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Enzymes involved in glycolysis, fatty acid and amino acid biosynthesis : active site mechanisms and inhibition / / authored by Marco Brito-Arias
| Enzymes involved in glycolysis, fatty acid and amino acid biosynthesis : active site mechanisms and inhibition / / authored by Marco Brito-Arias |
| Autore | Brito Arias Marco Augusto |
| Pubbl/distr/stampa | Singapore : , : Bentham Science Publishers Pte Ltd., , [2020] |
| Descrizione fisica | 1 online resource (334 pages) |
| Disciplina | 572.567 |
| Soggetto topico |
Glycolysis
Amino acids Enzymes - Synthesis |
| Soggetto genere / forma | Electronic books. |
| ISBN | 981-14-6090-6 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910494645403321 |
Brito Arias Marco Augusto
|
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| Singapore : , : Bentham Science Publishers Pte Ltd., , [2020] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Enzymes involved in glycolysis, fatty acid and amino acid biosynthesis : active site mechanisms and inhibition / / authored by Marco Brito-Arias
| Enzymes involved in glycolysis, fatty acid and amino acid biosynthesis : active site mechanisms and inhibition / / authored by Marco Brito-Arias |
| Autore | Brito Arias Marco Augusto |
| Pubbl/distr/stampa | Singapore : , : Bentham Science Publishers Pte Ltd., , [2020] |
| Descrizione fisica | 1 online resource (334 pages) |
| Disciplina | 572.567 |
| Soggetto topico |
Glycolysis
Amino acids Enzymes - Synthesis |
| ISBN | 981-14-6090-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910794337203321 |
|
Brito Arias Marco Augusto
|
||
| Singapore : , : Bentham Science Publishers Pte Ltd., , [2020] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Enzymes involved in glycolysis, fatty acid and amino acid biosynthesis : active site mechanisms and inhibition / / authored by Marco Brito-Arias
| Enzymes involved in glycolysis, fatty acid and amino acid biosynthesis : active site mechanisms and inhibition / / authored by Marco Brito-Arias |
| Autore | Brito Arias Marco Augusto |
| Pubbl/distr/stampa | Singapore : , : Bentham Science Publishers Pte Ltd., , [2020] |
| Descrizione fisica | 1 online resource (334 pages) |
| Disciplina | 572.567 |
| Soggetto topico |
Glycolysis
Amino acids Enzymes - Synthesis |
| ISBN | 981-14-6090-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910807164303321 |
|
Brito Arias Marco Augusto
|
||
| Singapore : , : Bentham Science Publishers Pte Ltd., , [2020] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
GAPDH : biological properties and diversity / / Norbert W. Seidler
| GAPDH : biological properties and diversity / / Norbert W. Seidler |
| Autore | Seidler Norbert W |
| Edizione | [1st ed. 2013.] |
| Pubbl/distr/stampa | Dordrecht ; ; New York, : Springer, 2012, c2013 |
| Descrizione fisica | 1 online resource (303 p.) |
| Disciplina |
616.97
616.97/5 616.975 |
| Collana | Advances in experimental medicine and biology |
| Soggetto topico |
Enzymes - Synthesis
Enzymology Glycolysis |
| ISBN | 94-007-4716-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
GAPDH: Biological Propertiesand Diversity; Contents; Introduction; Chapter 1: Basic Biology of GAPDH; 1.1 The GAPDH Gene; 1.1.1 Coding Region; 1.1.2 Promoter Sequence; 1.1.2.1 Hypoxia-Responsive Elements; 1.1.2.2 Basal Level Expression; 1.1.2.3 Glutamine-Responsive Elements; 1.1.3 Testes-Specific Isoform; 1.1.4 GAPDH Pseudogenes; 1.2 Regulation of GAPDH Expression; 1.2.1 Tissue Specificity; 1.2.2 Tracking GAPDH Information Electronically; 1.2.3 Cancer; 1.3 Cellular Levels of GAPDH; 1.4 Oxidoreductase Activity of GAPDH; 1.4.1 Mechanism of Catalysis; 1.4.2 Kinetic Parameters
1.5 Architecture of the GAPDH Protein1.5.1 Asymmetric Homotetramer; 1.5.2 Dinucleotide Binding Domain; 1.5.3 Catalytic Domain; References; Chapter 2: GAPDH and Intermediary Metabolism; 2.1 GAPDH, the Glycolytic Lynch-Pin; 2.1.1 Metabolic Switch; 2.1.2 Glycolytic Tissues; 2.1.3 Anaerobic Glycolysis; 2.2 Determining GAPDH Activity; 2.2.1 Chemical Inhibitors; 2.2.2 Measurement of Glycolytic Flux; 2.2.3 Oxidoreductase Activity of GAPDH; 2.2.3.1 Conditions of Assay; Buffer Selection; Triethanolamine Buffer; Sodium Phosphate Buffer; GAPDH (rabbit muscle); Reagents; NAD+ stock solution (10mM) d/l-glyceraldehyde stock solution (200mM)2.2.3.2 Assay Protocol; Determine Rate of NAD+ Reduction; Calculate Specific Activity; 2.3 Role of GAPDH Metabolites; 2.3.1 Counter-Catalytic Activity; 2.3.2 Controlling NADH Levels; 2.3.3 Phosphocreatine, as a Competitive Inhibitor; 2.3.4 Metabolic Parameters in the Brain; 2.4 Comparative Analysis; 2.4.1 Structure-Function of NAD+-Binding; 2.4.2 Sequence Homology; References; Chapter 3: Compartmentation of GAPDH; 3.1 Compartmentation of Glycolytic Energy; 3.1.1 Microzones of Cellular ATP; 3.1.2 Focal Regulation of NAD+/NADH Ratios 3.1.3 Channeling of Metabolites3.1.4 Non-glycolytic Compartmentation; 3.2 Binding to the Plasma Membrane; 3.2.1 SLC4 Anion Exchanger; 3.2.1.1 Band 3 in Erythrocytes; 3.2.1.2 Kidney AE1 Isoform; 3.2.2 Na+/K+-ATPase; 3.2.3 ATP-Sensitive K+-Channel; 3.2.4 GLUT Transporters; 3.2.4.1 GLUT1 Transporter in Erythrocytes; 3.2.4.2 GLUT4 Transporter; 3.2.5 GABA (Type A) Receptor; 3.2.6 GAPDH, as a Lactoferrin Receptor; 3.3 Translocation to the Nucleus; 3.4 Other Non-cytosolic Destinations; 3.4.1 Clathrin-Coated Vesicles; 3.4.2 Golgi Apparatus and Endoplasmic Reticulum; 3.4.3 Sarcoplasmic Reticulum 3.4.4 Mitochondria3.5 Dendrites, Axons and Synapses; 3.5.1 Synaptic Vesicles; 3.5.2 Post-synaptic Density; 3.6 Specialized Compartment for Spermatogenic GAPDH; References; Chapter 4: Functional Diversity; 4.1 Classical Example of Protein `Moonlighting ́; 4.1.1 Evolutionary Considerations; 4.1.2 Molecular Mechanisms; 4.2 Structural Organization of the Cell; 4.2.1 Cytoskeletal Components; 4.2.1.1 Actin Filaments; 4.2.1.2 Microtubules; 4.2.2 Organelle Biogenesis; 4.2.2.1 Triadic Junction; 4.2.2.2 Nuclear Envelope; 4.2.2.3 Vesicle Recycling/Membrane Fusion; 4.2.2.4 Cell Polarization 4.2.2.5 Golgi and Endoplasmic Reticulum |
| Record Nr. | UNINA-9910437616403321 |
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Seidler Norbert W
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| Dordrecht ; ; New York, : Springer, 2012, c2013 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Kinetics of Enzymatic Synthesis / / edited by Lakshmanan Rajendran and Carlos Fernandez
| Kinetics of Enzymatic Synthesis / / edited by Lakshmanan Rajendran and Carlos Fernandez |
| Pubbl/distr/stampa | London, United Kingdom : , : IntechOpen, , 2019 |
| Descrizione fisica | 1 online resource (122 pages) : illustrations some color |
| Disciplina | 572.744 |
| Soggetto topico |
Biochemistry
Enzyme kinetics Enzymes - Synthesis |
| ISBN |
1-83881-830-8
1-78985-030-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910317766803321 |
| London, United Kingdom : , : IntechOpen, , 2019 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Organic synthesis with enzymes in non-aqueous media / / edited by Giacomo Carrea and Sergio Riva
| Organic synthesis with enzymes in non-aqueous media / / edited by Giacomo Carrea and Sergio Riva |
| Pubbl/distr/stampa | Weinheim, [Germany] : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2008 |
| Descrizione fisica | 1 online resource (330 p.) |
| Disciplina |
547.02
547.2 |
| Soggetto topico |
Organic compounds - Synthesis
Catalysis Enzymes - Synthesis |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-283-14039-X
9786613140395 3-527-62172-5 3-527-62173-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Organic Synthesis with Enzymes in Non-Aqueous Media; Contents; Preface; List of Contributors; Part One Biocatalysis in Neat Organic Solvents - Fundamentals; 1 Fundamentals of Biocatalysis in Neat Organic Solvents; 1.1 Introduction; 1.2 Effects of Water on Biocatalytic Reactions; 1.2.1 Quantification of Water in Low-Water Systems; 1.2.2 Water Activity Control; 1.2.2.1 Water Activity Control Using Saturated Salt Solutions; 1.2.2.2 Water Activity Control Using Sensors; 1.2.2.3 Water Activity Control Using Pairs of Salt Hydrates; 1.2.3 Distribution of Water; 1.2.3.1 Hysteresis Effects
1.2.4 Water Effects on Activity1.2.5 Water Effects on Selectivity; 1.2.6 Water Effects on Stability; 1.3 Solvent Effects; 1.3.1 Solvent Effects on Enzyme Activity; 1.3.2 Solvent Effects on Stability; 1.4 Effects on Equilibria; 1.4.1 Water Effects on Equilibria; 1.4.2 Solvent Effects on Equilibria; 1.5 Effects of pH in Organic Solvents; 1.6 Concluding Remarks; 2 Effects of Organic Solvents on Enzyme Selectivity; 2.1 Introduction; 2.2 Enzyme Enantioselectivity; 2.3 Effects of Organic Solvents on the E-value; 2.4 Possible Causes of the Complexity of Solvent Effects on E 2.5 The Accuracy of E-value Determinations2.6 Kinetic and Thermodynamic Analysis of the Specificity Constants; 2.7 Solvents Effects on Non-Hydrolytic Enzymes; 2.8 Major Achievements; 2.9 Concluding Remarks; 3 Activating Enzymes for Use in Organic Solvents; 3.1 Introduction; 3.2 Water - A Unique and Necessary Solvent for Enzymatic Catalysis; 3.2.1 Challenges for Enzymatic Catalysis in Water; 3.2.2 Enzymes do Function Without Water as a Bulk Solvent - Lessons from Extreme Halophiles; 3.2.3 Behavior of Enzymes in the Absence of Bulk Water 3.2.4 Removing Water from Enzymes - the Effect of Lyophilization on Enzyme Structure and Function3.3 Enzyme Activation in Nonaqueous Media; 3.3.1 Addition of Water and Water Mimics; 3.3.2 Immobilization; 3.3.3 Solid-State Buffers; 3.3.4 Lyophilization in the Presence of Excipients; 3.3.4.1 Polymers; 3.3.4.2 Crown Ethers and Cyclodextrins; 3.4 Salt-Activated Enzymes; 3.4.1 Salt Activation is not due to a Relaxation of Diffusional Limitations; 3.4.2 Mechanism of Salt Activation; 3.4.3 The Structural and Molecular Dynamics of Salt Activation; 3.5 Conclusions Part Two Biocatalysis in Neat Organic Solvents - Synthetic Applications4 Exploiting Enantioselectivity of Hydrolases in Organic Solvents; 4.1 Introduction; 4.1.1 Enantioselectivity; 4.1.2 Desymmetrization; 4.1.3 The Reversibility Problem; 4.1.4 Determining and Optimizing Enantioselectivity; 4.2 Enantioselective Reactions in Organic Solvents; 4.2.1 Reactions of Alcohols; 4.2.1.1 Primary Alcohols; 4.2.1.2 Secondary Alcohols; 4.2.1.3 Tertiary Alcohols; 4.2.1.4 Resolution of Dihydroxybiaryls; 4.2.2 Reaction of Amines; 4.2.2.1 Alkylamines; 4.2.2.2 Arylalkylamines; 4.2.2.3 Amino Acid Derivatives 4.2.3 Reaction of Acid Derivatives |
| Record Nr. | UNINA-9910144376003321 |
| Weinheim, [Germany] : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2008 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Organic synthesis with enzymes in non-aqueous media / / edited by Giacomo Carrea and Sergio Riva
| Organic synthesis with enzymes in non-aqueous media / / edited by Giacomo Carrea and Sergio Riva |
| Pubbl/distr/stampa | Weinheim, [Germany] : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2008 |
| Descrizione fisica | 1 online resource (330 p.) |
| Disciplina |
547.02
547.2 |
| Soggetto topico |
Organic compounds - Synthesis
Catalysis Enzymes - Synthesis |
| ISBN |
1-283-14039-X
9786613140395 3-527-62172-5 3-527-62173-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Organic Synthesis with Enzymes in Non-Aqueous Media; Contents; Preface; List of Contributors; Part One Biocatalysis in Neat Organic Solvents - Fundamentals; 1 Fundamentals of Biocatalysis in Neat Organic Solvents; 1.1 Introduction; 1.2 Effects of Water on Biocatalytic Reactions; 1.2.1 Quantification of Water in Low-Water Systems; 1.2.2 Water Activity Control; 1.2.2.1 Water Activity Control Using Saturated Salt Solutions; 1.2.2.2 Water Activity Control Using Sensors; 1.2.2.3 Water Activity Control Using Pairs of Salt Hydrates; 1.2.3 Distribution of Water; 1.2.3.1 Hysteresis Effects
1.2.4 Water Effects on Activity1.2.5 Water Effects on Selectivity; 1.2.6 Water Effects on Stability; 1.3 Solvent Effects; 1.3.1 Solvent Effects on Enzyme Activity; 1.3.2 Solvent Effects on Stability; 1.4 Effects on Equilibria; 1.4.1 Water Effects on Equilibria; 1.4.2 Solvent Effects on Equilibria; 1.5 Effects of pH in Organic Solvents; 1.6 Concluding Remarks; 2 Effects of Organic Solvents on Enzyme Selectivity; 2.1 Introduction; 2.2 Enzyme Enantioselectivity; 2.3 Effects of Organic Solvents on the E-value; 2.4 Possible Causes of the Complexity of Solvent Effects on E 2.5 The Accuracy of E-value Determinations2.6 Kinetic and Thermodynamic Analysis of the Specificity Constants; 2.7 Solvents Effects on Non-Hydrolytic Enzymes; 2.8 Major Achievements; 2.9 Concluding Remarks; 3 Activating Enzymes for Use in Organic Solvents; 3.1 Introduction; 3.2 Water - A Unique and Necessary Solvent for Enzymatic Catalysis; 3.2.1 Challenges for Enzymatic Catalysis in Water; 3.2.2 Enzymes do Function Without Water as a Bulk Solvent - Lessons from Extreme Halophiles; 3.2.3 Behavior of Enzymes in the Absence of Bulk Water 3.2.4 Removing Water from Enzymes - the Effect of Lyophilization on Enzyme Structure and Function3.3 Enzyme Activation in Nonaqueous Media; 3.3.1 Addition of Water and Water Mimics; 3.3.2 Immobilization; 3.3.3 Solid-State Buffers; 3.3.4 Lyophilization in the Presence of Excipients; 3.3.4.1 Polymers; 3.3.4.2 Crown Ethers and Cyclodextrins; 3.4 Salt-Activated Enzymes; 3.4.1 Salt Activation is not due to a Relaxation of Diffusional Limitations; 3.4.2 Mechanism of Salt Activation; 3.4.3 The Structural and Molecular Dynamics of Salt Activation; 3.5 Conclusions Part Two Biocatalysis in Neat Organic Solvents - Synthetic Applications4 Exploiting Enantioselectivity of Hydrolases in Organic Solvents; 4.1 Introduction; 4.1.1 Enantioselectivity; 4.1.2 Desymmetrization; 4.1.3 The Reversibility Problem; 4.1.4 Determining and Optimizing Enantioselectivity; 4.2 Enantioselective Reactions in Organic Solvents; 4.2.1 Reactions of Alcohols; 4.2.1.1 Primary Alcohols; 4.2.1.2 Secondary Alcohols; 4.2.1.3 Tertiary Alcohols; 4.2.1.4 Resolution of Dihydroxybiaryls; 4.2.2 Reaction of Amines; 4.2.2.1 Alkylamines; 4.2.2.2 Arylalkylamines; 4.2.2.3 Amino Acid Derivatives 4.2.3 Reaction of Acid Derivatives |
| Record Nr. | UNINA-9910829909603321 |
| Weinheim, [Germany] : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2008 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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