| Autore |
Coppi Elisabetta
|
| Pubbl/distr/stampa |
Firenze : , : Firenze University Press, , [2008]
|
| Descrizione fisica |
1 online resource (192 pages) : illustrations; digital, PDF file(s)
|
| Disciplina |
612.39
|
| Collana |
Premio FUP. Tesi di dottorato
|
| Soggetto topico |
Purines - Receptors
|
| Formato |
Materiale a stampa  |
| Livello bibliografico |
Monografia |
| Lingua di pubblicazione |
eng
|
| Nota di contenuto |
Abbreviations -- Introduction -- 1. Historical overview -- 2. Synthesis, release and catabolism of ATP -- 2.1 Vesicular release of ATP -- 2.2 Anion channel-mediated release of ATP -- 2.3 Connexin hemichannel-mediated ATP release -- 3. Synthesis, release and catabolism of adenosine -- 3.1 Adenosine release -- 3.2 Synthesis of adenosine at extracellular level -- Purinergic receptors -- 1. P1 receptors -- 2. Pharmacology of P1 receptors -- 3. Distribution of P1 receptors -- 3.1 Central Nervous System (CNS) -- 4. P2 receptors -- 5. P2X receptors -- 6. Pharmacology of P2X receptors -- 6.1 Agonists -- 6.2 Antagonists -- 7. P2Y receptors -- 8. Pharmacology of P2Y receptors -- 8.1 Agonists -- 8.2 Antagonists -- 9. Distribution of P2 receptors -- 9.1 Neuronal localization of P2X receptors -- 9.2 Neuronal localization of P2Y receptors -- Effects of extracellular purines -- 1. Role of P1 receptors -- 1.1 P1 receptors and neurotransmission -- 1.1 P1 receptors and cerebral ischemia -- 2. Anoxic depolarization -- 3. Role of P2 receptors -- 3.1 P2 receptors and neurotransmission -- 3.2 P2 receptors and cerebral ischemia -- 3.3 P2 receptors and trophic effects -- Aim of the study -- Materials and methods -- 1. Extracellular recordings -- 1.1 Acute rat hippocampal slices preparation -- 1.2 Experimental procedure -- 1.3 Protocols used -- 1.4 OGD conditions -- 2. Patch clamp recordings -- 3. Cell cultures -- 4. Solutions -- 5. Patch pipettes fabrication -- 6. Experimental procedures -- 7. Voltage clamp protocols -- 8. Current clamp experiments -- 9. Measurement of ATP release from hMSCs -- 10. Chemicals -- 11. Statistical analysis -- RESULTS. Section 1 ROLE OF PURINES IN CA1 HIPPOCAMPAL NEUROTRANSMISSION UNDER NORMOXIC CONDITIONS AND DURING OGD. EXTRACELLULAR RECORDINGS FROM RAT HIPPOCAMPAL SLICES -- Chapter 1 Role of A3 adenosine receptors during OGD -- 1. Historical background -- 2. Results -- 2.1 Experiments were performed on a total of 219 slices taken from 133 rats -- 3. Discussion -- 3.1 Role of adenosine receptor antagonists in depression and disruption of CA1 hippocampal excitatory synaptic transmission during in vitro ischemia -- 3.2 Therapeutic implications for the protective role of adenosine A3 receptor antagonists during prolonged ischemic conditions -- 3.3 Role of adenosine receptor agonists in depression and disruption of CA1 hippocampal excitatory synaptic transmission during in vitro ischemia -- 3.5 Conclusions -- Chapter 2 Role of P2 purinergic receptors on hippocampal CA1 neurotransmission under normoxic conditions and during OGD -- 1. Historical background -- 2. Results -- 2.1 Effects of ATP on CA1 hippocampal neurotransmission -- 2.2 Effects of ATP?S on CA1 hippocampal neurotransmission -- 2.3 Effects of ATP and ATP?S on PPF in the CA1 region of rat hippocampus -- 2.4 Effects of different purinergic antagonists on OGD-evoked disruption of neurotransmission in the CA1 region of rat hippocampal slices -- 3. Discussion -- 3.1 Role of P2 receptors in hippocampal neurotransmission under normoxic conditions -- 3.2 Role of P2 receptors in hippocampal neurotransmission during OGD -- 3.3 Conclusions -- RESULTS. Section 2 -- STUDIES ON P2 RECEPTOR MODULATION OF MEMBRANE IONIC CURRENTS BY PATCH CLAMP RECORDINGS -- Chapter 3 Role of P2Y1 receptor stimulation in medium spiny neurons of rat striatal slices -- 1. Historical background -- 2. Results -- 2.1 P2Y1 receptor activation increases the outward currents elicited by a voltage ramp depolarization in striatal medium spiny neurons -- 2.2 Characterization of the P2Y1 -activated conductance in striatal medium spiny neurones -- 2.3 Single channel recordings show SK potassium channel opening following P2Y1 receptor activation -- 2.4 2MeS-ADP decreases the firing rate of medium spiny neurons recorded under the current-clamp mode in striatal slices -- 2.5 Spontaneous miniature synaptic currents are increased in amplitude during the application of 2meS-ADP -- 3. Discussion -- Chapter 4 Role of purine nucleotides in cultured hMSCs: release of ATP and activation of P2 receptors -- 1. Historical background -- 2. Results -- 2.1 Electrophysiological experiments -- 2.2 Effects of exogenous ATP in potassium-free conditions -- 2.3 Effects of ATP in hMSCs in the perforated patch-clamp configuration -- 3. Discussion -- 3.1 Conclusion -- Chapter 5 Pharmacological characterization of the recently deorphanized GPR17 receptor transfected in 1321N1 astrocytoma cells -- 1. Historical background -- 2. Results -- 2.1 Exogenously applied UDP-glucose and UDP-galactose activate outward potassium currents in 1321N1 astrocytoma cell stably transfected with GPR17 -- 2.2 GPR17 receptor, stably transfected1321N1 astrocytoma cells, is also sensitive to leukotriene-related compounds -- 3. Discussion -- Final remarks -- References.
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| Record Nr. | UNINA-9910774883603321 |
Info
Purines as transmitter molecules : electrophysiological studies on purinergic signalling in different cell systems / / Elisabetta Coppi
