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Development of three-dimensional cell cultures in film-based microdevices / / von Pavel Mitkov Nikolov
| Development of three-dimensional cell cultures in film-based microdevices / / von Pavel Mitkov Nikolov |
| Autore | Nikolov Pavel Mitkov |
| Pubbl/distr/stampa | Göttingen, Germany : , : Cuvillier Verlag, , 2015 |
| Descrizione fisica | 1 online resource (232 pages) : illustrations |
| Disciplina | 571.6028 |
| Soggetto topico | Cytology - Technique |
| Soggetto genere / forma | Electronic books. |
| ISBN | 3-7369-8063-9 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910511733203321 |
Nikolov Pavel Mitkov
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| Göttingen, Germany : , : Cuvillier Verlag, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Development of three-dimensional cell cultures in film-based microdevices / / von Pavel Mitkov Nikolov
| Development of three-dimensional cell cultures in film-based microdevices / / von Pavel Mitkov Nikolov |
| Autore | Nikolov Pavel Mitkov |
| Pubbl/distr/stampa | Göttingen, Germany : , : Cuvillier Verlag, , 2015 |
| Descrizione fisica | 1 online resource (232 pages) : illustrations |
| Disciplina | 571.6028 |
| Soggetto topico | Cytology - Technique |
| ISBN | 3-7369-8063-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910794948803321 |
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Nikolov Pavel Mitkov
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| Göttingen, Germany : , : Cuvillier Verlag, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Development of three-dimensional cell cultures in film-based microdevices / / von Pavel Mitkov Nikolov
| Development of three-dimensional cell cultures in film-based microdevices / / von Pavel Mitkov Nikolov |
| Autore | Nikolov Pavel Mitkov |
| Pubbl/distr/stampa | Göttingen, Germany : , : Cuvillier Verlag, , 2015 |
| Descrizione fisica | 1 online resource (232 pages) : illustrations |
| Disciplina | 571.6028 |
| Soggetto topico | Cytology - Technique |
| ISBN | 3-7369-8063-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910821372903321 |
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Nikolov Pavel Mitkov
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| Göttingen, Germany : , : Cuvillier Verlag, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Effusion cytology [[electronic resource] ] : a practical guide to cancer diagnosis / / Parvin Ganjei-Azar, Mercè Jordà, Awtar Krishan
| Effusion cytology [[electronic resource] ] : a practical guide to cancer diagnosis / / Parvin Ganjei-Azar, Mercè Jordà, Awtar Krishan |
| Autore | Ganjei-Azar Parvin |
| Pubbl/distr/stampa | New York, : Demos Medical, c2011 |
| Descrizione fisica | 1 online resource (192 p.) |
| Disciplina | 616.07/582 |
| Altri autori (Persone) |
JordàMercè
KrishanAwtar |
| Soggetto topico |
Cytodiagnosis
Cancer - Diagnosis Body fluids Cytology - Technique |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-283-31757-5
9786613317575 1-935281-41-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Body-cavity fluid cytology -- General diagnostic criteria: benign versus malignant -- Types of malignancy based on conventional cytology and immunocytochemistry -- Major differential diagnoses -- Detection of the primary site of carcinomas -- Cerebrospinal fluid cytology -- Laboratory techniques -- Flow cytometry, immunoassays, and molecular techniques. |
| Record Nr. | UNINA-9910457443803321 |
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Ganjei-Azar Parvin
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| New York, : Demos Medical, c2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Effusion cytology [[electronic resource] ] : a practical guide to cancer diagnosis / / Parvin Ganjei-Azar, Mercè Jordà, Awtar Krishan
| Effusion cytology [[electronic resource] ] : a practical guide to cancer diagnosis / / Parvin Ganjei-Azar, Mercè Jordà, Awtar Krishan |
| Autore | Ganjei-Azar Parvin |
| Pubbl/distr/stampa | New York, : Demos Medical, c2011 |
| Descrizione fisica | 1 online resource (192 p.) |
| Disciplina | 616.07/582 |
| Altri autori (Persone) |
JordàMercè
KrishanAwtar |
| Soggetto topico |
Cytodiagnosis
Cancer - Diagnosis Body fluids Cytology - Technique |
| ISBN |
1-283-31757-5
9786613317575 1-935281-41-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Body-cavity fluid cytology -- General diagnostic criteria: benign versus malignant -- Types of malignancy based on conventional cytology and immunocytochemistry -- Major differential diagnoses -- Detection of the primary site of carcinomas -- Cerebrospinal fluid cytology -- Laboratory techniques -- Flow cytometry, immunoassays, and molecular techniques. |
| Record Nr. | UNINA-9910781597603321 |
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Ganjei-Azar Parvin
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| New York, : Demos Medical, c2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Effusion cytology [[electronic resource] ] : a practical guide to cancer diagnosis / / Parvin Ganjei-Azar, Mercè Jordà, Awtar Krishan
| Effusion cytology [[electronic resource] ] : a practical guide to cancer diagnosis / / Parvin Ganjei-Azar, Mercè Jordà, Awtar Krishan |
| Autore | Ganjei-Azar Parvin |
| Pubbl/distr/stampa | New York, : Demos Medical, c2011 |
| Descrizione fisica | 1 online resource (192 p.) |
| Disciplina | 616.07/582 |
| Altri autori (Persone) |
JordàMercè
KrishanAwtar |
| Soggetto topico |
Cytodiagnosis
Cancer - Diagnosis Body fluids Cytology - Technique |
| ISBN |
1-283-31757-5
9786613317575 1-935281-41-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Body-cavity fluid cytology -- General diagnostic criteria: benign versus malignant -- Types of malignancy based on conventional cytology and immunocytochemistry -- Major differential diagnoses -- Detection of the primary site of carcinomas -- Cerebrospinal fluid cytology -- Laboratory techniques -- Flow cytometry, immunoassays, and molecular techniques. |
| Record Nr. | UNINA-9910822448003321 |
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Ganjei-Azar Parvin
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| New York, : Demos Medical, c2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Fluorescent materials for cell imaging / / Fu-gen Wu, editor
| Fluorescent materials for cell imaging / / Fu-gen Wu, editor |
| Edizione | [1st ed. 2020.] |
| Pubbl/distr/stampa | Gateway East, Singapore : , : Springer, , [2020] |
| Descrizione fisica | 1 online resource (V, 247 p. 140 illus., 128 illus. in color.) |
| Disciplina | 571.6028 |
| Soggetto topico | Cytology - Technique |
| ISBN | 981-15-5062-X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Chapter 1: Introduction: Fluorescent materials for cell imaging -- Chapter 2: Semiconductor quantum dots for cell imaging -- Chapter 3: Carbon nanodots for cell imaging -- Chapter 4: Silicon nanoparticles for cell imaging -- Chapter 5: Fluorescent metal nanoclusters for bioimaging -- Chapter 6: Lanthanide-based upconversion nanoparticles for bioimaging applications -- Chapter 7: Conjugated polymers and polymer dots for cell imaging -- Chapter 8: Aggregation-induced emission (AIE) probes for cell imaging -- Chapter 9: Luminescent coordination compounds for cell imaging. |
| Record Nr. | UNINA-9910424637903321 |
| Gateway East, Singapore : , : Springer, , [2020] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Fluorescent probes / edited by G. S. Beddard, M. A. West
| Fluorescent probes / edited by G. S. Beddard, M. A. West |
| Autore | Beddard, G. S. |
| Pubbl/distr/stampa | London : Academic Press, 1981 |
| Descrizione fisica | x, 235 p. : ill. ; 24 cm. |
| Disciplina | 571.64 |
| Altri autori (Persone) | West, Michael A. |
| Soggetto topico |
Biology - Technique
Cell membranes Cytology - Technique Fluorescent probes |
| ISBN | 0120846802 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISALENTO-991003475819707536 |
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Beddard, G. S.
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| London : Academic Press, 1981 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
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Handbook of single-cell technologies / / Tuhin Subhra Santra, Fan-Gang Tseng, editors
| Handbook of single-cell technologies / / Tuhin Subhra Santra, Fan-Gang Tseng, editors |
| Pubbl/distr/stampa | Singapore : , : Springer, , [2022] |
| Descrizione fisica | 1 online resource (1091 pages) |
| Disciplina | 571.6028 |
| Soggetto topico |
Cytology - Technique
Cells - Analysis |
| ISBN | 981-10-8953-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Intro -- Preface -- Contents -- About the Editors -- Contributors -- Part I: Physical Methods for Single Cell Therapy and Analysis -- 1 Light-Induced Cellular Delivery and Analysis -- Introduction -- Light-Induced Intracellular Delivery from Bulk to Single-Cell Photoporation -- Direct Laser-Cell Interaction -- Indirect Laser-Cell Interaction -- Pulsed Laser Systems for Optoporation -- Nanosecond Laser -- Femtosecond Laser -- Modifications of the Method -- Applications of Single-Cell Surgery -- Conclusion -- References -- 2 Mechanoporation: Toward Single Cell Approaches -- Introduction -- Types of Mechanoporation -- Particle Bombardment -- Microinjection -- Nanoneedle AFM Tip -- Microinjection Using Microfluidic Platforms -- Parallel Delivery -- Oscillating Nanoneedle Array -- Pressure Driven Devices -- Hydrodynamic Effect -- Sonoporation -- Shear Flow Devices -- A Cone and Plate Shearing Device -- Microchannel-Based Devices -- Mechanical Confinement -- High-Throughput Parallel Mechanical Confinement Device -- Microfluidic Devices -- Constriction Channel-Based Delivery -- Cell Squeeze Platform -- DNA Transfection Via Mechanical and Electrical Cell Membrane Disruption -- Advantages and Limitations of Mechanoporation -- Future Prospects -- Summary -- References -- 3 Single-Cell Electroporation -- Introduction -- Brief History -- Operation Mechanism -- Challenges in Macroscale Electroporation -- Landscape Transformation -- Electroporation Theories -- Intracellular Molecular Transport -- Cell Membrane Resealing Dynamics -- Microscale Electroporation -- Patch-Clamp Based Techniques -- Patch-Free Techniques -- Feedback-Driven Approaches -- Conclusion and Future Outlook -- References -- 4 Microinjection for Single-Cell Analysis and Therapy -- Introduction -- Development of Microinjection -- History of Microinjection.
Types of Cargos Used for Microinjection -- Types of Host Cells Used in Microinjection -- Instruments for Microinjection -- Microinjection for Single-Cell Analysis -- Injection for Analysis of Changes in Cells -- Transgenic Animal Production -- Modern Advancements for Increasing Efficiency -- Microinjection for Single-Cell Therapy -- Intracytoplasmic Sperm Injection for Treatment of Infertility -- Gene Therapy by Injection -- Microneedles for Therapeutic Uses -- Advantages -- Disadvantages -- Future Perspective and Conclusion -- References -- Part II: Fluidic System and Integration -- 5 Single-Cell Manipulation -- Introduction -- Flow Modeling of Hydrodynamic Cell Manipulation -- Fluidic Resistance Versus Electric Resistance -- Conservation of Mass and Energy Versus Kirchhoff´s Current Law and Voltage Law -- Division of Pressure and Flow Rate Versus Division of Voltage and Current -- Examples of Microfluidic Manipulation -- Single-Cell Trapping -- Co-culture of Single Cells -- Deforming, Sorting, and Separating Single Cells -- Integration with Other Methods -- Optical Integration -- Dielectrophoretic Integration -- Magnetic Integration -- Acoustic Integration -- Conclusion -- References -- 6 Single Cell Manipulation Using Macro-scale Actuator -- Introduction -- Transfer Function of Macro-to-Micro Manipulation (Mizoue et al. 2017) -- The Driving Mechanism -- Mechanical Model -- System Identification Through Experiments -- On-Chip Transmitter for Enhancing Manipulation Speed (Monzawa et al. 2015) -- Advantages of Using an Actuation Transmitter -- Mechanical Model and Theoretical Basis -- Experimental Validation on Manipulation with an Actuation Transmitter -- Recent Works and Applications -- Summary -- References -- 7 Inertial Microfluidics for Single-Cell Manipulation and Analysis -- Introduction -- Underlying Physics -- Inertial Migration. Dean Flow -- Viscoelasticity -- Guidelines for Designing Spiral Inertial Microfluidics -- Dimensionless Numbers -- Design Guidelines -- Cross-Sectional Dimensions -- Channel Length (Loop Number) -- Other Structural Parameters -- Other Functional Units -- Operational Parameters -- Improved Understandings on Spiral Inertial Microfluidics -- Application Guidelines for Spiral Inertial Microfluidics -- Focusing/Ordering -- Separation -- Concentration/Microfiltration -- Conclusion and Future Perspective -- References -- 8 Digital Microfluidics for Single Cell Manipulation and Analysis -- Introduction -- DMF Systems -- DMF Technology -- Electrowetting-on-Dielectric -- Electrodewetting -- Liquid Dielectrophoresis -- Dielectrophoresis -- Optoelectrowetting -- Optoelectronic Tweezers -- Magnetic Force -- Fabrication of DMF -- DMF Manipulation and Analysis of a Single Cell -- Adherent Cell -- Suspension Cell -- Long-Term Culture -- Related Applications of DMF -- Cell Sorting and Concentrating -- 3D Cell Culture -- Diagnosis and Clinical Application -- Conclusions and Future Outlook -- References -- 9 Single-Cell Separation -- Introduction -- Conventional Cell Separation Techniques -- Centrifugation -- Fluorescence-Activated Cell Sorting -- Magnetic-Activated Cell Sorting -- Laser Capture Microdissection -- Manual Cell Picking -- Microfluidic Single-Cell Separation Techniques -- Microfluidic Passive Separation Techniques -- Filter-Based Separation -- Deterministic Lateral Displacement -- Hydrodynamic Separation -- Non-inertial Hydrodynamic Separation -- Inertial Hydrodynamic Separation -- Microfluidic Active Separation Techniques -- Dielectrophoresis (DEP) -- Magnetic Separation -- Acoustophoresis -- Affinity-Based Separation -- Comparison Between Different Microfluidic Separation Techniques -- Conclusion -- References. 10 Technologies for Automated Single Cell Isolation -- Introduction -- Cell Samples -- Size and Morphology of Cells -- Sample Preparation: Single-Cell Suspensions -- Basic Considerations, Definitions, and Classifications for Single-Cell Isolation -- Automated Single-Cell Isolation Technologies -- Limiting Dilution -- Suitability for Microbial Cells -- Fluorescence-Activated Cell Sorting -- Suitability for Microbial Cells -- Single-Cell Dispensing (SCD) -- Suitability for Microbial Cells -- Microfluidic Single-Cell Isolation -- Hydrodynamic Trapping -- Vertical Trapping in Nanowells -- Suitability for Microbial Cells -- Droplet Microfluidics -- Single-Cell Manipulation (on Microfluidic Chips) by Directed External Forces -- Optical Tweezers (OT) -- Dielectrophoresis (DEP) -- Suitability for Microbial Cells -- Automated Micromanipulators -- Discussion and Conclusion -- References -- 11 Dual-Well Microfluidic Technique for Single Cell Isolation and Long-Term Clonal Culture -- Introduction -- Applications of Monoclonal Cell Culture -- Overview of Single Cell Isolation Methods for Clonal Culture -- The Design Concept of the Dual-Well Technique -- Fabrication of Dual-Well Device -- Fabrication of Master Molds by Photolithography Technology -- Molding PDMS Device with the Master Molds -- Dual-Well Device Preparation for Single Cell Isolation -- Cell Suspension Preparation for Single Cell Isolation with Dual-Well Device -- Single Cell Isolation and Clonal Culture with Dual-Well Device -- Culture Medium Replacement of the Dual-Well Device -- Discussion -- The Effect of Capture Well Depth on Single Cell Efficiency -- The Effect of Washing Flow Rate on Single Cell Efficiency -- The Effect of Cell Type on Single Cell Capture Efficiency -- The Effect of Device Flipping on Cell Transfer Efficiency. Single Cell-Derived Clonal Colonies and Stem Cell Differentiation in the Microwells of the Dual-Well Device -- Conclusion -- References -- 12 Single-Cell Cultivation Utilizing Microfluidic Systems -- Introduction -- Purpose of Single-Cell Cultivation -- Morphology -- Proliferation and Differentiation -- Migration -- Genomics, Transcriptomics, Proteomics, and Metabolomics -- Epigenomics -- Single-Cell Cultivation in Microfluidic Devices -- Cell Coculture -- Neural Coculture -- Coculture of Tumor Cells to Neighboring Cells -- Coculture of Neurons and Cancer Cells -- Molecule-Induced Cellular Behaviors -- Molecular Cue-Guided Neuron -- Molecule and Immune Cell Interaction -- Molecule and Microbe Interaction -- Regeneration -- Axotomy -- Stem Cell-Based Regeneration -- Further Limitations and Future Prospects -- Conclusion -- References -- 13 Integrated Microwell Array Technologies for Single Cell Analysis -- Introduction -- Microfabrication of a Microwell Array -- Material and Design Considerations -- Soft Photolithography for Fast Prototyping of Microwell Arrays -- Hydrophilic-in-Hydrophobic Microwells -- Polyethylene Glycol Microwells for Reduced Non-specific Adsorption -- Single Cell Docking -- Docking Strategies: Manual Administration of Cells -- Docking Strategies: Microfluidics-Assisted Administration of Cells -- Continuous Flow Microfluidics -- Digital Microfluidics -- Single Cell Manipulation -- Optical Manipulation -- Magnetic Manipulation -- Mechanical Manipulation -- Electric Manipulation -- Applications -- Single Cell Drug Screening -- Single Cell Omics -- Detection of Single Cell Secreted Products -- Other Applications -- Conclusion -- References -- 14 Micro- and Nanopore Technologies for Single-Cell Analysis -- Coulter Principle -- A History Behind the Invention of Coulter Principle -- Single-Cell Counting Mechanism. Single-Cell Analysis with Conventional Coulter Counters. |
| Record Nr. | UNINA-9910508470903321 |
| Singapore : , : Springer, , [2022] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Methods in bioengineering : systems analysis of biological networks / / Arul Jayaraman, Juergen Hahn, editors
| Methods in bioengineering : systems analysis of biological networks / / Arul Jayaraman, Juergen Hahn, editors |
| Pubbl/distr/stampa | Boston [Mass.] : , : Artech House, , ©2009 |
| Descrizione fisica | 1 online resource (328 p.) |
| Disciplina |
610.28
660.6 |
| Altri autori (Persone) |
JayaramanArul
HahnJuergen |
| Collana | Artech House methods in bioengineering series |
| Soggetto topico |
Bacterial genetics
Cytology - Technique Viral genetics |
| Soggetto genere / forma | Electronic books. |
| ISBN | 1-59693-407-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Methods in Bioengineering: Systems Analysis of Biological Networks; Contents; Chapter 1 Quantitative Immunofluorescence for Measuring Spatial Compartmentation of Covalently Modified Signaling Proteins; 1.1 Introduction; 1.2 Experimental Design; 1.3 Materials; 1.3.1 Cell culture; 1.3.2 Buffers/reagents; 1.3.3 Immunofluorescence reagents; 1.4 Methods; 1.4.1 Cell culture and stimulation for phospho-ERK measurements; 1.4.2 Antibody labeling of phosphorylated ERK (ppERK); 1.4.3 Fluorescence microscopy imaging of ppERK and automated imageanalysis
1.5 Data Acquisition, Anticipated Results, and Interpretation1.6 Statistical Guidelines; 1.7 Discussion and Commentary; 1.8 Application Notes; 1.9 Summary Points; Acknowledgments; References; Chapter 2 Development of Green Fluorescent Protein-Based Reporter Cell Lines for Dynamic Profiling of Transcription Factor and Kinase Activation; 2.1 Introduction; 2.2 Materials; 2.2.1 Cell and bacterial culture; 2.2.2 Buffers and reagents; 2.2.3 Cloning; 2.2.4 Microscopy; 2.3 Methods; 2.3.1 3T3-L1 cell culture; 2.3.2 Transcription factor reporter development; 2.3.3 Kinase reporter development 2.4 Application Notes2.4.1 Electroporation of TF reporter plasmids into 3T3-L1 preadipocytes; 2.4.2 Monitoring activation of ERK in HepG2 cells; 2.5 Data Acquisition, Anticipated Results, and Interpretation; 2.6 Discussion and Commentary; 2.7 Summary Points; Acknowledgments; References; Chapter 3 Comparison of Algorithms for Analyzing Fluorescent Microscopy Images and Computation of Transcription Factor Profiles; 3.1 Introduction; 3.2 Preliminaries; 3.2.1 Principles of GFP reporter systems; 3.2.2 Wavelets; 3.2.3 K-means clustering; 3.2.4 Principal component analysis 3.2.5 Mathematical description of digital images and image analysis3.3 Methods; 3.3.1 Image analysis based on wavelets and a bidirectional search; 3.3.2 Image analysis based on K-means clustering and PCA; 3.3.3 Determining fluorescence intensity of an image; 3.3.4 Comparison of the two image analysis procedures; 3.4 Data Acquisition, Anticipated Results, and Interpretation; 3.4.1 Developing a model describing the relationship between the transcription factor concentration and the observed fluorescence intensity 3.4.2 Solution of an inverse problem for determining transcription factor concentrations3.5 Application Notes; 3.6 Summary and Conclusions; Acknowledgments; References; Chapter 4 Data-Driven, Mechanistic Modeling of Biochemical Reaction Networks; 4.1 Introduction; 4.2 Principles of Data-Driven Modeling; 4.2.1 Types of experimental data; 4.2.2 Data processing and normalization; 4.2.3 Suitability of models used in conjunction with quantitative data; 4.2.4 Issues related to parameter specification and estimation; 4.3 Examples of Data-Driven Modeling 4.3.1 Example 1: Systematic analysis of crosstalk in the PDGF receptor signaling network |
| Altri titoli varianti | Systems analysis of biological networks |
| Record Nr. | UNINA-9910456668503321 |
| Boston [Mass.] : , : Artech House, , ©2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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