Pubbl/distr/stampa	London, England : , : Future Science Ltd, , 2013
Descrizione fisica	1 online resource (145 pages) : color illustrations, photographs, tables
Disciplina	615.1901
Collana	Future Science
Soggetto topico	Drugs - Analysis Biomolecules - Analysis Microchemistry
ISBN	1-909453-57-9 1-909453-58-7
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Record Nr.	UNINA-9910789326803321

Autore	Dong M. W
Pubbl/distr/stampa	Hoboken, N.J., : Wiley-Interscience, 2006
Descrizione fisica	1 online resource (306 p.)
Disciplina	543.0894 615.19 615/.19
Soggetto topico	High performance liquid chromatography Drugs - Analysis
ISBN	1-119-29360-X 1-280-45047-9 9786610450473 0-470-24575-1 0-471-97310-6 0-471-97309-2
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Nota di contenuto	MODERN HPLC FOR PRACTICING SCIENTISTS; CONTENTS; Preface; 1 Introduction; 1.1 Introduction; 1.1.1 Scope; 1.1.2 What Is HPLC?; 1.1.3 A Brief History; 1.1.4 Advantages and Limitations; 1.2 Modes of HPLC; 1.2.1 Normal-Phase Chromatography (NPC); 1.2.2 Reversed-Phase Chromatography (RPC); 1.2.3 Ion-Exchange Chromatography (IEC); 1.2.4 Size-Exclusion Chromatography (SEC); 1.2.5 Other Separation Modes; 1.3 Some Common-Sense Corollaries; 1.4 How to Get More Information; 1.5 Summary; 1.6 References; 2 Basic Terms and Concepts; 2.1 Scope; 2.2 Basic Terms and Concepts 2.2.1 Retention Time (t(R)), Void Time (t(M)), Peak Height (h), and Peak Width (w(b))2.2.2 Retention Volume (V(R)), Void Volume (V(M)), and Peak Volume; 2.2.3 Retention Factor (k); 2.2.4 Separation Factor (a); 2.2.5 Column Efficiency and Plate Number (N); 2.2.6 Peak Volume; 2.2.7 Height Equivalent to a Theoretical Plate or Plate Height (HETP or H); 2.2.8 Resolution (R(s)); 2.2.9 Peak Symmetry: Asymmetry Factor (A(s)) and Tailing Factor (T(f)); 2.3 Mobile Phase; 2.3.1 General Requirements; 2.3.2 Solvent Strength and Selectivity; 2.3.3 Buffers; 2.3.4 Acidic Mobile Phases 2.3.5 Ion-Pairing Additives2.3.6 High pH Mobile Phase; 2.3.7 Other Operating Parameters: Flow Rate (F) and Column Temperature (T); 2.4 The Resolution Equation; 2.5 The Van Deemter Equation; 2.6 Isocratic vs. Gradient Analysis; 2.6.1 Peak Capacity (n); 2.6.2 Key Gradient Parameters (Initial and Final Solvent Strength, Gradient Time [t(G)], and Flow Rate); 2.6.3 The 0.25Dt(G) Rule:When Is Isocratic Analysis More Appropriate?; 2.7 Concept of Orthogonality; 2.8 Sample Capacity; 2.9 Glossary of HPLC Terms; 2.10 Summary and Conclusion; 2.11 References; 3 HPLC Columns and Trends; 3.1 Scope 3.2 General Column Description and Characteristics 3.2.1 Column Hardware-Standard vs. Cartridge Format; 3.3 Column Types; 3.3.1 Types Based on Chromatographic Modes; 3.3.2 Types Based on Dimensions; 3.3.3 Column Length (L); 3.4 Column Packing Characteristics; 3.4.1 Support Type; 3.4.2 Particle Size (d(p)); 3.4.3 Surface Area and Pore Size (d(pore)); 3.4.4 Bonding Chemistries; 3.4.5 Some General Guidelines for Bonded Phase Selection; 3.5 Modern HPLC Column Trends; 3.5.1 High-Purity Silica; 3.5.2 Hybrid Particles; 3.5.3 Novel Bonding Chemistries; 3.5.4 Fast LC; 3.5.5 Micro LC; 3.5.6 Monoliths 3.6 Guard Columns3.7 Specialty Columns; 3.7.1 Bioseparation Columns; 3.7.2 Chiral Columns; 3.7.3 Application-Specific Columns; 3.8 Column Selection Guides; 3.9 Summary; 3.10 References; 3.11 Internet Resources; 4 HPLC Instrumentation and Trends; 4.1 Introduction; 4.1.1 Scope; 4.1.2 HPLC Systems and Modules; 4.2 HPLC Solvent Delivery Systems; 4.2.1 High-Pressure and Low-Pressure Mixing Designs in Multisolvent Pumps; 4.2.2 System Dwell Volume; 4.2.3 Trends; 4.3 Injectors and Autosamplers; 4.3.1 Operating Principles of Autosamplers; 4.3.2 Performance Characteristics and Trends; 4.4 Detectors 4.5 UV/VIS Absorbance Detectors
Record Nr.	UNINA-9910143397603321

Autore	Ali Imran
Pubbl/distr/stampa	Hoboken, N.J., : John Wiley & Sons, c2009
Descrizione fisica	1 online resource (288 p.)
Disciplina	543.8 543/.8
Altri autori (Persone)	Aboul-EneinHassan Y GuptaVinod K. <1953->
Soggetto topico	Chromatographic analysis Capillary electrophoresis Drugs - Analysis Pollutants - Analysis Nanoparticles - Analysis
Soggetto genere / forma	Electronic books.
ISBN	1-282-11320-8 9786612113208 0-470-43492-9 1-61583-174-6 0-470-43491-0
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Nota di contenuto	NANOCHROMATOGRAPHY AND NANOCAPILLARY ELECTROPHORESIS; CONTENTS; PREFACE; ACKNOWLEDGMENTS; 1 Introduction; 1.1. Nanoanalyses; 1.2. Definition of Nanochromatography and Nanocapillary Electrophoresis; 1.3. Nanochromatography and Nanocapillary Electrophoresis; 1.4. Fabrication of Microdevices; 1.5. Developments in Nanoanalyses; 1.6. Data Integration; 1.7. Protocol of Nanoanalyses; 1.8. Scope of the Book; 1.9. Conclusion; References; 2 Fabrication of Microchips; 2.1. Introduction; 2.2. Substrates; 2.3. Techniques of Fabrication; 2.3.1. Glass Chips; 2.3.2. Quartz Chips; 2.3.3. Silica Chips 2.3.4. Polymer Chips2.3.5. Plastic Chips; 2.3.6. Chips and the Polymerase Chain Reaction; 2.4. Surface Modification; 2.4.1. Modification by Polymers; 2.4.2. Modification by Silica Gel; 2.4.3. Modification by Monolithics; 2.4.4. Modification by Sulfonation; 2.5. Designs of Chips; 2.6. Bindings in Chips; 2.7. Conclusion; References; 3 Instrumentation of Nanochromatography and Nanocapillary Electrophoresis; 3.1. Introduction; 3.2. Nanoliquid Chromatography (NLC); 3.2.1. Mobile Phase Reservoirs; 3.2.2. Mobile Phases and Flow Calibration; 3.2.3. Mobile Phase Tubings; 3.2.4. Solvent Delivery Pump 3.2.5. Sample Injector3.2.6. Separation Chips; 3.2.7. Detectors; 3.2.8. Recorder; 3.2.9. Sample Preparation Units; 3.3. Nanocapillary Electrophoresis; 3.3.1. Separation Chip; 3.3.2. Background Electrolyte and Its Reservoirs; 3.3.3. Sample Injection Port; 3.3.4. Detectors; 3.3.5. Recorder; 3.3.6. Sample Preparation Units; 3.4. Conclusion; References; 4 Detection in Nanochromatography and Nanocapillary Electrophoresis; 4.1. Introduction; 4.2. Mass Spectrometer Detectors; 4.3. Fluorescence Detectors; 4.4. Electrochemical Detectors; 4.5. Element Specific Detectors; 4.6. Miscellaneous Detectors 4.7. ConclusionReferences; 5 Sample Preparation in Nanochromatography and Nanocapillary Electrophoresis; 5.1. Introduction; 5.2. Sample Preparation; 5.3. Sampling; 5.3.1. Biological Samples; 5.3.1.1. Blood; 5.3.1.2. Urine; 5.3.1.3. Tissues; 5.3.1.4. Plants; 5.3.1.5. Food Stuffs; 5.3.2. Environmental Samples; 5.3.2.1. Air; 5.3.2.2. Water; 5.3.2.3. Sediment and Soils; 5.4. Preservation; 5.5. Filtration; 5.6. Digestion/Homogenization; 5.7. Extractions; 5.8. Clean Up; 5.9. Preconcentration; 5.10. Off-Line Nanosample Preparation Methods; 5.10.1. Nano Solid Phase Extractions 5.10.2. Nano Membrane Extractions5.10.3. Nano Miscellaneous Extractions; 5.11. Online Nanosample Preparation Methods; 5.12. Conclusion; References; 6 Nano-High Performance Liquid Chromatography; 6.1. Introduction; 6.2. Nano-HPLC; 6.3. Applications; 6.3.1. Nano-HPLC of Biological Matrices; 6.3.1.1. Proteomics; 6.3.1.2. Drugs Development and Design; 6.3.1.3. Environmental Analysis; 6.4. Optimization of Separations in Nano-HPLC; 6.5. Troubleshooting in Nano-HPLC; 6.6. Conclusion; References; 7 Nanocapillary Electrochromatography and Nanomicellar Electrokinetic Chromatography; 7.1. Introduction 7.2. Nanocapillary Electrochromatography
Record Nr.	UNINA-9910145961203321