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Single cell analysis [[electronic resource] ] : technologies and applications / / edited by Dario Anselmetti
| Single cell analysis [[electronic resource] ] : technologies and applications / / edited by Dario Anselmetti |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2009 |
| Descrizione fisica | 1 online resource (286 p.) |
| Disciplina | 571.6 |
| Altri autori (Persone) | AnselmettiD |
| Soggetto topico |
Cells - Analysis
Cytology |
| ISBN |
1-282-46084-6
9786612460845 3-527-62665-4 3-527-62664-6 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Single Cell Analysis: Technologies and Applications; Contents; Foreword; Preface; List of Contributors; Part I Single Cell Analysis: Imaging; 1 Single Molecule Fluorescence Monitoring in Eukaryotic Cells: Intranuclear Dynamics of Splicing Factors; 1.1 Motivation; 1.2 Experimental Approach; 1.3 Single Particle Tracking within Living Cells; 1.4 Pre-Messenger RNA Splicing; 1.5 Intranuclear Splicing Factor Tracking; 1.6 Intranuclear U1 snRNP Splicing Factor Binding; 1.7 Events in Speckles; 1.8 Intranuclear U1 snRNP Mobility; 1.9 Perspectives of Single Molecule Microscopy; References
2 Gene Classification and Quantitative Analysis of Gene Regulation in Bacteria using Single Cell Atomic Force Microscopy and Single Molecule Force Spectroscopy2.1 Introduction; 2.2 AFM on Paracrystalline Cell Surface Layers of C. glutamicum: Protein Sequence Information and Morphology; 2.3 Imaging of Living C. glutamicum Cells with Molecular Resolution: Genes, Transcriptional Regulation and Morphology; 2.4 Single Molecule Force Spectroscopy on Specific Protein-DNA Complexes: Transcriptional Regulation in S. meliloti 2.5 Effector-Induced Protein-DNA Binding on the Single Molecule Level: Quorum Sensing in S. meliloti2.6 Conclusion; References; 3 Cellular Cryo-Electron Tomography (CET): Towards a Voyage to the Inner Space of Cells; 3.1 Introduction; 3.2 Tomography with the Electron Microscope - a Practical Perspective; 3.2.1 Sample Preparation; 3.2.2 Instrumental and Technical Requirements; 3.2.3 Alignment, Reconstruction and Visualization; 3.3 Molecular Interpretation of Cellular Tomograms; 3.4 Outlook: The Future is Bright; References; Part II Single Cell Analysis: Technologies; 4 Single Cell Proteomics 4.1 Introduction4.2 The Challenge; 4.3 Single Cell Proteomics: Mass Spectrometry; 4.4 Single Cell Separations; 4.5 Ultrasensitive Protein Analysis: Capillary Electrophoresis with Laser-Induced Fluorescence Detection; 4.6 Capillary Sieving Electrophoresis of Proteins from a Single Cancer Cell; 4.7 Cell Cycle-dependent Single Cell Capillary Sieving Electrophoresis; 4.8 Tentative Identification of Proteins in Single Cell Electropherograms; 4.9 Capillary Micellar and Submicellar Separation of Proteins from a Single Cell; 4.10 Two-Dimensional Capillary Electrophoresis of Proteins in a Single Cell 4.11 Single Copy Detection of Specific Proteins in Single Cells4.12 Conclusion; References; 5 Protein Analysis of Single Cells in Microfluidic Format; 5.1 Introduction; 5.2 Microfluidic Single Cell Analysis Concept; 5.2.1 Single Cell Selection and Trapping; 5.2.2 Single Cell Lysis; 5.3 Single Cell Electrophoretic Separation and Detection of Proteins; 5.3.1 Label-Based Fluorescence Detection; 5.3.2 Label-Free Fluorescence Detection; 5.3.2.1 UV-LIF in Quartz Microfluidic Devices; 5.3.2.2 UV-LIF in PDMS Microfluidic Devices; 5.3.2.3 Single Cell UV-LIF Electrophoretic Analysis 5.4 Future Directions in Single Cell Analysis |
| Record Nr. | UNINA-9910140015103321 |
| Weinheim, : Wiley-VCH, c2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Single cell analysis [[electronic resource] ] : technologies and applications / / edited by Dario Anselmetti
| Single cell analysis [[electronic resource] ] : technologies and applications / / edited by Dario Anselmetti |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2009 |
| Descrizione fisica | 1 online resource (286 p.) |
| Disciplina | 571.6 |
| Altri autori (Persone) | AnselmettiD |
| Soggetto topico |
Cells - Analysis
Cytology |
| ISBN |
1-282-46084-6
9786612460845 3-527-62665-4 3-527-62664-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Single Cell Analysis: Technologies and Applications; Contents; Foreword; Preface; List of Contributors; Part I Single Cell Analysis: Imaging; 1 Single Molecule Fluorescence Monitoring in Eukaryotic Cells: Intranuclear Dynamics of Splicing Factors; 1.1 Motivation; 1.2 Experimental Approach; 1.3 Single Particle Tracking within Living Cells; 1.4 Pre-Messenger RNA Splicing; 1.5 Intranuclear Splicing Factor Tracking; 1.6 Intranuclear U1 snRNP Splicing Factor Binding; 1.7 Events in Speckles; 1.8 Intranuclear U1 snRNP Mobility; 1.9 Perspectives of Single Molecule Microscopy; References
2 Gene Classification and Quantitative Analysis of Gene Regulation in Bacteria using Single Cell Atomic Force Microscopy and Single Molecule Force Spectroscopy2.1 Introduction; 2.2 AFM on Paracrystalline Cell Surface Layers of C. glutamicum: Protein Sequence Information and Morphology; 2.3 Imaging of Living C. glutamicum Cells with Molecular Resolution: Genes, Transcriptional Regulation and Morphology; 2.4 Single Molecule Force Spectroscopy on Specific Protein-DNA Complexes: Transcriptional Regulation in S. meliloti 2.5 Effector-Induced Protein-DNA Binding on the Single Molecule Level: Quorum Sensing in S. meliloti2.6 Conclusion; References; 3 Cellular Cryo-Electron Tomography (CET): Towards a Voyage to the Inner Space of Cells; 3.1 Introduction; 3.2 Tomography with the Electron Microscope - a Practical Perspective; 3.2.1 Sample Preparation; 3.2.2 Instrumental and Technical Requirements; 3.2.3 Alignment, Reconstruction and Visualization; 3.3 Molecular Interpretation of Cellular Tomograms; 3.4 Outlook: The Future is Bright; References; Part II Single Cell Analysis: Technologies; 4 Single Cell Proteomics 4.1 Introduction4.2 The Challenge; 4.3 Single Cell Proteomics: Mass Spectrometry; 4.4 Single Cell Separations; 4.5 Ultrasensitive Protein Analysis: Capillary Electrophoresis with Laser-Induced Fluorescence Detection; 4.6 Capillary Sieving Electrophoresis of Proteins from a Single Cancer Cell; 4.7 Cell Cycle-dependent Single Cell Capillary Sieving Electrophoresis; 4.8 Tentative Identification of Proteins in Single Cell Electropherograms; 4.9 Capillary Micellar and Submicellar Separation of Proteins from a Single Cell; 4.10 Two-Dimensional Capillary Electrophoresis of Proteins in a Single Cell 4.11 Single Copy Detection of Specific Proteins in Single Cells4.12 Conclusion; References; 5 Protein Analysis of Single Cells in Microfluidic Format; 5.1 Introduction; 5.2 Microfluidic Single Cell Analysis Concept; 5.2.1 Single Cell Selection and Trapping; 5.2.2 Single Cell Lysis; 5.3 Single Cell Electrophoretic Separation and Detection of Proteins; 5.3.1 Label-Based Fluorescence Detection; 5.3.2 Label-Free Fluorescence Detection; 5.3.2.1 UV-LIF in Quartz Microfluidic Devices; 5.3.2.2 UV-LIF in PDMS Microfluidic Devices; 5.3.2.3 Single Cell UV-LIF Electrophoretic Analysis 5.4 Future Directions in Single Cell Analysis |
| Record Nr. | UNINA-9910830701603321 |
| Weinheim, : Wiley-VCH, c2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Single cell analysis [[electronic resource] ] : technologies and applications / / edited by Dario Anselmetti
| Single cell analysis [[electronic resource] ] : technologies and applications / / edited by Dario Anselmetti |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2009 |
| Descrizione fisica | 1 online resource (286 p.) |
| Disciplina | 571.6 |
| Altri autori (Persone) | AnselmettiD |
| Soggetto topico |
Cells - Analysis
Cytology |
| ISBN |
1-282-46084-6
9786612460845 3-527-62665-4 3-527-62664-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Single Cell Analysis: Technologies and Applications; Contents; Foreword; Preface; List of Contributors; Part I Single Cell Analysis: Imaging; 1 Single Molecule Fluorescence Monitoring in Eukaryotic Cells: Intranuclear Dynamics of Splicing Factors; 1.1 Motivation; 1.2 Experimental Approach; 1.3 Single Particle Tracking within Living Cells; 1.4 Pre-Messenger RNA Splicing; 1.5 Intranuclear Splicing Factor Tracking; 1.6 Intranuclear U1 snRNP Splicing Factor Binding; 1.7 Events in Speckles; 1.8 Intranuclear U1 snRNP Mobility; 1.9 Perspectives of Single Molecule Microscopy; References
2 Gene Classification and Quantitative Analysis of Gene Regulation in Bacteria using Single Cell Atomic Force Microscopy and Single Molecule Force Spectroscopy2.1 Introduction; 2.2 AFM on Paracrystalline Cell Surface Layers of C. glutamicum: Protein Sequence Information and Morphology; 2.3 Imaging of Living C. glutamicum Cells with Molecular Resolution: Genes, Transcriptional Regulation and Morphology; 2.4 Single Molecule Force Spectroscopy on Specific Protein-DNA Complexes: Transcriptional Regulation in S. meliloti 2.5 Effector-Induced Protein-DNA Binding on the Single Molecule Level: Quorum Sensing in S. meliloti2.6 Conclusion; References; 3 Cellular Cryo-Electron Tomography (CET): Towards a Voyage to the Inner Space of Cells; 3.1 Introduction; 3.2 Tomography with the Electron Microscope - a Practical Perspective; 3.2.1 Sample Preparation; 3.2.2 Instrumental and Technical Requirements; 3.2.3 Alignment, Reconstruction and Visualization; 3.3 Molecular Interpretation of Cellular Tomograms; 3.4 Outlook: The Future is Bright; References; Part II Single Cell Analysis: Technologies; 4 Single Cell Proteomics 4.1 Introduction4.2 The Challenge; 4.3 Single Cell Proteomics: Mass Spectrometry; 4.4 Single Cell Separations; 4.5 Ultrasensitive Protein Analysis: Capillary Electrophoresis with Laser-Induced Fluorescence Detection; 4.6 Capillary Sieving Electrophoresis of Proteins from a Single Cancer Cell; 4.7 Cell Cycle-dependent Single Cell Capillary Sieving Electrophoresis; 4.8 Tentative Identification of Proteins in Single Cell Electropherograms; 4.9 Capillary Micellar and Submicellar Separation of Proteins from a Single Cell; 4.10 Two-Dimensional Capillary Electrophoresis of Proteins in a Single Cell 4.11 Single Copy Detection of Specific Proteins in Single Cells4.12 Conclusion; References; 5 Protein Analysis of Single Cells in Microfluidic Format; 5.1 Introduction; 5.2 Microfluidic Single Cell Analysis Concept; 5.2.1 Single Cell Selection and Trapping; 5.2.2 Single Cell Lysis; 5.3 Single Cell Electrophoretic Separation and Detection of Proteins; 5.3.1 Label-Based Fluorescence Detection; 5.3.2 Label-Free Fluorescence Detection; 5.3.2.1 UV-LIF in Quartz Microfluidic Devices; 5.3.2.2 UV-LIF in PDMS Microfluidic Devices; 5.3.2.3 Single Cell UV-LIF Electrophoretic Analysis 5.4 Future Directions in Single Cell Analysis |
| Record Nr. | UNINA-9910841236303321 |
| Weinheim, : Wiley-VCH, c2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||