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Stem Cell Bioprocessing and Manufacturing
| Stem Cell Bioprocessing and Manufacturing |
| Autore | Cabral Joaquim M. S |
| Pubbl/distr/stampa | Basel, Switzerland, : MDPI - Multidisciplinary Digital Publishing Institute, 2020 |
| Descrizione fisica | 1 electronic resource (170 p.) |
| Soggetto topico | Medicine |
| Soggetto non controllato |
electrospinning
live-cell electrospinning tissue engineering cell seeding high voltage viability allogeneic cell therapy induced pluripotent stem cell human embryonic stem cell cell aggregate expansion differentiation scalable manufacturing scale up single-use bioreactor Vertical-Wheel U-shaped vessel computational fluid dynamics shear stress turbulent energy dissipation rates homogeneous hydrodynamic environment human pluripotent stem cells hepatic cell lineages hepatocyte differentiation non-parenchymal liver cells liver organoids disease modeling drug screening olfactory ensheathing cells spinal cord injury neural regeneration cell therapies adipose stem cells neurotrophic factors growth factors peripheral nerve injuries fibrin nerve conduits hydrogels stem cells delivery axonal regeneration Schwann cells stromal vascular fraction stem cell adipose-derived stem cell infrapatellar fat pad knee arthroscopy arthrotomy bioreactor hMSCs microcarrier bioprocess embryonic stem cells mesenchymal stromal cells blood platelets cell culture techniques progenitor cells human adipose stem cells (hASCs) serum- and xeno-free conditions UrSuppe stem cell culture medium autologous therapy kinetic growth modeling segregated and unstructured growth model model predictive control bio-process cell growth lactate advanced therapy medicinal products |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910557788003321 |
Cabral Joaquim M. S
|
||
| Basel, Switzerland, : MDPI - Multidisciplinary Digital Publishing Institute, 2020 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Stem Cell Manufacturing [[electronic resource]]
| Stem Cell Manufacturing [[electronic resource]] |
| Autore | Cabral Joaquim M. S |
| Pubbl/distr/stampa | Oxford, : Elsevier Science, 2015 |
| Descrizione fisica | 1 online resource (342 p.) |
| Disciplina | 616.02774 |
| Altri autori (Persone) |
da SilvaClaudia Lobato
ChaseLucas G DiogoMaria Margarida |
| Soggetto topico | Stem cells |
| ISBN |
0-444-63266-2
0-444-63265-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; STEM CELL MANUFACTURING; STEM CELL MANUFACTURING; Copyright; CONTENTS; LIST OF CONTRIBUTORS; INTRODUCTION; 1 - Genetic Engineering in Stem Cell Biomanufacturing ; 1.1 INTRODUCTION; 1.2 GENETIC MANIPULATION APPROACHES IN HUMAN PLURIPOTENT STEM CELLS; 1.2.1 Transgenic Approaches; 1.2.2 Knock-In and Knock-Out Approaches; 1.2.2.1 Zinc-Finger Nucleases; 1.2.2.2 Transcription Activator-Like Effector Nucleases; 1.2.2.3 Clustered Regularly Interspaced Short Palindromic Repeat/Cas9; 1.2.3 Bacterial Artificial Chromosomes; 1.3 APPLICATIONS
1.3.1 Genetic Labeling for Cell Identification and Cell Tracking1.3.2 Gene Alteration for Directed Differentiation; 1.3.3 Gene Disruption for Functional Investigation; 1.3.4 Gene Correction for Function Restoration; 1.4 DELIVERY METHODS; 1.4.1 Transfection; 1.4.2 Nucleofection; 1.4.3 Viral Transduction; 1.5 CONCLUSIONS; ACKNOWLEDGMENTS; REFERENCES; 2 - Biomechanics in Stem Cell Manufacturing; 2.1 INTRODUCTION; 2.2 CELLULAR BIOMECHANICS; 2.2.1 Biomechanical Cues; 2.2.2 Shear Forces and Differentiated Cells; 2.2.3 Shear Forces and Pluripotent Stem Cells 2.3 SCALE UP TOWARD PRODUCTION-LEVEL BIOREACTORS2.4 BIOMANUFACTURING CELLS FOR THERAPIES; 2.4.1 Pluripotent Stem Cells; 2.4.2 Cardiomyocytes; 2.4.3 Endothelial Cells; 2.5 CONCLUSION; REFERENCES; 3 - Bioreactor Engineering Fundamentals for Stem Cell Manufacturing ; 3.1 INTRODUCTION; 3.2 STIRRED BIOREACTOR BASICS; 3.3 SPECIAL FEATURES OF STIRRED BIOREACTORS FOR HMSC CULTURE ON MICROCARRIERS; 3.3.1 Introduction; 3.3.2 Preparing the Bioreactor for Culture; 3.3.3 Medium and Medium Exchange; 3.3.4 Microcarrier Selection; 3.3.5 Cell and Microcarrier Concentrations; 3.3.6 Attachment Protocol 3.3.7 Use of Coatings to Enhance Attachment3.3.8 The Minimum Speed for Suspension, NJS and Associated Mean Specific Energy Dissipation Rate, εT;̄ 3.3.8.1 General Aspects; 3.3.8.2 NJS Considerations in hMSC Culture; 3.3.9 Oxygen Demand, Mass Transfer, and Optimum Dissolved Oxygen; 3.3.9.1 General Considerations; 3.3.9.2 Application to hMSC Culture; 3.3.10 Fluid Dynamically Generated Stresses and Cell Proliferation; 3.3.10.1 General Considerations; 3.3.10.2 Application to hMSC Culture; 3.3.11 Fluid Dynamically Generated Stresses and Their Application to Cell Harvesting; 3.4 FUTURE ISSUES 3.4.1 Increasing Cell Density3.4.2 Oxygen Demand and Mass Transfer at Higher Cell Density-Sparging and Higher Agitator Speeds; 3.4.3 Carbon Dioxide, Osmolality, and pH; 3.4.4 Human-Induced and Embryonic Pluripotent Stem Cells; 3.5 CONCLUSIONS; NOMENCLATURE; REFERENCES; 4 - Microcarrier Culture Systems for Stem Cell Manufacturing ; 4.1 OVERVIEW; 4.1.1 Historical Perspective; 4.2 MICROCARRIER TECHNOLOGY; 4.2.1 Types of Microcarriers; 4.2.2 Properties of Microcarriers Required for Cell Culturing; 4.2.3 Advantages of Using Microcarrier Culture Systems for Cell Manufacturing 4.3 SCALABLE CULTURE SYSTEMS FOR ADHERENT STEM CELLS |
| Record Nr. | UNINA-9910798524903321 |
|
Cabral Joaquim M. S
|
||
| Oxford, : Elsevier Science, 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Stem Cell Manufacturing
| Stem Cell Manufacturing |
| Autore | Cabral Joaquim M. S |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Oxford, : Elsevier Science, 2015 |
| Descrizione fisica | 1 online resource (342 p.) |
| Disciplina | 616.02774 |
| Altri autori (Persone) |
da SilvaClaudia Lobato
ChaseLucas G DiogoMaria Margarida |
| Soggetto topico | Stem cells |
| ISBN |
0-444-63266-2
0-444-63265-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; STEM CELL MANUFACTURING; STEM CELL MANUFACTURING; Copyright; CONTENTS; LIST OF CONTRIBUTORS; INTRODUCTION; 1 - Genetic Engineering in Stem Cell Biomanufacturing ; 1.1 INTRODUCTION; 1.2 GENETIC MANIPULATION APPROACHES IN HUMAN PLURIPOTENT STEM CELLS; 1.2.1 Transgenic Approaches; 1.2.2 Knock-In and Knock-Out Approaches; 1.2.2.1 Zinc-Finger Nucleases; 1.2.2.2 Transcription Activator-Like Effector Nucleases; 1.2.2.3 Clustered Regularly Interspaced Short Palindromic Repeat/Cas9; 1.2.3 Bacterial Artificial Chromosomes; 1.3 APPLICATIONS
1.3.1 Genetic Labeling for Cell Identification and Cell Tracking1.3.2 Gene Alteration for Directed Differentiation; 1.3.3 Gene Disruption for Functional Investigation; 1.3.4 Gene Correction for Function Restoration; 1.4 DELIVERY METHODS; 1.4.1 Transfection; 1.4.2 Nucleofection; 1.4.3 Viral Transduction; 1.5 CONCLUSIONS; ACKNOWLEDGMENTS; REFERENCES; 2 - Biomechanics in Stem Cell Manufacturing; 2.1 INTRODUCTION; 2.2 CELLULAR BIOMECHANICS; 2.2.1 Biomechanical Cues; 2.2.2 Shear Forces and Differentiated Cells; 2.2.3 Shear Forces and Pluripotent Stem Cells 2.3 SCALE UP TOWARD PRODUCTION-LEVEL BIOREACTORS2.4 BIOMANUFACTURING CELLS FOR THERAPIES; 2.4.1 Pluripotent Stem Cells; 2.4.2 Cardiomyocytes; 2.4.3 Endothelial Cells; 2.5 CONCLUSION; REFERENCES; 3 - Bioreactor Engineering Fundamentals for Stem Cell Manufacturing ; 3.1 INTRODUCTION; 3.2 STIRRED BIOREACTOR BASICS; 3.3 SPECIAL FEATURES OF STIRRED BIOREACTORS FOR HMSC CULTURE ON MICROCARRIERS; 3.3.1 Introduction; 3.3.2 Preparing the Bioreactor for Culture; 3.3.3 Medium and Medium Exchange; 3.3.4 Microcarrier Selection; 3.3.5 Cell and Microcarrier Concentrations; 3.3.6 Attachment Protocol 3.3.7 Use of Coatings to Enhance Attachment3.3.8 The Minimum Speed for Suspension, NJS and Associated Mean Specific Energy Dissipation Rate, εT;̄ 3.3.8.1 General Aspects; 3.3.8.2 NJS Considerations in hMSC Culture; 3.3.9 Oxygen Demand, Mass Transfer, and Optimum Dissolved Oxygen; 3.3.9.1 General Considerations; 3.3.9.2 Application to hMSC Culture; 3.3.10 Fluid Dynamically Generated Stresses and Cell Proliferation; 3.3.10.1 General Considerations; 3.3.10.2 Application to hMSC Culture; 3.3.11 Fluid Dynamically Generated Stresses and Their Application to Cell Harvesting; 3.4 FUTURE ISSUES 3.4.1 Increasing Cell Density3.4.2 Oxygen Demand and Mass Transfer at Higher Cell Density-Sparging and Higher Agitator Speeds; 3.4.3 Carbon Dioxide, Osmolality, and pH; 3.4.4 Human-Induced and Embryonic Pluripotent Stem Cells; 3.5 CONCLUSIONS; NOMENCLATURE; REFERENCES; 4 - Microcarrier Culture Systems for Stem Cell Manufacturing ; 4.1 OVERVIEW; 4.1.1 Historical Perspective; 4.2 MICROCARRIER TECHNOLOGY; 4.2.1 Types of Microcarriers; 4.2.2 Properties of Microcarriers Required for Cell Culturing; 4.2.3 Advantages of Using Microcarrier Culture Systems for Cell Manufacturing 4.3 SCALABLE CULTURE SYSTEMS FOR ADHERENT STEM CELLS |
| Record Nr. | UNINA-9910814782103321 |
|
Cabral Joaquim M. S
|
||
| Oxford, : Elsevier Science, 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||