Methods in bioengineering : systems analysis of biological networks / / Arul Jayaraman, Juergen Hahn, editors |
Pubbl/distr/stampa | Boston [Mass.] : , : Artech House, , ©2009 |
Descrizione fisica | 1 online resource (328 p.) |
Disciplina |
610.28
660.6 |
Altri autori (Persone) |
JayaramanArul
HahnJuergen |
Collana | Artech House methods in bioengineering series |
Soggetto topico |
Bacterial genetics
Cytology - Technique Viral genetics |
ISBN |
1-5231-4635-4
1-59693-407-7 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Methods in Bioengineering: Systems Analysis of Biological Networks; Contents; Chapter 1 Quantitative Immunofluorescence for Measuring Spatial Compartmentation of Covalently Modified Signaling Proteins; 1.1 Introduction; 1.2 Experimental Design; 1.3 Materials; 1.3.1 Cell culture; 1.3.2 Buffers/reagents; 1.3.3 Immunofluorescence reagents; 1.4 Methods; 1.4.1 Cell culture and stimulation for phospho-ERK measurements; 1.4.2 Antibody labeling of phosphorylated ERK (ppERK); 1.4.3 Fluorescence microscopy imaging of ppERK and automated imageanalysis
1.5 Data Acquisition, Anticipated Results, and Interpretation1.6 Statistical Guidelines; 1.7 Discussion and Commentary; 1.8 Application Notes; 1.9 Summary Points; Acknowledgments; References; Chapter 2 Development of Green Fluorescent Protein-Based Reporter Cell Lines for Dynamic Profiling of Transcription Factor and Kinase Activation; 2.1 Introduction; 2.2 Materials; 2.2.1 Cell and bacterial culture; 2.2.2 Buffers and reagents; 2.2.3 Cloning; 2.2.4 Microscopy; 2.3 Methods; 2.3.1 3T3-L1 cell culture; 2.3.2 Transcription factor reporter development; 2.3.3 Kinase reporter development 2.4 Application Notes2.4.1 Electroporation of TF reporter plasmids into 3T3-L1 preadipocytes; 2.4.2 Monitoring activation of ERK in HepG2 cells; 2.5 Data Acquisition, Anticipated Results, and Interpretation; 2.6 Discussion and Commentary; 2.7 Summary Points; Acknowledgments; References; Chapter 3 Comparison of Algorithms for Analyzing Fluorescent Microscopy Images and Computation of Transcription Factor Profiles; 3.1 Introduction; 3.2 Preliminaries; 3.2.1 Principles of GFP reporter systems; 3.2.2 Wavelets; 3.2.3 K-means clustering; 3.2.4 Principal component analysis 3.2.5 Mathematical description of digital images and image analysis3.3 Methods; 3.3.1 Image analysis based on wavelets and a bidirectional search; 3.3.2 Image analysis based on K-means clustering and PCA; 3.3.3 Determining fluorescence intensity of an image; 3.3.4 Comparison of the two image analysis procedures; 3.4 Data Acquisition, Anticipated Results, and Interpretation; 3.4.1 Developing a model describing the relationship between the transcription factor concentration and the observed fluorescence intensity 3.4.2 Solution of an inverse problem for determining transcription factor concentrations3.5 Application Notes; 3.6 Summary and Conclusions; Acknowledgments; References; Chapter 4 Data-Driven, Mechanistic Modeling of Biochemical Reaction Networks; 4.1 Introduction; 4.2 Principles of Data-Driven Modeling; 4.2.1 Types of experimental data; 4.2.2 Data processing and normalization; 4.2.3 Suitability of models used in conjunction with quantitative data; 4.2.4 Issues related to parameter specification and estimation; 4.3 Examples of Data-Driven Modeling 4.3.1 Example 1: Systematic analysis of crosstalk in the PDGF receptor signaling network |
Altri titoli varianti | Systems analysis of biological networks |
Record Nr. | UNINA-9910780809003321 |
Boston [Mass.] : , : Artech House, , ©2009 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Methods in bioengineering : systems analysis of biological networks / / Arul Jayaraman, Juergen Hahn, editors |
Pubbl/distr/stampa | Boston [Mass.] : , : Artech House, , ©2009 |
Descrizione fisica | 1 online resource (328 p.) |
Disciplina |
610.28
660.6 |
Altri autori (Persone) |
JayaramanArul
HahnJuergen |
Collana | Artech House methods in bioengineering series |
Soggetto topico |
Bacterial genetics
Cytology - Technique Viral genetics |
ISBN |
1-5231-4635-4
1-59693-407-7 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Methods in Bioengineering: Systems Analysis of Biological Networks; Contents; Chapter 1 Quantitative Immunofluorescence for Measuring Spatial Compartmentation of Covalently Modified Signaling Proteins; 1.1 Introduction; 1.2 Experimental Design; 1.3 Materials; 1.3.1 Cell culture; 1.3.2 Buffers/reagents; 1.3.3 Immunofluorescence reagents; 1.4 Methods; 1.4.1 Cell culture and stimulation for phospho-ERK measurements; 1.4.2 Antibody labeling of phosphorylated ERK (ppERK); 1.4.3 Fluorescence microscopy imaging of ppERK and automated imageanalysis
1.5 Data Acquisition, Anticipated Results, and Interpretation1.6 Statistical Guidelines; 1.7 Discussion and Commentary; 1.8 Application Notes; 1.9 Summary Points; Acknowledgments; References; Chapter 2 Development of Green Fluorescent Protein-Based Reporter Cell Lines for Dynamic Profiling of Transcription Factor and Kinase Activation; 2.1 Introduction; 2.2 Materials; 2.2.1 Cell and bacterial culture; 2.2.2 Buffers and reagents; 2.2.3 Cloning; 2.2.4 Microscopy; 2.3 Methods; 2.3.1 3T3-L1 cell culture; 2.3.2 Transcription factor reporter development; 2.3.3 Kinase reporter development 2.4 Application Notes2.4.1 Electroporation of TF reporter plasmids into 3T3-L1 preadipocytes; 2.4.2 Monitoring activation of ERK in HepG2 cells; 2.5 Data Acquisition, Anticipated Results, and Interpretation; 2.6 Discussion and Commentary; 2.7 Summary Points; Acknowledgments; References; Chapter 3 Comparison of Algorithms for Analyzing Fluorescent Microscopy Images and Computation of Transcription Factor Profiles; 3.1 Introduction; 3.2 Preliminaries; 3.2.1 Principles of GFP reporter systems; 3.2.2 Wavelets; 3.2.3 K-means clustering; 3.2.4 Principal component analysis 3.2.5 Mathematical description of digital images and image analysis3.3 Methods; 3.3.1 Image analysis based on wavelets and a bidirectional search; 3.3.2 Image analysis based on K-means clustering and PCA; 3.3.3 Determining fluorescence intensity of an image; 3.3.4 Comparison of the two image analysis procedures; 3.4 Data Acquisition, Anticipated Results, and Interpretation; 3.4.1 Developing a model describing the relationship between the transcription factor concentration and the observed fluorescence intensity 3.4.2 Solution of an inverse problem for determining transcription factor concentrations3.5 Application Notes; 3.6 Summary and Conclusions; Acknowledgments; References; Chapter 4 Data-Driven, Mechanistic Modeling of Biochemical Reaction Networks; 4.1 Introduction; 4.2 Principles of Data-Driven Modeling; 4.2.1 Types of experimental data; 4.2.2 Data processing and normalization; 4.2.3 Suitability of models used in conjunction with quantitative data; 4.2.4 Issues related to parameter specification and estimation; 4.3 Examples of Data-Driven Modeling 4.3.1 Example 1: Systematic analysis of crosstalk in the PDGF receptor signaling network |
Altri titoli varianti | Systems analysis of biological networks |
Record Nr. | UNINA-9910811080903321 |
Boston [Mass.] : , : Artech House, , ©2009 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Metodi nella citogenetica umana / Hans G. Schwarzacher, Ulrich Wolf ; tr. di Aureliano Rovescalli |
Autore | Schwarzacher, Hans G. |
Pubbl/distr/stampa | Padova : Piccin, c1978 |
Descrizione fisica | xv, 290 p. : ill. ; 22 cm |
Disciplina | 572.8 |
Altri autori (Persone) | Wolf, Ulrichauthor |
Soggetto topico |
Cytodiagnostics
Cytogenetics Cytology - Technique Human cell culture |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | ita |
Record Nr. | UNISALENTO-991003516719707536 |
Schwarzacher, Hans G. | ||
Padova : Piccin, c1978 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. del Salento | ||
|
Practical cell analysis [[electronic resource] /] / Dimitri Pappas |
Autore | Pappas Dimitri |
Pubbl/distr/stampa | Hoboken, N.J., : Wiley, 2010 |
Descrizione fisica | 1 online resource (316 p.) |
Disciplina | 571.6028 |
Soggetto topico |
Cytology - Technique
Biology |
ISBN |
1-282-48223-8
9786612482236 0-470-68844-0 0-470-68845-9 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Practical Cell Analysis; Contents; Preface; Acknowledgments; 1 Getting Started (and Getting the Cells); 1.1 INTRODUCTION; 1.2 THE DRIVING NEED; 1.3 PRIMARY AND CULTURED CELLS; 1.4 CHOOSING A CULTURED CELL; 1.5 CHOOSING PRIMARY CELLS; 1.6 EASILY OBTAINABLE PRIMARY CELLS; 1.7 PRIMARY CELLS FROM TISSUES; 1.8 PURIFYING PRIMARY CELLS; 1.9 HOW LONG DO PRIMARY CELLS REMAIN PRIMARY?; 1.10 OBTAINING PRIMARY CELLS FROM A COMMERCIAL SOURCE; 1.11 BACTERIA AND YEAST; 1.12 PRACTICAL ASPECTS OF CELL CULTURE; 1.13 SAFETY ASPECTS OF PRIMARY AND TRANSFORMED CELL LINES
1.14 TRANSFECTION OF PRIMARY AND TRANSFORMED CELL LINES1.15 CONCLUSION; REFERENCES; 2 The Cell-Culture Laboratory (Tools of the Trade); 2.1 INTRODUCTION; 2.2 ISSUES CONCERNING A CELL LABORATORY; 2.3 SETTING UP A CELL CULTURE LABORATORY; 2.4 CELL LINE STORAGE; 2.5 PERSONAL PROTECTIVE EQUIPMENT; 2.6 CELL AND SAMPLE HANDLING; 2.7 COMMON ANALYTICAL INSTRUMENTATION FOR CELL CULTURE; 2.8 CONSIDERATIONS WHEN SETTING UP A CELL-CULTURE LABORATORY; 2.9 ESTABLISHING AND REGULATING A CULTURE FACILITY; 2.10 CONCLUSION; REFERENCES; 3 Maintaining Cultures; 3.1 INTRODUCTION; 3.2 MEDIUM 3.3 THE USE OF MEDIUM IN ANALYSIS, AND ALTERNATIVES3.4 CULTURING CELLS; 3.5 GROWING CELLS IN THREE DIMENSIONS; 3.6 STERILITY AND CONTAMINATION OF CULTURE; 3.7 STORAGE OF CELL SAMPLES AND CELL LINES; PROTOCOL 3.2: CRYOPRESERVATION OF MAMMALIAN CELLS; PROTOCOL 3.3: RETRIEVAL OF CELLS FROM LIQUID-NITROGEN STORAGE; 3.8 CONCLUSION; REFERENCES; 4 Microscopy of Cells; 4.1 INTRODUCTION; 4.2 MICROSCOPE TYPES; 4.3 CULTURING CELLS FOR MICROSCOPY; 4.4 SIGNALS, BACKGROUND, AND ARTIFACTS IN OPTICAL MICROSCOPY; 4.5 STAINING CELLS FOR FLUORESCENCE MICROSCOPY PROTOCOL 4.1 FIXATION OF CELLS FOR IMMUNOCHEMICAL STAINING4.6 MULTIPLE LABELS; 4.7 VIABILITY AND TWO-PHOTON MICROSCOPY; 4.8 SPATIAL RESOLUTION IN OPTICAL MICROSCOPY; 4.9 IMAGE SATURATION AND INTENSITY; 4.10 ATOMIC FORCE AND ENVIRONMENTAL SCANNING ELECTRON MICROSCOPY; 4.11 CONCLUSION; REFERENCES; 5 Separating Cells; 5.1 INTRODUCTION; 5.2 THE CELL SAMPLE; 5.3 LABEL-FREE (INTRINSIC) SEPARATIONS; 5.4 IMMUNOMAGNETIC SORTING; 5.5 CELL-AFFINITY CHROMATOGRAPHY; 5.6 AFFINITY CHEMISTRY CONSIDERATIONS IN CAC AND MACS SEPARATIONS; PROTOCOL 5.1: SCREENING OF ANTIBODY CLONES 5.7 ELUTION IN CELL-AFFINITY CHROMATOGRAPHY5.8 NONSPECIFIC BINDING IN CELL SEPARATIONS; 5.9 SEPARATION OF RARE CELLS; 5.10 FLUORESCENCE-ACTIVATED CELL SORTING; 5.11 SORTING PARAMETERS; 5.12 OTHER SEPARATION TECHNIQUES AND CONSIDERATIONS; 5.13 CONCLUSION; REFERENCES; 6 Flow Cytometry: Cell Analysis in the Fast Lane; 6.1 INTRODUCTION; 6.2 THE CELL SAMPLE; 6.3 FLOW CYTOMETER FUNCTION; 6.4 OBTAINING OR FINDING A FLOW CYTOMETER; 6.5 USING FLOW CYTOMETERS; 6.6 SETTING UP A FLOW CYTOMETER FOR MULTI-COLOR STAINING; 6.7 ANALYZING FLOW CYTOMETRY DATA; 6.8 EXAMPLE FLOW-CYTOMETRY ASSAYS 6.9 NO-FLOW CYTOMETRY |
Record Nr. | UNINA-9910139503403321 |
Pappas Dimitri | ||
Hoboken, N.J., : Wiley, 2010 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Practical cell analysis [[electronic resource] /] / Dimitri Pappas |
Autore | Pappas Dimitri |
Pubbl/distr/stampa | Hoboken, N.J., : Wiley, 2010 |
Descrizione fisica | 1 online resource (316 p.) |
Disciplina | 571.6028 |
Soggetto topico |
Cytology - Technique
Biology |
ISBN |
1-282-48223-8
9786612482236 0-470-68844-0 0-470-68845-9 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Practical Cell Analysis; Contents; Preface; Acknowledgments; 1 Getting Started (and Getting the Cells); 1.1 INTRODUCTION; 1.2 THE DRIVING NEED; 1.3 PRIMARY AND CULTURED CELLS; 1.4 CHOOSING A CULTURED CELL; 1.5 CHOOSING PRIMARY CELLS; 1.6 EASILY OBTAINABLE PRIMARY CELLS; 1.7 PRIMARY CELLS FROM TISSUES; 1.8 PURIFYING PRIMARY CELLS; 1.9 HOW LONG DO PRIMARY CELLS REMAIN PRIMARY?; 1.10 OBTAINING PRIMARY CELLS FROM A COMMERCIAL SOURCE; 1.11 BACTERIA AND YEAST; 1.12 PRACTICAL ASPECTS OF CELL CULTURE; 1.13 SAFETY ASPECTS OF PRIMARY AND TRANSFORMED CELL LINES
1.14 TRANSFECTION OF PRIMARY AND TRANSFORMED CELL LINES1.15 CONCLUSION; REFERENCES; 2 The Cell-Culture Laboratory (Tools of the Trade); 2.1 INTRODUCTION; 2.2 ISSUES CONCERNING A CELL LABORATORY; 2.3 SETTING UP A CELL CULTURE LABORATORY; 2.4 CELL LINE STORAGE; 2.5 PERSONAL PROTECTIVE EQUIPMENT; 2.6 CELL AND SAMPLE HANDLING; 2.7 COMMON ANALYTICAL INSTRUMENTATION FOR CELL CULTURE; 2.8 CONSIDERATIONS WHEN SETTING UP A CELL-CULTURE LABORATORY; 2.9 ESTABLISHING AND REGULATING A CULTURE FACILITY; 2.10 CONCLUSION; REFERENCES; 3 Maintaining Cultures; 3.1 INTRODUCTION; 3.2 MEDIUM 3.3 THE USE OF MEDIUM IN ANALYSIS, AND ALTERNATIVES3.4 CULTURING CELLS; 3.5 GROWING CELLS IN THREE DIMENSIONS; 3.6 STERILITY AND CONTAMINATION OF CULTURE; 3.7 STORAGE OF CELL SAMPLES AND CELL LINES; PROTOCOL 3.2: CRYOPRESERVATION OF MAMMALIAN CELLS; PROTOCOL 3.3: RETRIEVAL OF CELLS FROM LIQUID-NITROGEN STORAGE; 3.8 CONCLUSION; REFERENCES; 4 Microscopy of Cells; 4.1 INTRODUCTION; 4.2 MICROSCOPE TYPES; 4.3 CULTURING CELLS FOR MICROSCOPY; 4.4 SIGNALS, BACKGROUND, AND ARTIFACTS IN OPTICAL MICROSCOPY; 4.5 STAINING CELLS FOR FLUORESCENCE MICROSCOPY PROTOCOL 4.1 FIXATION OF CELLS FOR IMMUNOCHEMICAL STAINING4.6 MULTIPLE LABELS; 4.7 VIABILITY AND TWO-PHOTON MICROSCOPY; 4.8 SPATIAL RESOLUTION IN OPTICAL MICROSCOPY; 4.9 IMAGE SATURATION AND INTENSITY; 4.10 ATOMIC FORCE AND ENVIRONMENTAL SCANNING ELECTRON MICROSCOPY; 4.11 CONCLUSION; REFERENCES; 5 Separating Cells; 5.1 INTRODUCTION; 5.2 THE CELL SAMPLE; 5.3 LABEL-FREE (INTRINSIC) SEPARATIONS; 5.4 IMMUNOMAGNETIC SORTING; 5.5 CELL-AFFINITY CHROMATOGRAPHY; 5.6 AFFINITY CHEMISTRY CONSIDERATIONS IN CAC AND MACS SEPARATIONS; PROTOCOL 5.1: SCREENING OF ANTIBODY CLONES 5.7 ELUTION IN CELL-AFFINITY CHROMATOGRAPHY5.8 NONSPECIFIC BINDING IN CELL SEPARATIONS; 5.9 SEPARATION OF RARE CELLS; 5.10 FLUORESCENCE-ACTIVATED CELL SORTING; 5.11 SORTING PARAMETERS; 5.12 OTHER SEPARATION TECHNIQUES AND CONSIDERATIONS; 5.13 CONCLUSION; REFERENCES; 6 Flow Cytometry: Cell Analysis in the Fast Lane; 6.1 INTRODUCTION; 6.2 THE CELL SAMPLE; 6.3 FLOW CYTOMETER FUNCTION; 6.4 OBTAINING OR FINDING A FLOW CYTOMETER; 6.5 USING FLOW CYTOMETERS; 6.6 SETTING UP A FLOW CYTOMETER FOR MULTI-COLOR STAINING; 6.7 ANALYZING FLOW CYTOMETRY DATA; 6.8 EXAMPLE FLOW-CYTOMETRY ASSAYS 6.9 NO-FLOW CYTOMETRY |
Record Nr. | UNINA-9910811952703321 |
Pappas Dimitri | ||
Hoboken, N.J., : Wiley, 2010 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Preparation and characterisation of mammalian plasma membranes / W. R. Howard Evans |
Autore | Evans, W. Howard |
Pubbl/distr/stampa | Amsterdam : Elsevier, c1978 |
Descrizione fisica | 266 p. : ill. ; 20 cm |
Disciplina | 572 |
Collana | Laboratory techniques in biochemistry and molecular biology ; V.7 Pt. 1 |
Soggetto topico |
Cell membranes
Cytology - Technique Cytology (Cell biology) Mammals - Cytology |
ISBN | 0720442222 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNISALENTO-991003546619707536 |
Evans, W. Howard | ||
Amsterdam : Elsevier, c1978 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. del Salento | ||
|
Rapid peptide reagent isolation in a disposable microfluidic cartridge [[electronic resource] /] / Dimitra N. Stratis-Cullum, Joshua M. Kogot, and Paul M. Pellegrino |
Autore | Stratis-Cullum Dimitra N |
Pubbl/distr/stampa | Adelphi, MD : , : Army Research Laboratory, , [2010] |
Descrizione fisica | 1 online resource (viii, 22 pages) : color illustrations |
Altri autori (Persone) |
KogotJoshua M
PellegrinoPaul M |
Collana | ARL-TR |
Soggetto topico |
Cytology - Technique
Peptides Biological reagents Biological weapons |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNINA-9910701150303321 |
Stratis-Cullum Dimitra N | ||
Adelphi, MD : , : Army Research Laboratory, , [2010] | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Technology platforms for 3D cell culture : a user's guide / / edited by Stefan Przyborski |
Pubbl/distr/stampa | Chichester, West Sussex, England : , : Wiley-Blackwell, , 2017 |
Descrizione fisica | 1 online resource (427 pages) |
Disciplina | 571.6/38 |
Soggetto topico |
Cell culture
Cytology - Technique |
ISBN |
1-118-85163-3
1-118-85153-6 1-118-85164-1 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNINA-9910271041603321 |
Chichester, West Sussex, England : , : Wiley-Blackwell, , 2017 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Technology platforms for 3D cell culture : a user's guide / / edited by Stefan Przyborski |
Pubbl/distr/stampa | Chichester, West Sussex : , : Wiley-Blackwell, , [2017] |
Descrizione fisica | 1 online resource (427 pages) |
Disciplina | 571.6/38 |
Soggetto topico |
Cell culture
Cytology - Technique |
ISBN |
1-118-85163-3
1-118-85153-6 1-118-85164-1 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNINA-9910831032803321 |
Chichester, West Sussex : , : Wiley-Blackwell, , [2017] | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|