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In situ hybridization [[electronic resource] /] / Melody Clark
| In situ hybridization [[electronic resource] /] / Melody Clark |
| Pubbl/distr/stampa | [Saddle River, NJ?, : Wiley-VCH, 2002?] |
| Descrizione fisica | 1 online resource (182 p.) |
| Disciplina |
572.84
574.87322 |
| Altri autori (Persone) | ClarkMelody |
| Collana | Laboratory companion |
| Soggetto topico |
In situ hybridization
Genetics |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-75854-X
9786611758547 3-527-61507-5 3-527-61506-7 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
In Situ Hybridization; Contents; CHAPTER 1. Genomic In Situ Hybridization for Whole Chromosome and Genome Analysis; 1.1 Introduction; 1.2 Uses of Genomic In Situ Hybridization; 1.2.1 Genome Organization of Different Sequence Classes; 1.2.2 Species Identity, Differentiation and Relatedness; 1.2.3 Genomic Stability; 1.2.4 Spatial Organization of Chromosomes; 1.2.5 Chromosome lntrogression and Rearrangements; 1.2.6 Chromosome Behaviour and Meiosis; 1.3 The Principle of Genomic In Situ Hybridization; 1.3.1 Genomic Probing; 1.3.2 Blocking and Competitive Hybridization; 1.4 Materials and Chemicals
1.4.1 Buffers and Reagents1.4.2 Chemicals; 1.4.3 Equipment; 1.5 Genomic In Situ Hybridization: Protocol; 1.5.1 Chromosome Preparations; 1.5.2 Probe DNA; 1.5.3 Blocking DNA; 1.5.4 In Situ Hybridization Procedure; 1.6 Troubleshooting; CHAPTER 2. Fluorescence In Situ Hybridization: Applications in Gene Mapping and Clinical Diagnostics; 2.1 Introduction; 2.2 Materials and Chemicals; 2.2.1 Buffers and Reagents; 2.2.2 Chemicals; 2.2.3 Equipment; 2.3 FISH Using Single Copy Cosmid Probes; 2.3.1 Preparation of Metaphase Chromosomes; 2.3.2 Preparation of Metaphase Chromosome Slides for Hybridization 2.3.3 Preparation and Labelling of Probes2.3.4 Hybridization of Cosmid Probes; 2.4 Multicolor FISH Using Cosmid Probes; 2.4.1 Labelling of Probes and Hybridization Reactions; 2.5 lnterphase FISH Analysis; 2.5.1 Preparation of lnterphase Nuclear Slides from Short Term Cultures; 2.5.2 Direct Preparation of lnterphase Spreads; 2.5.3 FISH; 2.6 FISH Analysis Using YAC Clones; 2.6.1 Preparation of Metaphase Chromosomes; 2.6.2 Isolation of YAC DNA; 2.6.3 Biotin Labelling of YACs; 2.6.4 FISH; 2.6.5 Microscopy; 2.7 Direct Visual In Situ Hybridization (DIRVISH); 2.7.1 Preparation of Stretched DNA 2.7.2 FISH Hybridization2.7.3 Detection and Analysis; 2.7.4 Microscopy; 2.8 Troubleshooting; CHAPTER 3. Detection of Nucleic Acids (DNA and RNA) In Situ by Single and Cyclic Primed In Situ labelling (PRINS): Two Alternatives to Traditional In Situ Hybridization Methods; 3.1 Introduction; 3.2 Materials and Chemicals; 3.2.1 Buffers and Reagents; 3.2.2 Chemicals; 3.2.3 Equipment; 3.3 DNA-PRINS with Oligonucleotide Probes; 3.4 PRINS with Ddel Digested Cloned Probes; 3.4.1 Generation of Primers from Cloned Alpha Satellite DNA; 3.4.2 PRlNS Reaction; 3.5 Multicolour-PRINS; 3.6 PRINS-painting 3.7 PRINS-PCR and Repeated-PRINS of DNA3.7.1 Chromosome Spreads; 3.7.2 Pretreatment of Chromosome Spreads; 3.7.3 Labelling Reaction; 3.8 PRINS-PCR of mRNA; 3.9 Visualization of Hapten-Labelled Nucleotides; 3.9.1 Standard Slides; 3.9.1.1 Biotin; 3.9.1.2 Digoxigenin; 3.9.2 Micro-slides; 3.9.2.1 Detection of Labelled DNA; 3.9.2.2 Detection of Labelled RNA; 3.9.2.3 Alkaline Phosphatase Visualization; 3.9.2.4 Fluorescent Visualization; 3.10 Troubleshooting CHAPTER 4. Fluorescence lmmunophenotyping and lnterphase Cytogenetics as a Tool for Investigation of Neoplasms (FICTION): Combined In Situ Hybridization and Fluorescence lmmunophenotyping |
| Record Nr. | UNINA-9910143989303321 |
| [Saddle River, NJ?, : Wiley-VCH, 2002?] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
In situ hybridization [[electronic resource] /] / Melody Clark
| In situ hybridization [[electronic resource] /] / Melody Clark |
| Pubbl/distr/stampa | [Saddle River, NJ?, : Wiley-VCH, 2002?] |
| Descrizione fisica | 1 online resource (182 p.) |
| Disciplina |
572.84
574.87322 |
| Altri autori (Persone) | ClarkMelody |
| Collana | Laboratory companion |
| Soggetto topico |
In situ hybridization
Genetics |
| ISBN |
1-281-75854-X
9786611758547 3-527-61507-5 3-527-61506-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
In Situ Hybridization; Contents; CHAPTER 1. Genomic In Situ Hybridization for Whole Chromosome and Genome Analysis; 1.1 Introduction; 1.2 Uses of Genomic In Situ Hybridization; 1.2.1 Genome Organization of Different Sequence Classes; 1.2.2 Species Identity, Differentiation and Relatedness; 1.2.3 Genomic Stability; 1.2.4 Spatial Organization of Chromosomes; 1.2.5 Chromosome lntrogression and Rearrangements; 1.2.6 Chromosome Behaviour and Meiosis; 1.3 The Principle of Genomic In Situ Hybridization; 1.3.1 Genomic Probing; 1.3.2 Blocking and Competitive Hybridization; 1.4 Materials and Chemicals
1.4.1 Buffers and Reagents1.4.2 Chemicals; 1.4.3 Equipment; 1.5 Genomic In Situ Hybridization: Protocol; 1.5.1 Chromosome Preparations; 1.5.2 Probe DNA; 1.5.3 Blocking DNA; 1.5.4 In Situ Hybridization Procedure; 1.6 Troubleshooting; CHAPTER 2. Fluorescence In Situ Hybridization: Applications in Gene Mapping and Clinical Diagnostics; 2.1 Introduction; 2.2 Materials and Chemicals; 2.2.1 Buffers and Reagents; 2.2.2 Chemicals; 2.2.3 Equipment; 2.3 FISH Using Single Copy Cosmid Probes; 2.3.1 Preparation of Metaphase Chromosomes; 2.3.2 Preparation of Metaphase Chromosome Slides for Hybridization 2.3.3 Preparation and Labelling of Probes2.3.4 Hybridization of Cosmid Probes; 2.4 Multicolor FISH Using Cosmid Probes; 2.4.1 Labelling of Probes and Hybridization Reactions; 2.5 lnterphase FISH Analysis; 2.5.1 Preparation of lnterphase Nuclear Slides from Short Term Cultures; 2.5.2 Direct Preparation of lnterphase Spreads; 2.5.3 FISH; 2.6 FISH Analysis Using YAC Clones; 2.6.1 Preparation of Metaphase Chromosomes; 2.6.2 Isolation of YAC DNA; 2.6.3 Biotin Labelling of YACs; 2.6.4 FISH; 2.6.5 Microscopy; 2.7 Direct Visual In Situ Hybridization (DIRVISH); 2.7.1 Preparation of Stretched DNA 2.7.2 FISH Hybridization2.7.3 Detection and Analysis; 2.7.4 Microscopy; 2.8 Troubleshooting; CHAPTER 3. Detection of Nucleic Acids (DNA and RNA) In Situ by Single and Cyclic Primed In Situ labelling (PRINS): Two Alternatives to Traditional In Situ Hybridization Methods; 3.1 Introduction; 3.2 Materials and Chemicals; 3.2.1 Buffers and Reagents; 3.2.2 Chemicals; 3.2.3 Equipment; 3.3 DNA-PRINS with Oligonucleotide Probes; 3.4 PRINS with Ddel Digested Cloned Probes; 3.4.1 Generation of Primers from Cloned Alpha Satellite DNA; 3.4.2 PRlNS Reaction; 3.5 Multicolour-PRINS; 3.6 PRINS-painting 3.7 PRINS-PCR and Repeated-PRINS of DNA3.7.1 Chromosome Spreads; 3.7.2 Pretreatment of Chromosome Spreads; 3.7.3 Labelling Reaction; 3.8 PRINS-PCR of mRNA; 3.9 Visualization of Hapten-Labelled Nucleotides; 3.9.1 Standard Slides; 3.9.1.1 Biotin; 3.9.1.2 Digoxigenin; 3.9.2 Micro-slides; 3.9.2.1 Detection of Labelled DNA; 3.9.2.2 Detection of Labelled RNA; 3.9.2.3 Alkaline Phosphatase Visualization; 3.9.2.4 Fluorescent Visualization; 3.10 Troubleshooting CHAPTER 4. Fluorescence lmmunophenotyping and lnterphase Cytogenetics as a Tool for Investigation of Neoplasms (FICTION): Combined In Situ Hybridization and Fluorescence lmmunophenotyping |
| Record Nr. | UNINA-9910830987403321 |
| [Saddle River, NJ?, : Wiley-VCH, 2002?] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
In situ hybridization [[electronic resource] /] / Melody Clark
| In situ hybridization [[electronic resource] /] / Melody Clark |
| Pubbl/distr/stampa | [Saddle River, NJ?, : Wiley-VCH, 2002?] |
| Descrizione fisica | 1 online resource (182 p.) |
| Disciplina |
572.84
574.87322 |
| Altri autori (Persone) | ClarkMelody |
| Collana | Laboratory companion |
| Soggetto topico |
In situ hybridization
Genetics |
| ISBN |
1-281-75854-X
9786611758547 3-527-61507-5 3-527-61506-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
In Situ Hybridization; Contents; CHAPTER 1. Genomic In Situ Hybridization for Whole Chromosome and Genome Analysis; 1.1 Introduction; 1.2 Uses of Genomic In Situ Hybridization; 1.2.1 Genome Organization of Different Sequence Classes; 1.2.2 Species Identity, Differentiation and Relatedness; 1.2.3 Genomic Stability; 1.2.4 Spatial Organization of Chromosomes; 1.2.5 Chromosome lntrogression and Rearrangements; 1.2.6 Chromosome Behaviour and Meiosis; 1.3 The Principle of Genomic In Situ Hybridization; 1.3.1 Genomic Probing; 1.3.2 Blocking and Competitive Hybridization; 1.4 Materials and Chemicals
1.4.1 Buffers and Reagents1.4.2 Chemicals; 1.4.3 Equipment; 1.5 Genomic In Situ Hybridization: Protocol; 1.5.1 Chromosome Preparations; 1.5.2 Probe DNA; 1.5.3 Blocking DNA; 1.5.4 In Situ Hybridization Procedure; 1.6 Troubleshooting; CHAPTER 2. Fluorescence In Situ Hybridization: Applications in Gene Mapping and Clinical Diagnostics; 2.1 Introduction; 2.2 Materials and Chemicals; 2.2.1 Buffers and Reagents; 2.2.2 Chemicals; 2.2.3 Equipment; 2.3 FISH Using Single Copy Cosmid Probes; 2.3.1 Preparation of Metaphase Chromosomes; 2.3.2 Preparation of Metaphase Chromosome Slides for Hybridization 2.3.3 Preparation and Labelling of Probes2.3.4 Hybridization of Cosmid Probes; 2.4 Multicolor FISH Using Cosmid Probes; 2.4.1 Labelling of Probes and Hybridization Reactions; 2.5 lnterphase FISH Analysis; 2.5.1 Preparation of lnterphase Nuclear Slides from Short Term Cultures; 2.5.2 Direct Preparation of lnterphase Spreads; 2.5.3 FISH; 2.6 FISH Analysis Using YAC Clones; 2.6.1 Preparation of Metaphase Chromosomes; 2.6.2 Isolation of YAC DNA; 2.6.3 Biotin Labelling of YACs; 2.6.4 FISH; 2.6.5 Microscopy; 2.7 Direct Visual In Situ Hybridization (DIRVISH); 2.7.1 Preparation of Stretched DNA 2.7.2 FISH Hybridization2.7.3 Detection and Analysis; 2.7.4 Microscopy; 2.8 Troubleshooting; CHAPTER 3. Detection of Nucleic Acids (DNA and RNA) In Situ by Single and Cyclic Primed In Situ labelling (PRINS): Two Alternatives to Traditional In Situ Hybridization Methods; 3.1 Introduction; 3.2 Materials and Chemicals; 3.2.1 Buffers and Reagents; 3.2.2 Chemicals; 3.2.3 Equipment; 3.3 DNA-PRINS with Oligonucleotide Probes; 3.4 PRINS with Ddel Digested Cloned Probes; 3.4.1 Generation of Primers from Cloned Alpha Satellite DNA; 3.4.2 PRlNS Reaction; 3.5 Multicolour-PRINS; 3.6 PRINS-painting 3.7 PRINS-PCR and Repeated-PRINS of DNA3.7.1 Chromosome Spreads; 3.7.2 Pretreatment of Chromosome Spreads; 3.7.3 Labelling Reaction; 3.8 PRINS-PCR of mRNA; 3.9 Visualization of Hapten-Labelled Nucleotides; 3.9.1 Standard Slides; 3.9.1.1 Biotin; 3.9.1.2 Digoxigenin; 3.9.2 Micro-slides; 3.9.2.1 Detection of Labelled DNA; 3.9.2.2 Detection of Labelled RNA; 3.9.2.3 Alkaline Phosphatase Visualization; 3.9.2.4 Fluorescent Visualization; 3.10 Troubleshooting CHAPTER 4. Fluorescence lmmunophenotyping and lnterphase Cytogenetics as a Tool for Investigation of Neoplasms (FICTION): Combined In Situ Hybridization and Fluorescence lmmunophenotyping |
| Record Nr. | UNINA-9910841209003321 |
| [Saddle River, NJ?, : Wiley-VCH, 2002?] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||