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Modern techniques for pathogen detection / / edited by Jürgen Popp and Michael Bauer
| Modern techniques for pathogen detection / / edited by Jürgen Popp and Michael Bauer |
| Pubbl/distr/stampa | Weinheim, Germany : , : Wiley Blackwell, , 2015 |
| Descrizione fisica | 1 online resource (353 p.) |
| Disciplina | 616.9 |
| Soggetto topico | Pathogenic microorganisms - Detection |
| ISBN |
3-527-68798-X
3-527-68797-1 3-527-68799-8 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Contents; Preface; List of Contributors; Chapter 1 Unmet Medical Needs in Life-Threatening Infections - Caring for the Critically Ill; 1.1 Life Threatening Infections and Sepsis - Defining the Problem; 1.2 Sepsis as a ""Hidden Healthcare Disaster""; 1.3 Microorganisms and Types of Infection Triggering Sepsis; 1.4 Emerging Problems Related to Resistance in Bacterial Infections; 1.5 The Role of Fungi and Viruses; 1.6 The Need for New Approaches in Diagnostics of Life-Threatening Infection and Sepsis
1.7 Rapid and Sensitive Culture-Independent Strategies to Identify Blood Stream Infection1.8 Beyond Infection - Profiling the Immune Response of the Septic Host; 1.9 Host Factors Contributing to Pathogenesis of Sepsis; References; Chapter 2 Identification Methods - An Overview; 2.1 Taxonomy of Pathogenic Organisms; 2.2 Microscopic Methods; 2.2.1 Stains; 2.2.2 Autofluorescence; 2.2.3 Immunofluorescence; 2.2.4 In Situ Hybridization; 2.2.5 Dark-Field Microscopy; 2.3 Culture-Based Methods; 2.3.1 Blood Culture; 2.3.2 Automated Differentiation Systems; 2.3.3 API System; 2.3.4 Selective Agar 2.3.5 Susceptibility Testing2.3.6 Other Methods; 2.4 Nucleic Acid-Based Techniques; 2.4.1 PCR; 2.4.1.1 Real-Time PCR; 2.4.1.2 Multiplex-PCR; 2.4.1.3 RAPD and rep-PCR; 2.4.1.4 Microarrays; 2.4.1.5 RFLP; 2.4.2 Sequencing; 2.4.2.1 Ribosomal RNA Genes; 2.4.2.2 MLST; 2.4.2.3 NG Sequencing; 2.5 Serology; 2.5.1 Antigen-Antibody-Based Methods; 2.5.1.1 Agglutination; 2.5.1.2 Immunodiffusion; 2.5.1.3 Quantitative Immunoassays; 2.5.2 Automated Immunoassays; 2.5.3 Applications of Serological Test; 2.5.3.1 Types of Human Antibodies and What They Indicate? 2.5.3.2 Validity of Antibodies against Opportunistic Species2.5.3.3 Specificity of Fungal Antigens; 2.5.3.4 Specific Urine Antigen Tests; 2.5.4 Biomarkers; 2.6 Conclusions and Perspectives; References; Chapter 3 Nucleic Acid Amplification Techniques; 3.1 Introduction; 3.2 The Basic PCR Protocol; 3.3 Primer Design; 3.3.1 Specific versus Broad-Range Primers and the Fate of False Targeting; 3.4 Modified End-Point PCR Protocols; 3.5 Non-PCR NAT: Isothermal Amplification Protocols; 3.6 Quantitative PCR; 3.6.1 Quantification in qPCR; 3.6.2 Melting Curve Analysis (MCA) 3.7 Controls, Probing, and General Aspects of Result Interpretation3.7.1 Strategies in Amplicon Verification; 3.7.1.1 (DNA) Microarray; 3.7.1.2 Flow Cytometry; 3.7.1.3 Mass Spectrometry; 3.7.2 PCR Inhibitors; 3.8 Preanalytics; 3.8.1 Sample Volume; 3.8.2 Cell Lysis; 3.8.3 Nucleic Acid Isolation and Preparation; 3.9 Fields of PCR Application; 3.9.1 Sequencing; 3.9.1.1 Pyrosequencing; 3.9.2 Typing and Epidemiology; 3.9.3 Pathogen Detection in Complex Clinical Samples; 3.9.4 General Aspects of Result Interpretation; 3.9.4.1 Sensitivity and Specificity; 3.9.4.2 NAT versus Culture 3.9.4.3 The Fate of Antibiotic Resistances Detection |
| Record Nr. | UNINA-9910132281803321 |
| Weinheim, Germany : , : Wiley Blackwell, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Modern techniques for pathogen detection / / edited by Jürgen Popp and Michael Bauer
| Modern techniques for pathogen detection / / edited by Jürgen Popp and Michael Bauer |
| Pubbl/distr/stampa | Weinheim, Germany : , : Wiley Blackwell, , 2015 |
| Descrizione fisica | 1 online resource (353 p.) |
| Disciplina | 616.9 |
| Soggetto topico | Pathogenic microorganisms - Detection |
| ISBN |
3-527-68798-X
3-527-68797-1 3-527-68799-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Contents; Preface; List of Contributors; Chapter 1 Unmet Medical Needs in Life-Threatening Infections - Caring for the Critically Ill; 1.1 Life Threatening Infections and Sepsis - Defining the Problem; 1.2 Sepsis as a ""Hidden Healthcare Disaster""; 1.3 Microorganisms and Types of Infection Triggering Sepsis; 1.4 Emerging Problems Related to Resistance in Bacterial Infections; 1.5 The Role of Fungi and Viruses; 1.6 The Need for New Approaches in Diagnostics of Life-Threatening Infection and Sepsis
1.7 Rapid and Sensitive Culture-Independent Strategies to Identify Blood Stream Infection1.8 Beyond Infection - Profiling the Immune Response of the Septic Host; 1.9 Host Factors Contributing to Pathogenesis of Sepsis; References; Chapter 2 Identification Methods - An Overview; 2.1 Taxonomy of Pathogenic Organisms; 2.2 Microscopic Methods; 2.2.1 Stains; 2.2.2 Autofluorescence; 2.2.3 Immunofluorescence; 2.2.4 In Situ Hybridization; 2.2.5 Dark-Field Microscopy; 2.3 Culture-Based Methods; 2.3.1 Blood Culture; 2.3.2 Automated Differentiation Systems; 2.3.3 API System; 2.3.4 Selective Agar 2.3.5 Susceptibility Testing2.3.6 Other Methods; 2.4 Nucleic Acid-Based Techniques; 2.4.1 PCR; 2.4.1.1 Real-Time PCR; 2.4.1.2 Multiplex-PCR; 2.4.1.3 RAPD and rep-PCR; 2.4.1.4 Microarrays; 2.4.1.5 RFLP; 2.4.2 Sequencing; 2.4.2.1 Ribosomal RNA Genes; 2.4.2.2 MLST; 2.4.2.3 NG Sequencing; 2.5 Serology; 2.5.1 Antigen-Antibody-Based Methods; 2.5.1.1 Agglutination; 2.5.1.2 Immunodiffusion; 2.5.1.3 Quantitative Immunoassays; 2.5.2 Automated Immunoassays; 2.5.3 Applications of Serological Test; 2.5.3.1 Types of Human Antibodies and What They Indicate? 2.5.3.2 Validity of Antibodies against Opportunistic Species2.5.3.3 Specificity of Fungal Antigens; 2.5.3.4 Specific Urine Antigen Tests; 2.5.4 Biomarkers; 2.6 Conclusions and Perspectives; References; Chapter 3 Nucleic Acid Amplification Techniques; 3.1 Introduction; 3.2 The Basic PCR Protocol; 3.3 Primer Design; 3.3.1 Specific versus Broad-Range Primers and the Fate of False Targeting; 3.4 Modified End-Point PCR Protocols; 3.5 Non-PCR NAT: Isothermal Amplification Protocols; 3.6 Quantitative PCR; 3.6.1 Quantification in qPCR; 3.6.2 Melting Curve Analysis (MCA) 3.7 Controls, Probing, and General Aspects of Result Interpretation3.7.1 Strategies in Amplicon Verification; 3.7.1.1 (DNA) Microarray; 3.7.1.2 Flow Cytometry; 3.7.1.3 Mass Spectrometry; 3.7.2 PCR Inhibitors; 3.8 Preanalytics; 3.8.1 Sample Volume; 3.8.2 Cell Lysis; 3.8.3 Nucleic Acid Isolation and Preparation; 3.9 Fields of PCR Application; 3.9.1 Sequencing; 3.9.1.1 Pyrosequencing; 3.9.2 Typing and Epidemiology; 3.9.3 Pathogen Detection in Complex Clinical Samples; 3.9.4 General Aspects of Result Interpretation; 3.9.4.1 Sensitivity and Specificity; 3.9.4.2 NAT versus Culture 3.9.4.3 The Fate of Antibiotic Resistances Detection |
| Record Nr. | UNINA-9910827569403321 |
| Weinheim, Germany : , : Wiley Blackwell, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Proceedings of the 1991 ACM SIGSMALL/PC Symposium on Small Systems
| Proceedings of the 1991 ACM SIGSMALL/PC Symposium on Small Systems |
| Autore | Bauer Michael |
| Pubbl/distr/stampa | [Place of publication not identified] : , : ACM Press, , 1991 |
| Descrizione fisica | 1 online resource (106 pages) |
| Collana | ACM Conferences. |
| Soggetto topico |
Microcomputers - Congresses
Minicomputers - Congresses Engineering & Applied Sciences Computer Science |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Altri titoli varianti | SIGSMALL '91 |
| Record Nr. | UNINA-9910376057803321 |
Bauer Michael
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| [Place of publication not identified] : , : ACM Press, , 1991 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||