Epigenetics and dermatology / / Qianjin Lu, Christopher C. Chang and Bruce C. Richardson ; contributors Nezam Altorok [and fifty two others] |
Autore | Lu Qianjin |
Pubbl/distr/stampa | London, [England] : , : Academic Press, , 2015 |
Descrizione fisica | 1 online resource (525 p.) |
Disciplina | 616.5042 |
Soggetto topico |
Epigenetics
Skin - Diseases Dermatology |
ISBN | 0-12-801272-2 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Front Cover; Epigenetics and Dermatology; Copyright Page; Dedication; Contents; List of Contributors; Preface; Acknowledgments; 1. Biological and Historical Aspects of Epigenetics; 1 Introduction to Epigenetics; References; 2 Laboratory Methods in Epigenetics; 2.1 Introduction; 2.2 DNA Methylation Analysis; 2.2.1 Methods to Distinguish 5-Methylcytosine from Cytosine; 2.2.1.1 Restriction Endonuclease-Based Analysis; 2.2.1.1.1 Southern Blot; 2.2.1.1.2 Methylation-Sensitive Amplified Polymorphism; 2.2.1.2 Bisulfite Conversion Technique and Derivatives; 2.2.1.2.1 Bisulfite Sequencing PCR
2.2.1.2.2 Pyrosequencing2.2.1.2.3 Combined Bisulfite and Restriction Analysis; 2.2.1.2.4 Methylation-Sensitive Single-Nucleotide Primer Extension and SnuPE Ion Pair Reversed-Phase High Performance Liqui...; 2.2.1.2.5 Methylation-Sensitive Melting Curve Analysis; 2.2.1.2.6 Methylation-Sensitive High-Resolution Melting; 2.2.1.2.7 MethyLight; 2.2.1.3 Immunoprecipitation-Based Methods; 2.2.1.3.1 Methylated-CpG Island Recovery Assay; 2.2.1.3.2 Methyl-Binding-PCR; 2.2.1.4 Mass Spectrometry-Based Methods; 2.2.1.4.1 MALDI-TOF Mass Spectrometry with Base-Specific Cleavage 2.2.1.4.2 MALDI-TOF Mass Spectrometry with Primer Extension2.2.2 Genome-Scale DNA Methylation Analysis; 2.2.2.1 Microarray-Based Analysis of DNA Methylation Changes; 2.2.2.1.1 Sample Preparation; 2.2.2.1.2 Microarray Used in DNA Methylation Profiling; 2.2.2.2 Next-Generation Sequencing Techniques; 2.3 Techniques Used for 5hmC Mark Detection; 2.4 Histone Modification Analysis; 2.4.1 Chromatin Immunoprecipitation; 2.4.2 ChIP-on-Chip; 2.4.3 ChIP-seq; 2.4.3.1 Workflow of ChIP-seq; 2.4.3.2 Analysis Pipeline of ChIP-seq Data; 2.4.3.2.1 Read Aligner; 2.4.3.2.2 Peak Calling; 2.4.3.2.3 Motif Finding 2.4.3.3 Advantages of ChIP-seq2.4.4 Challenges for Histone Modification Analysis; 2.5 miRNA Analysis; 2.5.1 miRNA Detection; 2.5.1.1 Microarray; 2.5.1.2 Next-Generation Sequencing; 2.5.1.3 RT-PCR; 2.5.1.4 Northern Blot Analysis; 2.5.1.5 Others; 2.5.2 Target Prediction; 2.5.2.1 Target Scan; 2.5.2.2 PicTar; 2.5.2.3 DIANA-microT; 2.5.2.4 Others; 2.5.3 Target Validation and Functional Analysis; 2.5.3.1 Luciferase Reporter Assays; 2.5.3.2 Gain-of-Function and Loss-of-Function Experiments; 2.6 Conclusion; List of Abbreviations; References; 3 Keratinocyte Differentiation and Epigenetics 3.1 Introduction3.2 Gene Expression in Keratinocyte Differentiation; 3.3 Epigenetic Modulation in Keratinocyte Differentiation; 3.4 Epigenetics and Skin Diseases; 3.5 Conclusion; References; 4 Epigenetics and Fibrosis: Lessons, Challenges, and Windows of Opportunity; 4.1 Introduction; 4.2 Incidence and Prevalence of Fibrosis; 4.3 Biology of Epigenetics; 4.3.1 DNA Methylation; 4.3.2 Histone Modifications; 4.4 Epigenetics and Fibrosis; 4.4.1 Epigenetics and Lung Fibrosis; 4.4.1.1 DNA Methylation and Lung Fibrosis; 4.4.1.2 Histone Modifications and Lung Fibrosis 4.4.2 Epigenetics and Liver Fibrosis |
Record Nr. | UNINA-9910788029503321 |
Lu Qianjin
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London, [England] : , : Academic Press, , 2015 | ||
![]() | ||
Lo trovi qui: Univ. Federico II | ||
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Epigenetics and dermatology / / Qianjin Lu, Christopher C. Chang and Bruce C. Richardson ; contributors Nezam Altorok [and fifty two others] |
Autore | Lu Qianjin |
Pubbl/distr/stampa | London, [England] : , : Academic Press, , 2015 |
Descrizione fisica | 1 online resource (525 p.) |
Disciplina | 616.5042 |
Soggetto topico |
Epigenetics
Skin - Diseases Dermatology |
ISBN | 0-12-801272-2 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Front Cover; Epigenetics and Dermatology; Copyright Page; Dedication; Contents; List of Contributors; Preface; Acknowledgments; 1. Biological and Historical Aspects of Epigenetics; 1 Introduction to Epigenetics; References; 2 Laboratory Methods in Epigenetics; 2.1 Introduction; 2.2 DNA Methylation Analysis; 2.2.1 Methods to Distinguish 5-Methylcytosine from Cytosine; 2.2.1.1 Restriction Endonuclease-Based Analysis; 2.2.1.1.1 Southern Blot; 2.2.1.1.2 Methylation-Sensitive Amplified Polymorphism; 2.2.1.2 Bisulfite Conversion Technique and Derivatives; 2.2.1.2.1 Bisulfite Sequencing PCR
2.2.1.2.2 Pyrosequencing2.2.1.2.3 Combined Bisulfite and Restriction Analysis; 2.2.1.2.4 Methylation-Sensitive Single-Nucleotide Primer Extension and SnuPE Ion Pair Reversed-Phase High Performance Liqui...; 2.2.1.2.5 Methylation-Sensitive Melting Curve Analysis; 2.2.1.2.6 Methylation-Sensitive High-Resolution Melting; 2.2.1.2.7 MethyLight; 2.2.1.3 Immunoprecipitation-Based Methods; 2.2.1.3.1 Methylated-CpG Island Recovery Assay; 2.2.1.3.2 Methyl-Binding-PCR; 2.2.1.4 Mass Spectrometry-Based Methods; 2.2.1.4.1 MALDI-TOF Mass Spectrometry with Base-Specific Cleavage 2.2.1.4.2 MALDI-TOF Mass Spectrometry with Primer Extension2.2.2 Genome-Scale DNA Methylation Analysis; 2.2.2.1 Microarray-Based Analysis of DNA Methylation Changes; 2.2.2.1.1 Sample Preparation; 2.2.2.1.2 Microarray Used in DNA Methylation Profiling; 2.2.2.2 Next-Generation Sequencing Techniques; 2.3 Techniques Used for 5hmC Mark Detection; 2.4 Histone Modification Analysis; 2.4.1 Chromatin Immunoprecipitation; 2.4.2 ChIP-on-Chip; 2.4.3 ChIP-seq; 2.4.3.1 Workflow of ChIP-seq; 2.4.3.2 Analysis Pipeline of ChIP-seq Data; 2.4.3.2.1 Read Aligner; 2.4.3.2.2 Peak Calling; 2.4.3.2.3 Motif Finding 2.4.3.3 Advantages of ChIP-seq2.4.4 Challenges for Histone Modification Analysis; 2.5 miRNA Analysis; 2.5.1 miRNA Detection; 2.5.1.1 Microarray; 2.5.1.2 Next-Generation Sequencing; 2.5.1.3 RT-PCR; 2.5.1.4 Northern Blot Analysis; 2.5.1.5 Others; 2.5.2 Target Prediction; 2.5.2.1 Target Scan; 2.5.2.2 PicTar; 2.5.2.3 DIANA-microT; 2.5.2.4 Others; 2.5.3 Target Validation and Functional Analysis; 2.5.3.1 Luciferase Reporter Assays; 2.5.3.2 Gain-of-Function and Loss-of-Function Experiments; 2.6 Conclusion; List of Abbreviations; References; 3 Keratinocyte Differentiation and Epigenetics 3.1 Introduction3.2 Gene Expression in Keratinocyte Differentiation; 3.3 Epigenetic Modulation in Keratinocyte Differentiation; 3.4 Epigenetics and Skin Diseases; 3.5 Conclusion; References; 4 Epigenetics and Fibrosis: Lessons, Challenges, and Windows of Opportunity; 4.1 Introduction; 4.2 Incidence and Prevalence of Fibrosis; 4.3 Biology of Epigenetics; 4.3.1 DNA Methylation; 4.3.2 Histone Modifications; 4.4 Epigenetics and Fibrosis; 4.4.1 Epigenetics and Lung Fibrosis; 4.4.1.1 DNA Methylation and Lung Fibrosis; 4.4.1.2 Histone Modifications and Lung Fibrosis 4.4.2 Epigenetics and Liver Fibrosis |
Record Nr. | UNINA-9910815148703321 |
Lu Qianjin
![]() |
||
London, [England] : , : Academic Press, , 2015 | ||
![]() | ||
Lo trovi qui: Univ. Federico II | ||
|