Pubbl/distr/stampa	Norfolk, England : , : Caister Academic Press, , [2015]
Descrizione fisica	1 online resource (231 p.)
Disciplina	574.87028
Soggetto topico	Flow cytometry Microbiology - Technique
Soggetto genere / forma	Electronic books.
ISBN	1-910190-12-8
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Nota di contenuto	Contents; Current Books of Interest; Contributors; Introduction; 1: Microbial Cytometry: What It Was, Is, and May Be; Introduction; Tasks in microbial cytometry: motives and methods; From microscopy to cytometry: more than meets the eye; Intrinsic parameters; Extrinsic parameters; Microscopy versus cytometry: the eyes do not have it; Cytometric options: thinking outside and inside the box; 2: Non-destructive On-chip Imaging Cytometry Assay for Constructive On-chip Cellomics Studies; Introduction; A fully automated on-chip imaging flow cytometry system Applications of on-chip imaging cytometry technologiesSummary; 3: Application of Flow Cytometry to Environmental Biotechnology; Introduction; Application to drinking water quality; Application to wastewater treatments; Application to solid waste treatments; Bacterial viability in soils and sediments; Future trends; 4: Flow Cytometry and Microbial Community Fingerprinting; What are natural communities?; Community structure analysis using flow cytometry; Community composition analysis using fingerprinting techniques Combination of community fingerprinting and single cell analysis by cell sorting5: Application of Flow Cytometry to the Detection of Pathogenic Bacteria; Microbial pathogenesis; Advantages of flow cytometry for pathogen detection; Challenges in pathogen detection; Instrument set up; Sample preparation; Immunolabelling; Fluorescence in situ hybridisation (FISH); Green fluorescent protein (GFP); Quantum dot labelling; FCM analysis of food samples; FCM analysis of clinical samples; Physiology of microbial pathogens and FCM analysis; Cell separation/cell sorting; Conclusion 6: The Use of Flow Cytometry to Study Sporeforming BacteriaIntroduction; The importance of spore formers; Spore formers and flow cytometry: a review of past studies; Potential applications of flow cytometry to the study of spore formers; Conclusions; 7: Flow Cytometry of Yeasts and Other Fungi; Introduction; Flow cytometry and FACS to investigate cell and molecular biology; Medical applications of FCM; FCM for bioprocess monitoring and development; Cell engineering and screening using FACS; Molecular screening methods using yeast and FACS New methods and technology related to, and developed from, FCM and FACSConclusion; 8: The Application of Flow Cytometry to the Study of Lactic Acid Bacteria Fermentations; Introduction; Traditional methods used to monitor LAB and a comparison with flow cytometry methodology; Flow cytometry (FCM); Specific examples of flow cytometry and LAB analysis; Starter performance; Cheese manufacture and FCM applications; Probiotic bacteria; Sample preparation; Conclusion; 9: Flow Cytometry for Rapid Microbiological Analysis of Drinking Water: From Science to Practice - An Unfinished Story Setting the scene
Record Nr.	UNINA-9910461020803321

Autore	Ortolani C (Claudio)
Edizione	[Second edition.]
Pubbl/distr/stampa	Hoboken, New Jersey ; ; Chichester, West Sussex, England : , : Wiley Blackwell, , [2021]
Descrizione fisica	1 online resource (467 pages)
Disciplina	574.87028
Soggetto topico	Flow cytometry Hematological oncology Flow cytometry - Methodology
Soggetto genere / forma	Electronic books.
ISBN	1-119-61130-X 1-119-61127-X 1-119-61128-8
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Nota di contenuto	Cover -- Title Page -- Copyright Page -- Contents -- Foreword to the Second Edition -- Foreword to the First Edition -- Foreword to the First Edition -- Preface to the Second Edition -- Preface to the First Edition -- Abbreviations -- Chapter 1 Antigens -- CD1 Antigens -- CD2 Antigen -- CD3 Antigen -- CD4 Antigen -- CD5 Antigen -- CD7 Antigen -- CD8 Antigen -- CD10 Antigen -- CD11b Antigen -- CD11c Antigen -- CD13 Antigen -- CD14 Antigen -- CD15 Antigen -- CD16 Antigen -- CD19 Antigen -- CD20 Antigen -- CD22 Antigen -- CD23 Antigen -- CD24 Antigen -- CD25 Antigen -- CD26 Antigen -- CD27 Antigen -- CD28 Antigen -- CD30 Antigen -- CD33 Antigen -- CD34 Antigen -- CD38 Antigen -- CD43 Antigen -- CD45 Antigen -- CD45 Isoforms -- CD49 Antigens -- CD56 Antigen -- CD57 Antigen -- CD61 Antigen -- CD62L Antigen -- CD64 Antigen -- CD65 Antigen -- CD66c Antigen -- CD71 Antigen -- CD79 Antigen -- CD81 Antigen -- CD103 Antigen -- CD117 Antigen -- CD123 Antigen -- CD138 Antigen -- CD200 Antigen -- CD305 Antigen -- CD307 (IRTA) Family -- CD371 Antigen -- BCL-2 Protein -- Chemokines and Chemokine Receptors -- Chemokines -- Chemokine Receptors -- CRLF2 Antigen -- Cytotoxic Proteins -- HLA-DR Antigen -- Immunoglobulins -- KIRs, CD158 Isoforms -- Myeloperoxidase -- NG2 Antigen -- PCA-1 Antigen -- ROR1 Antigen -- SLAM Molecules and SLAM-Associated Protein -- SOX11 -- T-Cell Receptor (TCR) -- Terminal Deoxy-Nucleotidyl Transferase -- Toll-Like Receptors -- VS38 Antigen -- ZAP-70 Protein -- Chapter 2 Diseases -- Myeloproliferative neoplasms -- Chronic myeloid leukemia (CML) -- Myeloproliferative neoplasms other than CML -- Chronic neutrophilic leukemia (CNL) -- Polycythemia vera (PV) -- Primary myelofibrosis (PMF) -- Essential thrombocythemia (ET) -- Chronic eosinophilic leukemia (CEL) -- Mastocytosis -- Acute mast-cell leukemia (AMCL). Chronic mast-cell leukemia (CMCL) -- Myelomastocytic leukemia (MML) -- Myelodysplastic/myeloproliferative neoplasms -- Chronic myelomonocytic leukemia (CMML) -- Other myelodysplastic/myeloproliferative neoplasms and related conditions -- Juvenile myelomonocytic leukemia (JMML) -- Atypical CML bcr/abl negative (ACML) -- RAS-associated autoimmune leukoproliferative disorder (RALD) -- Myelodysplastic syndromes -- Myeloid neoplasms with germline predisposition -- Acute myeloid leukemias -- AMLs with recurrent genetic anomalies -- AMLs with chromosomal anomalies -- AMLs with gene mutations -- Relationships between genotype and phenotype in cases of AML not recognized as separate entities in WHO 2017 -- AMLs with myelodysplasia-related changes (AML-MRC) -- AMLs not otherwise specified -- AML with minimal differentiation -- AML without maturation -- AML with maturation -- Acute myelomonocytic leukemia (AMMoL) -- Acute monoblastic and monocytic leukemia (AMoL) -- Pure erythroid leukemia (PEL) -- Acute megakaryoblastic leukemia (AMKL) -- Acute basophilic leukemia (ABL) -- Myeloid proliferations associated with Down syndrome -- Transient abnormal myelopoiesis (TAM) -- AMLs in patients with Down syndrome -- Blastic plasmacytoid dendritic cell neoplasm (BPDCN/PDCL) -- Acute leukemias with ambiguous lineage attribution (ALAL) -- Acute undifferentiated leukemias (AUL) -- Mixed phenotype acute leukemias (MPAL) -- Neoplastic diseases of B and T lymphatic precursors -- B lymphoblastic leukemia/lymphoma, not otherwise specified (B-ALL/LBLnos) -- B lymphoblastic leukemia/lymphoma with recurrent genetic anomalies -- Relationships between genotype and phenotype in cases of B-ALL not recognized as separate entities in WHO 2017 -- T lymphoblastic leukemia/lymphoma (T-ALL/LBL) -- Early T-cell precursor lymphoblastic leukemia (ETP-ALL). NK lymphoblastic leukemia/lymphoma (NK-ALL/LBL) -- Neoplastic diseases of mature B cells -- Chronic lymphocytic leukemia/small lymphocytic lymphoma (B-CLL/SLL) -- Familial B-CLL -- Richter syndrome -- Monoclonal B-cell lymphocytosis (MBL) -- CLL-like monoclonal B lymphocytosis -- Non-CLL-like monoclonal B lymphocytosis -- B-cell prolymphocytic leukemia (B-PLL) -- Lymphoplasmacytic lymphoma (LPL) -- Heavy chain disease (HCD) -- μ heavy chain disease -- α heavy chain disease -- Hairy cell leukemia (HCL) -- Hairy cell leukemia, variant (HCL-v) -- Hairy cell leukemia, Japanese variant (HCL-J) -- Splenic diffuse red pulp lymphoma (SDRPL) -- Marginal zone lymphomas (MZL) -- Nodal marginal zone lymphoma (NMZL) -- Splenic marginal zone lymphoma (SMZL) -- Extranodal marginal zone lymphoma (EMZL/MALToma) -- Clonal B-cell lymphocytosis with MZL-like phenotype (CBL-MZ) -- Follicular lymphoma (FCL) -- Testicular follicular lymphoma -- Duodenal type follicular lymphoma -- Pediatric type follicular lymphoma -- Primitive cutaneous follicular lymphoma (PCFL) -- Large B-cell lymphoma with IRF4 rearrangement -- Mantle-cell lymphoma (MCL) -- Blastic mantle-cell lymphoma (BMCL) -- Leukemic non nodal mantle-cell lymphoma -- DLBCL not otherwise specified (DLBCLnos) -- CD5(+) diffuse large cell lymphoma (CD5(+) DLBCL) -- T-cell/histiocyte-rich B-cell lymphoma (THRLBCL) -- Primary DLBCL of the CNS (PCNSL) -- Primary cutaneous DLBCL, "leg type -- EBV(+) DLBCLnos -- DLBCL associated with chronic inflammation (PAL) -- Fibrin associated DLBCL -- Lymphomatoid granulomatosis (LYG) -- Primary mediastinal B-cell lymphoma (PMBCL) -- Intravascular large B-cell lymphoma (IVBCL) -- ALK-positive large cell lymphoma (ALK(+) LBCL) -- Plasmablastic lymphoma (PBL) -- Primary effusion lymphoma (PEL) -- HHV8-associated lymphoproliferative disorders -- HHV8-positive DLBCL. HHV8-positive germinotropic lymphoproliferative disorder -- Burkitt lymphoma (BL) -- Burkitt leukemia with immature phenotype -- Burkitt-like lymphoma with 11q aberrations -- High-grade B-cell lymphoma (HGBL) -- Plasma cell neoplasms -- Monoclonal gammopathies of undetermined significance (MGUS) -- Multiple myeloma (MM) -- Plasma cell leukemia (PCL) -- Neoplastic diseases of mature T and NK cells -- T-cell prolymphocytic leukemia (T-PLL) -- T-cell large granular lymphocytic leukemia (T-LGL) -- Chronic lymphoproliferative disorders of NK cells (CLPD-NK/CNKL) -- Aggressive NK-cell leukemia (ANKL) -- Adult T-cell leukemia/lymphoma (ATLL) -- Extranodal NK/T-cell lymphoma, "nasal type" (ENKTL) -- Intestinal T-cell lymphomas (ITCL) -- Enteropathy-associated T-cell lymphoma (EATCL) -- Monomorphic epitheliotropic intestinal T-cell lymphoma (MEITL) -- Indolent gastro-intestinal T lymphoproliferative disorder (indolent GI T-LPD) -- Hepatosplenic T-cell lymphoma (HSTCL) -- Subcutaneous panniculitis-like T-cell lymphoma (SPTCL) -- Mycosis fungoides (MF) -- Sezary syndrome (SS) -- Primary cutaneous CD30(+) lymphoproliferative disorders -- Lymphomatoid papulosis (LyP) -- Primary cutaneous anaplastic T-cell lymphoma (pcALCL) -- Primary cutaneous peripheral T-cell lymphoma (PTCL) -- Primary cutaneous TCRƒÁƒÂ(+) T-cell lymphoma (PCGD-TCL) -- Primary cutaneous CD8(+) aggressive epidermotropic cytotoxic T-cell lymphoma (PCAETL) -- Primary cutaneous acral CD8(+) T-cell lymphoma (PCATCL) -- Primary cutaneous lymphoma of the medium/small CD4(+) T cells (PCSM-TCL) -- Peripheral T-cell lymphoma, not otherwise specified (PTCLnos) -- Nodal lymphomas of follicular T-helper derivation -- Angioimmunoblastic T-cell lymphoma (AITL) -- Follicular T-cell lymphoma (FTCL) -- Nodal PTCL with follicular T-helper phenotype -- Anaplastic large cell lymphoma ALK(+) (ALCL ALK(+)). Anaplastic large cell lymphoma ALK(-) (ALCL ALK(-)) -- Breast implant.associated anaplastic large cell lymphoma (biaALCL) -- Hodgkin lymphomas -- Classic Hodgkin lymphoma (CHL) -- Nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) -- Neoplastic diseases of histiocytic and dendritic cells -- Histiocytic sarcoma (HS) -- Langerhans cell histiocytosis (LCH) -- Indeterminate dendritic cell tumor (IDCT) -- Interdigitating dendritic cell sarcoma (IDCS) -- Follicular dendritic cell sarcoma (FDCS) -- Erdheim.Chester disease (EDC) -- Chapter 3 Appendix -- Acute leukemias not recognized by the 2017 WHO classification -- Acute leukemia of myeloid/NK precursors (M/NK-AL) -- Acute leukemia of myeloid dendritic cells (MDCL) -- Acute leukemia of Langerhans cells -- Composite lymphomas -- Hypereosinophilic syndrome (HES), lymphocyte variant -- Indolent T lymphoblastic proliferations (iT]LBP) -- Polyclonal lymphocytoses of B lymphocytes -- Persistent polyclonal B]cell lymphocytosis (PPBL) -- Persistent polyclonal CD5(+) B]cell lymphocytosis -- Persistent polyclonal B]cell lymphocytosis, Japanese (hairy) variant -- Polyclonal plasmacytoses -- Small round (blue) cell tumors (SR(B)CT) -- References -- Index -- EULA.
Record Nr.	UNINA-9910555117403321

Autore	Ortolani Claudio
Pubbl/distr/stampa	Hoboken, : Wiley, 2011
Descrizione fisica	1 online resource (327 p.)
Disciplina	616.99/418 616.99418 616.9941807582
Soggetto topico	Flow cytometry Flow Cytometry - methods Flow Cytometry -- methods Hematologic Neoplasms - diagnosis Hematologic Neoplasms -- diagnosis Hematological oncology Investigative Techniques Neoplasms by Site Cytophotometry Hematologic Diseases Fluorometry Cytological Techniques Neoplasms Hemic and Lymphatic Diseases Diseases Clinical Laboratory Techniques Luminescent Measurements Photometry Chemistry Techniques, Analytical Methods Diagnosis Hematologic Neoplasms Flow Cytometry Medicine Health & Biological Sciences Oncology
ISBN	1-283-17847-8 9786613178473 1-4443-9806-7 1-4443-9804-0
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Nota di contenuto	Flow Cytometry of Hematological Malignancies; Contents; Foreword by Maryalice Stetler-Stevenson; Foreword by Bruno Brando; Preface and acknowledgments; Abbreviations; 1 Antigens; CD1 Antigens; CD2 Antigen; CD3 Antigen; CD4 Antigen; CD5 Antigen; CD7 Antigen; CD8 Antigen; CD10 Antigen; CD11b Antigen; CD11c Antigen; CD13 Antigen; CD14 Antigen; CD15 Antigen; CD16 Antigen; CD19 Antigen; CD20 Antigen; CD22 Antigen; CD23 Antigen; CD24 Antigen; CD25 Antigen; CD30 Antigen; CD33 Antigen; CD34 Antigen; CD38 Antigen; CD45 Antigen; CD45 Isoforms; CD56 Antigen; CD57 Antigen; CD61 Antigen; CD64 Antigen CD65 AntigenCD66c Antigen; CD71 Antigen; CD79 Antigen; CD103 Antigen; CD117 Antigen; CD138 Antigen; HLA-DR Antigen; NG2 Antigen; Immunoglobulins; Myeloperoxidase; Cytotoxic Proteins; KIR, CD158 Isoforms; T Cell Receptor; Terminal Deoxy-nucleotidyl Transferase; Bcl-2 Protein; ZAP-70 Protein; References; 2 Diseases; Myeloproliferative Neoplasms; Myelodysplastic/Myeloproliferative Neoplasms; Myelodysplastic Syndromes; Acute Myeloid Leukemias; Acute Leukemias of Ambigous Lineage Attribution; Acute Leukemias not Recognized by the WHO Classification Neoplastic Diseases of T and B Lymphatic PrecursorsNeoplastic Diseases of Mature B Cells; Neoplastic Diseases of Mature T and NK Cells; Appendix; References; Index; A; B; C; D; E; F; G; H; I; J; K; L; M; N; O; P; R; S; T; U; V; W; X; Z
Record Nr.	UNINA-9910139629903321

Pubbl/distr/stampa	Weinheim, : Wiley-VCH
Descrizione fisica	1 online resource (481 p.)
Disciplina	571.62
Altri autori (Persone)	DoleželJaroslav GreilhuberJohann SudaJan
Soggetto topico	Flow cytometry Plant cells and tissues
Soggetto genere / forma	Electronic books.
ISBN	1-280-92167-6 9786610921676 3-527-61092-8 3-527-61093-6
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Nota di contenuto	Flow Cytometry with Plant Cells; Contents; Preface; List of Contributors; 1 Cytometry and Cytometers: Development and Growth; Overview; 1.1 Origins; 1.2 From Absorption to Fluorescence, from Imaging to Flow; 1.2.1 Early Microspectrophotometry and Image Cytometry; 1.2.2 Fluorescence Microscopy and the Fluorescent Antibody Technique; 1.2.3 Computers Meet Cytometers: The Birth of Analytical Flow Cytometry; 1.2.4 The Development of Cell Sorting; 1.3 The Growth of Multiparameter Flow Cytometry; 1.4 Bench-tops and Behemoths: Convergent Evolution; 1.5 Image Cytometry: New Beginnings?; References 2 Principles of Flow CytometryOverview; 2.1 Introduction; 2.2 A Brief History of Flow Cytometry; 2.3 Components of a Flow Cytometer; 2.3.1 Fluidics; 2.3.2 Optics; 2.3.3 Electronic Systems; 2.4 Flow Cytometric Informatics; 2.5 Spectral Compensation; 2.6 Cell Sorting; 2.7 Calibration Issues; 2.8 Conclusions; References; 3 Flow Cytometry with Plants: an Overview; Overview; 3.1 Introduction; 3.2 Fluorescence is a Fundamental Parameter; 3.3 Pushing Plants through the Flow Cytometer; 3.3.1 Difficulties with Plants and their Cells; 3.3.2 Protoplasts are somewhat ""Easier"" than Intact Cells 3.3.3 Going for Organelles3.4 Application of Flow Cytometry in Plants; 3.4.1 Microspores and Pollen; 3.4.2 Protoplasts; 3.4.2.1 Physiological Processes; 3.4.2.2 Secondary Metabolites; 3.4.2.3 Gene Expression; 3.4.2.4 Somatic Hybrids; 3.4.2.5 DNA Transfection; 3.4.3 Cell Nuclei; 3.4.3.1 Ploidy Levels; 3.4.3.2 Aneuploidy; 3.4.3.3 B Chromosomes; 3.4.3.4 Sex Chromosomes; 3.4.3.5 Cell Cycle and Endopolyploidy; 3.4.3.6 Reproductive Pathways; 3.4.3.7 Nuclear Genome Size; 3.4.3.8 DNA Base Content; 3.4.3.9 Chromatin Composition; 3.4.3.10 Sorting of Nuclei; 3.4.4 Mitotic Chromosomes; 3.4.5 Chloroplasts 3.4.6 Mitochondria3.4.7 Plant Pathogens; 3.4.8 Aquatic Flow Cytometry; 3.5 A Flow Cytometer in Every Laboratory?; 3.6 Conclusions and Future Trends; References; 4 Nuclear DNA Content Measurement; Overview; 4.1 Introduction; 4.2 Nuclear DNA Content: Words, Concepts and Symbols; 4.2.1 Replication-Division Phases; 4.2.2 Alternation of Nuclear Phases; 4.2.3 Generative Polyploidy Levels; 4.2.4 Somatic Polyploidy; 4.3 Units for Presenting DNA Amounts and their Conversion Factors; 4.4 Sample Preparation for Flow Cytometric DNA Measurement; 4.4.1 Selection of the Tissue; 4.4.2 Reagents and Solutions 4.4.2.1 Isolation Buffers and DNA Staining4.5 Standardization; 4.5.1 Types of Standardization; 4.5.2 Requirement of Internal Standardization - a Practical Test; 4.5.3 Choice of the Appropriate Standard Species; 4.5.3.1 Biological Similarity; 4.5.3.2 Genome Size; 4.5.3.3 Nature of the Standard; 4.5.3.4 Availability; 4.5.3.5 Cytological Homogeneity; 4.5.3.6 Accessibility; 4.5.3.7 Reliability of C-Values; 4.5.4 Studies on Plant Standards; 4.5.5 Suggested Standards; 4.6 Fluorescence Inhibitors and Coatings of Debris; 4.6.1 What are Fluorescence Inhibitors and Coatings of Debris? 4.6.2 Experiments with Tannic Acid
Record Nr.	UNINA-9910144559603321

Autore	Ortolani C (Claudio)
Pubbl/distr/stampa	Cham, Switzerland : , : Springer, , [2023]
Descrizione fisica	1 online resource (556 pages)
Disciplina	910.5
Soggetto topico	Flow cytometry
ISBN	9783031108365 9783031108358
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Nota di contenuto	Intro -- Foreword -- Preface -- Contents -- Abbreviations -- Chapter 1: General Principles -- 1.1 Flow Cytometers General Layout -- 1.2 Flow Cytometers Features -- 1.3 Parameters and Signals -- 1.4 Time -- References -- Chapter 2: Signals: Scattering -- 2.1 Forward Scatter (FSC) -- 2.2 Side Scatter (SSC) -- 2.3 Raman Scattering -- 2.4 Depolarized Scatter -- References -- Chapter 3: Signals: Fluorescence, Phosphorescence, Impedance, Extinction -- 3.1 Fluorescence -- 3.1.1 Depolarized Fluorescence -- 3.1.2 Autofluorescence -- 3.2 Phosphorescence -- 3.3 Impedance -- 3.4 Axial Extinction -- References -- Chapter 4: Fluidics -- 4.1 Overview on Fluids -- 4.1.1 Laminar Flow and Turbulent Flow -- 4.1.2 Hydrodynamic Focusing -- 4.2 Cytometer Fluidics -- 4.2.1 Sheath and Core -- 4.2.2 Flow System Components -- 4.2.3 Flow Rate Control -- 4.2.4 Sample Injection -- 4.2.4.1 Sample Differential Control -- 4.2.4.2 Absolute Counts -- 4.2.5 Event-Light Interaction: The Interrogation Point -- 4.2.5.1 In-Cuvette Interaction -- 4.2.5.2 Stream-in-Air Interaction -- 4.2.5.3 Interrogation on an Open Surface -- 4.2.5.4 Systems Based on Acoustic Focusing -- 4.2.5.5 Sheathless Systems -- References -- Chapter 5: Light Sources -- 5.1 Arc Lamps -- 5.2 Lasers -- 5.2.1 Gas Lasers -- 5.2.1.1 Argon Ion Lasers -- 5.2.1.2 Krypton Ion Lasers -- 5.2.1.3 Mixed-Gas Ion Lasers (Argon/Krypton) -- 5.2.1.4 Helium-Neon Atom Lasers -- 5.2.1.5 Helium-Cadmium Ion Lasers -- 5.2.1.6 Helium-Silver and Neon-Copper Metal Vapor Lasers -- 5.2.2 Solid-State Lasers (SSLs) -- 5.2.2.1 Ultraviolet (UV) Emitting SSLs -- Deep UV -- 320 nm -- 355 nm -- 5.2.2.2 Near-Ultraviolet Emitting SSLs -- 5.2.2.3 Violet Emitting SSLs -- 5.2.2.4 Blue Emitting SSLs -- Deep Blue -- Blue-Green -- 5.2.2.5 Green and Yellow-Green Emitting SSLs -- 532 nm -- 552 nm -- 561 nm -- 5.2.2.6 Orange Emitting SSLs. 5.2.2.7 Red Emitting SSLs -- Short Red -- Full Red -- 5.2.2.8 Infrared (IR) Emitting SSLs -- 5.2.2.9 Supercontinuum White Light Emitting SSLs -- 5.2.3 Liquid State Lasers (Dye Lasers) -- 5.3 Light Emitting Diodes (LEDs) -- References -- Chapter 6: Optical Benches -- 6.1 From the Light Source(s) to the Interrogation Point(s) -- 6.2 From the Interrogation Point(s) to the Detector(s) -- 6.3 Optical Bench Components -- 6.3.1 Absorption Filters -- 6.3.2 Interference Filters -- 6.3.3 Neutral Density Filters -- 6.3.4 Polarizing Filters -- 6.3.5 Beam Splitters -- 6.3.6 Wavelength Division Multiplexing (WDM) -- 6.3.7 Prisms, Gratings, Coarse WDM (CWDM) -- 6.4 Optical Bench Layouts -- 6.4.1 Transmission Benches -- 6.4.2 Reflection Benches -- 6.4.3 Multilaser Benches -- 6.4.3.1 Spaced Lasers and Separate Pathways/Detectors -- 6.4.3.2 Spaced Lasers and Shared Pathways/Detectors -- 6.4.3.3 Time Delay -- 6.4.3.4 Continuous Collinear Lasers -- 6.4.3.5 Pulsed Collinear Lasers -- 6.4.4 Special Solutions -- 6.4.4.1 Pie-Shaped Design -- 6.4.4.2 Benches for (De)Polarized Signals -- 6.4.4.3 Spectrally Enhanced Optical Benches -- References -- Chapter 7: Detectors and Electronics -- 7.1 Photodetectors -- 7.1.1 Photodiodes (PDs) -- 7.1.2 Avalanche Photodiodes (APDs) -- 7.1.3 Photomultipliers (PMTs) -- 7.1.4 Multi-anode Photomultipliers -- 7.1.5 Silicon Photomultipliers (SiPMs) -- 7.1.6 Charged-Coupled Devices (CCDs) -- 7.1.7 Trans-impedance Amplifiers (TIAs) -- 7.2 Circuitry -- 7.2.1 Analog Model -- 7.2.1.1 Baseline Restorers -- 7.2.1.2 Comparators and Threshold -- 7.2.1.3 Accessory Circuits -- 7.2.1.4 Amplifiers -- Linear Amplifiers -- Logarithmic Amplifiers -- 7.2.1.5 DC Restorers -- 7.2.1.6 Peak Detectors and Integrators -- Measurement of H (Peak Detectors) -- Measurement of A (Integrators) -- Measurement of W -- Pulse Processing Synchronization. 7.2.1.7 Analog-to-Digital Converters (ADCs) -- Bit Number -- Clock Number -- 7.2.2 Digital Model -- 7.2.2.1 Data Acquisition Boards (DAQs) -- Analog-to-Digital Converters (ADCs) -- Field-Programmable Gate Arrays (FPGAs) -- Digital Signal Processors (DSPs) -- 7.2.2.2 General Considerations on the Digital Model -- 7.2.3 Hybrid Model -- References -- Chapter 8: Signal Analysis -- 8.1 The Background -- 8.1.1 Instrumental Background (BCAL) -- 8.1.2 Experimental Background (Bsos) -- 8.2 The Pulse -- 8.2.1 Pulse Analysis in Analog Systems -- 8.2.2 Pulse Analysis in Digital Systems -- 8.2.2.1 Window´s Gate and Window´s Extension -- 8.2.2.2 Area Scaling -- 8.2.3 Practical Applications of Pulse Analysis -- 8.2.3.1 FSC-A vs. FSC-H vs. FSC-W -- 8.2.3.2 FL-A vs. FL-W -- 8.2.3.3 FL-H vs. FL-W -- 8.3 Dynamic Range of the Signal -- 8.3.1 Effective Resolution -- 8.3.2 Picket Fence Phenomenon -- References -- Chapter 9: The Cytometric File -- 9.1 FCS Format -- 9.2 Segments -- 9.2.1 Header Segment -- 9.2.2 Text Segment -- 9.2.3 Data Segment -- 9.2.4 Analysis Segment -- 9.2.5 Optional Segments -- 9.3 Keywords -- 9.3.1 Standard Keywords -- 9.3.1.1 Required Keywords -- 9.3.1.2 Optional Keywords -- 9.3.2 Non-standard Keywords -- 9.3.3 Relationships Between Keywords and Compensation Procedures -- References -- Chapter 10: Data Transformation -- 10.1 Logarithmic Transformation -- 10.2 Log-Like Transformations -- 10.3 Polynomial Transformation -- References -- Chapter 11: Data Representation -- 11.1 Histogram -- 11.1.1 Histograms of Lin Amplified Data -- 11.1.2 Histograms of Log Amplified/Transformed Data -- 11.1.3 Histograms of Log-Like Transformed Data -- 11.2 Cytograms -- 11.2.1 Representation by Dots (Dot Plot) -- 11.2.2 Representation by Contours (Contour Plot) -- 11.2.2.1 Logarithmic Density -- 11.2.2.2 Probability -- 11.2.3 Representation by False Colors or Gray Tones. 11.2.4 Pseudo-Three-Dimensional Representation -- 11.2.5 Three-Dimensional Representation -- References -- Chapter 12: Data Analysis -- 12.1 Immunofluorescence Measurements -- 12.1.1 The Vexed Question of the Negative Control -- 12.1.1.1 Isotype Control -- 12.1.1.2 Isoclonic Control -- 12.1.1.3 Fluorescence-Minus-One (FMO) Control -- 12.1.1.4 Unstained Control -- 12.1.2 Histograms -- 12.1.2.1 MFI (Mean Fluorescence Intensity) -- 12.1.2.2 RFI (Relative Fluorescence Intensity) -- 12.1.3 Cytograms -- 12.1.4 Weak Positivity in Immunofluorescence -- 12.1.4.1 Weak Positivity with a Negative Component -- 12.1.4.2 Weak Positivity Without an Apparent Negative Component -- 12.2 DNA Content Measurements -- 12.2.1 DNA Content versus BrdU Incorporation -- 12.3 Concept of Gate and Concept of Region -- 12.3.1 Combined (Boolean) Use of Regions and Gates -- 12.3.1.1 In the Determination of Hematopoietic Stem Cells (HSCs) -- ISHAGE Protocol -- 12.3.1.2 In the Determination of the Minimal Residual Disease (MRD) -- 12.3.1.3 In the Augmentation of the Dimensionality in Cell Subset Analysis -- 12.4 Advanced Tools and Future Perspectives -- 12.4.1 Pre-processing Programs -- 12.4.1.1 Quality Assessment -- 12.4.1.2 Data Normalization -- 12.4.1.3 Data Compensation -- 12.4.1.4 Data Transformation -- 12.4.2 Data Processing Programs -- 12.4.2.1 Dimensionality Reduction Based Programs -- 12.4.2.2 Clustering-Based Programs -- References -- Chapter 13: Standards, Setup, Calibration, and Control Techniques -- 13.1 Standards in Flow Cytometry -- 13.1.1 Natural Standards -- 13.1.2 Artificial Standards -- 13.1.2.1 Type 0 Artificial Standards -- 13.1.2.2 Type I Artificial Standards -- 13.1.2.3 Type II Artificial Standards -- Type IIa -- Type IIb -- Type IIc -- 13.1.2.4 Type III Artificial Standards -- Type IIIa -- Type IIIb -- Type IIIc Standards in Daily Practice. 13.1.2.5 Primary Performance Parameters (PPP) -- 13.1.3 Standard Use in Quality Procedures -- 13.1.3.1 Internal Quality Controls (IQCs) -- 13.1.3.2 External Quality Assessments (EQAs) -- 13.2 Optical Bench Setup -- 13.3 Photodetectors´ Setup -- 13.3.1 SDen (Electronic Noise Standard Deviation) -- 13.3.2 PMT Setup -- 13.3.3 APD Setup -- 13.4 Calibration -- 13.4.1 Calibration in ERF -- 13.4.2 Calibration in MESF -- 13.4.3 Calibration in ABC -- 13.4.3.1 With Conjugated Antibodies -- 13.4.3.2 With Unconjugated Antibodies -- 13.4.4 Calibration in FLU -- 13.4.5 Calibration in Nanometers -- 13.5 Instrument Performance and Its Control -- 13.5.1 Linearity -- 13.5.2 Accuracy -- 13.5.2.1 Carry-over -- 13.5.2.2 Count Inaccuracy at High Speed -- 13.5.3 Resolution -- 13.5.4 Sensitivity -- 13.5.4.1 Q, Qr, Stain Index, and Other Indexes -- 13.5.4.2 Antibody Titration -- 13.5.5 Limits of Blank (LOB), Detection (LOD), and Quantification (LOQ) -- 13.5.5.1 LOB, LOD, and LOQ in the Detection of Weak Signals -- LOB (Limit of Blank) -- LOD (Limit of Detection) -- LOQ (Limit of Quantification) -- 13.5.5.2 LOB, LOD, and LOQ in Rare Event Analysis -- LOB (Limit of Blank) -- LOD (Limit of Detection) -- LOQ (Limit of Quantification) -- LOD and LOQ and Minimal Residual Disease (MRD) -- 13.5.6 Precision -- 13.5.7 Specificity -- References -- Chapter 14: Fluorochromes: Overview -- 14.1 Spectral Behavior of Fluorescent Molecules -- 14.2 Relationships with the Environment -- 14.2.1 Spectral Effects -- 14.2.1.1 Bathochromic Effect -- 14.2.1.2 Hypsochromic Effect -- 14.2.1.3 Hyperchromic Effect -- 14.2.1.4 Hypochromic Effect -- 14.2.1.5 Solvatochromic Effect -- 14.2.2 Other Effects -- 14.2.2.1 Extinction or Quenching -- 14.2.2.2 Photodestruction or Photobleaching -- 14.2.2.3 Non-radiative Transfer of Energy (FRET) -- 14.3 Accessory Groups -- References. Chapter 15: Fluorochromes Suitable for Antibody Conjugation.
Record Nr.	UNINA-9910631093503321