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Principles and applications of fluorescence spectroscopy [[electronic resource] /] / Jihad René Albani
| Principles and applications of fluorescence spectroscopy [[electronic resource] /] / Jihad René Albani |
| Autore | Albani Jihad René <1956-> |
| Pubbl/distr/stampa | Oxford ; ; Ames, Iowa, : Blackwell Science, c2007 |
| Descrizione fisica | 1 online resource (270 p.) |
| Disciplina | 543/.56 |
| Soggetto topico | Fluorescence spectroscopy |
| ISBN |
1-281-31900-7
9786611319007 0-470-69205-7 0-470-69133-6 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Principles and Applications of Fluorescence Spectroscopy; Contents; 1 Absorption Spectroscopy Theory; 1.1 Introduction; 1.2 Characteristics of an Absorption Spectrum; 1.3 Beer-Lambert-Bouguer Law; 1.4 Effect of the Environment on Absorption Spectra; References; 2 Determination of the Calcofluor White Molar Extinction Coefficient Value in the Absence and Presence of a1-Acid Glycoprotein; 2.1 Introduction; 2.2 Biological Material Used; 2.2.1 Calcofluor White; 2.2.2 a1-Acid glycoprotein; 2.3 Experiments; 2.3.1 Absorption spectrum of Calcofluor free in PBS buffer
2.3.2 Determination of e. value of Calcofluor White free in PBS buffer 2.3.3 Determination of Calcofluor White e. value in the presence of a1-acid glycoprotein; 2.4 Solution; References; 3 Determination of Kinetic Parameters of Lactate Dehydrogenase; 3.1 Objective of the Experiment; 3.2 Absorption Spectrum of NADH; 3.3 Absorption Spectrum of LDH; 3.4 Enzymatic Activity of LDH; 3.5 Kinetic Parameters; 3.6 Data and Results; 3.6.1 Determination of enzyme activity; 3.6.2 Determination of kinetic parameters; 3.7 Introduction to Kinetics and the Michaelis-Menten Equation; 3.7.1 Definitions 3.7.2 Reaction rates References; 4 Hydrolysis of p-Nitrophenyl-B-D-Galactoside with B-Galactosidase from E. coli; 4.1 Introduction; 4.2 Solutions to be Prepared; 4.3 First-day Experiments; 4.3.1 Absorption spectrum of PNP; 4.3.2 Absorption of PNP as a function of pH; 4.3.3 Internal calibration of PNP; 4.3.4 Determination of B-galactosidase optimal pH; 4.3.5 Determination of B-galactosidase optimal temperature; 4.4 Second-day Experiments; 4.4.1 Kinetics of p-nitrophenyl-B-D-galactoside hydrolysis with B-galactosidase; 4.4.2 Determination of the B-galactosidase concentration in the test tube 4.5 Third-day Experiments 4.5.1 Determination of Km and Vmax of B-galactosidase; 4.5.2 Inhibiton of hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; 4.6 Fourth-day Experiments; 4.6.1 Effect of guanidine chloride concentration on B-galactosidase activity; 4.6.2 OD variation with guanidine chloride; 4.6.3 Mathematical derivation of Keq; 4.6.4 Definition of the standard Gibbs free energy, G.; 4.6.5 Relation between G. and G; 4.6.6 Relation between G. and Keq; 4.6.7 Effect of guanidine chloride on hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; References 5 Starch Hydrolysis by Amylase 5.1 Objectives; 5.2 Introduction; 5.3 Materials; 5.4 Procedures and Experiments; 5.4.1 Preparation of a 20 g lŠ1starch solution; 5.4.2 Calibration curve for starch concentration; 5.4.3 Calibration curve for sugar concentration; 5.4.4 Effect of pH; 5.4.5 Temperature effect; 5.4.6 Effect of heat treatment at 90.C; 5.4.7 Kinetics of starch hydrolysis; 5.4.8 Effect of inhibitor (CuCl2) on the amylase activity; 5.4.9 Effect of amylase concentration; 5.4.10 Complement experiments that can be performed; 5.4.11 Notes; References; 6 Determination of the pK of a Dye 6.1 Definition of pK |
| Record Nr. | UNINA-9910145261503321 |
Albani Jihad René <1956->
|
||
| Oxford ; ; Ames, Iowa, : Blackwell Science, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Principles and applications of fluorescence spectroscopy [[electronic resource] /] / Jihad René Albani
| Principles and applications of fluorescence spectroscopy [[electronic resource] /] / Jihad René Albani |
| Autore | Albani Jihad René <1956-> |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Oxford ; ; Ames, Iowa, : Blackwell Science, c2007 |
| Descrizione fisica | 1 online resource (270 p.) |
| Disciplina | 543/.56 |
| Soggetto topico | Fluorescence spectroscopy |
| ISBN |
1-281-31900-7
9786611319007 0-470-69205-7 0-470-69133-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Principles and Applications of Fluorescence Spectroscopy; Contents; 1 Absorption Spectroscopy Theory; 1.1 Introduction; 1.2 Characteristics of an Absorption Spectrum; 1.3 Beer-Lambert-Bouguer Law; 1.4 Effect of the Environment on Absorption Spectra; References; 2 Determination of the Calcofluor White Molar Extinction Coefficient Value in the Absence and Presence of a1-Acid Glycoprotein; 2.1 Introduction; 2.2 Biological Material Used; 2.2.1 Calcofluor White; 2.2.2 a1-Acid glycoprotein; 2.3 Experiments; 2.3.1 Absorption spectrum of Calcofluor free in PBS buffer
2.3.2 Determination of e. value of Calcofluor White free in PBS buffer 2.3.3 Determination of Calcofluor White e. value in the presence of a1-acid glycoprotein; 2.4 Solution; References; 3 Determination of Kinetic Parameters of Lactate Dehydrogenase; 3.1 Objective of the Experiment; 3.2 Absorption Spectrum of NADH; 3.3 Absorption Spectrum of LDH; 3.4 Enzymatic Activity of LDH; 3.5 Kinetic Parameters; 3.6 Data and Results; 3.6.1 Determination of enzyme activity; 3.6.2 Determination of kinetic parameters; 3.7 Introduction to Kinetics and the Michaelis-Menten Equation; 3.7.1 Definitions 3.7.2 Reaction rates References; 4 Hydrolysis of p-Nitrophenyl-B-D-Galactoside with B-Galactosidase from E. coli; 4.1 Introduction; 4.2 Solutions to be Prepared; 4.3 First-day Experiments; 4.3.1 Absorption spectrum of PNP; 4.3.2 Absorption of PNP as a function of pH; 4.3.3 Internal calibration of PNP; 4.3.4 Determination of B-galactosidase optimal pH; 4.3.5 Determination of B-galactosidase optimal temperature; 4.4 Second-day Experiments; 4.4.1 Kinetics of p-nitrophenyl-B-D-galactoside hydrolysis with B-galactosidase; 4.4.2 Determination of the B-galactosidase concentration in the test tube 4.5 Third-day Experiments 4.5.1 Determination of Km and Vmax of B-galactosidase; 4.5.2 Inhibiton of hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; 4.6 Fourth-day Experiments; 4.6.1 Effect of guanidine chloride concentration on B-galactosidase activity; 4.6.2 OD variation with guanidine chloride; 4.6.3 Mathematical derivation of Keq; 4.6.4 Definition of the standard Gibbs free energy, G.; 4.6.5 Relation between G. and G; 4.6.6 Relation between G. and Keq; 4.6.7 Effect of guanidine chloride on hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; References 5 Starch Hydrolysis by Amylase 5.1 Objectives; 5.2 Introduction; 5.3 Materials; 5.4 Procedures and Experiments; 5.4.1 Preparation of a 20 g lŠ1starch solution; 5.4.2 Calibration curve for starch concentration; 5.4.3 Calibration curve for sugar concentration; 5.4.4 Effect of pH; 5.4.5 Temperature effect; 5.4.6 Effect of heat treatment at 90.C; 5.4.7 Kinetics of starch hydrolysis; 5.4.8 Effect of inhibitor (CuCl2) on the amylase activity; 5.4.9 Effect of amylase concentration; 5.4.10 Complement experiments that can be performed; 5.4.11 Notes; References; 6 Determination of the pK of a Dye 6.1 Definition of pK |
| Record Nr. | UNISA-996218619003316 |
|
Albani Jihad René <1956->
|
||
| Oxford ; ; Ames, Iowa, : Blackwell Science, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
Principles and applications of fluorescence spectroscopy [[electronic resource] /] / Jihad René Albani
| Principles and applications of fluorescence spectroscopy [[electronic resource] /] / Jihad René Albani |
| Autore | Albani Jihad René <1956-> |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Oxford ; ; Ames, Iowa, : Blackwell Science, c2007 |
| Descrizione fisica | 1 online resource (270 p.) |
| Disciplina | 543/.56 |
| Soggetto topico | Fluorescence spectroscopy |
| ISBN |
1-281-31900-7
9786611319007 0-470-69205-7 0-470-69133-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Principles and Applications of Fluorescence Spectroscopy; Contents; 1 Absorption Spectroscopy Theory; 1.1 Introduction; 1.2 Characteristics of an Absorption Spectrum; 1.3 Beer-Lambert-Bouguer Law; 1.4 Effect of the Environment on Absorption Spectra; References; 2 Determination of the Calcofluor White Molar Extinction Coefficient Value in the Absence and Presence of a1-Acid Glycoprotein; 2.1 Introduction; 2.2 Biological Material Used; 2.2.1 Calcofluor White; 2.2.2 a1-Acid glycoprotein; 2.3 Experiments; 2.3.1 Absorption spectrum of Calcofluor free in PBS buffer
2.3.2 Determination of e. value of Calcofluor White free in PBS buffer 2.3.3 Determination of Calcofluor White e. value in the presence of a1-acid glycoprotein; 2.4 Solution; References; 3 Determination of Kinetic Parameters of Lactate Dehydrogenase; 3.1 Objective of the Experiment; 3.2 Absorption Spectrum of NADH; 3.3 Absorption Spectrum of LDH; 3.4 Enzymatic Activity of LDH; 3.5 Kinetic Parameters; 3.6 Data and Results; 3.6.1 Determination of enzyme activity; 3.6.2 Determination of kinetic parameters; 3.7 Introduction to Kinetics and the Michaelis-Menten Equation; 3.7.1 Definitions 3.7.2 Reaction rates References; 4 Hydrolysis of p-Nitrophenyl-B-D-Galactoside with B-Galactosidase from E. coli; 4.1 Introduction; 4.2 Solutions to be Prepared; 4.3 First-day Experiments; 4.3.1 Absorption spectrum of PNP; 4.3.2 Absorption of PNP as a function of pH; 4.3.3 Internal calibration of PNP; 4.3.4 Determination of B-galactosidase optimal pH; 4.3.5 Determination of B-galactosidase optimal temperature; 4.4 Second-day Experiments; 4.4.1 Kinetics of p-nitrophenyl-B-D-galactoside hydrolysis with B-galactosidase; 4.4.2 Determination of the B-galactosidase concentration in the test tube 4.5 Third-day Experiments 4.5.1 Determination of Km and Vmax of B-galactosidase; 4.5.2 Inhibiton of hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; 4.6 Fourth-day Experiments; 4.6.1 Effect of guanidine chloride concentration on B-galactosidase activity; 4.6.2 OD variation with guanidine chloride; 4.6.3 Mathematical derivation of Keq; 4.6.4 Definition of the standard Gibbs free energy, G.; 4.6.5 Relation between G. and G; 4.6.6 Relation between G. and Keq; 4.6.7 Effect of guanidine chloride on hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; References 5 Starch Hydrolysis by Amylase 5.1 Objectives; 5.2 Introduction; 5.3 Materials; 5.4 Procedures and Experiments; 5.4.1 Preparation of a 20 g lŠ1starch solution; 5.4.2 Calibration curve for starch concentration; 5.4.3 Calibration curve for sugar concentration; 5.4.4 Effect of pH; 5.4.5 Temperature effect; 5.4.6 Effect of heat treatment at 90.C; 5.4.7 Kinetics of starch hydrolysis; 5.4.8 Effect of inhibitor (CuCl2) on the amylase activity; 5.4.9 Effect of amylase concentration; 5.4.10 Complement experiments that can be performed; 5.4.11 Notes; References; 6 Determination of the pK of a Dye 6.1 Definition of pK |
| Record Nr. | UNINA-9910824044703321 |
|
Albani Jihad René <1956->
|
||
| Oxford ; ; Ames, Iowa, : Blackwell Science, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Single molecule detection in solution : methods and applications
| Single molecule detection in solution : methods and applications |
| Pubbl/distr/stampa | [Place of publication not identified], : Wiley VCH, 2002 |
| Descrizione fisica | 1 online resource (377 pages) |
| Disciplina | 543/.56 |
| Soggetto topico |
Fluorescence spectroscopy
Raman spectroscopy Molecules Solution (Chemistry) Analytical Chemistry Chemistry Physical Sciences & Mathematics |
| ISBN |
1-280-55847-4
9786610558476 3-527-60080-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910146233103321 |
| [Place of publication not identified], : Wiley VCH, 2002 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Single molecule detection in solution : methods and applications
| Single molecule detection in solution : methods and applications |
| Pubbl/distr/stampa | [Place of publication not identified], : Wiley VCH, 2002 |
| Descrizione fisica | 1 online resource (377 pages) |
| Disciplina | 543/.56 |
| Soggetto topico |
Fluorescence spectroscopy
Raman spectroscopy Molecules Solution (Chemistry) Analytical Chemistry Chemistry Physical Sciences & Mathematics |
| ISBN |
1-280-55847-4
9786610558476 3-527-60080-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910830150003321 |
| [Place of publication not identified], : Wiley VCH, 2002 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Single molecule detection in solution : methods and applications
| Single molecule detection in solution : methods and applications |
| Pubbl/distr/stampa | [Place of publication not identified], : Wiley VCH, 2002 |
| Descrizione fisica | 1 online resource (377 pages) |
| Disciplina | 543/.56 |
| Soggetto topico |
Fluorescence spectroscopy
Raman spectroscopy Molecules Solution (Chemistry) Analytical Chemistry Chemistry Physical Sciences & Mathematics |
| ISBN |
1-280-55847-4
9786610558476 3-527-60080-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910841603703321 |
| [Place of publication not identified], : Wiley VCH, 2002 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Standard practice for determining the relative spectral correction factors for the emission signal of fluorescence spectrometers / / Paul C. DeRose
| Standard practice for determining the relative spectral correction factors for the emission signal of fluorescence spectrometers / / Paul C. DeRose |
| Autore | DeRose Paul C |
| Pubbl/distr/stampa | Gaithersburg, MD : , : U.S. Dept. of Commerce, National Institute of Standards and Technology, , 2013 |
| Descrizione fisica | 1 online resource (14 pages) : illustrations (some color) |
| Altri autori (Persone) | DeRosePaul C |
| Collana | NISTIR |
| Soggetto topico |
Fluorescence spectroscopy
Fluorescence |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910710515103321 |
|
DeRose Paul C
|
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| Gaithersburg, MD : , : U.S. Dept. of Commerce, National Institute of Standards and Technology, , 2013 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Surface plasmon enhanced, coupled, and controlled fluorescence / / edited by Chris D Geddes
| Surface plasmon enhanced, coupled, and controlled fluorescence / / edited by Chris D Geddes |
| Pubbl/distr/stampa | Hoboken, New Jersey : , : Wiley, , 2017 |
| Descrizione fisica | 1 online resource (342 pages) : illustrations, tables, photographs |
| Disciplina | 535/.352 |
| Soggetto topico |
Fluorescence
Fluorescence spectroscopy Surface plasmon resonance Plasmons (Physics) |
| ISBN |
1-119-32482-3
1-119-32516-1 1-119-32589-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910270928203321 |
| Hoboken, New Jersey : , : Wiley, , 2017 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Surface plasmon enhanced, coupled, and controlled fluorescence / / edited by Chris D Geddes
| Surface plasmon enhanced, coupled, and controlled fluorescence / / edited by Chris D Geddes |
| Pubbl/distr/stampa | Hoboken, New Jersey : , : Wiley, , 2017 |
| Descrizione fisica | 1 online resource (342 pages) : illustrations, tables, photographs |
| Disciplina | 535/.352 |
| Soggetto topico |
Fluorescence
Fluorescence spectroscopy Surface plasmon resonance Plasmons (Physics) |
| ISBN |
1-119-32482-3
1-119-32516-1 1-119-32589-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910828418703321 |
| Hoboken, New Jersey : , : Wiley, , 2017 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Topics in Fluorescence Spectroscopy : Principles [[electronic resource] /] / edited by Joseph R. Lakowicz
| Topics in Fluorescence Spectroscopy : Principles [[electronic resource] /] / edited by Joseph R. Lakowicz |
| Edizione | [1st ed. 2002.] |
| Pubbl/distr/stampa | New York, : Kluwer Academic, c2002 |
| Descrizione fisica | 1 online resource (449 p.) |
| Disciplina | 543/.0858 |
| Altri autori (Persone) | LakowiczJoseph R |
| Collana | Topics in fluorescence spectroscopy |
| Soggetto topico |
Diagnostic imaging
Fluorescence spectroscopy Lasers - Diagnostic use |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-280-20633-0
9786610206339 0-306-47058-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Fluorescence Anisotropy: Theory and Applications -- Fluorescence Quenching: Theory and Applications -- Resonance Energy Transfer -- Least-Squares Analysis of Fluorescence Data -- The Global Analysis of Fluorescence Intensity and Anisotropy Decay Data: Second-Generation Theory and Programs -- Fluorescence Polarization from Oriented Systems -- Fluorescence-Based Fiber-Optic Sensors -- Inhomogeneous Broadening of Electronic Spectra of Dye Molecules in Solutions. |
| Record Nr. | UNINA-9910454981803321 |
| New York, : Kluwer Academic, c2002 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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