In situ hybridization [[electronic resource] /] / Melody Clark |
Pubbl/distr/stampa | [Saddle River, NJ?, : Wiley-VCH, 2002?] |
Descrizione fisica | 1 online resource (182 p.) |
Disciplina |
572.84
574.87322 |
Altri autori (Persone) | ClarkMelody |
Collana | Laboratory companion |
Soggetto topico |
In situ hybridization
Genetics |
Soggetto genere / forma | Electronic books. |
ISBN |
1-281-75854-X
9786611758547 3-527-61507-5 3-527-61506-7 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
In Situ Hybridization; Contents; CHAPTER 1. Genomic In Situ Hybridization for Whole Chromosome and Genome Analysis; 1.1 Introduction; 1.2 Uses of Genomic In Situ Hybridization; 1.2.1 Genome Organization of Different Sequence Classes; 1.2.2 Species Identity, Differentiation and Relatedness; 1.2.3 Genomic Stability; 1.2.4 Spatial Organization of Chromosomes; 1.2.5 Chromosome lntrogression and Rearrangements; 1.2.6 Chromosome Behaviour and Meiosis; 1.3 The Principle of Genomic In Situ Hybridization; 1.3.1 Genomic Probing; 1.3.2 Blocking and Competitive Hybridization; 1.4 Materials and Chemicals
1.4.1 Buffers and Reagents1.4.2 Chemicals; 1.4.3 Equipment; 1.5 Genomic In Situ Hybridization: Protocol; 1.5.1 Chromosome Preparations; 1.5.2 Probe DNA; 1.5.3 Blocking DNA; 1.5.4 In Situ Hybridization Procedure; 1.6 Troubleshooting; CHAPTER 2. Fluorescence In Situ Hybridization: Applications in Gene Mapping and Clinical Diagnostics; 2.1 Introduction; 2.2 Materials and Chemicals; 2.2.1 Buffers and Reagents; 2.2.2 Chemicals; 2.2.3 Equipment; 2.3 FISH Using Single Copy Cosmid Probes; 2.3.1 Preparation of Metaphase Chromosomes; 2.3.2 Preparation of Metaphase Chromosome Slides for Hybridization 2.3.3 Preparation and Labelling of Probes2.3.4 Hybridization of Cosmid Probes; 2.4 Multicolor FISH Using Cosmid Probes; 2.4.1 Labelling of Probes and Hybridization Reactions; 2.5 lnterphase FISH Analysis; 2.5.1 Preparation of lnterphase Nuclear Slides from Short Term Cultures; 2.5.2 Direct Preparation of lnterphase Spreads; 2.5.3 FISH; 2.6 FISH Analysis Using YAC Clones; 2.6.1 Preparation of Metaphase Chromosomes; 2.6.2 Isolation of YAC DNA; 2.6.3 Biotin Labelling of YACs; 2.6.4 FISH; 2.6.5 Microscopy; 2.7 Direct Visual In Situ Hybridization (DIRVISH); 2.7.1 Preparation of Stretched DNA 2.7.2 FISH Hybridization2.7.3 Detection and Analysis; 2.7.4 Microscopy; 2.8 Troubleshooting; CHAPTER 3. Detection of Nucleic Acids (DNA and RNA) In Situ by Single and Cyclic Primed In Situ labelling (PRINS): Two Alternatives to Traditional In Situ Hybridization Methods; 3.1 Introduction; 3.2 Materials and Chemicals; 3.2.1 Buffers and Reagents; 3.2.2 Chemicals; 3.2.3 Equipment; 3.3 DNA-PRINS with Oligonucleotide Probes; 3.4 PRINS with Ddel Digested Cloned Probes; 3.4.1 Generation of Primers from Cloned Alpha Satellite DNA; 3.4.2 PRlNS Reaction; 3.5 Multicolour-PRINS; 3.6 PRINS-painting 3.7 PRINS-PCR and Repeated-PRINS of DNA3.7.1 Chromosome Spreads; 3.7.2 Pretreatment of Chromosome Spreads; 3.7.3 Labelling Reaction; 3.8 PRINS-PCR of mRNA; 3.9 Visualization of Hapten-Labelled Nucleotides; 3.9.1 Standard Slides; 3.9.1.1 Biotin; 3.9.1.2 Digoxigenin; 3.9.2 Micro-slides; 3.9.2.1 Detection of Labelled DNA; 3.9.2.2 Detection of Labelled RNA; 3.9.2.3 Alkaline Phosphatase Visualization; 3.9.2.4 Fluorescent Visualization; 3.10 Troubleshooting CHAPTER 4. Fluorescence lmmunophenotyping and lnterphase Cytogenetics as a Tool for Investigation of Neoplasms (FICTION): Combined In Situ Hybridization and Fluorescence lmmunophenotyping |
Record Nr. | UNINA-9910143989303321 |
[Saddle River, NJ?, : Wiley-VCH, 2002?] | ||
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Lo trovi qui: Univ. Federico II | ||
|
In situ hybridization [[electronic resource] /] / Melody Clark |
Pubbl/distr/stampa | [Saddle River, NJ?, : Wiley-VCH, 2002?] |
Descrizione fisica | 1 online resource (182 p.) |
Disciplina |
572.84
574.87322 |
Altri autori (Persone) | ClarkMelody |
Collana | Laboratory companion |
Soggetto topico |
In situ hybridization
Genetics |
ISBN |
1-281-75854-X
9786611758547 3-527-61507-5 3-527-61506-7 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
In Situ Hybridization; Contents; CHAPTER 1. Genomic In Situ Hybridization for Whole Chromosome and Genome Analysis; 1.1 Introduction; 1.2 Uses of Genomic In Situ Hybridization; 1.2.1 Genome Organization of Different Sequence Classes; 1.2.2 Species Identity, Differentiation and Relatedness; 1.2.3 Genomic Stability; 1.2.4 Spatial Organization of Chromosomes; 1.2.5 Chromosome lntrogression and Rearrangements; 1.2.6 Chromosome Behaviour and Meiosis; 1.3 The Principle of Genomic In Situ Hybridization; 1.3.1 Genomic Probing; 1.3.2 Blocking and Competitive Hybridization; 1.4 Materials and Chemicals
1.4.1 Buffers and Reagents1.4.2 Chemicals; 1.4.3 Equipment; 1.5 Genomic In Situ Hybridization: Protocol; 1.5.1 Chromosome Preparations; 1.5.2 Probe DNA; 1.5.3 Blocking DNA; 1.5.4 In Situ Hybridization Procedure; 1.6 Troubleshooting; CHAPTER 2. Fluorescence In Situ Hybridization: Applications in Gene Mapping and Clinical Diagnostics; 2.1 Introduction; 2.2 Materials and Chemicals; 2.2.1 Buffers and Reagents; 2.2.2 Chemicals; 2.2.3 Equipment; 2.3 FISH Using Single Copy Cosmid Probes; 2.3.1 Preparation of Metaphase Chromosomes; 2.3.2 Preparation of Metaphase Chromosome Slides for Hybridization 2.3.3 Preparation and Labelling of Probes2.3.4 Hybridization of Cosmid Probes; 2.4 Multicolor FISH Using Cosmid Probes; 2.4.1 Labelling of Probes and Hybridization Reactions; 2.5 lnterphase FISH Analysis; 2.5.1 Preparation of lnterphase Nuclear Slides from Short Term Cultures; 2.5.2 Direct Preparation of lnterphase Spreads; 2.5.3 FISH; 2.6 FISH Analysis Using YAC Clones; 2.6.1 Preparation of Metaphase Chromosomes; 2.6.2 Isolation of YAC DNA; 2.6.3 Biotin Labelling of YACs; 2.6.4 FISH; 2.6.5 Microscopy; 2.7 Direct Visual In Situ Hybridization (DIRVISH); 2.7.1 Preparation of Stretched DNA 2.7.2 FISH Hybridization2.7.3 Detection and Analysis; 2.7.4 Microscopy; 2.8 Troubleshooting; CHAPTER 3. Detection of Nucleic Acids (DNA and RNA) In Situ by Single and Cyclic Primed In Situ labelling (PRINS): Two Alternatives to Traditional In Situ Hybridization Methods; 3.1 Introduction; 3.2 Materials and Chemicals; 3.2.1 Buffers and Reagents; 3.2.2 Chemicals; 3.2.3 Equipment; 3.3 DNA-PRINS with Oligonucleotide Probes; 3.4 PRINS with Ddel Digested Cloned Probes; 3.4.1 Generation of Primers from Cloned Alpha Satellite DNA; 3.4.2 PRlNS Reaction; 3.5 Multicolour-PRINS; 3.6 PRINS-painting 3.7 PRINS-PCR and Repeated-PRINS of DNA3.7.1 Chromosome Spreads; 3.7.2 Pretreatment of Chromosome Spreads; 3.7.3 Labelling Reaction; 3.8 PRINS-PCR of mRNA; 3.9 Visualization of Hapten-Labelled Nucleotides; 3.9.1 Standard Slides; 3.9.1.1 Biotin; 3.9.1.2 Digoxigenin; 3.9.2 Micro-slides; 3.9.2.1 Detection of Labelled DNA; 3.9.2.2 Detection of Labelled RNA; 3.9.2.3 Alkaline Phosphatase Visualization; 3.9.2.4 Fluorescent Visualization; 3.10 Troubleshooting CHAPTER 4. Fluorescence lmmunophenotyping and lnterphase Cytogenetics as a Tool for Investigation of Neoplasms (FICTION): Combined In Situ Hybridization and Fluorescence lmmunophenotyping |
Record Nr. | UNINA-9910830987403321 |
[Saddle River, NJ?, : Wiley-VCH, 2002?] | ||
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Lo trovi qui: Univ. Federico II | ||
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Molecular biology of the gene / J. D. Watson ; with illustrations by Keith Richards |
Autore | Watson, James D. |
Edizione | [2. ed] |
Pubbl/distr/stampa | New York, : W. A. Benjamin, 1970 |
Descrizione fisica | XXI, 662 p. : ill. ; 23 cm |
Disciplina | 574.87322 |
Soggetto non controllato | Gene - Biologia molecolare |
ISBN |
805390200
805396039 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNINA-9910800401003321 |
Watson, James D.
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New York, : W. A. Benjamin, 1970 | ||
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Lo trovi qui: Univ. Federico II | ||
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The inconstant gene / Lawrence S. Dillon |
Autore | Dillon, Lawrence S. |
Pubbl/distr/stampa | New York, : Plenum Press, c1983 |
Descrizione fisica | viii, 581 p ; 24 cm |
Disciplina | 574.87322 |
Collana | The Evolution and functioning of the genetic mechanism |
ISBN | 0306410842 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNINA-9910870099503321 |
Dillon, Lawrence S.
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New York, : Plenum Press, c1983 | ||
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Lo trovi qui: Univ. Federico II | ||
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Transcriptomics and Gene Regulation / / edited by Jiaqian Wu |
Edizione | [1st ed. 2016.] |
Pubbl/distr/stampa | Dordrecht : , : Springer Netherlands : , : Imprint : Springer, , 2016 |
Descrizione fisica | 1 online resource (190 p.) |
Disciplina | 574.87322 |
Collana | Translational Bioinformatics |
Soggetto topico |
Bioinformatics
Gene expression Neurosciences Gene Expression |
ISBN | 94-017-7450-1 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | The Analyses of Global Gene Expression and Transcription Factor Regulation -- Global Approaches to Alternative Splicing and its Regulation - Recent Advances and Open Questions -- Long Non-coding RNAs: Critical Regulators for Cell Lineage Commitment in the Central Nervous System -- Gene expression models of signaling pathways -- From Gene Expression to Disease Phenotypes: Network-Based Approaches to Study Complex Human Diseases -- Introduction to Isoform Sequencing using Pacific Biosciences Technology (Iso-Seq) -- Systematic and Integrative Analysis of Gene Expression to Identify Feature Genes Underlying Human Diseases. |
Record Nr. | UNINA-9910253874403321 |
Dordrecht : , : Springer Netherlands : , : Imprint : Springer, , 2016 | ||
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Lo trovi qui: Univ. Federico II | ||
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