Proteomic biology using LC-MS [[electronic resource] ] : large scale analysis of cellular dynamics and function / / Nobuhiro Takahashi, Toshiaki Isobe |
Autore | Takahashi Nobuhiro |
Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Interscience, c2008 |
Descrizione fisica | 1 online resource (275 p.) |
Disciplina | 572/.6 |
Altri autori (Persone) | IsobeToshiaki |
Collana | Wiley-Interscience series in mass spectrometry |
Soggetto topico |
Proteomics
Liquid chromatography Mass spectrometry |
ISBN |
1-281-13467-8
9786611134679 0-470-14965-5 0-470-14964-7 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | Overview of proteomics -- Proteomic tools for analysis of cellular dynamics -- Dynamics of functional cellular machinery : from statics to dynamics in proteomic biology. |
Record Nr. | UNINA-9910831066503321 |
Takahashi Nobuhiro
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Hoboken, N.J., : Wiley-Interscience, c2008 | ||
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Lo trovi qui: Univ. Federico II | ||
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Proteomic biology using LC-MS [[electronic resource] ] : large scale analysis of cellular dynamics and function / / Nobuhiro Takahashi, Toshiaki Isobe |
Autore | Takahashi Nobuhiro |
Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Interscience, c2008 |
Descrizione fisica | 1 online resource (275 p.) |
Disciplina | 572/.6 |
Altri autori (Persone) | IsobeToshiaki |
Collana | Wiley-Interscience series in mass spectrometry |
Soggetto topico |
Proteomics
Liquid chromatography Mass spectrometry |
ISBN |
1-281-13467-8
9786611134679 0-470-14965-5 0-470-14964-7 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | Overview of proteomics -- Proteomic tools for analysis of cellular dynamics -- Dynamics of functional cellular machinery : from statics to dynamics in proteomic biology. |
Record Nr. | UNINA-9910841482703321 |
Takahashi Nobuhiro
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Hoboken, N.J., : Wiley-Interscience, c2008 | ||
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Lo trovi qui: Univ. Federico II | ||
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Sample preparation in LC-MS bioanalysis / / edited by Wenkui Li, Wenying Jian, Yunlin Fu |
Pubbl/distr/stampa | Hoboken, NJ : , : Wiley, , 2019 |
Descrizione fisica | 1 online resource (387 pages) |
Disciplina | 543.0894 |
Collana | Wiley Series on Pharmaceutical Science and Biotechnology |
Soggetto topico | Liquid chromatography |
Soggetto genere / forma | Electronic books. |
ISBN |
1-119-27432-X
1-119-27430-3 1-119-27431-1 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNINA-9910555108003321 |
Hoboken, NJ : , : Wiley, , 2019 | ||
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Lo trovi qui: Univ. Federico II | ||
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Sample preparation in LC-MS bioanalysis / / edited by Wenkui Li, Wenying Jian, Yunlin Fu |
Pubbl/distr/stampa | Hoboken, NJ : , : Wiley, , 2019 |
Descrizione fisica | 1 online resource (387 pages) |
Disciplina | 543.0894 |
Collana | Wiley Series on Pharmaceutical Science and Biotechnology |
Soggetto topico | Liquid chromatography |
ISBN |
1-119-27432-X
1-119-27430-3 1-119-27431-1 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNINA-9910677774703321 |
Hoboken, NJ : , : Wiley, , 2019 | ||
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Lo trovi qui: Univ. Federico II | ||
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Separation techniques applied to omics sciences : from principles to relevant applications / / Ana Valéria Colnaghi Simionato |
Autore | Simionato Ana Valéria Colnaghi |
Pubbl/distr/stampa | Cham, Switzerland : , : Springer International Publishing, , [2021] |
Descrizione fisica | 1 online resource (277 pages) |
Disciplina | 660.2842 |
Collana | Advances in Experimental Medicine and Biology |
Soggetto topico |
Separation (Technology) - Data processing
Liquid chromatography Genomes |
Soggetto genere / forma | Llibres electrònics |
ISBN | 3-030-77252-7 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNINA-9910503008203321 |
Simionato Ana Valéria Colnaghi
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Cham, Switzerland : , : Springer International Publishing, , [2021] | ||
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Lo trovi qui: Univ. Federico II | ||
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Target biomarker quantitation by LC-MS / / edited by Dr. Naidong Weng and Dr. Wenying Jian, Janssen Research & Development, LLC |
Edizione | [First edition.] |
Pubbl/distr/stampa | Hoboken, New Jersey : , : Wiley, , 2017 |
Descrizione fisica | 1 online resource |
Disciplina | 572/.36 |
Collana | Wiley Series on Pharmaceutical Science and Biotechnology: Practices, Applications and Methods |
Soggetto topico |
Liquid chromatography
Mass spectrometry Biochemical markers |
Soggetto genere / forma | Electronic books. |
ISBN |
1-119-41306-0
1-119-41307-9 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNINA-9910271010603321 |
Hoboken, New Jersey : , : Wiley, , 2017 | ||
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Lo trovi qui: Univ. Federico II | ||
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Target biomarker quantitation by LC-MS / / edited by Dr. Naidong Weng and Dr. Wenying Jian, Janssen Research & Development, LLC |
Edizione | [First edition.] |
Pubbl/distr/stampa | Hoboken, New Jersey : , : Wiley, , 2017 |
Descrizione fisica | 1 online resource |
Disciplina | 572/.36 |
Collana | Wiley Series on Pharmaceutical Science and Biotechnology: Practices, Applications and Methods |
Soggetto topico |
Liquid chromatography
Mass spectrometry Biochemical markers |
ISBN |
1-119-41306-0
1-119-41307-9 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNINA-9910830658503321 |
Hoboken, New Jersey : , : Wiley, , 2017 | ||
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Lo trovi qui: Univ. Federico II | ||
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Ultra-high performance liquid chromatography and its applications [[electronic resource] /] / edited by Quanyun Alan Xu |
Pubbl/distr/stampa | Hoboken, N.J., : John Wiley & Sons Inc., 2013 |
Descrizione fisica | 1 online resource (306 p.) |
Disciplina | 543/.84 |
Altri autori (Persone) | XuQuanyun A. <1961-> |
Soggetto topico | Liquid chromatography |
ISBN |
1-118-53398-4
1-118-53395-X 1-299-46494-7 1-118-53399-2 |
Classificazione | TEC009010 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Ultra-High Performance Liquid Chromatography and Its Applications; Contents; Preface; Contributors; Chapter 1 UHPLC Method Development; 1.1 INTRODUCTION; 1.2 METHOD DEVELOPMENT; 1.2.1 Gather Sample Information and Define Method Goals; 1.2.2 Scout Columns and Mobile Phases; 1.2.3 Analyze Scouting Results and Select Separation Conditions; 1.2.4 Optimize the Method; 1.2.5 Validate the Method; 1.2.6 Phase-Appropriate Method Development; 1.2.7 Very High Pressure and Frictional Heating in UHPLC; 1.2.8 Relevance of Various Instrumentation to Method Development
1.2.9 Method Resolution and Speed Requirements1.2.10 Example 1.1; 1.2.11 Example 1.2; 1.3 CONVERSION OF AN HPLC METHOD TO UHPLC; 1.3.1 Select a UHPLC Column with the Same Chemistry; 1.3.2 Measure Dwell Volumes of HPLC and UHPLC Systems; 1.3.3 Calculate Injection Volume, Flow Rate, Holding Time, and Gradient Segment Time; 1.3.4 Perform Verification Experiment; 1.3.5 Validate the Method; 1.3.6 Example 1.3; 1.3.7 Example 1.4; 1.4 CONVERSION OF A UHPLC METHOD TO HPLC; 1.5 SUMMARY; REFERENCES; Chapter 2 Method Transfer Between HPLC and UHPLC Platforms; 2.1 INTRODUCTION 2.2 TRANSFERRING HPLC METHODS TO UHPLC2.2.1 Fundamental Physical Relationships; 2.2.2 Types of UHPLC Applications; 2.2.3 Additional Fundamental Considerations; 2.2.4 Transfer of Isocratic Methods from HPLC to UHPLC; 2.2.5 Cautions on Transferring Isocratic Methods from HPLC to UHPLC; 2.2.6 Transfer of Gradient Methods from HPLC to UHPLC; 2.2.7 Cautions on the Scaling of Gradient Methods from HPLC to UHPLC; 2.2.8 Case Studies and Recommendations; 2.2.9 Final Discussion of Practical Challenges; 2.2.10 Conclusions on the Transfer of Methods from HPLC to UHPLC 2.3 TRANSFERRING UHPLC METHODS TO HPLC PLATFORMS2.3.1 UHPLC to Traditional HPLC Columns; 2.3.2 UHPLC to Superficially Porous Particle HPLC Columns; 2.3.3 UHPLC to HPLC Method Transfer; 2.4 TRANSFERRING LC METHODS TO OTHER LABS; 2.4.1 UHPLC Instrument Issues Encountered During Method Transfers; 2.4.2 Back to LC Basics; REFERENCES; Chapter 3 Practical Aspects of Ultrahigh Performance Liquid Chromatography; 3.1 INTRODUCTION; 3.2 EFFECT OF EXTRA-COLUMN VOLUME ON PERFORMANCE OF SUB-2 lM PARTICLE-PACKED COLUMNS IN UHPLC; 3.2.1 Theoretical Considerations 3.2.2 Extra-Column Volumes and Column Volumes3.2.3 Effect of Extra-Column Volume on Performance; 3.3 COLUMN PRESSURE AND FRICTIONAL HEATING; 3.3.1 Column Pressure vs. Particle Size; 3.3.2 Phenomenon of Frictional Heating; 3.3.3 Effect of Frictional Heating on Retention Factor; 3.3.4 Factors Leading to Frictional Heating; 3.3.5 How to Resolve Thermal Gradient Issues; 3.3.6 Effect of Pressure on Mobile Phase Characteristics; 3.3.7 Effect of Pressure on Retention Factor with Minimal Frictional Heating; 3.4 METHOD TRANSFER BETWEEN UHPLC AND HPLC AND OTHER TIPS 3.4.1 Column Dimension and Particle Size |
Record Nr. | UNINA-9910139018103321 |
Hoboken, N.J., : John Wiley & Sons Inc., 2013 | ||
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Lo trovi qui: Univ. Federico II | ||
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Ultra-high performance liquid chromatography and its applications [[electronic resource] /] / edited by Quanyun Alan Xu |
Edizione | [1st ed.] |
Pubbl/distr/stampa | Hoboken, N.J., : John Wiley & Sons Inc., 2013 |
Descrizione fisica | 1 online resource (306 p.) |
Disciplina | 543/.84 |
Altri autori (Persone) | XuQuanyun A. <1961-> |
Soggetto topico | Liquid chromatography |
ISBN |
1-118-53398-4
1-118-53395-X 1-299-46494-7 1-118-53399-2 |
Classificazione | TEC009010 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Ultra-High Performance Liquid Chromatography and Its Applications; Contents; Preface; Contributors; Chapter 1 UHPLC Method Development; 1.1 INTRODUCTION; 1.2 METHOD DEVELOPMENT; 1.2.1 Gather Sample Information and Define Method Goals; 1.2.2 Scout Columns and Mobile Phases; 1.2.3 Analyze Scouting Results and Select Separation Conditions; 1.2.4 Optimize the Method; 1.2.5 Validate the Method; 1.2.6 Phase-Appropriate Method Development; 1.2.7 Very High Pressure and Frictional Heating in UHPLC; 1.2.8 Relevance of Various Instrumentation to Method Development
1.2.9 Method Resolution and Speed Requirements1.2.10 Example 1.1; 1.2.11 Example 1.2; 1.3 CONVERSION OF AN HPLC METHOD TO UHPLC; 1.3.1 Select a UHPLC Column with the Same Chemistry; 1.3.2 Measure Dwell Volumes of HPLC and UHPLC Systems; 1.3.3 Calculate Injection Volume, Flow Rate, Holding Time, and Gradient Segment Time; 1.3.4 Perform Verification Experiment; 1.3.5 Validate the Method; 1.3.6 Example 1.3; 1.3.7 Example 1.4; 1.4 CONVERSION OF A UHPLC METHOD TO HPLC; 1.5 SUMMARY; REFERENCES; Chapter 2 Method Transfer Between HPLC and UHPLC Platforms; 2.1 INTRODUCTION 2.2 TRANSFERRING HPLC METHODS TO UHPLC2.2.1 Fundamental Physical Relationships; 2.2.2 Types of UHPLC Applications; 2.2.3 Additional Fundamental Considerations; 2.2.4 Transfer of Isocratic Methods from HPLC to UHPLC; 2.2.5 Cautions on Transferring Isocratic Methods from HPLC to UHPLC; 2.2.6 Transfer of Gradient Methods from HPLC to UHPLC; 2.2.7 Cautions on the Scaling of Gradient Methods from HPLC to UHPLC; 2.2.8 Case Studies and Recommendations; 2.2.9 Final Discussion of Practical Challenges; 2.2.10 Conclusions on the Transfer of Methods from HPLC to UHPLC 2.3 TRANSFERRING UHPLC METHODS TO HPLC PLATFORMS2.3.1 UHPLC to Traditional HPLC Columns; 2.3.2 UHPLC to Superficially Porous Particle HPLC Columns; 2.3.3 UHPLC to HPLC Method Transfer; 2.4 TRANSFERRING LC METHODS TO OTHER LABS; 2.4.1 UHPLC Instrument Issues Encountered During Method Transfers; 2.4.2 Back to LC Basics; REFERENCES; Chapter 3 Practical Aspects of Ultrahigh Performance Liquid Chromatography; 3.1 INTRODUCTION; 3.2 EFFECT OF EXTRA-COLUMN VOLUME ON PERFORMANCE OF SUB-2 lM PARTICLE-PACKED COLUMNS IN UHPLC; 3.2.1 Theoretical Considerations 3.2.2 Extra-Column Volumes and Column Volumes3.2.3 Effect of Extra-Column Volume on Performance; 3.3 COLUMN PRESSURE AND FRICTIONAL HEATING; 3.3.1 Column Pressure vs. Particle Size; 3.3.2 Phenomenon of Frictional Heating; 3.3.3 Effect of Frictional Heating on Retention Factor; 3.3.4 Factors Leading to Frictional Heating; 3.3.5 How to Resolve Thermal Gradient Issues; 3.3.6 Effect of Pressure on Mobile Phase Characteristics; 3.3.7 Effect of Pressure on Retention Factor with Minimal Frictional Heating; 3.4 METHOD TRANSFER BETWEEN UHPLC AND HPLC AND OTHER TIPS 3.4.1 Column Dimension and Particle Size |
Record Nr. | UNINA-9910822370003321 |
Hoboken, N.J., : John Wiley & Sons Inc., 2013 | ||
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Lo trovi qui: Univ. Federico II | ||
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