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Lipid modification by enzymes and engineered microbes / / edited by Uwe T. Bornscheuer
| Lipid modification by enzymes and engineered microbes / / edited by Uwe T. Bornscheuer |
| Pubbl/distr/stampa | London : , : Academic Press, an imprint of Elsevier, , [2018] |
| Descrizione fisica | 1 online resource (xii, 382 pages) : illustrations |
| Disciplina | 612.01577 |
| Soggetto topico | Lipids |
| ISBN |
0-12-813168-3
0-12-813167-5 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910583082903321 |
| London : , : Academic Press, an imprint of Elsevier, , [2018] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Protein engineering handbook [[electronic resource]] / edited by Stefan Lutz and Uwe Theo Bornscheuer
| Protein engineering handbook [[electronic resource]] / edited by Stefan Lutz and Uwe Theo Bornscheuer |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Weinheim, [Germany] : , : Wiley-VCH Verlag Gmbh & Co. KGaA, , 2009 |
| Descrizione fisica | 1 online resource (2 volumes, 1021 p.) |
| Disciplina |
615.19
660.63 |
| Soggetto topico | Protein engineering |
| ISBN |
978-3-527-63402-6
3-527-66701-6 1-283-64407-X 3-527-66702-4 3-527-63402-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Protein Engineering Handbook; Contents; Preface; List of Contributors; Volume 1; 1 Guidelines for the Functional Analysis of Engineered and Mutant Enzymes; 1.1 Introduction; 1.2 Steady-State Kinetics; 1.3 Enzyme Assays and the Acquisition of Initial Velocity Data; 1.3.1 Biological Sample Appropriate for Assay; 1.3.2 Enzymatic Assays; 1.3.3 Analysis of Initial Rate Data; 1.3.4 Determination of Functional Catalytic Site Concentrations; 1.4 Steady-State Kinetic Parameters and Their Interpretation; 1.4.1 pH-Dependence of Steady-State Kinetic Parameters; 1.4.2 Analysis of Two-Substrate Enzymes
1.5 Concluding RemarksReferences; 2 Engineering Enantioselectivity in Enzyme-Catalyzed Reactions; 2.1 Introduction; 2.2 Molecular Basis for Enantioselectivity; 2.2.1 Enzymes Stabilize Transition States for Fast-Reacting Enantiomers Better than Slow-Reacting Enantiomers; 2.2.2 The Slow-Reacting Enantiomer Fits by Exchanging Two Substituents; 2.2.3 The Slow Enantiomer Fits by an Umbrella-Like Inversion; 2.3 Qualitative Predictions of Enantioselectivity; 2.3.1 Comparing Substrate Structures Leads to Empirical Rules and Box Models; 2.3.2 Computer Modeling Based on X-Ray Structures of Enzymes 2.3.3 What Is Missing from Current Computer Modeling?2.4 Protein Engineering to Increase or Reverse Enantioselectivity; 2.4.1 Mutations Closer to the Active Site Increase Enantioselectivity More Effectively than Mutations Far from the Active Site; 2.4.2 Reversing Enantioselectivity by Exchanging Locations of Binding Sites or a Catalytic Group; 2.5 Concluding Remarks; References; 3 Mechanism and Catalytic Promiscuity: Emerging Mechanistic Principles for Identifi cation and Manipulation of Catalytically Promiscuous Enzymes; 3.1 Introduction; 3.2 Calculation of Rate Accelerations 3.3 Catalytic Features and Their Propensity for Promiscuity3.3.1 Metal Ions; 3.3.2 Recognition of Transition State Charges: Analysis of the Nature of the Transition State; 3.3.3 Catalytic Dyads and Triads; 3.3.4 General Acid/Base Catalysts in Promiscuous Functional Motifs in Catalytic Superfamilies; 3.4 Steric Effects and Structural Constriction in the Active Site: Product Promiscuity; 3.5 Medium Effects in Enzyme Active Sites; 3.6 Conclusions; References; 4 Φ-Value Analysis of Protein Folding Transition States; 4.1 Introduction; 4.2 Theoretical Principles of Protein Engineering 4.2.1 Overview4.2.2 Basic Concepts; 4.2.3 Theory of Φ-Value Analysis; 4.2.4 Relationship between Φ and Leffler α; 4.2.5 Linear Free-Energy Relationships and Denaturant Concentration; 4.3 Guidelines for the Determination of Accurate Φ-Values; 4.3.1 Buffer Preparation and Selection; 4.3.2 Optimization of Experimental Conditions; 4.3.3 Equilibrium Denaturation Experiments; 4.3.3.1 Practical Considerations; 4.3.3.2 Curve-Fitting; 4.3.4 Kinetic Measurements; 4.3.4.1 Practical Considerations; 4.3.4.2 Curve Fitting; 4.3.4.3 Error Analysis for Chevron Plots; 4.4 Conclusions; Acknowledgments References |
| Record Nr. | UNINA-9910130882603321 |
| Weinheim, [Germany] : , : Wiley-VCH Verlag Gmbh & Co. KGaA, , 2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||