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Flow cytometry with plant cells : analysis of genes, chromosomes and genomes / / edited by Jaroslav Dolezel, Johann Greilhuber, and Jan Suda
| Flow cytometry with plant cells : analysis of genes, chromosomes and genomes / / edited by Jaroslav Dolezel, Johann Greilhuber, and Jan Suda |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH |
| Descrizione fisica | 1 online resource (481 p.) |
| Disciplina | 571.62 |
| Altri autori (Persone) |
DolezelJaroslav
GreilhuberJohann SudaJan |
| Soggetto topico |
Flow cytometry
Plant cells and tissues |
| ISBN |
1-280-92167-6
9786610921676 3-527-61092-8 3-527-61093-6 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Flow Cytometry with Plant Cells; Contents; Preface; List of Contributors; 1 Cytometry and Cytometers: Development and Growth; Overview; 1.1 Origins; 1.2 From Absorption to Fluorescence, from Imaging to Flow; 1.2.1 Early Microspectrophotometry and Image Cytometry; 1.2.2 Fluorescence Microscopy and the Fluorescent Antibody Technique; 1.2.3 Computers Meet Cytometers: The Birth of Analytical Flow Cytometry; 1.2.4 The Development of Cell Sorting; 1.3 The Growth of Multiparameter Flow Cytometry; 1.4 Bench-tops and Behemoths: Convergent Evolution; 1.5 Image Cytometry: New Beginnings?; References
2 Principles of Flow CytometryOverview; 2.1 Introduction; 2.2 A Brief History of Flow Cytometry; 2.3 Components of a Flow Cytometer; 2.3.1 Fluidics; 2.3.2 Optics; 2.3.3 Electronic Systems; 2.4 Flow Cytometric Informatics; 2.5 Spectral Compensation; 2.6 Cell Sorting; 2.7 Calibration Issues; 2.8 Conclusions; References; 3 Flow Cytometry with Plants: an Overview; Overview; 3.1 Introduction; 3.2 Fluorescence is a Fundamental Parameter; 3.3 Pushing Plants through the Flow Cytometer; 3.3.1 Difficulties with Plants and their Cells; 3.3.2 Protoplasts are somewhat ""Easier"" than Intact Cells 3.3.3 Going for Organelles3.4 Application of Flow Cytometry in Plants; 3.4.1 Microspores and Pollen; 3.4.2 Protoplasts; 3.4.2.1 Physiological Processes; 3.4.2.2 Secondary Metabolites; 3.4.2.3 Gene Expression; 3.4.2.4 Somatic Hybrids; 3.4.2.5 DNA Transfection; 3.4.3 Cell Nuclei; 3.4.3.1 Ploidy Levels; 3.4.3.2 Aneuploidy; 3.4.3.3 B Chromosomes; 3.4.3.4 Sex Chromosomes; 3.4.3.5 Cell Cycle and Endopolyploidy; 3.4.3.6 Reproductive Pathways; 3.4.3.7 Nuclear Genome Size; 3.4.3.8 DNA Base Content; 3.4.3.9 Chromatin Composition; 3.4.3.10 Sorting of Nuclei; 3.4.4 Mitotic Chromosomes; 3.4.5 Chloroplasts 3.4.6 Mitochondria3.4.7 Plant Pathogens; 3.4.8 Aquatic Flow Cytometry; 3.5 A Flow Cytometer in Every Laboratory?; 3.6 Conclusions and Future Trends; References; 4 Nuclear DNA Content Measurement; Overview; 4.1 Introduction; 4.2 Nuclear DNA Content: Words, Concepts and Symbols; 4.2.1 Replication-Division Phases; 4.2.2 Alternation of Nuclear Phases; 4.2.3 Generative Polyploidy Levels; 4.2.4 Somatic Polyploidy; 4.3 Units for Presenting DNA Amounts and their Conversion Factors; 4.4 Sample Preparation for Flow Cytometric DNA Measurement; 4.4.1 Selection of the Tissue; 4.4.2 Reagents and Solutions 4.4.2.1 Isolation Buffers and DNA Staining4.5 Standardization; 4.5.1 Types of Standardization; 4.5.2 Requirement of Internal Standardization - a Practical Test; 4.5.3 Choice of the Appropriate Standard Species; 4.5.3.1 Biological Similarity; 4.5.3.2 Genome Size; 4.5.3.3 Nature of the Standard; 4.5.3.4 Availability; 4.5.3.5 Cytological Homogeneity; 4.5.3.6 Accessibility; 4.5.3.7 Reliability of C-Values; 4.5.4 Studies on Plant Standards; 4.5.5 Suggested Standards; 4.6 Fluorescence Inhibitors and Coatings of Debris; 4.6.1 What are Fluorescence Inhibitors and Coatings of Debris? 4.6.2 Experiments with Tannic Acid |
| Record Nr. | UNINA-9910877836603321 |
| Weinheim, : Wiley-VCH | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Plant genome diversity [[electronic resource] ] . Volume 2 : physical structure, behaviour and evolution of plant genomes / / Johann Greilhuber, Jaroslav Dolezel, Jonathan F. Wendel, editors
| Plant genome diversity [[electronic resource] ] . Volume 2 : physical structure, behaviour and evolution of plant genomes / / Johann Greilhuber, Jaroslav Dolezel, Jonathan F. Wendel, editors |
| Autore | Greilhuber Johann |
| Edizione | [1st ed. 2013.] |
| Pubbl/distr/stampa | Vienna, : Springer, 2013 |
| Descrizione fisica | 1 online resource (359 p.) |
| Disciplina |
572.8
572.8/62 572.862 |
| Altri autori (Persone) |
DolezelJaroslav
WendelJonathan F |
| Soggetto topico |
Plant genomes
Plant breeding |
| ISBN |
1-283-84959-3
3-7091-1160-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | 1. Angiosperm phylogeny: a framework for studies of genome evolution (P. S. Soltis, D. E. Soltis) -- 2. The plant nucleus at war and peace - genome organisation in the interphase nucleus (R. N. Jones, T. Langdon) -- 3. The organisation of genomic DNA in mitotic chromosomes: a novel view (H. Takata, S. Matsunaga, K. Maeshima) -- 4. Structural organisation of the plant nucleus: nuclear envelope, pore complexes and nucleoskeleton (E. Kiseleva, J. Fiserova, M. W. Goldberg).- 5. The plant nucleolus (P. Shaw) -- 6. Cell Cycle Modules in Plants for Entry Into Proliferation and for Mitosis (Z. Magyar, M. Ito, P. Binarová, B. Mohamed, L. Bogre).-7. Endopolyploidy in plants (J. Maluszynska, B. Kolano, H. Sas-Nowosielska) -- 8. Meiosis: recombination and the control of cell division (E. Jenczewski, R. Mercier, N. Macaisne, C. Mézard) -- 9. Mechanisms of chromosome rearrangements (M. A. Lysak, I. Schubert) -- 10. Biology and evolution of B chromosomes (A. Houben, A. M. B. Moghaddam, S. Klemme) -- 11. Chromosomes and Sex Differentiation (B. Janousek, R. Hobza, B. Vyskot) -- 12. Holocentric chromosomes (P. Bures, F. Zedek, M. Marková) -- 13. Karyotype diversity and evolutionary trends in angiosperms (H. Weiss-Schneeweiss, G. M. Schneeweiss) -- 14. Karyotype variation and evolution in gymnosperms (B. G. Murray) -- 15. Karyotype and Genome Evolution in Pteridophytes (M. S. Barker) -- 16. The incidence of polyploidy in natural plant populations: major patterns and evolutionary processes (B. C. Husband, S. J. Baldwin, J. Suda) -- 17. Significance and biological consequences of polyploidization in land plant evolution (J. A. Fawcett, Y. van de Peer, S. Maere) -- 18. Evolutionary importance of generative polyploidy for genome evolution of haploid-dominant land plants (S. A. Rensing, A. K. Beike, D. Lang) -- 19. Genome size diversity and evolution in land plants (I. J. Leitch, A. R. Leitch) -- 20. Genome size and the phenotype (J. Greilhuber, I. J. Leitch). |
| Record Nr. | UNINA-9910437841903321 |
Greilhuber Johann
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| Vienna, : Springer, 2013 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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