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Plant-bacteria interactions : strategies and techniques to promote plant growth / / edited by Iqbal Ahmad, John Pichtel, and Shamsul Hayat
Plant-bacteria interactions : strategies and techniques to promote plant growth / / edited by Iqbal Ahmad, John Pichtel, and Shamsul Hayat
Pubbl/distr/stampa Weinheim, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2008
Descrizione fisica 1 online resource (330 p.)
Disciplina 571.82
Soggetto topico Growth (Plants)
Plant growth promoting substances
Plant biotechnology
ISBN 1-281-94671-0
9786611946715
3-527-62198-9
3-527-62199-7
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Plant-Bacteria Interactions; Contents; List of Contributors; 1 Ecology, Genetic Diversity and Screening Strategies of Plant Growth Promoting Rhizobacteria (PGPR); 1.1 Introduction; 1.1.1 Rhizosphere Microbial Ecology; 1.1.2 Plant Growth Promoting Rhizobacteria (PGPR); 1.2 Rhizosphere Microbial Structure; 1.2.1 Methods to Study the Microbial Structure in the Rhizosphere; 1.2.2 Ecology and Biodiversity of PGPR Living in the Rhizosphere; 1.2.2.1 Diazotrophic PGPR; 1.2.2.2 Bacillus; 1.2.2.3 Pseudomonas; 1.2.2.4 Rhizobia; 1.3 Microbial Activity and Functional Diversity in the Rhizosphere
1.3.1 Methods to Study Activity and Functional Diversity in the Rhizosphere1.3.2 Activity and Effect of PGPR in the Rhizosphere; 1.4 Screening Strategies of PGPR; 1.5 Conclusions; 1.6 Prospects; References; 2 Physicochemical Approaches to Studying Plant Growth Promoting Rhizobacteria; 2.1 Introduction; 2.2 Application of Vibrational Spectroscopy to Studying Whole Bacterial Cells; 2.2.1 Methodological Background; 2.2.2 Vibrational Spectroscopic Studies of A. brasilense Cells; 2.2.2.1 Effects of Heavy Metal Stress on A. brasilense Metabolism
2.2.2.2 Differences in Heavy Metal Induced Metabolic Responses in Epiphytic and Endophytic A. brasilense Strains2.3 Application of Nuclear γ-Resonance Spectroscopy to Studying Whole Bacterial Cells; 2.3.1 Methodological Background; 2.3.2 Emission Mössbauer Spectroscopic Studies of Cobalt(II) Binding and Transformations in A. brasilense Cells; 2.4 Structural Studies of Glutamine Synthetase (GS) from A. brasilense; 2.4.1 General Characterization of the Enzyme; 2.4.2 Circular Dichroism Spectroscopic Studies of the Enzyme Secondary Structure
2.4.2.1 Methodology of Circular Dichroism (CD) Spectroscopic Analysis of Protein Secondary Structure2.4.2.2 The Effect of Divalent Cations on the Secondary Structure of GS from A. brasilense; 2.4.3 Emission Mössbauer Spectroscopic Analysis of the Structural Organization of the Cation-Binding Sites in the Enzyme Active Centers; 2.4.3.1 Methodological Outlines and Prerequisites; 2.4.3.2 Experimental Studies of A. brasilense GS; 2.4.3.3 Conclusions and Outlook; 2.5 General Conclusions and Future Directions of Research; References
3 Physiological and Molecular Mechanisms of Plant Growth Promoting Rhizobacteria (PGPR)3.1 Introduction; 3.2 PGPR Grouped According to Action Mechanisms; 3.2.1 PGPR Using Indirect Mechanisms; 3.2.1.1 Free Nitrogen-Fixing PGPR; 3.2.1.2 Siderophore-Producing PGPR; 3.2.1.3 Phosphate-Solubilizing PGPR; 3.2.2 PGPR Using Direct Mechanisms; 3.2.2.1 PGPR that Modify Plant Growth Regulator Levels; 3.2.2.2 PGPR that Induce Systemic Resistance; 3.3 Conclusions; 3.4 Future Prospects; References; 4 A Review on the Taxonomy and Possible Screening Traits of Plant Growth Promoting Rhizobacteria
4.1 Introduction
Record Nr. UNINA-9910677023603321
Weinheim, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2008
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Polycyclic arenes and heteroarenes : synthesis, properties, and applications / / edited by Qian Miao
Polycyclic arenes and heteroarenes : synthesis, properties, and applications / / edited by Qian Miao
Pubbl/distr/stampa Weinheim an der Bergstrasse, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2016
Descrizione fisica 1 online resource (417 pages) : illustrations
Disciplina 547.61
Soggetto topico Polycyclic aromatic hydrocarbons
Soggetto genere / forma Electronic books.
ISBN 3-527-68957-5
3-527-68954-0
3-527-68955-9
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910131613903321
Weinheim an der Bergstrasse, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2016
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Polycyclic arenes and heteroarenes : synthesis, properties, and applications / / edited by Qian Miao
Polycyclic arenes and heteroarenes : synthesis, properties, and applications / / edited by Qian Miao
Pubbl/distr/stampa Weinheim an der Bergstrasse, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2016
Descrizione fisica 1 online resource (417 pages) : illustrations
Disciplina 547.61
Soggetto topico Polycyclic aromatic hydrocarbons
ISBN 3-527-68957-5
3-527-68954-0
3-527-68955-9
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910830511903321
Weinheim an der Bergstrasse, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2016
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Proceedings of the Galactic Center Workshop 2002 : the central 300 parsecs of the Milky Way / / A. Cotera [and three others], (eds.)
Proceedings of the Galactic Center Workshop 2002 : the central 300 parsecs of the Milky Way / / A. Cotera [and three others], (eds.)
Pubbl/distr/stampa Weinheim, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2004
Descrizione fisica 1 online resource (653 p.)
Disciplina 523.113
Collana Astronomische Nachrichten, Supplementary Issue
Soggetto topico Galaxies
Soggetto genere / forma Electronic books.
ISBN 1-281-84319-9
9786611843199
3-527-61798-1
3-527-61799-X
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Galactic Center Workshop 2002; Contents; Chapter 1: Large Scale Structures: Surveys and Interactions; Chapter 2: Molecular Clouds and Magnetic Fields; Chapter 3: Sagittarius A and its Environs; Chapter 4: Stars and Star Formation; Chapter 5: Sgr A* I: New Observational Results; Chapter 6: Sgr A* 11: Theoretical Models; Chapter 7: The Central Parsec; Chapter 8: Morphology and Dynamics of the Central 10 Parsecs
Record Nr. UNINA-9910144721203321
Weinheim, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2004
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Proceedings of the Galactic Center Workshop 2002 : the central 300 parsecs of the Milky Way / / A. Cotera [and three others], (eds.)
Proceedings of the Galactic Center Workshop 2002 : the central 300 parsecs of the Milky Way / / A. Cotera [and three others], (eds.)
Pubbl/distr/stampa Weinheim, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2004
Descrizione fisica 1 online resource (653 p.)
Disciplina 523.113
Collana Astronomische Nachrichten, Supplementary Issue
Soggetto topico Galaxies
ISBN 1-281-84319-9
9786611843199
3-527-61798-1
3-527-61799-X
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Galactic Center Workshop 2002; Contents; Chapter 1: Large Scale Structures: Surveys and Interactions; Chapter 2: Molecular Clouds and Magnetic Fields; Chapter 3: Sagittarius A and its Environs; Chapter 4: Stars and Star Formation; Chapter 5: Sgr A* I: New Observational Results; Chapter 6: Sgr A* 11: Theoretical Models; Chapter 7: The Central Parsec; Chapter 8: Morphology and Dynamics of the Central 10 Parsecs
Record Nr. UNINA-9910831162503321
Weinheim, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2004
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Protein degradation . Vol. 4 The ubiquitin-proteasome system and disease / / edited by R. John Mayer, Aaron Ciechanover, Martin Rechsteiner
Protein degradation . Vol. 4 The ubiquitin-proteasome system and disease / / edited by R. John Mayer, Aaron Ciechanover, Martin Rechsteiner
Pubbl/distr/stampa Weinheim, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2008
Descrizione fisica 1 online resource (260 p.)
Disciplina 572.76
612.3/98
Collana Protein Degradation
Soggetto topico Proteins - Metabolism
Ubiquitin
Soggetto genere / forma Electronic books.
ISBN 1-283-14037-3
9786613140371
3-527-62023-0
3-527-62030-3
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Protein Degradation; Contents; Preface; List of Contributors; 1 Ubiquitin Signaling and Cancer Pathogenesis; 1.1 Introduction; 1.1.1 Ubiquitin Signaling Networks; 1.1.2 Ubiquitin-like Proteins; 1.2 Ubiquitin in Cancer Pathogenesis; 1.2.1 Ubiquitin in Cell Cycle Control; 1.2.2 Ubiquitin in the NF-κB Pathway; 1.2.3 Ubiquitin as a Signal in DNA Repair; 1.2.3.1 p53 Pathway; 1.2.3.2 BRCA1 and FANCD2; 1.2.3.3 PCNA and TLS Polymerases; 1.2.4 Ubiquitin Networks in Angiogenesis; 1.2.5 Ubiquitin Networks in Receptor Endocytosis; 1.3 Targeting Ubiquitin Networks in Cancers
1.3.1 Targeting Interactions between E3s and their Substrates1.3.2 Targeting the Proteasome; 1.3.3 Other Approaches; 1.4 Conclusions and Future Perspectives; 2 Regulation of the p53 Tumor-suppressor Protein by Ubiquitin and Ubiquitin-like Molecules; 2.1 Functional Domains of p53; 2.2 The Family of Ubiquitin-like Molecules; 2.3 E3 Ligases for p53; 2.4 Modification of p53 with Ubiquitin; 2.5 Requirements for Mdm2-mediated Ubiquitination of p53; 2.6 Regulation of p53 Ubiquitination; 2.6.1 E2 Conjugating Enzymes; 2.6.2 Interacting Proteins; 2.6.3 By Other Post-translational Modifications
2.7 De-ubiquitination of p532.8 SUMO-1/sentrin/smpt3; 2.9 NEDD8/Rub1; 2.10 Therapeutic Intervention through the Ubiquitin Pathway; 3 The Ubiquitin-Proteasome System in Epstein-Barr Virus Infection and Oncogenesis; 3.1 Introduction; 3.2 Viral Interference with the Ubiquitin-Proteasome System; 3.3 The EBV Life Cycle; 3.4 EBV and the Ubiquitin-Proteasome System; 3.4.1 EBNA1; 3.4.2 EBNA6 (EBNA3C); 3.4.3 LMP1; 3.4.4 LMP2; 3.4.5 BZLF1 (Zta) and BRLF1 (Rta); 3.4.6 BPLF1; 3.5 EBV-associated Malignancies; 3.6 Concluding Remarks; 4 HECT Ubiquitin-protein Ligases in Human Disease; 4.1 Introduction
4.2 Definition of HECT E3s4.3 Human HECT E3s and their Role in Disease; 4.4 E6-AP; 4.4.1 E6-AP and Cervical Cancer (Cancer of the Uterine Cervix); 4.4.2 E6-AP and Angelman Syndrome; 4.5 HECTH9; 4.6 HECT E3s with WW Domains; 4.6.1 Nedd4/Nedd4-2; 4.6.1.1 Nedd4/Nedd4-2 and Liddle's Syndrome; 4.6.1.2 Nedd4 and Retrovirus Budding; 4.6.2 Itch and the Immune Response; 4.6.3 Smurfs; 4.6.3.1 Smurfs and Cancer; 4.6.3.2 Smurfs and Bone Homeostasis; 4.7 Concluding Remarks; 5 Ubiquitin-independent Mechanisms of Substrate Recognition and Degradation by the Proteasome; 5.1 Introduction
5.2 Ubiquitin-independent Proteasome Substrates5.2.1 Ornithine Decarboxylase; 5.2.2 p21(Waf1/Cip1); 5.2.3 Retinoblastoma Protein; 5.2.4 p53 and p73; 5.2.5 Human Thymidylate Synthase; 5.2.6 Rpn4; 5.2.7 NF-κB and IκBα; 5.2.8 Steroid Receptor Co-activator-3; 5.2.9 c-Jun; 5.3 Mechanisms of Ubiquitin-independent Degradation; 5.4 Conclusion; 6 Endoplasmic Reticulum Protein Quality Control and Degradation; 6.1 Introduction; 6.2 ER-import, Folding and the Unfolded Protein Response; 6.3 General Principles and Components of ERQD (Endoplasmic Reticulum Quality Control and Protein Degradation)
6.4 Mechanism of ERQD
Record Nr. UNINA-9910144007703321
Weinheim, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2008
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Protein degradation . Vol. 4 The ubiquitin-proteasome system and disease / / edited by R. John Mayer, Aaron Ciechanover, Martin Rechsteiner
Protein degradation . Vol. 4 The ubiquitin-proteasome system and disease / / edited by R. John Mayer, Aaron Ciechanover, Martin Rechsteiner
Pubbl/distr/stampa Weinheim, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2008
Descrizione fisica 1 online resource (260 p.)
Disciplina 572.76
612.3/98
Collana Protein Degradation
Soggetto topico Proteins - Metabolism
Ubiquitin
ISBN 1-283-14037-3
9786613140371
3-527-62023-0
3-527-62030-3
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Protein Degradation; Contents; Preface; List of Contributors; 1 Ubiquitin Signaling and Cancer Pathogenesis; 1.1 Introduction; 1.1.1 Ubiquitin Signaling Networks; 1.1.2 Ubiquitin-like Proteins; 1.2 Ubiquitin in Cancer Pathogenesis; 1.2.1 Ubiquitin in Cell Cycle Control; 1.2.2 Ubiquitin in the NF-κB Pathway; 1.2.3 Ubiquitin as a Signal in DNA Repair; 1.2.3.1 p53 Pathway; 1.2.3.2 BRCA1 and FANCD2; 1.2.3.3 PCNA and TLS Polymerases; 1.2.4 Ubiquitin Networks in Angiogenesis; 1.2.5 Ubiquitin Networks in Receptor Endocytosis; 1.3 Targeting Ubiquitin Networks in Cancers
1.3.1 Targeting Interactions between E3s and their Substrates1.3.2 Targeting the Proteasome; 1.3.3 Other Approaches; 1.4 Conclusions and Future Perspectives; 2 Regulation of the p53 Tumor-suppressor Protein by Ubiquitin and Ubiquitin-like Molecules; 2.1 Functional Domains of p53; 2.2 The Family of Ubiquitin-like Molecules; 2.3 E3 Ligases for p53; 2.4 Modification of p53 with Ubiquitin; 2.5 Requirements for Mdm2-mediated Ubiquitination of p53; 2.6 Regulation of p53 Ubiquitination; 2.6.1 E2 Conjugating Enzymes; 2.6.2 Interacting Proteins; 2.6.3 By Other Post-translational Modifications
2.7 De-ubiquitination of p532.8 SUMO-1/sentrin/smpt3; 2.9 NEDD8/Rub1; 2.10 Therapeutic Intervention through the Ubiquitin Pathway; 3 The Ubiquitin-Proteasome System in Epstein-Barr Virus Infection and Oncogenesis; 3.1 Introduction; 3.2 Viral Interference with the Ubiquitin-Proteasome System; 3.3 The EBV Life Cycle; 3.4 EBV and the Ubiquitin-Proteasome System; 3.4.1 EBNA1; 3.4.2 EBNA6 (EBNA3C); 3.4.3 LMP1; 3.4.4 LMP2; 3.4.5 BZLF1 (Zta) and BRLF1 (Rta); 3.4.6 BPLF1; 3.5 EBV-associated Malignancies; 3.6 Concluding Remarks; 4 HECT Ubiquitin-protein Ligases in Human Disease; 4.1 Introduction
4.2 Definition of HECT E3s4.3 Human HECT E3s and their Role in Disease; 4.4 E6-AP; 4.4.1 E6-AP and Cervical Cancer (Cancer of the Uterine Cervix); 4.4.2 E6-AP and Angelman Syndrome; 4.5 HECTH9; 4.6 HECT E3s with WW Domains; 4.6.1 Nedd4/Nedd4-2; 4.6.1.1 Nedd4/Nedd4-2 and Liddle's Syndrome; 4.6.1.2 Nedd4 and Retrovirus Budding; 4.6.2 Itch and the Immune Response; 4.6.3 Smurfs; 4.6.3.1 Smurfs and Cancer; 4.6.3.2 Smurfs and Bone Homeostasis; 4.7 Concluding Remarks; 5 Ubiquitin-independent Mechanisms of Substrate Recognition and Degradation by the Proteasome; 5.1 Introduction
5.2 Ubiquitin-independent Proteasome Substrates5.2.1 Ornithine Decarboxylase; 5.2.2 p21(Waf1/Cip1); 5.2.3 Retinoblastoma Protein; 5.2.4 p53 and p73; 5.2.5 Human Thymidylate Synthase; 5.2.6 Rpn4; 5.2.7 NF-κB and IκBα; 5.2.8 Steroid Receptor Co-activator-3; 5.2.9 c-Jun; 5.3 Mechanisms of Ubiquitin-independent Degradation; 5.4 Conclusion; 6 Endoplasmic Reticulum Protein Quality Control and Degradation; 6.1 Introduction; 6.2 ER-import, Folding and the Unfolded Protein Response; 6.3 General Principles and Components of ERQD (Endoplasmic Reticulum Quality Control and Protein Degradation)
6.4 Mechanism of ERQD
Record Nr. UNINA-9910830864303321
Weinheim, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2008
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Proteomics in practice : a guide to successful experimental design / / edited by Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker
Proteomics in practice : a guide to successful experimental design / / edited by Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker
Edizione [Second, completely revised edition.]
Pubbl/distr/stampa Weinheim, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2008
Descrizione fisica 1 online resource (504 p.)
Disciplina 572.6
572.64
Soggetto topico Proteomics
Proteins - Analysis
Molecular biology
Soggetto genere / forma Electronic books.
ISBN 1-281-94680-X
9786611946807
3-527-62229-2
3-527-62230-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Proteomics in Practice; Contents; Preface; Foreword; Abbreviations, Symbols, Units; Introduction; 1 History; 2 Critical Points; 2.1 Challenges of the Protein Samples; 2.2 Challenges of the Analysis Systems; 3 Proteomics Strategies; 3.1 Proteome Mapping; 3.2 Differential Analysis; 3.3 Time Point Experiments; 3.4 Verification of Targets or Biomarkers; 3.5 Integration of Results into Biological Context; 3.6 Systems Biology; 4 Concept of Experimental Planning; 4.1 Biological Replicates; 4.2 Pooling of Samples: Yes or No?; 4.3 Pre-fractionation of Samples: Yes or No?
4.4 Which is the Best Workflow to Start With?Part I: Proteomics Technology; 1 Electrophoretic Techniques; 1.1 The Principle of Electrophoresis and Some Methodological Background; 1.1.1 Free Flow Electrophoretic Methods; 1.1.2 Gels for Electrophoretic Techniques; 1.1.3 Electroendosmosis Effects; 1.2 Polyacrylamide Gel Electrophoresis; 1.2.1 The Polyacrylamide Gel; 1.2.2 SDS Polyacrylamide Gel Electrophoresis; 1.2.3 Blue Native Electrophoresis; 1.2.4 Cationic Detergent Electrophoresis; 1.3 Blotting; 1.3.1 Electrophoretic Transfer; 1.3.2 Protein Detection on the Membrane
1.4 Isoelectric Focusing1.4.1 Theoretical Background; 1.4.2 Preparation of IEF Gels; 1.4.3 Isoelectric Focusing in Proteomics; 1.5 Two-dimensional Electrophoresis; 1.5.1 Sample Preparation; 1.5.2 Pre-labeling of Proteins for Difference Gel Electrophoresis; 1.5.3 First Dimension: Isoelectric Focusing in IPG Strips; 1.5.4 Second Dimension: SDS Electrophoresis; 1.5.5 Detection of Protein Spots; 1.6 Image Analysis; 1.6.1 Image Acquisition; 1.6.2 Image Analysis and Evaluation; 1.6.3 Use of 2-D Electrophoresis Data; 1.7 Spot Handling; 1.7.1 Spot Picking; 1.7.2 Protein Cleavage
2 Liquid Chromatography Techniques2.1 Basic Principles of Important Liquid Chromatography Techniques; 2.1.1 Ion Exchange Chromatography; 2.1.2 Reversed Phase Chromatography; 2.1.3 Affinity Chromatography; 2.1.4 Gel Filtration; 2.2 Strategic Approach and General Applicability; 2.3 Liquid Chromatography Techniques and Applications in Proteome Analysis; 2.3.1 Peptide Separation; 2.3.2 2DLC Peptide Separation; 2.3.3 Affinity Chromatography and LC-MS/MS; 2.3.4 Protein Pre-fractionation; 2.4 Practical Considerations and Application of LC-based Protein Pre-fractionation
2.4.1 Sample Extraction and Preparation2.4.2 Experimental Setup; 2.4.3 Ion Exchange Chromatography and Protein Pre-fractionation; 2.4.4 Reversed Phase Chromatography and Protein Pre-fractionation; 2.4.5 Fraction Size and Number of Fractions; 2.5 Critical Review and Outlook; 3 Mass Spectrometry; 3.1 Ionization; 3.1.1 Matrix Assisted Laser Desorption Ionization; 3.1.2 Electrospray Ionization; 3.2 Ion Separation; 3.2.1 Time-of-Flight Analyzer; 3.2.2 Triple Quadrupole Analyzer; 3.2.3 Quadrupole Ion Trap; 3.2.4 Quadrupole Time-of-Flight; 3.2.5 Hybrid Triple Quadrupole Linear Ion Trap
3.2.6 TOF/TOF Analyzer
Record Nr. UNINA-9910144117403321
Weinheim, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2008
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Proteomics in practice : a guide to successful experimental design / / edited by Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker
Proteomics in practice : a guide to successful experimental design / / edited by Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker
Edizione [Second, completely revised edition.]
Pubbl/distr/stampa Weinheim, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2008
Descrizione fisica 1 online resource (504 p.)
Disciplina 572.6
572.64
Soggetto topico Proteomics
Proteins - Analysis
Molecular biology
ISBN 1-281-94680-X
9786611946807
3-527-62229-2
3-527-62230-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Proteomics in Practice; Contents; Preface; Foreword; Abbreviations, Symbols, Units; Introduction; 1 History; 2 Critical Points; 2.1 Challenges of the Protein Samples; 2.2 Challenges of the Analysis Systems; 3 Proteomics Strategies; 3.1 Proteome Mapping; 3.2 Differential Analysis; 3.3 Time Point Experiments; 3.4 Verification of Targets or Biomarkers; 3.5 Integration of Results into Biological Context; 3.6 Systems Biology; 4 Concept of Experimental Planning; 4.1 Biological Replicates; 4.2 Pooling of Samples: Yes or No?; 4.3 Pre-fractionation of Samples: Yes or No?
4.4 Which is the Best Workflow to Start With?Part I: Proteomics Technology; 1 Electrophoretic Techniques; 1.1 The Principle of Electrophoresis and Some Methodological Background; 1.1.1 Free Flow Electrophoretic Methods; 1.1.2 Gels for Electrophoretic Techniques; 1.1.3 Electroendosmosis Effects; 1.2 Polyacrylamide Gel Electrophoresis; 1.2.1 The Polyacrylamide Gel; 1.2.2 SDS Polyacrylamide Gel Electrophoresis; 1.2.3 Blue Native Electrophoresis; 1.2.4 Cationic Detergent Electrophoresis; 1.3 Blotting; 1.3.1 Electrophoretic Transfer; 1.3.2 Protein Detection on the Membrane
1.4 Isoelectric Focusing1.4.1 Theoretical Background; 1.4.2 Preparation of IEF Gels; 1.4.3 Isoelectric Focusing in Proteomics; 1.5 Two-dimensional Electrophoresis; 1.5.1 Sample Preparation; 1.5.2 Pre-labeling of Proteins for Difference Gel Electrophoresis; 1.5.3 First Dimension: Isoelectric Focusing in IPG Strips; 1.5.4 Second Dimension: SDS Electrophoresis; 1.5.5 Detection of Protein Spots; 1.6 Image Analysis; 1.6.1 Image Acquisition; 1.6.2 Image Analysis and Evaluation; 1.6.3 Use of 2-D Electrophoresis Data; 1.7 Spot Handling; 1.7.1 Spot Picking; 1.7.2 Protein Cleavage
2 Liquid Chromatography Techniques2.1 Basic Principles of Important Liquid Chromatography Techniques; 2.1.1 Ion Exchange Chromatography; 2.1.2 Reversed Phase Chromatography; 2.1.3 Affinity Chromatography; 2.1.4 Gel Filtration; 2.2 Strategic Approach and General Applicability; 2.3 Liquid Chromatography Techniques and Applications in Proteome Analysis; 2.3.1 Peptide Separation; 2.3.2 2DLC Peptide Separation; 2.3.3 Affinity Chromatography and LC-MS/MS; 2.3.4 Protein Pre-fractionation; 2.4 Practical Considerations and Application of LC-based Protein Pre-fractionation
2.4.1 Sample Extraction and Preparation2.4.2 Experimental Setup; 2.4.3 Ion Exchange Chromatography and Protein Pre-fractionation; 2.4.4 Reversed Phase Chromatography and Protein Pre-fractionation; 2.4.5 Fraction Size and Number of Fractions; 2.5 Critical Review and Outlook; 3 Mass Spectrometry; 3.1 Ionization; 3.1.1 Matrix Assisted Laser Desorption Ionization; 3.1.2 Electrospray Ionization; 3.2 Ion Separation; 3.2.1 Time-of-Flight Analyzer; 3.2.2 Triple Quadrupole Analyzer; 3.2.3 Quadrupole Ion Trap; 3.2.4 Quadrupole Time-of-Flight; 3.2.5 Hybrid Triple Quadrupole Linear Ion Trap
3.2.6 TOF/TOF Analyzer
Record Nr. UNINA-9910830618803321
Weinheim, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2008
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Skinology : the effect of TENCEL, fibers on human skin / / Lorenz Wied, Johannes Neuhofer, and Tom Burrow
Skinology : the effect of TENCEL, fibers on human skin / / Lorenz Wied, Johannes Neuhofer, and Tom Burrow
Autore Wied Lorenz
Pubbl/distr/stampa Weinheim, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2013
Descrizione fisica 1 online resource (138 pages) : illustrations (some color), tables, graphs
Disciplina 572.56682
Soggetto topico Cellulose fibers
ISBN 3-527-68129-9
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910153455403321
Wied Lorenz  
Weinheim, Germany : , : WILEY-VCH Verlag GmbH & Co. KGaA, , 2013
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui