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Patch clamping [[electronic resource] ] : an introductory guide to patch clamp electrophysiology / / Areles Molleman
Patch clamping [[electronic resource] ] : an introductory guide to patch clamp electrophysiology / / Areles Molleman
Autore Molleman Areles
Pubbl/distr/stampa New York, : J. Wiley, c2003
Descrizione fisica 1 online resource (187 p.)
Disciplina 571.64
572/.437
Soggetto topico Patch-clamp techniques (Electrophysiology)
ISBN 1-280-27051-9
9786610270514
0-470-85651-3
0-470-85652-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Patch Clamping; Contents; Preface; 1 Introduction; 1.1 Patch Clamping and its Context; 2 Basic Theoretical Principles; 2.1 Introduction to Membrane Biology; 2.1.1 The plasma membrane and its ionic environment; 2.1.2 Electrochemical gradients and the Nernst equation; 2.1.3 Maintenance of ion gradients and the membrane potential; 2.1.4 Ion channels; 2.2 Electrical Properties of the Cell Membrane; 2.2.1 Driving force and membrane resistance; 2.2.2 Membrane capacitance; 2.2.3 Consequences of membrane capacitance; 2.2.4 An electronic model of the plasma membrane
2.3 Recording Modes and their Equivalent Circuits2.3.1 The basics of equivalent circuits; 2.3.2 Intracellular recording; 2.3.3 Voltage clamp and current clamp; 2.3.4 Introduction to patch clamp configurations; 2.3.5 The equivalent circuit for the cell-attached patch configuration; 2.3.6 The equivalent circuit for the whole-cell configuration; 2.3.7 The equivalent circuit for the excised patch configurations; 3 Requirements; 3.1 The Platform; 3.1.1 Stability: vibrations and drift; 3.1.2 Where in the building should the set-up be placed?; 3.1.3 Anti-vibration tables; 3.2 Mechanics and Optics
3.2.1 The microscope3.2.2 Micromanipulators; 3.2.3 Pipette pressure; 3.2.4 Baths and superfusion systems; 3.3 Electrodes and Micropipettes; 3.3.1 Solid-liquid junction potentials and polarisation; 3.3.2 The bath electrode; 3.3.3 Micropipettes; 3.3.4 Liquid junction potentials; 3.4 Electronics; 3.4.1 External noise and Faraday cages; 3.4.2 Patch clamp amplifiers; 3.4.3 Noise prevention and signal conditioning; 3.4.4 Data acquisition and digitisation; 3.4.5 Computers and software; 4 The Practice of Patch Clamping; 4.1 Preparing the Experiment and Making a Seal; 4.1.1 Setting up
4.1.2 Bringing the pipette near the preparation4.1.3 Making the seal; 4.2 Whole-cell Modes; 4.2.1 Conventional whole-cell recording; 4.2.2 Perforated patch recording; 4.3 Single-channel Modes; 4.3.1 General notes; 4.3.2 Cell-attached patch; 4.3.3 Excised patches; 5 Whole-cell Protocols and Data Analysis; 5.1 Standard Cellular Parameters; 5.2 Voltage-activated Currents; 5.2.1 Introduction to pulse protocols; 5.2.2 Signal conditioning and positive/negative subtraction; 5.2.3 Space clamp artefacts; 5.2.4 Isolation of a homogeneous population of channels
5.2.5 Current-voltage relationships and reversal potential5.2.6 Determination of relative permeabilities; 5.2.7 Activation and inactivation studies; 5.3 Non-voltage-activated Currents; 5.3.1 Introduction to continuous recording; 5.3.2 Determination of reversal potential using voltage ramps; 6 Single-channel Protocols and Data Analysis; 6.1 General Single-channel Practice and Analysis; 6.1.1 Practical notes; 6.1.2 Amplitude analysis; 6.1.3 Event detection; 6.1.4 Dwell time analysis; 6.2 Continuous Recording of Single Channels; 6.2.1 Data acquisition; 6.2.2 Spontaneous activity
6.2.3 Receptor-induced activity
Record Nr. UNINA-9910830071403321
Molleman Areles  
New York, : J. Wiley, c2003
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Patch clamping [[electronic resource] ] : an introductory guide to patch clamp electrophysiology / / Areles Molleman
Patch clamping [[electronic resource] ] : an introductory guide to patch clamp electrophysiology / / Areles Molleman
Autore Molleman Areles
Pubbl/distr/stampa New York, : J. Wiley, c2003
Descrizione fisica 1 online resource (187 p.)
Disciplina 571.64
572/.437
Soggetto topico Patch-clamp techniques (Electrophysiology)
ISBN 1-280-27051-9
9786610270514
0-470-85651-3
0-470-85652-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Patch Clamping; Contents; Preface; 1 Introduction; 1.1 Patch Clamping and its Context; 2 Basic Theoretical Principles; 2.1 Introduction to Membrane Biology; 2.1.1 The plasma membrane and its ionic environment; 2.1.2 Electrochemical gradients and the Nernst equation; 2.1.3 Maintenance of ion gradients and the membrane potential; 2.1.4 Ion channels; 2.2 Electrical Properties of the Cell Membrane; 2.2.1 Driving force and membrane resistance; 2.2.2 Membrane capacitance; 2.2.3 Consequences of membrane capacitance; 2.2.4 An electronic model of the plasma membrane
2.3 Recording Modes and their Equivalent Circuits2.3.1 The basics of equivalent circuits; 2.3.2 Intracellular recording; 2.3.3 Voltage clamp and current clamp; 2.3.4 Introduction to patch clamp configurations; 2.3.5 The equivalent circuit for the cell-attached patch configuration; 2.3.6 The equivalent circuit for the whole-cell configuration; 2.3.7 The equivalent circuit for the excised patch configurations; 3 Requirements; 3.1 The Platform; 3.1.1 Stability: vibrations and drift; 3.1.2 Where in the building should the set-up be placed?; 3.1.3 Anti-vibration tables; 3.2 Mechanics and Optics
3.2.1 The microscope3.2.2 Micromanipulators; 3.2.3 Pipette pressure; 3.2.4 Baths and superfusion systems; 3.3 Electrodes and Micropipettes; 3.3.1 Solid-liquid junction potentials and polarisation; 3.3.2 The bath electrode; 3.3.3 Micropipettes; 3.3.4 Liquid junction potentials; 3.4 Electronics; 3.4.1 External noise and Faraday cages; 3.4.2 Patch clamp amplifiers; 3.4.3 Noise prevention and signal conditioning; 3.4.4 Data acquisition and digitisation; 3.4.5 Computers and software; 4 The Practice of Patch Clamping; 4.1 Preparing the Experiment and Making a Seal; 4.1.1 Setting up
4.1.2 Bringing the pipette near the preparation4.1.3 Making the seal; 4.2 Whole-cell Modes; 4.2.1 Conventional whole-cell recording; 4.2.2 Perforated patch recording; 4.3 Single-channel Modes; 4.3.1 General notes; 4.3.2 Cell-attached patch; 4.3.3 Excised patches; 5 Whole-cell Protocols and Data Analysis; 5.1 Standard Cellular Parameters; 5.2 Voltage-activated Currents; 5.2.1 Introduction to pulse protocols; 5.2.2 Signal conditioning and positive/negative subtraction; 5.2.3 Space clamp artefacts; 5.2.4 Isolation of a homogeneous population of channels
5.2.5 Current-voltage relationships and reversal potential5.2.6 Determination of relative permeabilities; 5.2.7 Activation and inactivation studies; 5.3 Non-voltage-activated Currents; 5.3.1 Introduction to continuous recording; 5.3.2 Determination of reversal potential using voltage ramps; 6 Single-channel Protocols and Data Analysis; 6.1 General Single-channel Practice and Analysis; 6.1.1 Practical notes; 6.1.2 Amplitude analysis; 6.1.3 Event detection; 6.1.4 Dwell time analysis; 6.2 Continuous Recording of Single Channels; 6.2.1 Data acquisition; 6.2.2 Spontaneous activity
6.2.3 Receptor-induced activity
Record Nr. UNINA-9910840572103321
Molleman Areles  
New York, : J. Wiley, c2003
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Pharmacogenomics of human drug transporters [[electronic resource] ] : clinical impacts / / edited by Toshihisa Ishikawa, Richard B. Kim, Jörg König
Pharmacogenomics of human drug transporters [[electronic resource] ] : clinical impacts / / edited by Toshihisa Ishikawa, Richard B. Kim, Jörg König
Pubbl/distr/stampa Hoboken, N.J., : Wiley, c2013
Descrizione fisica 1 online resource (441 p.)
Disciplina 571.64
Altri autori (Persone) IshikawaToshihisa
KimRichard B
KönigJörg
Soggetto topico Biological transport
Drug Interactions
Pharmacogenetics
ISBN 1-118-35324-2
1-118-35325-0
1-299-38528-1
1-118-35320-X
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Introduction to pharmacogenomics of drug transporters / Marianne K DeGorter and Richard B Kim -- ADME pharmacogenomics in drug development / Liangfu Chen and Joseph W. Polli -- Regulatory perspective on pharmacogenomics of drug metabolizing enzymes and transporters / Lei Zhang ... [et al.] -- The pharmacogenomics of membrane transporters project / Sook Wah Yee, Deanna L. Kroetz, Kathleen M. Giacomini -- Emerging new technology of SNP typing / Toshihisa Ishikawa and Yoshihide Hayashizaki -- Chapters 6: OATP1A2, OAT1 and OAT3 / Rommel G. Tirona -- OATP1B1, OATB1B3 and OATP2B1 / Jorg Konig and Martin Fromm -- OCT (SLC22A) and OCTN families / Sophie L. Stocker ... [et al.] -- MATE (SLC47) family / Atsushi Yonezawa and Ken-ichi Inui -- PEPT (SLC15A) family / Tomoko Sugiura ... [et al.] -- Nucleoside transporters (SLC28 and SLC29) family / Míriam Molina-Arcas -- Maral pastor-anglada -- P-glycoprotein (MDR1/ABCB1) / Ingolf Cascorbi -- Bile salt export pump BSEP (ABCB11) : role in liver physiology and liver disease / Gerd Ullak-Kublick and Bruno Stieger -- BCRP (ABCG2) / Agnes Basseville ... [et al.] -- MRP2 (ABCC2) and MRP3 (ABCC3) / Anne T. Nies -- MRP4 (ABCC4) / Satish B.Cheepala, Mugdha Sukthankar, John D. Schuetz -- MRP8 (ABCC11) / Yu Toyoda, Toshihisa Ishikawa -- Future perspectives / Toshihisa Ishikawa, Joseph Ware.
Record Nr. UNINA-9910139050103321
Hoboken, N.J., : Wiley, c2013
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Pharmacogenomics of human drug transporters [[electronic resource] ] : clinical impacts / / edited by Toshihisa Ishikawa, Richard B. Kim, Jörg König
Pharmacogenomics of human drug transporters [[electronic resource] ] : clinical impacts / / edited by Toshihisa Ishikawa, Richard B. Kim, Jörg König
Edizione [1st ed.]
Pubbl/distr/stampa Hoboken, N.J., : Wiley, c2013
Descrizione fisica 1 online resource (441 p.)
Disciplina 571.64
Altri autori (Persone) IshikawaToshihisa
KimRichard B
KönigJörg
Soggetto topico Biological transport
Drug Interactions
Pharmacogenetics
ISBN 1-118-35324-2
1-118-35325-0
1-299-38528-1
1-118-35320-X
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Introduction to pharmacogenomics of drug transporters / Marianne K DeGorter and Richard B Kim -- ADME pharmacogenomics in drug development / Liangfu Chen and Joseph W. Polli -- Regulatory perspective on pharmacogenomics of drug metabolizing enzymes and transporters / Lei Zhang ... [et al.] -- The pharmacogenomics of membrane transporters project / Sook Wah Yee, Deanna L. Kroetz, Kathleen M. Giacomini -- Emerging new technology of SNP typing / Toshihisa Ishikawa and Yoshihide Hayashizaki -- Chapters 6: OATP1A2, OAT1 and OAT3 / Rommel G. Tirona -- OATP1B1, OATB1B3 and OATP2B1 / Jorg Konig and Martin Fromm -- OCT (SLC22A) and OCTN families / Sophie L. Stocker ... [et al.] -- MATE (SLC47) family / Atsushi Yonezawa and Ken-ichi Inui -- PEPT (SLC15A) family / Tomoko Sugiura ... [et al.] -- Nucleoside transporters (SLC28 and SLC29) family / Míriam Molina-Arcas -- Maral pastor-anglada -- P-glycoprotein (MDR1/ABCB1) / Ingolf Cascorbi -- Bile salt export pump BSEP (ABCB11) : role in liver physiology and liver disease / Gerd Ullak-Kublick and Bruno Stieger -- BCRP (ABCG2) / Agnes Basseville ... [et al.] -- MRP2 (ABCC2) and MRP3 (ABCC3) / Anne T. Nies -- MRP4 (ABCC4) / Satish B.Cheepala, Mugdha Sukthankar, John D. Schuetz -- MRP8 (ABCC11) / Yu Toyoda, Toshihisa Ishikawa -- Future perspectives / Toshihisa Ishikawa, Joseph Ware.
Record Nr. UNINA-9910816186703321
Hoboken, N.J., : Wiley, c2013
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Phospholipases in Health and Disease [[electronic resource] /] / edited by Paramjit S. Tappia, Naranjan S. Dhalla
Phospholipases in Health and Disease [[electronic resource] /] / edited by Paramjit S. Tappia, Naranjan S. Dhalla
Edizione [1st ed. 2014.]
Pubbl/distr/stampa New York, NY : , : Springer New York : , : Imprint : Springer, , 2014
Descrizione fisica 1 online resource (416 p.)
Disciplina 571.64
Collana Advances in Biochemistry in Health and Disease
Soggetto topico Lipids
Molecular biology
Enzymology
Proteins 
Neurosciences
Cell membranes 
Lipidology
Molecular Medicine
Receptors
Membrane Biology
ISBN 1-4939-0464-7
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Phospholipases in Health and Disease -- Role of Phospholipases in Regulation of Cardiolipin Biosynthesis and Remodelling in the Heart and Mammalian Cells -- Role of Phospholipases and Oxidized Phospholipids in Inflammation -- Phospholipases in Cardiovascular Disease -- The Structures and Functions of Intracellular Phospholipase A1 Family Proteins -- Phospholipase A and Breast Cancer -- Pathophysiological Aspects of Lipoprotein Associated Phospholipase A2: A Brief Overview -- Phospholipase A2 Activity Exhibited by a Bacterial Virulence Protein that Enters and Operates within a Variety of Host Cells -- Expression and Role of Phospholipase A2 in Central Nervous System Injury and Disease -- Cytoplasmic Phospholipase A2 and Autotaxin Inhibitors as Potential Radiosensitizers -- Phospholipase A2 Enzymes: Potential Targets for Therapy -- The Role of Phospholipase C Isozymes in Cellular Homeostasis -- Phospholipase C in the Immune Cell -- Phosphoinositide-specific Phospholipase C Enzymes and Cognitive Development  and Decline -- Where life begins: Sperm PLCz in mammalian egg activation and implications in male infertility -- Oocyte Activation and Phospholipase C zeta (PLCζ): Male Infertility and  Implications for Therapeutic Intervention -- Phospholipase C Signaling in Heart Disease -- Activation of Phospholipase C in Cardiac Hypertrophy -- The Protective Effect of Phospholipase C From Cardiac Ischemia-Reperfusion Injury -- Role of Phospholipase C in Cardioprotection During Oxidative Stress -- Mammalian Phospholipase D: Structure, Regulation and Physiological Functions of Phospholipase D and its Link to Pathology -- Emerging Roles of Phospholipase D in Pathophysiological Signaling -- Alterations in Phospholipase D During the Development of Myocardial Disease.
Record Nr. UNINA-9910298337603321
New York, NY : , : Springer New York : , : Imprint : Springer, , 2014
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Physicochemical kinetics and transport at biointerfaces [[electronic resource] /] / edited by Herman P. van Leeuwen, Wolfgang Köster
Physicochemical kinetics and transport at biointerfaces [[electronic resource] /] / edited by Herman P. van Leeuwen, Wolfgang Köster
Pubbl/distr/stampa Hoboken, NJ, : John Wiley, 2004
Descrizione fisica 1 online resource (573 p.)
Disciplina 571.6/4
571.64
Altri autori (Persone) LeeuwenH. P. van
KösterWolfgang
Collana IUPAC series on analytical and physical chemistry of environmental systems
Soggetto topico Biological interfaces
Chemical kinetics
Biological transport
Soggetto genere / forma Electronic books.
ISBN 1-280-26953-7
9786610269532
0-470-09403-6
0-470-09404-4
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Physicochemical Kinetics and Transport at Biointerfaces; Contents; List of Contributors; Series Preface; Preface; 1 Physicochemical Kinetics and Transport at the Biointerface: Setting the Stage; 2 Molecular Modelling of Biological Membranes: Structure and Permeation Properties; 3 Biointerfaces and Mass Transfer; 4 Dynamics of Biouptake Processes: the Role of Transport, Adsorption and Internalisation; 5 Chemical Speciation of Organics and of Metals at Biological Interphases; 6 Transport of Solutes Across Biological Membranes: Prokaryotes
7 Transport of Solutes Across Biological Membranes in Eukaryotes: an Environmental Perspective8 Transport of Colloids and Particles Across Biological Membranes; 9 Mobilisation of Organic Compounds and Iron by Microorganisms; 10 Critical Evaluation of the Physicochemical Parameters and Processes for Modelling the Biological Uptake of Trace Metals in Environmental (Aquatic) Systems; Index; Colour Plates
Record Nr. UNINA-9910143642603321
Hoboken, NJ, : John Wiley, 2004
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Physicochemical kinetics and transport at biointerfaces [[electronic resource] /] / edited by Herman P. van Leeuwen, Wolfgang Köster
Physicochemical kinetics and transport at biointerfaces [[electronic resource] /] / edited by Herman P. van Leeuwen, Wolfgang Köster
Pubbl/distr/stampa Hoboken, NJ, : John Wiley, 2004
Descrizione fisica 1 online resource (573 p.)
Disciplina 571.6/4
571.64
Altri autori (Persone) LeeuwenH. P. van
KösterWolfgang
Collana IUPAC series on analytical and physical chemistry of environmental systems
Soggetto topico Biological interfaces
Chemical kinetics
Biological transport
ISBN 1-280-26953-7
9786610269532
0-470-09403-6
0-470-09404-4
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Physicochemical Kinetics and Transport at Biointerfaces; Contents; List of Contributors; Series Preface; Preface; 1 Physicochemical Kinetics and Transport at the Biointerface: Setting the Stage; 2 Molecular Modelling of Biological Membranes: Structure and Permeation Properties; 3 Biointerfaces and Mass Transfer; 4 Dynamics of Biouptake Processes: the Role of Transport, Adsorption and Internalisation; 5 Chemical Speciation of Organics and of Metals at Biological Interphases; 6 Transport of Solutes Across Biological Membranes: Prokaryotes
7 Transport of Solutes Across Biological Membranes in Eukaryotes: an Environmental Perspective8 Transport of Colloids and Particles Across Biological Membranes; 9 Mobilisation of Organic Compounds and Iron by Microorganisms; 10 Critical Evaluation of the Physicochemical Parameters and Processes for Modelling the Biological Uptake of Trace Metals in Environmental (Aquatic) Systems; Index; Colour Plates
Record Nr. UNINA-9910830010103321
Hoboken, NJ, : John Wiley, 2004
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Physicochemical kinetics and transport at biointerfaces [[electronic resource] /] / edited by Herman P. van Leeuwen, Wolfgang Köster
Physicochemical kinetics and transport at biointerfaces [[electronic resource] /] / edited by Herman P. van Leeuwen, Wolfgang Köster
Pubbl/distr/stampa Hoboken, NJ, : John Wiley, 2004
Descrizione fisica 1 online resource (573 p.)
Disciplina 571.6/4
571.64
Altri autori (Persone) LeeuwenH. P. van
KösterWolfgang
Collana IUPAC series on analytical and physical chemistry of environmental systems
Soggetto topico Biological interfaces
Chemical kinetics
Biological transport
ISBN 1-280-26953-7
9786610269532
0-470-09403-6
0-470-09404-4
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Physicochemical Kinetics and Transport at Biointerfaces; Contents; List of Contributors; Series Preface; Preface; 1 Physicochemical Kinetics and Transport at the Biointerface: Setting the Stage; 2 Molecular Modelling of Biological Membranes: Structure and Permeation Properties; 3 Biointerfaces and Mass Transfer; 4 Dynamics of Biouptake Processes: the Role of Transport, Adsorption and Internalisation; 5 Chemical Speciation of Organics and of Metals at Biological Interphases; 6 Transport of Solutes Across Biological Membranes: Prokaryotes
7 Transport of Solutes Across Biological Membranes in Eukaryotes: an Environmental Perspective8 Transport of Colloids and Particles Across Biological Membranes; 9 Mobilisation of Organic Compounds and Iron by Microorganisms; 10 Critical Evaluation of the Physicochemical Parameters and Processes for Modelling the Biological Uptake of Trace Metals in Environmental (Aquatic) Systems; Index; Colour Plates
Record Nr. UNINA-9910841790803321
Hoboken, NJ, : John Wiley, 2004
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Physiology of membrane fluidity / editor Meir Shinitzky
Physiology of membrane fluidity / editor Meir Shinitzky
Pubbl/distr/stampa Boca Raton, Fla. : CRC Press, c1984
Descrizione fisica 2 v. : ill. ; 27 cm
Disciplina 571.64
Altri autori (Persone) Shinitzky, Meir
Soggetto topico Membranes (Biology)
Membranes (Biology) - Fluidity
Membranes (Biology) - Mechanical properties
ISBN 0849361419 (v.1)
0849361427 (v.2)
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNISALENTO-991003539759707536
Boca Raton, Fla. : CRC Press, c1984
Materiale a stampa
Lo trovi qui: Univ. del Salento
Opac: Controlla la disponibilità qui
Plasma-Catalysis for Environmental and Energy-Related Applications / / edited by Monica Magureanu, Corina Bradu
Plasma-Catalysis for Environmental and Energy-Related Applications / / edited by Monica Magureanu, Corina Bradu
Pubbl/distr/stampa Basel, Switzerland : , : MDPI - Multidisciplinary Digital Publishing Institute, , 2022
Descrizione fisica 1 online resource (94 pages)
Disciplina 571.64
Soggetto topico Plasma cell diseases
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910674027703321
Basel, Switzerland : , : MDPI - Multidisciplinary Digital Publishing Institute, , 2022
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui

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