Info
- Utilizzare la checkbox di selezione a fianco di ciascun documento per attivare le funzionalità di stampa, invio email, download nei formati disponibili del (i) record.
Label-free biosensors : techniques and applications / / edited by Matthew A. Cooper [[electronic resource]]
| Label-free biosensors : techniques and applications / / edited by Matthew A. Cooper [[electronic resource]] |
| Pubbl/distr/stampa | Cambridge : , : Cambridge University Press, , 2009 |
| Descrizione fisica | 1 online resource (x, 288 pages) : digital, PDF file(s) |
| Disciplina | 610.28/4 |
| Soggetto topico | Biosensors |
| ISBN |
1-107-20037-7
1-283-33018-0 9786613330185 1-139-13469-8 1-139-12964-3 1-139-13357-8 0-511-50474-8 0-511-62653-3 0-511-50688-0 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Label-free optical biosensors : an introduction / Brian T. Cunningham -- Experimental design / Robert Karlsson -- Extracting affinity constants from biosensor binding responses / Rebecca L. Rich and David G. Myszka -- Extracting kinetic rate constants from binding responses / Rebecca L. Rich and David G. Myszka -- Sensor surfaces and receptor deposition / Matthew A. Cooper -- Macromolecular interactions / Francis Markey -- Interactions with membranes and membrane receptors / Matthew A. Cooper -- Application of SPR technology to pharmaceutical relevant drug-receptor interactions / Walter Huber -- High-throughput analysis of biomolecular interactions and cellular responses with resonant waveguide grating biosensors / Ye Fang ... [et al.] -- ITC-derived binding constants : using microgram quantities of protein / Richard K. Brown ... [et al.] -- Electrical impedance technology applied to cell-based assays / Ryan P. McGuinness and Edward Verdonk. |
| Record Nr. | UNINA-9910453969803321 |
| Cambridge : , : Cambridge University Press, , 2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Label-free biosensors : techniques and applications / / edited by Matthew A. Cooper [[electronic resource]]
| Label-free biosensors : techniques and applications / / edited by Matthew A. Cooper [[electronic resource]] |
| Pubbl/distr/stampa | Cambridge : , : Cambridge University Press, , 2009 |
| Descrizione fisica | 1 online resource (x, 288 pages) : digital, PDF file(s) |
| Disciplina | 610.28/4 |
| Soggetto topico | Biosensors |
| ISBN |
1-107-20037-7
1-283-33018-0 9786613330185 1-139-13469-8 1-139-12964-3 1-139-13357-8 0-511-50474-8 0-511-62653-3 0-511-50688-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Label-free optical biosensors : an introduction / Brian T. Cunningham -- Experimental design / Robert Karlsson -- Extracting affinity constants from biosensor binding responses / Rebecca L. Rich and David G. Myszka -- Extracting kinetic rate constants from binding responses / Rebecca L. Rich and David G. Myszka -- Sensor surfaces and receptor deposition / Matthew A. Cooper -- Macromolecular interactions / Francis Markey -- Interactions with membranes and membrane receptors / Matthew A. Cooper -- Application of SPR technology to pharmaceutical relevant drug-receptor interactions / Walter Huber -- High-throughput analysis of biomolecular interactions and cellular responses with resonant waveguide grating biosensors / Ye Fang ... [et al.] -- ITC-derived binding constants : using microgram quantities of protein / Richard K. Brown ... [et al.] -- Electrical impedance technology applied to cell-based assays / Ryan P. McGuinness and Edward Verdonk. |
| Record Nr. | UNINA-9910782614103321 |
| Cambridge : , : Cambridge University Press, , 2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Label-free biosensors : techniques and applications / / edited by Matthew A. Cooper [[electronic resource]]
| Label-free biosensors : techniques and applications / / edited by Matthew A. Cooper [[electronic resource]] |
| Pubbl/distr/stampa | Cambridge : , : Cambridge University Press, , 2009 |
| Descrizione fisica | 1 online resource (x, 288 pages) : digital, PDF file(s) |
| Disciplina | 610.28/4 |
| Soggetto topico | Biosensors |
| ISBN |
1-107-20037-7
1-283-33018-0 9786613330185 1-139-13469-8 1-139-12964-3 1-139-13357-8 0-511-50474-8 0-511-62653-3 0-511-50688-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Label-free optical biosensors : an introduction / Brian T. Cunningham -- Experimental design / Robert Karlsson -- Extracting affinity constants from biosensor binding responses / Rebecca L. Rich and David G. Myszka -- Extracting kinetic rate constants from binding responses / Rebecca L. Rich and David G. Myszka -- Sensor surfaces and receptor deposition / Matthew A. Cooper -- Macromolecular interactions / Francis Markey -- Interactions with membranes and membrane receptors / Matthew A. Cooper -- Application of SPR technology to pharmaceutical relevant drug-receptor interactions / Walter Huber -- High-throughput analysis of biomolecular interactions and cellular responses with resonant waveguide grating biosensors / Ye Fang ... [et al.] -- ITC-derived binding constants : using microgram quantities of protein / Richard K. Brown ... [et al.] -- Electrical impedance technology applied to cell-based assays / Ryan P. McGuinness and Edward Verdonk. |
| Record Nr. | UNINA-9910826813903321 |
| Cambridge : , : Cambridge University Press, , 2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Label-free technologies for drug discovery [[electronic resource] /] / editors, Matthew Cooper, Lorenz Mayr
| Label-free technologies for drug discovery [[electronic resource] /] / editors, Matthew Cooper, Lorenz Mayr |
| Pubbl/distr/stampa | Chichester, West Sussex, U.K., : John Wiley & Sons, 2011 |
| Descrizione fisica | 1 online resource (350 p.) |
| Disciplina |
615.19
615/.19 |
| Altri autori (Persone) |
CooperM. A (Matthew A.)
MayrLorenz |
| Soggetto topico |
Drug development
Biosensors Pharmaceutical biotechnology |
| ISBN |
1-119-99027-0
1-283-37396-3 9786613373960 0-470-97913-5 0-470-97912-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Label-Free TechnologiesFor Drug Discovery; Contents; Preface; List of Contributors; 1 The Revolution of Real-Time, Label-Free Biosensor Applications; 1.1 Introduction; 1.2 SPR Pessimists; 1.3 Setting Up Experiments; 1.4 Data Processing and Analysis; 1.5 The Good News; References; 2 Design and Implementation of Vertically Emitting Distributed Feedback Lasers for Biological Sensing; 2.1 Introduction; 2.2 DFB Laser Biosensor Design; 2.3 Fabrication and Instrumentation; 2.4 Experimental Results; 2.4.1 Vertically Emitting DFB Laser; 2.4.2 Bulk Material Sensing; 2.4.3 Sensitivity Resolution
2.4.4 Small Molecule Binding Detection2.5 Conclusions; Acknowledgements; References; 3 SPR Screening of Chemical Microarrays for Fragment-Based Discovery; 3.1 Introduction; 3.2 Key Features of Fragment Screening; 3.3 SPR Fragment Screening; 3.4 Synthesis of Library Compounds; 3.5 Library Design and Array Content; 3.6 Chemical Microarray Production; 3.7 Surface Plasmon Resonance; 3.8 SPR Imaging; 3.9 Array Visualization and Analysis; 3.10 Follow-up; 3.11 Applications: MMP Case Study; 3.11.1 Search for New Binding Modes; 3.11.2 Selectivity Studies; 3.12 Other Target Classes; 3.13 Conclusion References4 The CellKey R System: A Label-Free Cell-Based Assay Platform for Early Drug Discovery Applications; 4.1 Introduction; 4.2 Cellular Impedance Technology; 4.3 Target Identification and Validation; 4.4 Screening and Lead Optimization; 4.5 Conclusion; References; 5 Dynamic and Label-Free Cell-Based Assays Using the xCELLigence System; 5.1 Introduction; 5.2 The xCELLigence System; 5.3 Principle of Detection; 5.4 Applications; 5.4.1 Cell Proliferation, Cytotoxicity and Time Dependent Cellular Response Profiling; 5.5 Functional Assays for G-Protein Coupled Receptors; 5.6 Conclusion References6 Selecting the Best HTS Hits to Move Forward: ITC Ligand Binding Characterization Provides Guidance; 6.1 Introduction; 6.2 Principles of Isothermal Titration Calorimetry (ITC); 6.3 Applications of ITC in Hit Validation; 6.3.1 Assay Design for Hit Confirmation and Affinity Determination; 6.3.2 Identification of Nonspecific Binders, Unstable Protein Targets and Multiple Binding Sites; 6.3.3 High Speed ITC Hit Characterization Assays; 6.4 Applications of ITC in Fragment-Based Drug Discovery; 6.4.1 Measuring Weak Affinities by ITC; 6.5 Applications of ITC in Mechanism of Action Studies 6.6 Applications of ITC in Lead Optimization6.7 ITC as an Enzyme Activity Monitor; 6.8 Conclusion; References; 7 Incorporating Transmitted Light Modalities into High-Content Analysis Assays; 7.1 Introduction; 7.2 Transmitted Light (Bright Field) Imaging; 7.3 Image Analysis of Phase Contrast Images; 7.4 Conclusion; References; 8 Nonradioactive Rubidium Efflux Assay Technology for Screening of Ion Channels; 8.1 Introduction; 8.2 Ion Channels as Drug Targets; 8.3 Ion Channel Assays and Screening; 8.4 Nonradioactive Rubidium Efflux Assay Based on Atomic Absorption Spectrometry 8.5 A Typical Assay Protocol |
| Record Nr. | UNINA-9910133454603321 |
| Chichester, West Sussex, U.K., : John Wiley & Sons, 2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Label-free technologies for drug discovery [[electronic resource] /] / editors, Matthew Cooper, Lorenz Mayr
| Label-free technologies for drug discovery [[electronic resource] /] / editors, Matthew Cooper, Lorenz Mayr |
| Pubbl/distr/stampa | Chichester, West Sussex, U.K., : John Wiley & Sons, 2011 |
| Descrizione fisica | 1 online resource (350 p.) |
| Disciplina |
615.19
615/.19 |
| Altri autori (Persone) |
CooperM. A (Matthew A.)
MayrLorenz |
| Soggetto topico |
Drug development
Biosensors Pharmaceutical biotechnology |
| ISBN |
1-119-99027-0
1-283-37396-3 9786613373960 0-470-97913-5 0-470-97912-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Label-Free TechnologiesFor Drug Discovery; Contents; Preface; List of Contributors; 1 The Revolution of Real-Time, Label-Free Biosensor Applications; 1.1 Introduction; 1.2 SPR Pessimists; 1.3 Setting Up Experiments; 1.4 Data Processing and Analysis; 1.5 The Good News; References; 2 Design and Implementation of Vertically Emitting Distributed Feedback Lasers for Biological Sensing; 2.1 Introduction; 2.2 DFB Laser Biosensor Design; 2.3 Fabrication and Instrumentation; 2.4 Experimental Results; 2.4.1 Vertically Emitting DFB Laser; 2.4.2 Bulk Material Sensing; 2.4.3 Sensitivity Resolution
2.4.4 Small Molecule Binding Detection2.5 Conclusions; Acknowledgements; References; 3 SPR Screening of Chemical Microarrays for Fragment-Based Discovery; 3.1 Introduction; 3.2 Key Features of Fragment Screening; 3.3 SPR Fragment Screening; 3.4 Synthesis of Library Compounds; 3.5 Library Design and Array Content; 3.6 Chemical Microarray Production; 3.7 Surface Plasmon Resonance; 3.8 SPR Imaging; 3.9 Array Visualization and Analysis; 3.10 Follow-up; 3.11 Applications: MMP Case Study; 3.11.1 Search for New Binding Modes; 3.11.2 Selectivity Studies; 3.12 Other Target Classes; 3.13 Conclusion References4 The CellKey R System: A Label-Free Cell-Based Assay Platform for Early Drug Discovery Applications; 4.1 Introduction; 4.2 Cellular Impedance Technology; 4.3 Target Identification and Validation; 4.4 Screening and Lead Optimization; 4.5 Conclusion; References; 5 Dynamic and Label-Free Cell-Based Assays Using the xCELLigence System; 5.1 Introduction; 5.2 The xCELLigence System; 5.3 Principle of Detection; 5.4 Applications; 5.4.1 Cell Proliferation, Cytotoxicity and Time Dependent Cellular Response Profiling; 5.5 Functional Assays for G-Protein Coupled Receptors; 5.6 Conclusion References6 Selecting the Best HTS Hits to Move Forward: ITC Ligand Binding Characterization Provides Guidance; 6.1 Introduction; 6.2 Principles of Isothermal Titration Calorimetry (ITC); 6.3 Applications of ITC in Hit Validation; 6.3.1 Assay Design for Hit Confirmation and Affinity Determination; 6.3.2 Identification of Nonspecific Binders, Unstable Protein Targets and Multiple Binding Sites; 6.3.3 High Speed ITC Hit Characterization Assays; 6.4 Applications of ITC in Fragment-Based Drug Discovery; 6.4.1 Measuring Weak Affinities by ITC; 6.5 Applications of ITC in Mechanism of Action Studies 6.6 Applications of ITC in Lead Optimization6.7 ITC as an Enzyme Activity Monitor; 6.8 Conclusion; References; 7 Incorporating Transmitted Light Modalities into High-Content Analysis Assays; 7.1 Introduction; 7.2 Transmitted Light (Bright Field) Imaging; 7.3 Image Analysis of Phase Contrast Images; 7.4 Conclusion; References; 8 Nonradioactive Rubidium Efflux Assay Technology for Screening of Ion Channels; 8.1 Introduction; 8.2 Ion Channels as Drug Targets; 8.3 Ion Channel Assays and Screening; 8.4 Nonradioactive Rubidium Efflux Assay Based on Atomic Absorption Spectrometry 8.5 A Typical Assay Protocol |
| Record Nr. | UNINA-9910830744603321 |
| Chichester, West Sussex, U.K., : John Wiley & Sons, 2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||