane; Handling and Filter Preparation Northern Transfer Techniques |
| Record Nr. | UNINA-9910458669403321 |
Farrell Robert E
|
||
| Amsterdam ; ; Boston, : Elsevier/Academic Press, c2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
RNA methodologies [[electronic resource] ] : a laboratory guide for isolation and characterization / / Robert E. Farrell, Jr
| RNA methodologies [[electronic resource] ] : a laboratory guide for isolation and characterization / / Robert E. Farrell, Jr |
| Autore | Farrell Robert E |
| Edizione | [3rd ed.] |
| Pubbl/distr/stampa | Amsterdam ; ; Boston, : Elsevier/Academic Press, c2005 |
| Descrizione fisica | 1 online resource (794 p.) |
| Disciplina | 572.8/8 |
| Soggetto topico |
RNA - Analysis
Nucleic acids - Analysis |
| ISBN |
1-280-62859-6
9786610628599 0-08-045476-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front cover; Title page; Copyright page; Dedication; Table of contents; Preface; 1 RNA and the Cellular Biochemistry Revisited; Why Study RNA?; What Is RNA?; Assembly of Polynucleotides; Types of RNA; Stringency: Conditions That Influence Nucleic Acid Structure; Types of Double-Stranded Molecules; References and Suggested Reading; 2 Transcription and the Organization of Eukaryotic Genes; Transcription and the Central Dogma; Gene Organization; RNA Polymerases and the Products of Transcription; References; 3 Messenger RNA; Topology of a Typical mRNA Molecule; Stability in the Cytoplasm
Levels of RegulationReferences; 4 Resilient Ribonucleases; Rationale; Elimination of Ribonuclease Activity; Types of Ribonuclease Inhibitors; Preparation of Equipment and Reagents; Other Reagents Used to Control Nuclease Activity; Protocol: Synthesis of Vanadyl Ribonucleoside Complexes; References; 5 RNA Isolation Strategies; Rationale; Goals in the Purification of RNA; Lysis Buffer Formulations; Isolation of RNA with Guanidinium Buffers; Guanidinium-Acid-Phenol Extraction Techniques; Density Gradient Centrifugation; Simultaneous Isolation of RNA and DNA; The Word on Kits; Other Methods Short- and Long-Term Storage of Purified RNAReferences; 6 The Truth About Tissues; Rationale; Tissue Culture or Tissue?; Homogenization Methods; RNA Isolation Strategies for Various Organs and Tissues; Protocol: LiCl-Urea Method for RNA Isolation from Tissue; Protocol: RNA Isolation from Lipid-Enriched Tissue; Purification of Polysome-Engaged mRNA; Collecting Samples in the Field; RNA ""Clean-Up"" Methods; References; 7 Isolation of Polyadenylated RNA; Rationale; Polyadenylation; The Poly(A) Caveat; Selection of Polyadenylated Molecules; Magnetic Bead Technology for Poly(A)+ Purification Oligo(dT)-Cellulose Column ChromatographyRapid, Non-Column Poly(A)+ Purification; References; 8 Quality Control for RNA Preparations; Rationale; Quality Control Technique 1: Electrophoretic Profile of the RNA; Quality Control Technique 2: Ultraviolet Spectrophotometry and Absorption Ratios; Quality Control Technique 3: Sample Capacity to Support RT-PCR; Quality Control Technique 4: Northern Analysis; Quality Control Technique 5: Sample Capacity to Support In Vitro Translation; References; 9 Dot Blot Analysis; Rationale; Advantages and Disadvantages; Appropriate Positive and Negative Controls Limitations of the DataReferences; 10 Electrophoresis of RNA; Rationale; Normalization of Nucleic Acids; RNA Denaturing Systems for Agarose Gel Electrophoresis; Molecular Weight Standards; Gel Staining Techniques; Safety Considerations in Electrophoresis; Maintenance of Electrophoresis Equipment; Running Agarose Gels for the First Time: A Few Tips; References; 11 Photodocumentation and Image Analysis; Rationale; Photodocumentation; Digital Image Analysis; Suggested Reading; 12 Northern Analysis; Rationale; Choice of Filter Membrane; Handling and Filter Preparation Northern Transfer Techniques |
| Record Nr. | UNINA-9910784643203321 |
|
Farrell Robert E
|
||
| Amsterdam ; ; Boston, : Elsevier/Academic Press, c2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
RNA methodologies [[electronic resource] ] : a laboratory guide for isolation and characterization / / Robert E. Farrell, Jr
| RNA methodologies [[electronic resource] ] : a laboratory guide for isolation and characterization / / Robert E. Farrell, Jr |
| Autore | Farrell Robert E |
| Edizione | [3rd ed.] |
| Pubbl/distr/stampa | Amsterdam ; ; Boston, : Elsevier/Academic Press, c2005 |
| Descrizione fisica | 1 online resource (794 p.) |
| Disciplina | 572.8/8 |
| Soggetto topico |
RNA - Analysis
Nucleic acids - Analysis |
| ISBN |
1-280-62859-6
9786610628599 0-08-045476-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front cover; Title page; Copyright page; Dedication; Table of contents; Preface; 1 RNA and the Cellular Biochemistry Revisited; Why Study RNA?; What Is RNA?; Assembly of Polynucleotides; Types of RNA; Stringency: Conditions That Influence Nucleic Acid Structure; Types of Double-Stranded Molecules; References and Suggested Reading; 2 Transcription and the Organization of Eukaryotic Genes; Transcription and the Central Dogma; Gene Organization; RNA Polymerases and the Products of Transcription; References; 3 Messenger RNA; Topology of a Typical mRNA Molecule; Stability in the Cytoplasm
Levels of RegulationReferences; 4 Resilient Ribonucleases; Rationale; Elimination of Ribonuclease Activity; Types of Ribonuclease Inhibitors; Preparation of Equipment and Reagents; Other Reagents Used to Control Nuclease Activity; Protocol: Synthesis of Vanadyl Ribonucleoside Complexes; References; 5 RNA Isolation Strategies; Rationale; Goals in the Purification of RNA; Lysis Buffer Formulations; Isolation of RNA with Guanidinium Buffers; Guanidinium-Acid-Phenol Extraction Techniques; Density Gradient Centrifugation; Simultaneous Isolation of RNA and DNA; The Word on Kits; Other Methods Short- and Long-Term Storage of Purified RNAReferences; 6 The Truth About Tissues; Rationale; Tissue Culture or Tissue?; Homogenization Methods; RNA Isolation Strategies for Various Organs and Tissues; Protocol: LiCl-Urea Method for RNA Isolation from Tissue; Protocol: RNA Isolation from Lipid-Enriched Tissue; Purification of Polysome-Engaged mRNA; Collecting Samples in the Field; RNA ""Clean-Up"" Methods; References; 7 Isolation of Polyadenylated RNA; Rationale; Polyadenylation; The Poly(A) Caveat; Selection of Polyadenylated Molecules; Magnetic Bead Technology for Poly(A)+ Purification Oligo(dT)-Cellulose Column ChromatographyRapid, Non-Column Poly(A)+ Purification; References; 8 Quality Control for RNA Preparations; Rationale; Quality Control Technique 1: Electrophoretic Profile of the RNA; Quality Control Technique 2: Ultraviolet Spectrophotometry and Absorption Ratios; Quality Control Technique 3: Sample Capacity to Support RT-PCR; Quality Control Technique 4: Northern Analysis; Quality Control Technique 5: Sample Capacity to Support In Vitro Translation; References; 9 Dot Blot Analysis; Rationale; Advantages and Disadvantages; Appropriate Positive and Negative Controls Limitations of the DataReferences; 10 Electrophoresis of RNA; Rationale; Normalization of Nucleic Acids; RNA Denaturing Systems for Agarose Gel Electrophoresis; Molecular Weight Standards; Gel Staining Techniques; Safety Considerations in Electrophoresis; Maintenance of Electrophoresis Equipment; Running Agarose Gels for the First Time: A Few Tips; References; 11 Photodocumentation and Image Analysis; Rationale; Photodocumentation; Digital Image Analysis; Suggested Reading; 12 Northern Analysis; Rationale; Choice of Filter Membrane; Handling and Filter Preparation Northern Transfer Techniques |
| Record Nr. | UNINA-9910810775103321 |
|
Farrell Robert E
|
||
| Amsterdam ; ; Boston, : Elsevier/Academic Press, c2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||