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Fundamentals of light microscopy and electronic imaging / / Douglas B. Murphy, Michael W. Davidson
| Fundamentals of light microscopy and electronic imaging / / Douglas B. Murphy, Michael W. Davidson |
| Autore | Murphy Douglas B |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Blackwell, 2012 |
| Descrizione fisica | 1 online resource (xiii, 538 p.) |
| Disciplina | 502.8/2 |
| Altri autori (Persone) | DavidsonMichael W <1950-> (Michael Wesley) |
| Soggetto topico |
Microscòpia
Imatges - Processament - Tècniques digitals Microscopy Image processing - Digital techniques |
| ISBN |
1-283-64427-4
1-118-38290-0 1-118-38291-9 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Title page; Copyright page; Contents; Preface; Acknowledgments; CHAPTER 1: Fundamentals of Light Microscopy; Overview; Optical Components of the Light Microscope; Aperture and Image Planes in a Focused, Adjusted Microscope; Koehler Illumination; Adjusting the Microscope for Koehler Illumination; Fixed Tube Length versus Infinity Optical Systems; Precautions for Handling Optical Equipment; Care and Maintenance of the Microscope; CHAPTER 2: Light and Color; Overview; Light as a Probe of Matter; The Dual Particle- and Wave-Like Nature of Light; The Quality of Light
Properties of Light Perceived by the EyePhysical Basis for Visual Perception and Color; Addition and Subtraction Colors; CHAPTER 3: Illuminators, Filters, and the Isolation of Specific Wavelengths; Overview; Illuminators and Their Spectra; Illuminator Alignment and Bulb Replacement; Filters for Adjusting the Intensity and Wavelength of Illumination; Neutral Density Filters; Colored Glass Filters; Interference Filters; Effects of Light on Living Cells; CHAPTER 4: Lenses and Geometrical Optics; Overview; Reflection and Refraction of Light; Image Formation by a Simple Lens Rules of Ray Tracing for a Simple LensObject-Image Math; The Principal Aberrations of Lenses; Designs and Specifications of Objectives; Special Objective Designs; Markings on the Barrel of an Objective; Image Brightness; Objective Parfocality; Condensers; Oculars; Microscope Slides and Coverslips; The Care and Cleaning of Optics; Dust; Immersion Oil; Scratches and Abrasions; Mechanical Force; CHAPTER 5: Diffraction and Interference in Image Formation; Overview; Diffraction and Interference; The Diffraction Image of a Point Source of Light The Constancy of Optical Path Length between Object and ImageEffect of Aperture Angle on Diffraction Spot Size; Diffraction by a Grating and Calculation of Its Line Spacing, D; Abbé's Theory for Image Formation in the Microscope; A Diffraction Pattern Is Formed in the Rear Aperture of the Objective; Preservation of Coherence: Essential Requirement for Image Formation; CHAPTER 6: Diffraction and Spatial Resolution; Overview; Numerical Aperture; Spatial Resolution; Depth of Field and Depth of Focus; Optimizing the Microscope Image: A Compromise between Spatial Resolution and Contrast CHAPTER 7: Phase Contrast Microscopy and Darkfield MicroscopyOverview; Phase Contrast Microscopy; The Behavior of Waves from Phase Objects in Brightfield Microscopy; Wave Terminology and the Importance of Coherence; Depiction of Wave Interactions with Sine Wave and Vector Diagrams; The Role of Differences in Optical Path Lengths; The Optical Design of the Phase Contrast Microscope; Alignment; Interpreting the Phase Contrast Image; Darkfield Microscopy; Theory and Optics; Image Interpretation; CHAPTER 8: Properties of Polarized Light; Overview; The Generation of Polarized Light Polarization by Reflection and Scattering |
| Record Nr. | UNINA-9910141379403321 |
Murphy Douglas B
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| Hoboken, N.J., : Wiley-Blackwell, 2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Microscopic Techniques for the Non-Expert / / edited by Sathish-Kumar Kamaraj, Arun Thirumurugan, Shanmuga Sundar Dhanabalan, Samuel A. Hevia
| Microscopic Techniques for the Non-Expert / / edited by Sathish-Kumar Kamaraj, Arun Thirumurugan, Shanmuga Sundar Dhanabalan, Samuel A. Hevia |
| Edizione | [1st ed. 2022.] |
| Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 |
| Descrizione fisica | 1 online resource (251 pages) |
| Disciplina | 578 |
| Soggetto topico |
Medicine—Research
Biology—Research Materials—Microscopy Crystallography Nanotechnology Materials—Analysis Imaging systems Biomedical Research Microscopy Crystallography and Scattering Methods Characterization and Analytical Technique Imaging Techniques Microscòpia Manuals de laboratori |
| Soggetto genere / forma | Llibres electrònics |
| ISBN | 3-030-99542-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | A Beginner’s Guide to Different Types of Microscopes -- Principles of Light and Fluorescence Microscopy -- Confocal and Multiphoton Microscopy -- Scanning Probe Microscopy: tipping the path towards atomic visions -- Atomic Force Microscopy - An Advanced Imaging Technique: From Molecules to Morphologies -- Exploring the microcosm at atomic precision using Atomic Force Microscopy -- Scanning Electron Microscopy (SEM): Learning to generate and interpret the topographical aspects of materials -- Recent updates on methods, applications, and practical uses of scanning electron microscopy in various life sciences -- Transmission electron microscopy - A powerful and novel scientific technique with nanoscale resolution for characterization of materials -- Preparation of Biological Samples for SEM: Techniques and Procedures -- Index. |
| Record Nr. | UNINA-9910735397703321 |
| Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Optical imaging in human disease and biological research / / Xunbin Wei, Bobo Gu, editors
| Optical imaging in human disease and biological research / / Xunbin Wei, Bobo Gu, editors |
| Pubbl/distr/stampa | Singapore : , : Springer, , [2021] |
| Descrizione fisica | 1 online resource (308 pages) |
| Disciplina | 616.0754 |
| Collana | Advances in Experimental Medicine and Biology |
| Soggetto topico |
Diagnostic imaging
Imaging systems in biology Microscopy Diagnòstic per la imatge Microscòpia |
| Soggetto genere / forma | Llibres electrònics |
| ISBN | 981-15-7627-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910483607803321 |
| Singapore : , : Springer, , [2021] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Principles of Light Microscopy: From Basic to Advanced / / edited by Volodymyr Nechyporuk-Zloy
| Principles of Light Microscopy: From Basic to Advanced / / edited by Volodymyr Nechyporuk-Zloy |
| Edizione | [1st ed. 2022.] |
| Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 |
| Descrizione fisica | 1 online resource (327 pages) |
| Disciplina |
578
570.282 |
| Soggetto topico |
Medicine—Research
Biology—Research Life sciences Cytology Spectrum analysis Biomedical Research Life Sciences Cell Biology Spectroscopy Microscòpia |
| Soggetto genere / forma | Llibres electrònics |
| ISBN |
9783031044779
9783031044762 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Chapter 1. The physical principles of light propagation and light-matter interactions -- Chapter 2. Design, alignment and usage of infinity-corrected microscope -- Chapter 3. Epifluorescence Microscopy -- Chapter 4. Basic digital image design, processing, analysis, management, and presentation -- Chapter 5. Confocal Microscopy -- Chapter 6. Live-cell Imaging: A Balancing Act Between Speed, Sensitivity and Resolution -- Chapter 7. Structured Illumination Microscopy -- Chapter 8. Stimulated Emission Depletion Microscopy -- Chapter 9. Two-Photon Imaging -- Chapter 10. Modern microscopy image analysis: quantifying colocalization on a mobile device -- Chapter 11. Spinning Disk Microscopy -- Chapter 12. Light Sheet Fluorescence Microscopy -- Chapter 13. Localization microscopy: a review of the progress in methods and applications. |
| Record Nr. | UNINA-9910633919403321 |
| Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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