Info
- Utilizzare la checkbox di selezione a fianco di ciascun documento per attivare le funzionalità di stampa, invio email, download nei formati disponibili del (i) record.
Stem Cell Bioprocessing and Manufacturing
| Stem Cell Bioprocessing and Manufacturing |
| Autore | Cabral Joaquim M. S |
| Pubbl/distr/stampa | Basel, Switzerland, : MDPI - Multidisciplinary Digital Publishing Institute, 2020 |
| Descrizione fisica | 1 online resource (170 p.) |
| Soggetto topico | Medicine |
| Soggetto non controllato |
adipose stem cells
adipose-derived stem cell advanced therapy medicinal products allogeneic cell therapy arthroscopy arthrotomy autologous therapy axonal regeneration bio-process bioprocess bioreactor blood platelets cell aggregate cell culture techniques cell growth cell seeding cell therapies computational fluid dynamics differentiation disease modeling drug screening electrospinning embryonic stem cells expansion fibrin nerve conduits growth factors hepatic cell lineages hepatocyte differentiation high voltage hMSCs homogeneous hydrodynamic environment human adipose stem cells (hASCs) human embryonic stem cell human pluripotent stem cells hydrogels induced pluripotent stem cell infrapatellar fat pad kinetic growth modeling knee lactate live-cell electrospinning liver organoids mesenchymal stromal cells microcarrier model predictive control n/a neural regeneration neurotrophic factors non-parenchymal liver cells olfactory ensheathing cells peripheral nerve injuries progenitor cells scalable manufacturing scale up Schwann cells segregated and unstructured growth model serum- and xeno-free conditions shear stress single-use bioreactor spinal cord injury stem cell stem cells delivery stromal vascular fraction tissue engineering turbulent energy dissipation rates U-shaped vessel UrSuppe stem cell culture medium Vertical-Wheel viability |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910557788003321 |
Cabral Joaquim M. S
|
||
| Basel, Switzerland, : MDPI - Multidisciplinary Digital Publishing Institute, 2020 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Stem Cell Manufacturing [[electronic resource]]
| Stem Cell Manufacturing [[electronic resource]] |
| Autore | Cabral Joaquim M. S |
| Pubbl/distr/stampa | Oxford, : Elsevier Science, 2015 |
| Descrizione fisica | 1 online resource (342 p.) |
| Disciplina | 616.02774 |
| Altri autori (Persone) |
da SilvaClaudia Lobato
ChaseLucas G DiogoMaria Margarida |
| Soggetto topico | Stem cells |
| ISBN |
0-444-63266-2
0-444-63265-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; STEM CELL MANUFACTURING; STEM CELL MANUFACTURING; Copyright; CONTENTS; LIST OF CONTRIBUTORS; INTRODUCTION; 1 - Genetic Engineering in Stem Cell Biomanufacturing ; 1.1 INTRODUCTION; 1.2 GENETIC MANIPULATION APPROACHES IN HUMAN PLURIPOTENT STEM CELLS; 1.2.1 Transgenic Approaches; 1.2.2 Knock-In and Knock-Out Approaches; 1.2.2.1 Zinc-Finger Nucleases; 1.2.2.2 Transcription Activator-Like Effector Nucleases; 1.2.2.3 Clustered Regularly Interspaced Short Palindromic Repeat/Cas9; 1.2.3 Bacterial Artificial Chromosomes; 1.3 APPLICATIONS
1.3.1 Genetic Labeling for Cell Identification and Cell Tracking1.3.2 Gene Alteration for Directed Differentiation; 1.3.3 Gene Disruption for Functional Investigation; 1.3.4 Gene Correction for Function Restoration; 1.4 DELIVERY METHODS; 1.4.1 Transfection; 1.4.2 Nucleofection; 1.4.3 Viral Transduction; 1.5 CONCLUSIONS; ACKNOWLEDGMENTS; REFERENCES; 2 - Biomechanics in Stem Cell Manufacturing; 2.1 INTRODUCTION; 2.2 CELLULAR BIOMECHANICS; 2.2.1 Biomechanical Cues; 2.2.2 Shear Forces and Differentiated Cells; 2.2.3 Shear Forces and Pluripotent Stem Cells 2.3 SCALE UP TOWARD PRODUCTION-LEVEL BIOREACTORS2.4 BIOMANUFACTURING CELLS FOR THERAPIES; 2.4.1 Pluripotent Stem Cells; 2.4.2 Cardiomyocytes; 2.4.3 Endothelial Cells; 2.5 CONCLUSION; REFERENCES; 3 - Bioreactor Engineering Fundamentals for Stem Cell Manufacturing ; 3.1 INTRODUCTION; 3.2 STIRRED BIOREACTOR BASICS; 3.3 SPECIAL FEATURES OF STIRRED BIOREACTORS FOR HMSC CULTURE ON MICROCARRIERS; 3.3.1 Introduction; 3.3.2 Preparing the Bioreactor for Culture; 3.3.3 Medium and Medium Exchange; 3.3.4 Microcarrier Selection; 3.3.5 Cell and Microcarrier Concentrations; 3.3.6 Attachment Protocol 3.3.7 Use of Coatings to Enhance Attachment3.3.8 The Minimum Speed for Suspension, NJS and Associated Mean Specific Energy Dissipation Rate, εT;̄ 3.3.8.1 General Aspects; 3.3.8.2 NJS Considerations in hMSC Culture; 3.3.9 Oxygen Demand, Mass Transfer, and Optimum Dissolved Oxygen; 3.3.9.1 General Considerations; 3.3.9.2 Application to hMSC Culture; 3.3.10 Fluid Dynamically Generated Stresses and Cell Proliferation; 3.3.10.1 General Considerations; 3.3.10.2 Application to hMSC Culture; 3.3.11 Fluid Dynamically Generated Stresses and Their Application to Cell Harvesting; 3.4 FUTURE ISSUES 3.4.1 Increasing Cell Density3.4.2 Oxygen Demand and Mass Transfer at Higher Cell Density-Sparging and Higher Agitator Speeds; 3.4.3 Carbon Dioxide, Osmolality, and pH; 3.4.4 Human-Induced and Embryonic Pluripotent Stem Cells; 3.5 CONCLUSIONS; NOMENCLATURE; REFERENCES; 4 - Microcarrier Culture Systems for Stem Cell Manufacturing ; 4.1 OVERVIEW; 4.1.1 Historical Perspective; 4.2 MICROCARRIER TECHNOLOGY; 4.2.1 Types of Microcarriers; 4.2.2 Properties of Microcarriers Required for Cell Culturing; 4.2.3 Advantages of Using Microcarrier Culture Systems for Cell Manufacturing 4.3 SCALABLE CULTURE SYSTEMS FOR ADHERENT STEM CELLS |
| Record Nr. | UNINA-9910798524903321 |
|
Cabral Joaquim M. S
|
||
| Oxford, : Elsevier Science, 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Stem Cell Manufacturing
| Stem Cell Manufacturing |
| Autore | Cabral Joaquim M. S |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Oxford, : Elsevier Science, 2015 |
| Descrizione fisica | 1 online resource (342 p.) |
| Disciplina | 616.02774 |
| Altri autori (Persone) |
da SilvaClaudia Lobato
ChaseLucas G DiogoMaria Margarida |
| Soggetto topico | Stem cells |
| ISBN |
9780444632661
0444632662 9780444632654 0444632654 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; STEM CELL MANUFACTURING; STEM CELL MANUFACTURING; Copyright; CONTENTS; LIST OF CONTRIBUTORS; INTRODUCTION; 1 - Genetic Engineering in Stem Cell Biomanufacturing ; 1.1 INTRODUCTION; 1.2 GENETIC MANIPULATION APPROACHES IN HUMAN PLURIPOTENT STEM CELLS; 1.2.1 Transgenic Approaches; 1.2.2 Knock-In and Knock-Out Approaches; 1.2.2.1 Zinc-Finger Nucleases; 1.2.2.2 Transcription Activator-Like Effector Nucleases; 1.2.2.3 Clustered Regularly Interspaced Short Palindromic Repeat/Cas9; 1.2.3 Bacterial Artificial Chromosomes; 1.3 APPLICATIONS
1.3.1 Genetic Labeling for Cell Identification and Cell Tracking1.3.2 Gene Alteration for Directed Differentiation; 1.3.3 Gene Disruption for Functional Investigation; 1.3.4 Gene Correction for Function Restoration; 1.4 DELIVERY METHODS; 1.4.1 Transfection; 1.4.2 Nucleofection; 1.4.3 Viral Transduction; 1.5 CONCLUSIONS; ACKNOWLEDGMENTS; REFERENCES; 2 - Biomechanics in Stem Cell Manufacturing; 2.1 INTRODUCTION; 2.2 CELLULAR BIOMECHANICS; 2.2.1 Biomechanical Cues; 2.2.2 Shear Forces and Differentiated Cells; 2.2.3 Shear Forces and Pluripotent Stem Cells 2.3 SCALE UP TOWARD PRODUCTION-LEVEL BIOREACTORS2.4 BIOMANUFACTURING CELLS FOR THERAPIES; 2.4.1 Pluripotent Stem Cells; 2.4.2 Cardiomyocytes; 2.4.3 Endothelial Cells; 2.5 CONCLUSION; REFERENCES; 3 - Bioreactor Engineering Fundamentals for Stem Cell Manufacturing ; 3.1 INTRODUCTION; 3.2 STIRRED BIOREACTOR BASICS; 3.3 SPECIAL FEATURES OF STIRRED BIOREACTORS FOR HMSC CULTURE ON MICROCARRIERS; 3.3.1 Introduction; 3.3.2 Preparing the Bioreactor for Culture; 3.3.3 Medium and Medium Exchange; 3.3.4 Microcarrier Selection; 3.3.5 Cell and Microcarrier Concentrations; 3.3.6 Attachment Protocol 3.3.7 Use of Coatings to Enhance Attachment3.3.8 The Minimum Speed for Suspension, NJS and Associated Mean Specific Energy Dissipation Rate, εT;̄ 3.3.8.1 General Aspects; 3.3.8.2 NJS Considerations in hMSC Culture; 3.3.9 Oxygen Demand, Mass Transfer, and Optimum Dissolved Oxygen; 3.3.9.1 General Considerations; 3.3.9.2 Application to hMSC Culture; 3.3.10 Fluid Dynamically Generated Stresses and Cell Proliferation; 3.3.10.1 General Considerations; 3.3.10.2 Application to hMSC Culture; 3.3.11 Fluid Dynamically Generated Stresses and Their Application to Cell Harvesting; 3.4 FUTURE ISSUES 3.4.1 Increasing Cell Density3.4.2 Oxygen Demand and Mass Transfer at Higher Cell Density-Sparging and Higher Agitator Speeds; 3.4.3 Carbon Dioxide, Osmolality, and pH; 3.4.4 Human-Induced and Embryonic Pluripotent Stem Cells; 3.5 CONCLUSIONS; NOMENCLATURE; REFERENCES; 4 - Microcarrier Culture Systems for Stem Cell Manufacturing ; 4.1 OVERVIEW; 4.1.1 Historical Perspective; 4.2 MICROCARRIER TECHNOLOGY; 4.2.1 Types of Microcarriers; 4.2.2 Properties of Microcarriers Required for Cell Culturing; 4.2.3 Advantages of Using Microcarrier Culture Systems for Cell Manufacturing 4.3 SCALABLE CULTURE SYSTEMS FOR ADHERENT STEM CELLS |
| Record Nr. | UNINA-9910973676403321 |
|
Cabral Joaquim M. S
|
||
| Oxford, : Elsevier Science, 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||