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Biophysical Chemistry of Proteins : An Introduction to Laboratory Methods / / by Engelbert Buxbaum
| Biophysical Chemistry of Proteins : An Introduction to Laboratory Methods / / by Engelbert Buxbaum |
| Autore | Buxbaum Engelbert |
| Edizione | [2nd ed. 2025.] |
| Pubbl/distr/stampa | New York, NY : , : Springer US : , : Imprint : Springer, , 2025 |
| Descrizione fisica | 1 online resource (911 pages) |
| Disciplina | 572.6 |
| Soggetto topico |
Proteins
Proteins - Synthesis Biochemistry Chemistry Protein Biochemistry Protein Synthesis and Translation |
| ISBN | 1-0716-4124-7 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Analytical techniques -- Microscopy -- Single molecule techniques -- Preparation of cells and tissues for microscopy -- Principles of optical spectroscopy -- Photometry -- Fluorimetry -- Chemiluminescence -- Electrophoresis -- Immunological methods -- Isotope techniques -- Purification of proteins -- Homogenisation and fractionisation of cells and tissues -- Isolation of organelles -- Precipitation methods -- Chromatography -- Membrane proteins -- Determination of protein concentration -- Cell culture -- Protein modification and inactivation -- General technical remarks -- Amine-reactive reagents -- Thiol- and disulphide reactive reagents -- Reagents for other groups -- Cross-linkers -- Detection methods -- Spontaneous reactions in proteins -- Protein size and shape -- Centrifugation -- Osmotic pressure -- Diffusion -- Viscosity -- Non-resonant interactions with electromagnetic waves -- Protein structure -- Protein sequencing -- Synthesis of peptides -- Protein secondary structure -- Structure of protein-ligand complexes -- 3D-structures -- Folding and unfolding of proteins -- Enzyme kinetics -- Steady-state kinetics -- Leaving the steady state: Analysis of progress curves -- Reaction velocities -- Isotope effects -- Isotope exchange -- Protein-ligand interactions -- General conditions for interpretable results -- Binding equations -- Methods to measure binding equilibria -- Temperature effects on binding equilibrium and reaction rate -- Industrial enzymology -- Industrial enzyme use -- Immobilised enzymes -- Special statistics -- Quality control -- Testing whether or not a model fits the data -- Appendix -- List of symbols -- Greek alphabet -- Properties of electrophoretic buffers -- Bond properties -- Acronyms. |
| Record Nr. | UNINA-9911015636003321 |
Buxbaum Engelbert
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| New York, NY : , : Springer US : , : Imprint : Springer, , 2025 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Fundamentals of Protein Structure and Function / / by Engelbert Buxbaum
| Fundamentals of Protein Structure and Function / / by Engelbert Buxbaum |
| Autore | Buxbaum Engelbert |
| Edizione | [2nd ed. 2015.] |
| Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Springer, , 2015 |
| Descrizione fisica | 1 online resource (XVI, 521 p. 246 illus., 223 illus. in color.) |
| Disciplina | 572.633 |
| Soggetto topico |
Biochemistry
Cytology Chemistry Medicine Biophysics Biochemistry, general Cell Biology Chemistry/Food Science, general Biomedicine, general Biological and Medical Physics, Biophysics |
| ISBN | 3-319-19920-X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Part I: Proteins -- Amino Acids -- Protein Structure -- Proteins in the Lab -- Part II: Enzymes -- Enzymes are Biocatalysts -- Enzyme Kinetics and Mechanism -- Inhibition and Inactivation of Enzymes -- Hæmyoglobin and Myoglobin: Cooperativity -- Enzyme Kinetics: Special Cases -- The Flow of Metabolites through Metabolic Pathways -- Part III: Special Proteins -- Protein Folding Diseases -- Immunoproteins -- Cell Skeleton -- Motor Proteins and Movement -- Cell-cell interactions -- Aiding in Folding: Molecular Chaperones and Chaperonins -- Part IV: Membrane Transport -- Protein Transport across Membranes -- Vesicular Transport in Eukaryotic Cells -- Transport of Solutes across Membranes -- Appendices. |
| Record Nr. | UNINA-9910298457803321 |
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Buxbaum Engelbert
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| Cham : , : Springer International Publishing : , : Imprint : Springer, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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