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Biophysical methods for biotherapeutics : discovery and development applications / / edited by Tapan K. Das
| Biophysical methods for biotherapeutics : discovery and development applications / / edited by Tapan K. Das |
| Pubbl/distr/stampa | Hoboken, New Jersey : , : John Wiley & Sons, , 2014 |
| Descrizione fisica | 1 online resource (381 p.) |
| Disciplina | 615.7 |
| Soggetto topico | Biopharmaceutics |
| ISBN |
1-118-35469-9
1-118-35467-2 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Biophysical Methods for Biotherapeutics; Contents; Preface; About the Editor; List of Contributors; Section 1 Early Discovery Stages and Biotherapeutic Candidate Selection; 1 Biophysical Methods Applied in Early Discovery of a Biotherapeutic: Case Study of an Egfr-Igf1r Bispecific Adnectin; 1.1 Introduction; 1.2 Target Identification; 1.3 Target Generation; 1.3.1 Multiple Constructs Strategy; 1.4 Hit Evaluation; 1.4.1 Qualitative and Rapid Self-Association Check; 1.4.2 Qualitative and Rapid Thermal Stability Check; 1.4.3 Confirmation of Binding; 1.5 Lead Selection; 1.5.1 Self-Association
1.5.2 Thermal Stability1.5.3 Binding Affinity, Kinetics, and Epitope; 1.6 Lead Optimization; 1.7 Lead Formatting; 1.7.1 Solubility; 1.7.2 Thermal Unfolding Behavior; 1.8 Final Development Candidate Selection; 1.9 Concluding Remarks; Acknowledgment; References; 2 X-ray Crystallography for Biotherapeutics; 2.1 Introduction to X-ray Crystallography; 2.1.1 Early X-Ray Crystallography for Biologics; 2.2 Modern X-ray Crystallography; 2.2.1 Construct Design and Protein Production; 2.2.2 Macromolecular Crystallization; 2.3 X-ray Data Collection; 2.3.1 Crystal Mounting; 2.3.2 Collecting a Data Set 2.3.3 Data Reduction2.4 Solving the Structure of the Crystal; 2.4.1 Molecular Replacement; 2.4.2 Heavy Atom Techniques; 2.4.3 Confirming the Validity of a Solution; 2.4.4 Building and Refining the Structure; 2.5 Understanding the Target Through Structure; 2.5.1 The Model; 2.5.2 The Protein Databank and Related Resources; 2.5.3 Information Provided by X-Ray Crystallography; 2.6 Applications of X-ray Crystallography to Biotherapeutics; 2.6.1 Antibody-Based Biotherapeutics; 2.6.2 Antibody Design; 2.6.3 Protein Receptor Interactions 2.7 Future Applications of Crystal Structures in Biotherapeutics2.7.1 Protein Engineering; 2.8 Conclusion; Acknowledgments; References; 3 Solubility and Early Assessment of Stability for Protein Therapeutics; 3.1 Introduction; 3.2 Measuring Protein Solubility; 3.2.1 Direct Measurement of Solubility: Concentration to Precipitation; 3.2.2 Indirect Assessment of Solubility: The Second Virial Coefficient (B22) and Self-Interaction Chromatography; 3.3 Assessment of Protein Stability; 3.3.1 Thermal Stability; 3.3.2 Aggregation; 3.3.3 Chemical Modifications; 3.4 Computational Predictions 3.4.1 Identifying Aggregation Promoting Regions3.4.2 Interaction Hot Spots; 3.5 Enhance the Solubility of Biotherapeutics; 3.5.1 Site-Directed Mutagenesis; 3.5.2 Pegylation; 3.5.3 Glycosylation; 3.5.4 Formulation Optimization; 3.6 Development of Rapid Methods to Identify Soluble and Stable Biotherapeutics; 3.7 Concluding Remarks; References; Section 2 First-in-Human and Up To Proof-of-Concept Clinical Trials; 4 Biophysical and Structural Characterization Needed Prior to Proof of Concept; 4.1 Introduction 4.2 Biophysical Methods for Elucidation of Protein Structure and Physiochemical Properties |
| Record Nr. | UNINA-9910140276203321 |
| Hoboken, New Jersey : , : John Wiley & Sons, , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Biophysical methods for biotherapeutics : discovery and development applications / / edited by Tapan K. Das
| Biophysical methods for biotherapeutics : discovery and development applications / / edited by Tapan K. Das |
| Pubbl/distr/stampa | Hoboken, New Jersey : , : John Wiley & Sons, , 2014 |
| Descrizione fisica | 1 online resource (381 p.) |
| Disciplina | 615.7 |
| Soggetto topico | Biopharmaceutics |
| ISBN |
1-118-35469-9
1-118-35467-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Biophysical Methods for Biotherapeutics; Contents; Preface; About the Editor; List of Contributors; Section 1 Early Discovery Stages and Biotherapeutic Candidate Selection; 1 Biophysical Methods Applied in Early Discovery of a Biotherapeutic: Case Study of an Egfr-Igf1r Bispecific Adnectin; 1.1 Introduction; 1.2 Target Identification; 1.3 Target Generation; 1.3.1 Multiple Constructs Strategy; 1.4 Hit Evaluation; 1.4.1 Qualitative and Rapid Self-Association Check; 1.4.2 Qualitative and Rapid Thermal Stability Check; 1.4.3 Confirmation of Binding; 1.5 Lead Selection; 1.5.1 Self-Association
1.5.2 Thermal Stability1.5.3 Binding Affinity, Kinetics, and Epitope; 1.6 Lead Optimization; 1.7 Lead Formatting; 1.7.1 Solubility; 1.7.2 Thermal Unfolding Behavior; 1.8 Final Development Candidate Selection; 1.9 Concluding Remarks; Acknowledgment; References; 2 X-ray Crystallography for Biotherapeutics; 2.1 Introduction to X-ray Crystallography; 2.1.1 Early X-Ray Crystallography for Biologics; 2.2 Modern X-ray Crystallography; 2.2.1 Construct Design and Protein Production; 2.2.2 Macromolecular Crystallization; 2.3 X-ray Data Collection; 2.3.1 Crystal Mounting; 2.3.2 Collecting a Data Set 2.3.3 Data Reduction2.4 Solving the Structure of the Crystal; 2.4.1 Molecular Replacement; 2.4.2 Heavy Atom Techniques; 2.4.3 Confirming the Validity of a Solution; 2.4.4 Building and Refining the Structure; 2.5 Understanding the Target Through Structure; 2.5.1 The Model; 2.5.2 The Protein Databank and Related Resources; 2.5.3 Information Provided by X-Ray Crystallography; 2.6 Applications of X-ray Crystallography to Biotherapeutics; 2.6.1 Antibody-Based Biotherapeutics; 2.6.2 Antibody Design; 2.6.3 Protein Receptor Interactions 2.7 Future Applications of Crystal Structures in Biotherapeutics2.7.1 Protein Engineering; 2.8 Conclusion; Acknowledgments; References; 3 Solubility and Early Assessment of Stability for Protein Therapeutics; 3.1 Introduction; 3.2 Measuring Protein Solubility; 3.2.1 Direct Measurement of Solubility: Concentration to Precipitation; 3.2.2 Indirect Assessment of Solubility: The Second Virial Coefficient (B22) and Self-Interaction Chromatography; 3.3 Assessment of Protein Stability; 3.3.1 Thermal Stability; 3.3.2 Aggregation; 3.3.3 Chemical Modifications; 3.4 Computational Predictions 3.4.1 Identifying Aggregation Promoting Regions3.4.2 Interaction Hot Spots; 3.5 Enhance the Solubility of Biotherapeutics; 3.5.1 Site-Directed Mutagenesis; 3.5.2 Pegylation; 3.5.3 Glycosylation; 3.5.4 Formulation Optimization; 3.6 Development of Rapid Methods to Identify Soluble and Stable Biotherapeutics; 3.7 Concluding Remarks; References; Section 2 First-in-Human and Up To Proof-of-Concept Clinical Trials; 4 Biophysical and Structural Characterization Needed Prior to Proof of Concept; 4.1 Introduction 4.2 Biophysical Methods for Elucidation of Protein Structure and Physiochemical Properties |
| Record Nr. | UNINA-9910822909903321 |
| Hoboken, New Jersey : , : John Wiley & Sons, , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||