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Bacterial toxins : tools in cell biology and pharmacology / / Klaus Aktories (Ed.)
| Bacterial toxins : tools in cell biology and pharmacology / / Klaus Aktories (Ed.) |
| Pubbl/distr/stampa | London, [England] : , : Chapman & Hall, , 1997 |
| Descrizione fisica | 1 online resource (336 p.) |
| Disciplina |
579.3165
615.95293 |
| Collana | Laboratory Companion |
| Soggetto topico | Bacterial toxins |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-84256-7
9786611842567 3-527-61461-3 3-527-61460-5 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Bacterial Toxins; Contents; CHAPTER 1 . Cholera Toxin: Mechanism of Action and Potential Use in Vaccine Development; 1.1 Introduction; 1.2 Molecular Aspects of Cholera Toxin Action; 1.2.1 Structure and Relationship to Other Toxins; 1.2.2 Toxin Entry into Cells and Events Leading to Pathogenesis; 1.2.3 Enzymology of Cholera Toxin; 1.2.4 In Vitro Stimulation of Cholera Toxin Activity by ARF; 1.3 Practical Aspects of Cholera Toxin Use; 1.3.1 Vaccine and Vaccine Development; 1.3.2 Cholera Toxin as a Molecular Tool; 1.4 Summary
CHAPTER 2 . Cholera Toxin and Escherichia coli Heat-labile Enterotoxin: Biochemical Methods for Assessing Enzymatic Activities2.1 Introduction; 2.2 General Information on CT. LT. ARF and Reagents; 2.2.1 Sources, Purification, and Activation of CTA and LTA; 2.2.2 Sources and Purification of ARF; 2.2.3 Reagents and Materials; 2.2.4 Stock Solutions; 2.3 Assay 1 : The Gsa Assay; 2.3.1 Additional Reagents and Materials Required; 2.3.2 Protocol; 2.4 Assay 2: The Agmatine Assay; 2.4.1 Additional Reagents and Materials Required; 2.4.2 Protocol; 2.5 Assay 3: Auto-ADP-ribosylation Assay 2.5.1 Additional Reagents and Materials Required2.5.2 Protocol; 2.6 Assay 4: NAD Glycohydrolase Assay; 2.6.1 Additional Reagents and Materials Required; 2.6.2 Protocol; 2.7 Comments and Considerations; 2.7.1 Appropriate Controls and Analysis of Data; 2.7.1.1 Controls; 2.7.1.2 Data analysis; 2.7.2 Optimization Interfering Substances, Troubleshooting, and Assay; 2.7.2.1 Interfering substances; 2.7.2.2 Troubleshooting; 2.7.2.3 Assay optimization; 2.7.3 Consideration for the Use of ARF; 2.7.3.1 Lipid/Detergent and Nucleotide Requirements; 2.7.3.2 Development of other Assay Conditions CHAPTER 3 . Pertussis Toxin3.1 Introduction; 3.2 Genetic Regulation of Pertussis Toxin Production; 3.3 Biogenesis of Pertussis Toxin; 3.4 Receptor-binding and Translocation; 3.5 ADP-ribosyltransferase Activity and Enzyme Mechanism; 3.6 Biological Activities and Role of Pertussis Toxin in Pathogenesis; CHAPTER 4 . Pertussis Toxin as a Cell Biology Tool; 4.1 Introduction; 4.2 Pertussis Toxin as a Tool to Modify Cellular Functions; 4.2.1 Cell Culture of Bordetella pertussis; 4.2.2 Source of Pertussis Toxin and Preparation of Solution 4.2.3 Treatment of Mammalian Cell Cultures with Pertussis Toxin4.3 Pertussis Toxin as a Tool to Study Cellular Components; 4.3.1 Activation of Pertussis Toxin for in in vitro ADP- ribosylation; 4.3.2 Preparation of Cell Homogenates and Fractions; 4.3.3 ADP-ribosylation of Membrane Proteins by Pertussis Toxin; 4.3.4 ADP-ribosylation of Proteins by Pertussis Toxin; 4.3.5 Preparation of Samples for SDS-PAGE; 4.3.6 Cleavage of ADP-ribose from Ga Subunits; 4.4 SDS-Gel Electrophoresis; 4.5 Reagents and Chemicals; CHAPTER 5 . Clostridium botulinum ADP-ribosyltransferase C3; 5.1 Introduction 5.2 The Family of C3-like Transferases |
| Record Nr. | UNINA-9910144446403321 |
| London, [England] : , : Chapman & Hall, , 1997 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Bacterial toxins : tools in cell biology and pharmacology / / Klaus Aktories (Ed.)
| Bacterial toxins : tools in cell biology and pharmacology / / Klaus Aktories (Ed.) |
| Pubbl/distr/stampa | London, [England] : , : Chapman & Hall, , 1997 |
| Descrizione fisica | 1 online resource (336 p.) |
| Disciplina |
579.3165
615.95293 |
| Collana | Laboratory Companion |
| Soggetto topico | Bacterial toxins |
| ISBN |
1-281-84256-7
9786611842567 3-527-61461-3 3-527-61460-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Bacterial Toxins; Contents; CHAPTER 1 . Cholera Toxin: Mechanism of Action and Potential Use in Vaccine Development; 1.1 Introduction; 1.2 Molecular Aspects of Cholera Toxin Action; 1.2.1 Structure and Relationship to Other Toxins; 1.2.2 Toxin Entry into Cells and Events Leading to Pathogenesis; 1.2.3 Enzymology of Cholera Toxin; 1.2.4 In Vitro Stimulation of Cholera Toxin Activity by ARF; 1.3 Practical Aspects of Cholera Toxin Use; 1.3.1 Vaccine and Vaccine Development; 1.3.2 Cholera Toxin as a Molecular Tool; 1.4 Summary
CHAPTER 2 . Cholera Toxin and Escherichia coli Heat-labile Enterotoxin: Biochemical Methods for Assessing Enzymatic Activities2.1 Introduction; 2.2 General Information on CT. LT. ARF and Reagents; 2.2.1 Sources, Purification, and Activation of CTA and LTA; 2.2.2 Sources and Purification of ARF; 2.2.3 Reagents and Materials; 2.2.4 Stock Solutions; 2.3 Assay 1 : The Gsa Assay; 2.3.1 Additional Reagents and Materials Required; 2.3.2 Protocol; 2.4 Assay 2: The Agmatine Assay; 2.4.1 Additional Reagents and Materials Required; 2.4.2 Protocol; 2.5 Assay 3: Auto-ADP-ribosylation Assay 2.5.1 Additional Reagents and Materials Required2.5.2 Protocol; 2.6 Assay 4: NAD Glycohydrolase Assay; 2.6.1 Additional Reagents and Materials Required; 2.6.2 Protocol; 2.7 Comments and Considerations; 2.7.1 Appropriate Controls and Analysis of Data; 2.7.1.1 Controls; 2.7.1.2 Data analysis; 2.7.2 Optimization Interfering Substances, Troubleshooting, and Assay; 2.7.2.1 Interfering substances; 2.7.2.2 Troubleshooting; 2.7.2.3 Assay optimization; 2.7.3 Consideration for the Use of ARF; 2.7.3.1 Lipid/Detergent and Nucleotide Requirements; 2.7.3.2 Development of other Assay Conditions CHAPTER 3 . Pertussis Toxin3.1 Introduction; 3.2 Genetic Regulation of Pertussis Toxin Production; 3.3 Biogenesis of Pertussis Toxin; 3.4 Receptor-binding and Translocation; 3.5 ADP-ribosyltransferase Activity and Enzyme Mechanism; 3.6 Biological Activities and Role of Pertussis Toxin in Pathogenesis; CHAPTER 4 . Pertussis Toxin as a Cell Biology Tool; 4.1 Introduction; 4.2 Pertussis Toxin as a Tool to Modify Cellular Functions; 4.2.1 Cell Culture of Bordetella pertussis; 4.2.2 Source of Pertussis Toxin and Preparation of Solution 4.2.3 Treatment of Mammalian Cell Cultures with Pertussis Toxin4.3 Pertussis Toxin as a Tool to Study Cellular Components; 4.3.1 Activation of Pertussis Toxin for in in vitro ADP- ribosylation; 4.3.2 Preparation of Cell Homogenates and Fractions; 4.3.3 ADP-ribosylation of Membrane Proteins by Pertussis Toxin; 4.3.4 ADP-ribosylation of Proteins by Pertussis Toxin; 4.3.5 Preparation of Samples for SDS-PAGE; 4.3.6 Cleavage of ADP-ribose from Ga Subunits; 4.4 SDS-Gel Electrophoresis; 4.5 Reagents and Chemicals; CHAPTER 5 . Clostridium botulinum ADP-ribosyltransferase C3; 5.1 Introduction 5.2 The Family of C3-like Transferases |
| Record Nr. | UNISA-996209689703316 |
| London, [England] : , : Chapman & Hall, , 1997 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
Bacterial toxins : tools in cell biology and pharmacology / / Klaus Aktories (Ed.)
| Bacterial toxins : tools in cell biology and pharmacology / / Klaus Aktories (Ed.) |
| Pubbl/distr/stampa | London, [England] : , : Chapman & Hall, , 1997 |
| Descrizione fisica | 1 online resource (336 p.) |
| Disciplina |
579.3165
615.95293 |
| Collana | Laboratory Companion |
| Soggetto topico | Bacterial toxins |
| ISBN |
1-281-84256-7
9786611842567 3-527-61461-3 3-527-61460-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Bacterial Toxins; Contents; CHAPTER 1 . Cholera Toxin: Mechanism of Action and Potential Use in Vaccine Development; 1.1 Introduction; 1.2 Molecular Aspects of Cholera Toxin Action; 1.2.1 Structure and Relationship to Other Toxins; 1.2.2 Toxin Entry into Cells and Events Leading to Pathogenesis; 1.2.3 Enzymology of Cholera Toxin; 1.2.4 In Vitro Stimulation of Cholera Toxin Activity by ARF; 1.3 Practical Aspects of Cholera Toxin Use; 1.3.1 Vaccine and Vaccine Development; 1.3.2 Cholera Toxin as a Molecular Tool; 1.4 Summary
CHAPTER 2 . Cholera Toxin and Escherichia coli Heat-labile Enterotoxin: Biochemical Methods for Assessing Enzymatic Activities2.1 Introduction; 2.2 General Information on CT. LT. ARF and Reagents; 2.2.1 Sources, Purification, and Activation of CTA and LTA; 2.2.2 Sources and Purification of ARF; 2.2.3 Reagents and Materials; 2.2.4 Stock Solutions; 2.3 Assay 1 : The Gsa Assay; 2.3.1 Additional Reagents and Materials Required; 2.3.2 Protocol; 2.4 Assay 2: The Agmatine Assay; 2.4.1 Additional Reagents and Materials Required; 2.4.2 Protocol; 2.5 Assay 3: Auto-ADP-ribosylation Assay 2.5.1 Additional Reagents and Materials Required2.5.2 Protocol; 2.6 Assay 4: NAD Glycohydrolase Assay; 2.6.1 Additional Reagents and Materials Required; 2.6.2 Protocol; 2.7 Comments and Considerations; 2.7.1 Appropriate Controls and Analysis of Data; 2.7.1.1 Controls; 2.7.1.2 Data analysis; 2.7.2 Optimization Interfering Substances, Troubleshooting, and Assay; 2.7.2.1 Interfering substances; 2.7.2.2 Troubleshooting; 2.7.2.3 Assay optimization; 2.7.3 Consideration for the Use of ARF; 2.7.3.1 Lipid/Detergent and Nucleotide Requirements; 2.7.3.2 Development of other Assay Conditions CHAPTER 3 . Pertussis Toxin3.1 Introduction; 3.2 Genetic Regulation of Pertussis Toxin Production; 3.3 Biogenesis of Pertussis Toxin; 3.4 Receptor-binding and Translocation; 3.5 ADP-ribosyltransferase Activity and Enzyme Mechanism; 3.6 Biological Activities and Role of Pertussis Toxin in Pathogenesis; CHAPTER 4 . Pertussis Toxin as a Cell Biology Tool; 4.1 Introduction; 4.2 Pertussis Toxin as a Tool to Modify Cellular Functions; 4.2.1 Cell Culture of Bordetella pertussis; 4.2.2 Source of Pertussis Toxin and Preparation of Solution 4.2.3 Treatment of Mammalian Cell Cultures with Pertussis Toxin4.3 Pertussis Toxin as a Tool to Study Cellular Components; 4.3.1 Activation of Pertussis Toxin for in in vitro ADP- ribosylation; 4.3.2 Preparation of Cell Homogenates and Fractions; 4.3.3 ADP-ribosylation of Membrane Proteins by Pertussis Toxin; 4.3.4 ADP-ribosylation of Proteins by Pertussis Toxin; 4.3.5 Preparation of Samples for SDS-PAGE; 4.3.6 Cleavage of ADP-ribose from Ga Subunits; 4.4 SDS-Gel Electrophoresis; 4.5 Reagents and Chemicals; CHAPTER 5 . Clostridium botulinum ADP-ribosyltransferase C3; 5.1 Introduction 5.2 The Family of C3-like Transferases |
| Record Nr. | UNINA-9910677693003321 |
| London, [England] : , : Chapman & Hall, , 1997 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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The comprehensive sourcebook of bacterial protein toxins / / Joseph Alouf, Institut Pasteur, Paris, France, Daniel Ladant, Institut Pasteur, Unité de Biochimie des Interactions Moléculaires, Paris, France, Michel R. Popoff, Institut Pasteur, Unité des Bactéries anaérobies et Toxines, Paris, France ; contributors, Klaus Aktories [and one hundred ten others]
| The comprehensive sourcebook of bacterial protein toxins / / Joseph Alouf, Institut Pasteur, Paris, France, Daniel Ladant, Institut Pasteur, Unité de Biochimie des Interactions Moléculaires, Paris, France, Michel R. Popoff, Institut Pasteur, Unité des Bactéries anaérobies et Toxines, Paris, France ; contributors, Klaus Aktories [and one hundred ten others] |
| Autore | Alouf J. E (Joseph E.) |
| Edizione | [4th ed.] |
| Pubbl/distr/stampa | Amsterdam, Netherlands : , : Elsevier, , 2015 |
| Descrizione fisica | 1 online resource (1201 p.) |
| Disciplina | 528.2039474 |
| Soggetto topico | Bacterial toxins |
| ISBN |
0-12-800589-0
0-12-800188-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; The Comprehensive Sourcebook of Bacterial Protein Toxins; Copyright Page; Contents; List of Contributors; Introduction to the Fourth Edition; In memory of J. E. Alouf (1929-2014); I. Basic Genomic and Physiological Aspects of Bacterial Protein Toxins; 1 Evolutionary aspects of toxin-producing bacteria; Introduction; Molecular ecology of toxin-producing bacteria; Pathogenicity islands, horizontal gene transfer, and the prevalence of toxins; Toxins encoded by plasmids, bacteriophages, and other pathogenicity islands; PAI-encoded toxins delivered by specialized secretion systems
Molecular evolution of toxins through genetic exchangeGenetic exchange and toxin evolution; Toxin evolution and transmission in the host; Toxin evolution and transmission in the soil environment; Toxin evolution and transmission in aquatic environments; Toxin evolution and transmission in the phyllosphere; Toxin evolution in the guts of insects and other vectors; Toxin evolution in biofilms and regulation by quorum sensing; Vaccines and toxin evolution; Modular recombination of bacterial toxins; Conclusion; References 2 Mobile genetic elements and pathogenicity islands encoding bacterial toxinsIntroduction: The genome structure of prokaryotes; Protein toxins encoded by mobile genetic elements; Protein toxins encoded by plasmids; Gram-negative bacteria; Gram-positive bacteria; Protein toxins encoded by bacteriophages; Gram-negative bacteria; Gram-positive bacteria; Protein toxin genes and other mobile genetic elements; Toxins encoded by PAIs; PAIs; PAI-encoded toxins; Enterobacteria; Other Gram-negative bacteria; The bacterial T6SS; Gram-positive bacteria; Instability of PAIs; Conclusion Evolution of new pathogenic variants caused by PAIs and mobile genetic elementsHGT and the evolution of toxin families; Acknowledgments; References; 3 News and views on protein secretion systems; Introduction; Most historical secretion types; Type I secretion system; Type II secretion system; Type III secretion system; Type IV secretion system: a protein and DNA transport machine; Type V secretion system: free or surface-bound?; Type VI secretion system: weapon for bacterial warfare; How far can we go with secretion types?; Type VII secretion system Extracellular appendages and secretion typesThe type IX secretion system; What about other secretion concepts?; Bacteriocins, colicins, pyocins, contact-dependent inhibition; Cell surface lipoproteins; OM Vesicles; What about Gram-positive bacteria, then?; Closing remarks; References; II. Intracellularly Alive Bacterial Protein Toxins; 4 Diphtheria toxin; Introduction; Diphtheria toxin: from pathology to crystal structure; Symptoms, treatment, prophylaxis, and epidemiology of diphtheria; History of DT research; Regulation of DT production; The structure of DT; The mechanism of action of DT The main steps of cell intoxication |
| Record Nr. | UNINA-9910583473403321 |
Alouf J. E (Joseph E.)
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| Amsterdam, Netherlands : , : Elsevier, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||