On-line LC-NMR and related techniques [[electronic resource] /] / edited by Klaus Albert |
Pubbl/distr/stampa | New York, : J. Wiley & Sons, c2002 |
Descrizione fisica | 1 online resource (308 p.) |
Disciplina |
543.089
543/.0877 |
Altri autori (Persone) | AlbertKlaus |
Soggetto topico |
Nuclear magnetic resonance spectroscopy
Liquid chromatography |
ISBN |
1-280-27011-X
9786610270118 0-470-31452-4 0-470-85481-2 0-470-85482-0 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
On-Line LC-NMR and Related Techniques; Contents; Contributors; Preface; 1 LC-NMR: Theory and Experiment; 1.1 Introduction; 1.2 NMR in a Flowing Liquid; 1.3 Design of Continuous-Flow NMR Probes; 1.4 Experimental Arrangement for HPLC-(1)H NMR Coupling; 1.5 Practical Considerations, Solvent Suppression Techniques, Gradient Elution and Purity of HPLC Solvents; 1.5.1 Solvent Signal Suppression; 1.5.2 Purity of HPLC-Grade Solvents; References; 2 LC-NMR: Automation; 2.1 Practical Use of LC-NMR and LC-NMR/MS; 2.2 Different Working Modes in LC-NMR; 2.2.1 On-Flow; 2.2.2 Direct Stop-Flow
2.2.3 Loop Storage/Loop Transfer2.2.4 Conclusions; 2.3 Use of Mass Spectrometry in the Set-Up; 2.4 Measurement Procedures; 2.4.1 Sample Preparation and Introduction ('Injection') into the Chromatography System; 2.4.2 Chromatographic Separation; 2.4.3 Peak Detection and Selection; 2.4.4 Mass Spectrometric Measurements; 2.4.5 Nuclear Magnetic Resonance Measurements; 2.4.6 Sample Recovery; 2.5 Conclusions; References; 3 Biomedical and Pharmaceutical Applications of HPLC-NMR and HPLC-NMR-MS; 3.1 Introduction; 3.2 Technical and Operational Overview; 3.3 Applications in Combinatorial Chemistry 3.4 Application to Chemical Impurities3.5 Application to Chiral Separations of Pharmaceutical Mixtures; 3.6 Application to Natural Products; 3.7 Application to Chemical Reactivity of Drug Glucuronides; 3.8 Application to Futile Deacetylation Reactions; 3.9 Application to Trapping of Reactive Intermediates; 3.10 Application to Uptake and Transformation of Xenobiotics by Plants; 3.11 Separation of Lipoproteins and their Characterisation using HPLC-NMR; 3.12 Superheated-Water HPLC-NMR and HPLC-NMR-MS Studies on Pharmaceuticals 3.13 Application of Hypernation to a Mixture of Non-Steroidal Anti-Inflammatory Drugs3.14 Concluding Remarks; References; 4 Application of On-Line LC-NMR and Related Techniques to Drug Metabolism Studies; 4.1 Introduction; 4.2 LC-NMR Techniques; 4.2.1 Continuous-Flow LC-NMR; 4.2.2 Time-Slice LC-NMR; 4.2.3 Stop-Flow LC-NMR; 4.2.4 Loop-Storage; 4.2.5 LC-NMR-MS; 4.3 Application of LC-NMR-MS to Drug Metabolism: The Structure Elucidation of Rat Urinary Metabolites of Efavirenz by LC-NMR-MS; 4.3.1 Experimental; 4.3.2 Results; 4.4 Conclusions; References; 5 LC-NMR for Natural Products Analysis 5.1 Application of LC-NMR and LC-NMR-MS Hyphenation to Natural Products Analysis5.1.1 Introduction; 5.1.1.1 General Aspects; 5.1.1.2 Applications; 5.1.2 Application of LC-NMR-MS to Glycosidic Natural Products of Marine Origin; 5.1.2.1 Introduction - Need for LC-NMR; 5.1.2.2 Methodology: On-Flow LC-NMR-MS Screening; 5.1.2.3 NMR - Structural Information; 5.1.2.4 Mass Spectrometry and D-H Back-Exchange Experiments; 5.1.2.5 Stop-Flow Experiments; 5.1.2.6 Complimentary Structural Information of NMR and MS; 5.1.2.7 Conclusions; 5.1.3 Acknowledgements; References 5.2 Hyphenation of Modern Extraction Techniques to LC-NMR for the Analysis of Geometrical Carotenoid Isomers in Functional Food and Biological Tissues |
Record Nr. | UNINA-9910830799103321 |
New York, : J. Wiley & Sons, c2002 | ||
![]() | ||
Lo trovi qui: Univ. Federico II | ||
|
On-line LC-NMR and related techniques [[electronic resource] /] / edited by Klaus Albert |
Pubbl/distr/stampa | New York, : J. Wiley & Sons, c2002 |
Descrizione fisica | 1 online resource (308 p.) |
Disciplina |
543.089
543/.0877 |
Altri autori (Persone) | AlbertKlaus |
Soggetto topico |
Nuclear magnetic resonance spectroscopy
Liquid chromatography |
ISBN |
1-280-27011-X
9786610270118 0-470-31452-4 0-470-85481-2 0-470-85482-0 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
On-Line LC-NMR and Related Techniques; Contents; Contributors; Preface; 1 LC-NMR: Theory and Experiment; 1.1 Introduction; 1.2 NMR in a Flowing Liquid; 1.3 Design of Continuous-Flow NMR Probes; 1.4 Experimental Arrangement for HPLC-(1)H NMR Coupling; 1.5 Practical Considerations, Solvent Suppression Techniques, Gradient Elution and Purity of HPLC Solvents; 1.5.1 Solvent Signal Suppression; 1.5.2 Purity of HPLC-Grade Solvents; References; 2 LC-NMR: Automation; 2.1 Practical Use of LC-NMR and LC-NMR/MS; 2.2 Different Working Modes in LC-NMR; 2.2.1 On-Flow; 2.2.2 Direct Stop-Flow
2.2.3 Loop Storage/Loop Transfer2.2.4 Conclusions; 2.3 Use of Mass Spectrometry in the Set-Up; 2.4 Measurement Procedures; 2.4.1 Sample Preparation and Introduction ('Injection') into the Chromatography System; 2.4.2 Chromatographic Separation; 2.4.3 Peak Detection and Selection; 2.4.4 Mass Spectrometric Measurements; 2.4.5 Nuclear Magnetic Resonance Measurements; 2.4.6 Sample Recovery; 2.5 Conclusions; References; 3 Biomedical and Pharmaceutical Applications of HPLC-NMR and HPLC-NMR-MS; 3.1 Introduction; 3.2 Technical and Operational Overview; 3.3 Applications in Combinatorial Chemistry 3.4 Application to Chemical Impurities3.5 Application to Chiral Separations of Pharmaceutical Mixtures; 3.6 Application to Natural Products; 3.7 Application to Chemical Reactivity of Drug Glucuronides; 3.8 Application to Futile Deacetylation Reactions; 3.9 Application to Trapping of Reactive Intermediates; 3.10 Application to Uptake and Transformation of Xenobiotics by Plants; 3.11 Separation of Lipoproteins and their Characterisation using HPLC-NMR; 3.12 Superheated-Water HPLC-NMR and HPLC-NMR-MS Studies on Pharmaceuticals 3.13 Application of Hypernation to a Mixture of Non-Steroidal Anti-Inflammatory Drugs3.14 Concluding Remarks; References; 4 Application of On-Line LC-NMR and Related Techniques to Drug Metabolism Studies; 4.1 Introduction; 4.2 LC-NMR Techniques; 4.2.1 Continuous-Flow LC-NMR; 4.2.2 Time-Slice LC-NMR; 4.2.3 Stop-Flow LC-NMR; 4.2.4 Loop-Storage; 4.2.5 LC-NMR-MS; 4.3 Application of LC-NMR-MS to Drug Metabolism: The Structure Elucidation of Rat Urinary Metabolites of Efavirenz by LC-NMR-MS; 4.3.1 Experimental; 4.3.2 Results; 4.4 Conclusions; References; 5 LC-NMR for Natural Products Analysis 5.1 Application of LC-NMR and LC-NMR-MS Hyphenation to Natural Products Analysis5.1.1 Introduction; 5.1.1.1 General Aspects; 5.1.1.2 Applications; 5.1.2 Application of LC-NMR-MS to Glycosidic Natural Products of Marine Origin; 5.1.2.1 Introduction - Need for LC-NMR; 5.1.2.2 Methodology: On-Flow LC-NMR-MS Screening; 5.1.2.3 NMR - Structural Information; 5.1.2.4 Mass Spectrometry and D-H Back-Exchange Experiments; 5.1.2.5 Stop-Flow Experiments; 5.1.2.6 Complimentary Structural Information of NMR and MS; 5.1.2.7 Conclusions; 5.1.3 Acknowledgements; References 5.2 Hyphenation of Modern Extraction Techniques to LC-NMR for the Analysis of Geometrical Carotenoid Isomers in Functional Food and Biological Tissues |
Record Nr. | UNINA-9910840935503321 |
New York, : J. Wiley & Sons, c2002 | ||
![]() | ||
Lo trovi qui: Univ. Federico II | ||
|
A practical handbook of preparative HPLC [[electronic resource] /] / Donald A. Wellings |
Autore | Wellings Donald A |
Pubbl/distr/stampa | Oxford, : Elsevier, 2006 |
Descrizione fisica | 1 online resource (193 p.) |
Disciplina | 543.84 |
Soggetto topico |
High performance liquid chromatography
Liquid chromatography |
Soggetto genere / forma | Electronic books. |
ISBN |
1-280-63005-1
9786610630059 0-08-045885-8 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Cover; copyright; Contents; front matter; Preface; Foreword; Abbreviations; body; 1 The history and development of preparative HPLC; 2 Fluid dynamics, mass transport and friction; 3 Modes of chromatographic separation; Particle size; Pore structure, size and surface area; 3.1 Normal phase chromatography; 3.2 Reversed phase chromatography; 3.3 Chiral separations; Chirality; Optical purity; Separation of enantiomers; 3.4 Ion exchange chromatography; 3.5 Exclusion chromatography; 3.6 Affinity chromatography; 4 How to get started; 4.1 Packing a column; Column packing protocol for column type 1
Slurry concentrationSlurry and column preparation; Column packing protocol for column type 2; 4.2 What and where!; 4.3 Product recovery; 4.4 Productivity; 5 Process development and optimization; 5.1 Sample self displacement for purification of a peptide; Analytical HPLC; Gradient selection; Loading study; Flow rate optimization; 5.2 Boxcar injections for chiral separations; 6 Documentation and record keeping; 6.1 Equipment qualification; 6.2 Process documentation; back matter; Appendices; PROCESS INSTRUCTIONS; CONTENTS; DEVIATION SHEET; OPERATION 1 PREPARATION SECTION; 1.01 Abbreviations 1.02 Target Specifications1.03 Raw Materials; 1.04 Equipment; 1.05 Consumables; 1.06 In-Process Tests; Analytical HPLC Conditions; Analytical HPLC Strength Method; 1.07 Ancillary SOPs; 1.08 Cleaning; 1.09 Personnel and Responsibilities; 1.10 Safety; Safety Precautions; OPERATION 2 PRE-PURIFICATION COLUMN PREPARATION; OPERATION 3 PURIFICATION; PURIFICATION DEVIATION SHEET; EXAMPLE OF FRACTION RECONCILIATION DOCUMENT; OPERATION 1 SUMMARY; References; CHAPTER 1; CHAPTER 2; CHAPTER 3; CHAPTER 4; CHAPTER 5; CHAPTER 6; Index |
Record Nr. | UNINA-9910450992603321 |
Wellings Donald A
![]() |
||
Oxford, : Elsevier, 2006 | ||
![]() | ||
Lo trovi qui: Univ. Federico II | ||
|
A practical handbook of preparative HPLC [[electronic resource] /] / Donald A. Wellings |
Autore | Wellings Donald A |
Pubbl/distr/stampa | Oxford, : Elsevier, 2006 |
Descrizione fisica | 1 online resource (193 p.) |
Disciplina | 543.84 |
Soggetto topico |
High performance liquid chromatography
Liquid chromatography |
ISBN |
1-280-63005-1
9786610630059 0-08-045885-8 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Cover; copyright; Contents; front matter; Preface; Foreword; Abbreviations; body; 1 The history and development of preparative HPLC; 2 Fluid dynamics, mass transport and friction; 3 Modes of chromatographic separation; Particle size; Pore structure, size and surface area; 3.1 Normal phase chromatography; 3.2 Reversed phase chromatography; 3.3 Chiral separations; Chirality; Optical purity; Separation of enantiomers; 3.4 Ion exchange chromatography; 3.5 Exclusion chromatography; 3.6 Affinity chromatography; 4 How to get started; 4.1 Packing a column; Column packing protocol for column type 1
Slurry concentrationSlurry and column preparation; Column packing protocol for column type 2; 4.2 What and where!; 4.3 Product recovery; 4.4 Productivity; 5 Process development and optimization; 5.1 Sample self displacement for purification of a peptide; Analytical HPLC; Gradient selection; Loading study; Flow rate optimization; 5.2 Boxcar injections for chiral separations; 6 Documentation and record keeping; 6.1 Equipment qualification; 6.2 Process documentation; back matter; Appendices; PROCESS INSTRUCTIONS; CONTENTS; DEVIATION SHEET; OPERATION 1 PREPARATION SECTION; 1.01 Abbreviations 1.02 Target Specifications1.03 Raw Materials; 1.04 Equipment; 1.05 Consumables; 1.06 In-Process Tests; Analytical HPLC Conditions; Analytical HPLC Strength Method; 1.07 Ancillary SOPs; 1.08 Cleaning; 1.09 Personnel and Responsibilities; 1.10 Safety; Safety Precautions; OPERATION 2 PRE-PURIFICATION COLUMN PREPARATION; OPERATION 3 PURIFICATION; PURIFICATION DEVIATION SHEET; EXAMPLE OF FRACTION RECONCILIATION DOCUMENT; OPERATION 1 SUMMARY; References; CHAPTER 1; CHAPTER 2; CHAPTER 3; CHAPTER 4; CHAPTER 5; CHAPTER 6; Index |
Record Nr. | UNINA-9910784017303321 |
Wellings Donald A
![]() |
||
Oxford, : Elsevier, 2006 | ||
![]() | ||
Lo trovi qui: Univ. Federico II | ||
|
A practical handbook of preparative HPLC / / Donald A. Wellings |
Autore | Wellings Donald A |
Edizione | [1st ed.] |
Pubbl/distr/stampa | Oxford, : Elsevier, 2006 |
Descrizione fisica | 1 online resource (193 p.) |
Disciplina | 543.84 |
Soggetto topico |
High performance liquid chromatography
Liquid chromatography |
ISBN |
1-280-63005-1
9786610630059 0-08-045885-8 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Cover; copyright; Contents; front matter; Preface; Foreword; Abbreviations; body; 1 The history and development of preparative HPLC; 2 Fluid dynamics, mass transport and friction; 3 Modes of chromatographic separation; Particle size; Pore structure, size and surface area; 3.1 Normal phase chromatography; 3.2 Reversed phase chromatography; 3.3 Chiral separations; Chirality; Optical purity; Separation of enantiomers; 3.4 Ion exchange chromatography; 3.5 Exclusion chromatography; 3.6 Affinity chromatography; 4 How to get started; 4.1 Packing a column; Column packing protocol for column type 1
Slurry concentrationSlurry and column preparation; Column packing protocol for column type 2; 4.2 What and where!; 4.3 Product recovery; 4.4 Productivity; 5 Process development and optimization; 5.1 Sample self displacement for purification of a peptide; Analytical HPLC; Gradient selection; Loading study; Flow rate optimization; 5.2 Boxcar injections for chiral separations; 6 Documentation and record keeping; 6.1 Equipment qualification; 6.2 Process documentation; back matter; Appendices; PROCESS INSTRUCTIONS; CONTENTS; DEVIATION SHEET; OPERATION 1 PREPARATION SECTION; 1.01 Abbreviations 1.02 Target Specifications1.03 Raw Materials; 1.04 Equipment; 1.05 Consumables; 1.06 In-Process Tests; Analytical HPLC Conditions; Analytical HPLC Strength Method; 1.07 Ancillary SOPs; 1.08 Cleaning; 1.09 Personnel and Responsibilities; 1.10 Safety; Safety Precautions; OPERATION 2 PRE-PURIFICATION COLUMN PREPARATION; OPERATION 3 PURIFICATION; PURIFICATION DEVIATION SHEET; EXAMPLE OF FRACTION RECONCILIATION DOCUMENT; OPERATION 1 SUMMARY; References; CHAPTER 1; CHAPTER 2; CHAPTER 3; CHAPTER 4; CHAPTER 5; CHAPTER 6; Index |
Record Nr. | UNINA-9910810963503321 |
Wellings Donald A
![]() |
||
Oxford, : Elsevier, 2006 | ||
![]() | ||
Lo trovi qui: Univ. Federico II | ||
|
Practical high-performance liquid chromatography [[electronic resource] /] / Veronika R. Meyer |
Autore | Meyer Veronika |
Edizione | [5th ed.] |
Pubbl/distr/stampa | Hoboken, N.J., : Wiley, 2010 |
Descrizione fisica | 1 online resource (428 p.) |
Disciplina |
543.84
543/.84 |
Soggetto topico |
High performance liquid chromatography
Liquid chromatography |
Soggetto genere / forma | Electronic books. |
ISBN |
1-118-68134-7
1-282-55055-1 9786612550553 0-470-68842-4 0-470-68843-2 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Practical High-Performance Liquid Chromatography; Contents; Preface to the Fifth Edition; Important and Useful Equations for HPLC; 1 Introduction; 1.1 HPLC: A POWERFUL SEPARATION METHOD; 1.2 A FIRST HPLC EXPERIMENT; 1.3 LIQUID CHROMATOGRAPHIC SEPARATION MODES; 1.4 THE HPLC INSTRUMENT; 1.5 SAFETY IN THE HPLC LABORATORY; 1.6 COMPARISON BETWEEN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND GAS CHROMATOGRAPHY; 1.7 COMPARISON BETWEEN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS; 1.8 UNITS FOR PRESSURE, LENGTH AND VISCOSITY; 1.9 SCIENTIFIC JOURNALS; 1.10 RECOMMENDED BOOKS
2 Theoretical Principles2.1 THE CHROMATOGRAPHIC PROCESS; 2.2 BAND BROADENING; 2.3 THE CHROMATOGRAM AND ITS PURPORT; 2.4 GRAPHICAL REPRESENTATION OF PEAK PAIRS WITH DIFFERENT DEGREE OF RESOLUTION; 2.5 FACTORS AFFECTING RESOLUTION; 2.6 EXTRA-COLUMN VOLUMES (DEAD VOLUMES); 2.7 TAILING; 2.8 PEAK CAPACITY AND STATISTICAL RESOLUTION PROBABILITY; 2.9 EFFECTS OF TEMPERATURE IN HPLC; 2.10 THE LIMITS OF HPLC; 2.11 HOW TO OBTAIN PEAK CAPACITY; 3 Pumps; 3.1 GENERAL REQUIREMENTS; 3.2 THE SHORT-STROKE PISTON PUMP; 3.3 MAINTENANCE AND REPAIR; 3.4 OTHER PUMP DESIGNS 4 Preparation of Equipment up to Sample Injection4.1 SELECTION OF THE MOBILE PHASE; 4.2 PREPARATION OF THE MOBILE PHASE; 4.3 GRADIENT SYSTEMS; 4.4 CAPILLARY TUBING; 4.5 FITTINGS; 4.6 SAMPLE INJECTORS; 4.7 SAMPLE SOLUTION AND SAMPLE VOLUME; 5 Solvent Properties; 5.1 TABLE OF ORGANIC SOLVENTS; 5.2 SOLVENT SELECTIVITY; 5.3 MISCIBILITY; 5.4 BUFFERS; 5.5 SHELF LIFE OF MOBILE PHASES; 5.6 THE MIXING CROSS; 6 Detectors; 6.1 GENERAL; 6.2 UV DETECTORS; 6.3 REFRACTIVE INDEX DETECTORS; 6.4 FLUORESCENCE DETECTORS; 6.5 ELECTROCHEMICAL (AMPEROMETRIC) DETECTORS; 6.6 LIGHT-SCATTERING DETECTORS 6.7 OTHER DETECTORS6.8 MULTIPLE DETECTION; 6.9 INDIRECT DETECTION; 6.10 COUPLING WITH SPECTROSCOPY; 7 Columns and Stationary Phases; 7.1 COLUMNS FOR HPLC; 7.2 PRECOLUMNS; 7.3 GENERAL PROPERTIES OF STATIONARY PHASES; 7.4 SILICA; 7.5 CHEMICALLY MODIFIED SILICA; 7.6 STYRENE-DIVINYLBENZENE; 7.7 SOME OTHER STATIONARY PHASES; 7.8 COLUMN CARE AND REGENERATION; 8 HPLC Column Tests; 8.1 SIMPLE TESTS FOR HPLC COLUMNS; 8.2 DETERMINATION OF PARTICLE SIZE; 8.3 DETERMINATION OF BREAKTHROUGH TIME; 8.4 THE TEST MIXTURE; 8.5 DIMENSIONLESS PARAMETERS FOR HPLC COLUMN CHARACTERIZATION 8.6 THE VAN DEEMTER EQUATION FROM REDUCED PARAMETERS AND ITS USE IN COLUMN DIAGNOSIS8.7 VAN DEEMTER CURVES AND OTHER COHERENCES; 8.8 DIFFUSION COEFFICIENTS; 9 Adsorption Chromatography: Normal-Phase Chromatography; 9.1 WHAT IS ADSORPTION?; 9.2 THE ELUOTROPIC SERIES; 9.3 SELECTIVITY PROPERTIES OF THE MOBILE PHASE; 9.4 CHOICE AND OPTIMIZATION OF THE MOBILE PHASE; 9.5 APPLICATIONS; 10 Reversed-Phase Chromatography; 10.1 PRINCIPLE; 10.2 MOBILE PHASES IN REVERSED-PHASE CHROMATOGRAPHY; 10.3 SOLVENT SELECTIVITY AND STRENGTH; 10.4 STATIONARY PHASES 10.5 METHOD DEVELOPMENT IN REVERSED-PHASE CHROMATOGRAPHY |
Record Nr. | UNINA-9910140603603321 |
Meyer Veronika
![]() |
||
Hoboken, N.J., : Wiley, 2010 | ||
![]() | ||
Lo trovi qui: Univ. Federico II | ||
|
Practical high-performance liquid chromatography [[electronic resource] /] / Veronika R. Meyer |
Autore | Meyer Veronika |
Edizione | [5th ed.] |
Pubbl/distr/stampa | Hoboken, N.J., : Wiley, 2010 |
Descrizione fisica | 1 online resource (428 p.) |
Disciplina |
543.84
543/.84 |
Soggetto topico |
High performance liquid chromatography
Liquid chromatography |
ISBN |
1-118-68134-7
1-282-55055-1 9786612550553 0-470-68842-4 0-470-68843-2 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Practical High-Performance Liquid Chromatography; Contents; Preface to the Fifth Edition; Important and Useful Equations for HPLC; 1 Introduction; 1.1 HPLC: A POWERFUL SEPARATION METHOD; 1.2 A FIRST HPLC EXPERIMENT; 1.3 LIQUID CHROMATOGRAPHIC SEPARATION MODES; 1.4 THE HPLC INSTRUMENT; 1.5 SAFETY IN THE HPLC LABORATORY; 1.6 COMPARISON BETWEEN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND GAS CHROMATOGRAPHY; 1.7 COMPARISON BETWEEN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS; 1.8 UNITS FOR PRESSURE, LENGTH AND VISCOSITY; 1.9 SCIENTIFIC JOURNALS; 1.10 RECOMMENDED BOOKS
2 Theoretical Principles2.1 THE CHROMATOGRAPHIC PROCESS; 2.2 BAND BROADENING; 2.3 THE CHROMATOGRAM AND ITS PURPORT; 2.4 GRAPHICAL REPRESENTATION OF PEAK PAIRS WITH DIFFERENT DEGREE OF RESOLUTION; 2.5 FACTORS AFFECTING RESOLUTION; 2.6 EXTRA-COLUMN VOLUMES (DEAD VOLUMES); 2.7 TAILING; 2.8 PEAK CAPACITY AND STATISTICAL RESOLUTION PROBABILITY; 2.9 EFFECTS OF TEMPERATURE IN HPLC; 2.10 THE LIMITS OF HPLC; 2.11 HOW TO OBTAIN PEAK CAPACITY; 3 Pumps; 3.1 GENERAL REQUIREMENTS; 3.2 THE SHORT-STROKE PISTON PUMP; 3.3 MAINTENANCE AND REPAIR; 3.4 OTHER PUMP DESIGNS 4 Preparation of Equipment up to Sample Injection4.1 SELECTION OF THE MOBILE PHASE; 4.2 PREPARATION OF THE MOBILE PHASE; 4.3 GRADIENT SYSTEMS; 4.4 CAPILLARY TUBING; 4.5 FITTINGS; 4.6 SAMPLE INJECTORS; 4.7 SAMPLE SOLUTION AND SAMPLE VOLUME; 5 Solvent Properties; 5.1 TABLE OF ORGANIC SOLVENTS; 5.2 SOLVENT SELECTIVITY; 5.3 MISCIBILITY; 5.4 BUFFERS; 5.5 SHELF LIFE OF MOBILE PHASES; 5.6 THE MIXING CROSS; 6 Detectors; 6.1 GENERAL; 6.2 UV DETECTORS; 6.3 REFRACTIVE INDEX DETECTORS; 6.4 FLUORESCENCE DETECTORS; 6.5 ELECTROCHEMICAL (AMPEROMETRIC) DETECTORS; 6.6 LIGHT-SCATTERING DETECTORS 6.7 OTHER DETECTORS6.8 MULTIPLE DETECTION; 6.9 INDIRECT DETECTION; 6.10 COUPLING WITH SPECTROSCOPY; 7 Columns and Stationary Phases; 7.1 COLUMNS FOR HPLC; 7.2 PRECOLUMNS; 7.3 GENERAL PROPERTIES OF STATIONARY PHASES; 7.4 SILICA; 7.5 CHEMICALLY MODIFIED SILICA; 7.6 STYRENE-DIVINYLBENZENE; 7.7 SOME OTHER STATIONARY PHASES; 7.8 COLUMN CARE AND REGENERATION; 8 HPLC Column Tests; 8.1 SIMPLE TESTS FOR HPLC COLUMNS; 8.2 DETERMINATION OF PARTICLE SIZE; 8.3 DETERMINATION OF BREAKTHROUGH TIME; 8.4 THE TEST MIXTURE; 8.5 DIMENSIONLESS PARAMETERS FOR HPLC COLUMN CHARACTERIZATION 8.6 THE VAN DEEMTER EQUATION FROM REDUCED PARAMETERS AND ITS USE IN COLUMN DIAGNOSIS8.7 VAN DEEMTER CURVES AND OTHER COHERENCES; 8.8 DIFFUSION COEFFICIENTS; 9 Adsorption Chromatography: Normal-Phase Chromatography; 9.1 WHAT IS ADSORPTION?; 9.2 THE ELUOTROPIC SERIES; 9.3 SELECTIVITY PROPERTIES OF THE MOBILE PHASE; 9.4 CHOICE AND OPTIMIZATION OF THE MOBILE PHASE; 9.5 APPLICATIONS; 10 Reversed-Phase Chromatography; 10.1 PRINCIPLE; 10.2 MOBILE PHASES IN REVERSED-PHASE CHROMATOGRAPHY; 10.3 SOLVENT SELECTIVITY AND STRENGTH; 10.4 STATIONARY PHASES 10.5 METHOD DEVELOPMENT IN REVERSED-PHASE CHROMATOGRAPHY |
Record Nr. | UNINA-9910830994003321 |
Meyer Veronika
![]() |
||
Hoboken, N.J., : Wiley, 2010 | ||
![]() | ||
Lo trovi qui: Univ. Federico II | ||
|
Practical high-performance liquid chromatography [[electronic resource] /] / Veronika R. Meyer |
Autore | Meyer Veronika |
Edizione | [5th ed.] |
Pubbl/distr/stampa | Hoboken, N.J., : Wiley, 2010 |
Descrizione fisica | 1 online resource (428 p.) |
Disciplina |
543.84
543/.84 |
Soggetto topico |
High performance liquid chromatography
Liquid chromatography |
ISBN |
1-118-68134-7
1-282-55055-1 9786612550553 0-470-68842-4 0-470-68843-2 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Practical High-Performance Liquid Chromatography; Contents; Preface to the Fifth Edition; Important and Useful Equations for HPLC; 1 Introduction; 1.1 HPLC: A POWERFUL SEPARATION METHOD; 1.2 A FIRST HPLC EXPERIMENT; 1.3 LIQUID CHROMATOGRAPHIC SEPARATION MODES; 1.4 THE HPLC INSTRUMENT; 1.5 SAFETY IN THE HPLC LABORATORY; 1.6 COMPARISON BETWEEN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND GAS CHROMATOGRAPHY; 1.7 COMPARISON BETWEEN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS; 1.8 UNITS FOR PRESSURE, LENGTH AND VISCOSITY; 1.9 SCIENTIFIC JOURNALS; 1.10 RECOMMENDED BOOKS
2 Theoretical Principles2.1 THE CHROMATOGRAPHIC PROCESS; 2.2 BAND BROADENING; 2.3 THE CHROMATOGRAM AND ITS PURPORT; 2.4 GRAPHICAL REPRESENTATION OF PEAK PAIRS WITH DIFFERENT DEGREE OF RESOLUTION; 2.5 FACTORS AFFECTING RESOLUTION; 2.6 EXTRA-COLUMN VOLUMES (DEAD VOLUMES); 2.7 TAILING; 2.8 PEAK CAPACITY AND STATISTICAL RESOLUTION PROBABILITY; 2.9 EFFECTS OF TEMPERATURE IN HPLC; 2.10 THE LIMITS OF HPLC; 2.11 HOW TO OBTAIN PEAK CAPACITY; 3 Pumps; 3.1 GENERAL REQUIREMENTS; 3.2 THE SHORT-STROKE PISTON PUMP; 3.3 MAINTENANCE AND REPAIR; 3.4 OTHER PUMP DESIGNS 4 Preparation of Equipment up to Sample Injection4.1 SELECTION OF THE MOBILE PHASE; 4.2 PREPARATION OF THE MOBILE PHASE; 4.3 GRADIENT SYSTEMS; 4.4 CAPILLARY TUBING; 4.5 FITTINGS; 4.6 SAMPLE INJECTORS; 4.7 SAMPLE SOLUTION AND SAMPLE VOLUME; 5 Solvent Properties; 5.1 TABLE OF ORGANIC SOLVENTS; 5.2 SOLVENT SELECTIVITY; 5.3 MISCIBILITY; 5.4 BUFFERS; 5.5 SHELF LIFE OF MOBILE PHASES; 5.6 THE MIXING CROSS; 6 Detectors; 6.1 GENERAL; 6.2 UV DETECTORS; 6.3 REFRACTIVE INDEX DETECTORS; 6.4 FLUORESCENCE DETECTORS; 6.5 ELECTROCHEMICAL (AMPEROMETRIC) DETECTORS; 6.6 LIGHT-SCATTERING DETECTORS 6.7 OTHER DETECTORS6.8 MULTIPLE DETECTION; 6.9 INDIRECT DETECTION; 6.10 COUPLING WITH SPECTROSCOPY; 7 Columns and Stationary Phases; 7.1 COLUMNS FOR HPLC; 7.2 PRECOLUMNS; 7.3 GENERAL PROPERTIES OF STATIONARY PHASES; 7.4 SILICA; 7.5 CHEMICALLY MODIFIED SILICA; 7.6 STYRENE-DIVINYLBENZENE; 7.7 SOME OTHER STATIONARY PHASES; 7.8 COLUMN CARE AND REGENERATION; 8 HPLC Column Tests; 8.1 SIMPLE TESTS FOR HPLC COLUMNS; 8.2 DETERMINATION OF PARTICLE SIZE; 8.3 DETERMINATION OF BREAKTHROUGH TIME; 8.4 THE TEST MIXTURE; 8.5 DIMENSIONLESS PARAMETERS FOR HPLC COLUMN CHARACTERIZATION 8.6 THE VAN DEEMTER EQUATION FROM REDUCED PARAMETERS AND ITS USE IN COLUMN DIAGNOSIS8.7 VAN DEEMTER CURVES AND OTHER COHERENCES; 8.8 DIFFUSION COEFFICIENTS; 9 Adsorption Chromatography: Normal-Phase Chromatography; 9.1 WHAT IS ADSORPTION?; 9.2 THE ELUOTROPIC SERIES; 9.3 SELECTIVITY PROPERTIES OF THE MOBILE PHASE; 9.4 CHOICE AND OPTIMIZATION OF THE MOBILE PHASE; 9.5 APPLICATIONS; 10 Reversed-Phase Chromatography; 10.1 PRINCIPLE; 10.2 MOBILE PHASES IN REVERSED-PHASE CHROMATOGRAPHY; 10.3 SOLVENT SELECTIVITY AND STRENGTH; 10.4 STATIONARY PHASES 10.5 METHOD DEVELOPMENT IN REVERSED-PHASE CHROMATOGRAPHY |
Record Nr. | UNINA-9910841216603321 |
Meyer Veronika
![]() |
||
Hoboken, N.J., : Wiley, 2010 | ||
![]() | ||
Lo trovi qui: Univ. Federico II | ||
|
Practice of high performance liquid chromatography : applications, equipment, and quantitative analysis / editor, H. Engelhardt, with contributions by K. Aitzetmüller ... [et al.] |
Pubbl/distr/stampa | Berlin : Springer, c1986 |
Descrizione fisica | xii, 461 p. : ill. ; 25 cm |
Disciplina | 543.8 |
Altri autori (Persone) | Engelhardt, Heinz |
Soggetto topico |
Chromatography, high pressure liquid
Liquid chromatography |
ISBN | 3540125892 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNISALENTO-991003546419707536 |
Berlin : Springer, c1986 | ||
![]() | ||
Lo trovi qui: Univ. del Salento | ||
|
Praxis der Hochleistungs-Flüssigchromatographie [[electronic resource] /] / Veronika R. Meyer |
Autore | Meyer Veronika |
Edizione | [9. Aufl.] |
Pubbl/distr/stampa | Weinheim, : Wiley, 2004 |
Descrizione fisica | 1 online resource (368 p.) |
Disciplina |
543.0894
543.84 544.924 |
Soggetto topico | Liquid chromatography |
Soggetto genere / forma | Electronic books. |
ISBN |
1-282-02134-6
9786612021343 3-527-62358-2 3-527-62359-0 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | ger |
Nota di contenuto |
Praxis der Hochleistungs- Flüssigchromatographie; Inhaltsverzeichnis; Vorwort; Vorwort zur neunten Auflage; 0 Wichtige und nützliche Gleichungen für die HPLC; 1 Einleitung; 1.1 HPLC: Eine leistungsfähige Trennmethode; 1.2 Ein erstes HPLC-Experiment; 1.3 Die flüssigchromatographischen Trennverfahren; 1.4 Die HPLC-Apparatur; 1.5 Sicherheit am HPLC-Arbeitsplatz; 1.6 Vergleich von Hochleistungs-Flüssigchromatographie und Gaschromatographie; 1.7 Druckeinheiten; 1.8 Längeneinheiten; 1.9 Wissenschaftliche Zeitschriften; 1.10 Empfehlenswerte Bücher; 2 Theoretische Grundlagen
2.1 Der chromatographische Prozess2.2 Bandenverbreiterung; 2.3 Das Chromatogramm und seine Aussage; 2.4 Graphische Darstellung von Peakpaaren mit verschiedener Auflösung; 2.5 Die Beeinflussung der Auflösung; 2.6 Totvolumina (extra-column-Volumina); 2.7 Tailing; 2.8 Peakkapazität und statistische Auflösungswahrscheinlichkeit; 2.9 Temperatureinflüsse in der HPLC; 2.10 Die Grenzen der HPLC; 3 Pumpen; 3.1 Allgemeines; 3.2 Die Kurzhub-Kolbenpumpe; 3.3 Unterhalt und Reparaturen; 3.4 Andere Pumpentypen; 4 Bereitstellung der Apparatur bis zur Probenaufgabe; 4.1 Auswahl der mobilen Phase 4.2 Vorbereitung der mobilen Phase4.3 Gradientensysteme; 4.4 Kapillarleitungen; 4.5 Fittings; 4.6 Probenaufgabesysteme; 4.7 Probelösung und Probevolumen; 5 Lösungsmitteleigenschaften; 5.1 Tabelle organischer Lösungsmittel; 5.2 Lösungsmittelselektivität; 5.3 Mischbarkeit; 5.4 Puffer; 5.5 Haltbarkeit von mobilen Phasen; 6 Detektoren; 6.1 Allgemeines; 6.2 UV-Detektoren; 6.3 Brechungsindex-Detektoren; 6.4 Fluoreszenz-Detektoren; 6.5 Elektrochemische Detektoren; 6.6 Lichtstreuungs-Detektoren; 6.7 Andere Detektoren; 6.8 Mehrfachdetektion; 6.9 Indirekte Detektion; 6.10 Kopplung mit Spektroskopie 7 Säulen und stationäre Phasen7.1 Säulen für die HPLC; 7.2 Vorsäulen; 7.3 Allgemeines über stationäre Phasen; 7.4 Silicagel; 7.5 Chemisch modifiziertes Silicagel; 7.6 Styrol-Divinylbenzol; 7.7 Einige weitere stationäre Phasen; 7.8 Vorsichtsmaßnahmen und Regeneration; 8 Das Testen von HPLC-Säulen; 8.1 Einfache Tests für HPLC-Säulen; 8.2 Bestimmung der Korngröße; 8.3 Bestimmung der Totzeit; 8.4 Das Testgemisch; 8.5 Dimensionslose Größen zur Charakterisierung von HPLC-Säulen; 8.6 Die van Deemter-Gleichung aus reduzierten Größen und ihre Nützlichkeit für die Säulendiagnose 8.7 Diffusionskoeffizienten9 Adsorptions-Chromatographie; 9.1 Was heißt Adsorption?; 9.2 Die eluotrope Reihe; 9.3 Selektivitätseigenschaften der mobilen Phase; 9.4 Wahl und Optimierung der mobilen Phase; 9.5 Anwendungsbeispiele; 10 Umkehrphasen-Chromatographie; 10.1 Prinzip; 10.2 Mobile Phasen in der Umkehrphasen-Chromatographie; 10.3 Selektivität und Stärke der mobilen Phase; 10.4 Stationäre Phasen; 10.5 Methodenentwicklung in der Umkehrphasen-Chromatographie; 10.6 Anwendungsbeispiele; 10.7 Hydrophobic-Interaction-Chromatographie; 11 Chromatographie mit chemisch gebundenen Phasen 11.1 Einführung |
Record Nr. | UNINA-9910144392903321 |
Meyer Veronika
![]() |
||
Weinheim, : Wiley, 2004 | ||
![]() | ||
Lo trovi qui: Univ. Federico II | ||
|