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Fundamentals of light microscopy and electronic imaging / / Douglas B. Murphy, Michael W. Davidson
Fundamentals of light microscopy and electronic imaging / / Douglas B. Murphy, Michael W. Davidson
Autore Murphy Douglas B
Edizione [2nd ed.]
Pubbl/distr/stampa Hoboken, N.J., : Wiley-Blackwell, 2012
Descrizione fisica 1 online resource (xiii, 538 p.)
Disciplina 502.8/2
Altri autori (Persone) DavidsonMichael W <1950-> (Michael Wesley)
Soggetto topico Microscòpia
Imatges - Processament - Tècniques digitals
Microscopy
Image processing - Digital techniques
ISBN 1-283-64427-4
1-118-38290-0
1-118-38291-9
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Title page; Copyright page; Contents; Preface; Acknowledgments; CHAPTER 1: Fundamentals of Light Microscopy; Overview; Optical Components of the Light Microscope; Aperture and Image Planes in a Focused, Adjusted Microscope; Koehler Illumination; Adjusting the Microscope for Koehler Illumination; Fixed Tube Length versus Infinity Optical Systems; Precautions for Handling Optical Equipment; Care and Maintenance of the Microscope; CHAPTER 2: Light and Color; Overview; Light as a Probe of Matter; The Dual Particle- and Wave-Like Nature of Light; The Quality of Light
Properties of Light Perceived by the EyePhysical Basis for Visual Perception and Color; Addition and Subtraction Colors; CHAPTER 3: Illuminators, Filters, and the Isolation of Specific Wavelengths; Overview; Illuminators and Their Spectra; Illuminator Alignment and Bulb Replacement; Filters for Adjusting the Intensity and Wavelength of Illumination; Neutral Density Filters; Colored Glass Filters; Interference Filters; Effects of Light on Living Cells; CHAPTER 4: Lenses and Geometrical Optics; Overview; Reflection and Refraction of Light; Image Formation by a Simple Lens
Rules of Ray Tracing for a Simple LensObject-Image Math; The Principal Aberrations of Lenses; Designs and Specifications of Objectives; Special Objective Designs; Markings on the Barrel of an Objective; Image Brightness; Objective Parfocality; Condensers; Oculars; Microscope Slides and Coverslips; The Care and Cleaning of Optics; Dust; Immersion Oil; Scratches and Abrasions; Mechanical Force; CHAPTER 5: Diffraction and Interference in Image Formation; Overview; Diffraction and Interference; The Diffraction Image of a Point Source of Light
The Constancy of Optical Path Length between Object and ImageEffect of Aperture Angle on Diffraction Spot Size; Diffraction by a Grating and Calculation of Its Line Spacing, D; Abbé's Theory for Image Formation in the Microscope; A Diffraction Pattern Is Formed in the Rear Aperture of the Objective; Preservation of Coherence: Essential Requirement for Image Formation; CHAPTER 6: Diffraction and Spatial Resolution; Overview; Numerical Aperture; Spatial Resolution; Depth of Field and Depth of Focus; Optimizing the Microscope Image: A Compromise between Spatial Resolution and Contrast
CHAPTER 7: Phase Contrast Microscopy and Darkfield MicroscopyOverview; Phase Contrast Microscopy; The Behavior of Waves from Phase Objects in Brightfield Microscopy; Wave Terminology and the Importance of Coherence; Depiction of Wave Interactions with Sine Wave and Vector Diagrams; The Role of Differences in Optical Path Lengths; The Optical Design of the Phase Contrast Microscope; Alignment; Interpreting the Phase Contrast Image; Darkfield Microscopy; Theory and Optics; Image Interpretation; CHAPTER 8: Properties of Polarized Light; Overview; The Generation of Polarized Light
Polarization by Reflection and Scattering
Record Nr. UNINA-9910141379403321
Murphy Douglas B  
Hoboken, N.J., : Wiley-Blackwell, 2012
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Microscopic Techniques for the Non-Expert / / edited by Sathish-Kumar Kamaraj, Arun Thirumurugan, Shanmuga Sundar Dhanabalan, Samuel A. Hevia
Microscopic Techniques for the Non-Expert / / edited by Sathish-Kumar Kamaraj, Arun Thirumurugan, Shanmuga Sundar Dhanabalan, Samuel A. Hevia
Edizione [1st ed. 2022.]
Pubbl/distr/stampa Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022
Descrizione fisica 1 online resource (251 pages)
Disciplina 578
Soggetto topico Medicine—Research
Biology—Research
Materials—Microscopy
Crystallography
Nanotechnology
Materials—Analysis
Imaging systems
Biomedical Research
Microscopy
Crystallography and Scattering Methods
Characterization and Analytical Technique
Imaging Techniques
Microscòpia
Manuals de laboratori
Soggetto genere / forma Llibres electrònics
ISBN 3-030-99542-9
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto A Beginner’s Guide to Different Types of Microscopes -- Principles of Light and Fluorescence Microscopy -- Confocal and Multiphoton Microscopy -- Scanning Probe Microscopy: tipping the path towards atomic visions -- Atomic Force Microscopy - An Advanced Imaging Technique: From Molecules to Morphologies -- Exploring the microcosm at atomic precision using Atomic Force Microscopy -- Scanning Electron Microscopy (SEM): Learning to generate and interpret the topographical aspects of materials -- Recent updates on methods, applications, and practical uses of scanning electron microscopy in various life sciences -- Transmission electron microscopy - A powerful and novel scientific technique with nanoscale resolution for characterization of materials -- Preparation of Biological Samples for SEM: Techniques and Procedures -- Index.
Record Nr. UNINA-9910735397703321
Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Optical imaging in human disease and biological research / / Xunbin Wei, Bobo Gu, editors
Optical imaging in human disease and biological research / / Xunbin Wei, Bobo Gu, editors
Pubbl/distr/stampa Singapore : , : Springer, , [2021]
Descrizione fisica 1 online resource (308 pages)
Disciplina 616.0754
Collana Advances in Experimental Medicine and Biology
Soggetto topico Diagnostic imaging
Imaging systems in biology
Microscopy
Diagnòstic per la imatge
Microscòpia
Soggetto genere / forma Llibres electrònics
ISBN 981-15-7627-0
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910483607803321
Singapore : , : Springer, , [2021]
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Principles of Light Microscopy: From Basic to Advanced / / edited by Volodymyr Nechyporuk-Zloy
Principles of Light Microscopy: From Basic to Advanced / / edited by Volodymyr Nechyporuk-Zloy
Edizione [1st ed. 2022.]
Pubbl/distr/stampa Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022
Descrizione fisica 1 online resource (327 pages)
Disciplina 578
570.282
Soggetto topico Medicine—Research
Biology—Research
Life sciences
Cytology
Spectrum analysis
Biomedical Research
Life Sciences
Cell Biology
Spectroscopy
Microscòpia
Soggetto genere / forma Llibres electrònics
ISBN 9783031044779
9783031044762
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Chapter 1. The physical principles of light propagation and light-matter interactions -- Chapter 2. Design, alignment and usage of infinity-corrected microscope -- Chapter 3. Epifluorescence Microscopy -- Chapter 4. Basic digital image design, processing, analysis, management, and presentation -- Chapter 5. Confocal Microscopy -- Chapter 6. Live-cell Imaging: A Balancing Act Between Speed, Sensitivity and Resolution -- Chapter 7. Structured Illumination Microscopy -- Chapter 8. Stimulated Emission Depletion Microscopy -- Chapter 9. Two-Photon Imaging -- Chapter 10. Modern microscopy image analysis: quantifying colocalization on a mobile device -- Chapter 11. Spinning Disk Microscopy -- Chapter 12. Light Sheet Fluorescence Microscopy -- Chapter 13. Localization microscopy: a review of the progress in methods and applications.
Record Nr. UNINA-9910633919403321
Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022
Materiale a stampa
Lo trovi qui: Univ. Federico II
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