Patch clamping [[electronic resource] ] : an introductory guide to patch clamp electrophysiology / / Areles Molleman |
Autore | Molleman Areles |
Pubbl/distr/stampa | New York, : J. Wiley, c2003 |
Descrizione fisica | 1 online resource (187 p.) |
Disciplina |
571.64
572/.437 |
Soggetto topico | Patch-clamp techniques (Electrophysiology) |
ISBN |
1-280-27051-9
9786610270514 0-470-85651-3 0-470-85652-1 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Patch Clamping; Contents; Preface; 1 Introduction; 1.1 Patch Clamping and its Context; 2 Basic Theoretical Principles; 2.1 Introduction to Membrane Biology; 2.1.1 The plasma membrane and its ionic environment; 2.1.2 Electrochemical gradients and the Nernst equation; 2.1.3 Maintenance of ion gradients and the membrane potential; 2.1.4 Ion channels; 2.2 Electrical Properties of the Cell Membrane; 2.2.1 Driving force and membrane resistance; 2.2.2 Membrane capacitance; 2.2.3 Consequences of membrane capacitance; 2.2.4 An electronic model of the plasma membrane
2.3 Recording Modes and their Equivalent Circuits2.3.1 The basics of equivalent circuits; 2.3.2 Intracellular recording; 2.3.3 Voltage clamp and current clamp; 2.3.4 Introduction to patch clamp configurations; 2.3.5 The equivalent circuit for the cell-attached patch configuration; 2.3.6 The equivalent circuit for the whole-cell configuration; 2.3.7 The equivalent circuit for the excised patch configurations; 3 Requirements; 3.1 The Platform; 3.1.1 Stability: vibrations and drift; 3.1.2 Where in the building should the set-up be placed?; 3.1.3 Anti-vibration tables; 3.2 Mechanics and Optics 3.2.1 The microscope3.2.2 Micromanipulators; 3.2.3 Pipette pressure; 3.2.4 Baths and superfusion systems; 3.3 Electrodes and Micropipettes; 3.3.1 Solid-liquid junction potentials and polarisation; 3.3.2 The bath electrode; 3.3.3 Micropipettes; 3.3.4 Liquid junction potentials; 3.4 Electronics; 3.4.1 External noise and Faraday cages; 3.4.2 Patch clamp amplifiers; 3.4.3 Noise prevention and signal conditioning; 3.4.4 Data acquisition and digitisation; 3.4.5 Computers and software; 4 The Practice of Patch Clamping; 4.1 Preparing the Experiment and Making a Seal; 4.1.1 Setting up 4.1.2 Bringing the pipette near the preparation4.1.3 Making the seal; 4.2 Whole-cell Modes; 4.2.1 Conventional whole-cell recording; 4.2.2 Perforated patch recording; 4.3 Single-channel Modes; 4.3.1 General notes; 4.3.2 Cell-attached patch; 4.3.3 Excised patches; 5 Whole-cell Protocols and Data Analysis; 5.1 Standard Cellular Parameters; 5.2 Voltage-activated Currents; 5.2.1 Introduction to pulse protocols; 5.2.2 Signal conditioning and positive/negative subtraction; 5.2.3 Space clamp artefacts; 5.2.4 Isolation of a homogeneous population of channels 5.2.5 Current-voltage relationships and reversal potential5.2.6 Determination of relative permeabilities; 5.2.7 Activation and inactivation studies; 5.3 Non-voltage-activated Currents; 5.3.1 Introduction to continuous recording; 5.3.2 Determination of reversal potential using voltage ramps; 6 Single-channel Protocols and Data Analysis; 6.1 General Single-channel Practice and Analysis; 6.1.1 Practical notes; 6.1.2 Amplitude analysis; 6.1.3 Event detection; 6.1.4 Dwell time analysis; 6.2 Continuous Recording of Single Channels; 6.2.1 Data acquisition; 6.2.2 Spontaneous activity 6.2.3 Receptor-induced activity |
Record Nr. | UNINA-9910830071403321 |
Molleman Areles
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||
New York, : J. Wiley, c2003 | ||
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Lo trovi qui: Univ. Federico II | ||
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Patch clamping [[electronic resource] ] : an introductory guide to patch clamp electrophysiology / / Areles Molleman |
Autore | Molleman Areles |
Pubbl/distr/stampa | New York, : J. Wiley, c2003 |
Descrizione fisica | 1 online resource (187 p.) |
Disciplina |
571.64
572/.437 |
Soggetto topico | Patch-clamp techniques (Electrophysiology) |
ISBN |
1-280-27051-9
9786610270514 0-470-85651-3 0-470-85652-1 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Patch Clamping; Contents; Preface; 1 Introduction; 1.1 Patch Clamping and its Context; 2 Basic Theoretical Principles; 2.1 Introduction to Membrane Biology; 2.1.1 The plasma membrane and its ionic environment; 2.1.2 Electrochemical gradients and the Nernst equation; 2.1.3 Maintenance of ion gradients and the membrane potential; 2.1.4 Ion channels; 2.2 Electrical Properties of the Cell Membrane; 2.2.1 Driving force and membrane resistance; 2.2.2 Membrane capacitance; 2.2.3 Consequences of membrane capacitance; 2.2.4 An electronic model of the plasma membrane
2.3 Recording Modes and their Equivalent Circuits2.3.1 The basics of equivalent circuits; 2.3.2 Intracellular recording; 2.3.3 Voltage clamp and current clamp; 2.3.4 Introduction to patch clamp configurations; 2.3.5 The equivalent circuit for the cell-attached patch configuration; 2.3.6 The equivalent circuit for the whole-cell configuration; 2.3.7 The equivalent circuit for the excised patch configurations; 3 Requirements; 3.1 The Platform; 3.1.1 Stability: vibrations and drift; 3.1.2 Where in the building should the set-up be placed?; 3.1.3 Anti-vibration tables; 3.2 Mechanics and Optics 3.2.1 The microscope3.2.2 Micromanipulators; 3.2.3 Pipette pressure; 3.2.4 Baths and superfusion systems; 3.3 Electrodes and Micropipettes; 3.3.1 Solid-liquid junction potentials and polarisation; 3.3.2 The bath electrode; 3.3.3 Micropipettes; 3.3.4 Liquid junction potentials; 3.4 Electronics; 3.4.1 External noise and Faraday cages; 3.4.2 Patch clamp amplifiers; 3.4.3 Noise prevention and signal conditioning; 3.4.4 Data acquisition and digitisation; 3.4.5 Computers and software; 4 The Practice of Patch Clamping; 4.1 Preparing the Experiment and Making a Seal; 4.1.1 Setting up 4.1.2 Bringing the pipette near the preparation4.1.3 Making the seal; 4.2 Whole-cell Modes; 4.2.1 Conventional whole-cell recording; 4.2.2 Perforated patch recording; 4.3 Single-channel Modes; 4.3.1 General notes; 4.3.2 Cell-attached patch; 4.3.3 Excised patches; 5 Whole-cell Protocols and Data Analysis; 5.1 Standard Cellular Parameters; 5.2 Voltage-activated Currents; 5.2.1 Introduction to pulse protocols; 5.2.2 Signal conditioning and positive/negative subtraction; 5.2.3 Space clamp artefacts; 5.2.4 Isolation of a homogeneous population of channels 5.2.5 Current-voltage relationships and reversal potential5.2.6 Determination of relative permeabilities; 5.2.7 Activation and inactivation studies; 5.3 Non-voltage-activated Currents; 5.3.1 Introduction to continuous recording; 5.3.2 Determination of reversal potential using voltage ramps; 6 Single-channel Protocols and Data Analysis; 6.1 General Single-channel Practice and Analysis; 6.1.1 Practical notes; 6.1.2 Amplitude analysis; 6.1.3 Event detection; 6.1.4 Dwell time analysis; 6.2 Continuous Recording of Single Channels; 6.2.1 Data acquisition; 6.2.2 Spontaneous activity 6.2.3 Receptor-induced activity |
Record Nr. | UNINA-9910840572103321 |
Molleman Areles
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||
New York, : J. Wiley, c2003 | ||
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Lo trovi qui: Univ. Federico II | ||
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Pharmacogenomics of human drug transporters [[electronic resource] ] : clinical impacts / / edited by Toshihisa Ishikawa, Richard B. Kim, Jörg König |
Pubbl/distr/stampa | Hoboken, N.J., : Wiley, c2013 |
Descrizione fisica | 1 online resource (441 p.) |
Disciplina | 571.64 |
Altri autori (Persone) |
IshikawaToshihisa
KimRichard B KönigJörg |
Soggetto topico |
Biological transport
Drug Interactions Pharmacogenetics |
ISBN |
1-118-35324-2
1-118-35325-0 1-299-38528-1 1-118-35320-X |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | Introduction to pharmacogenomics of drug transporters / Marianne K DeGorter and Richard B Kim -- ADME pharmacogenomics in drug development / Liangfu Chen and Joseph W. Polli -- Regulatory perspective on pharmacogenomics of drug metabolizing enzymes and transporters / Lei Zhang ... [et al.] -- The pharmacogenomics of membrane transporters project / Sook Wah Yee, Deanna L. Kroetz, Kathleen M. Giacomini -- Emerging new technology of SNP typing / Toshihisa Ishikawa and Yoshihide Hayashizaki -- Chapters 6: OATP1A2, OAT1 and OAT3 / Rommel G. Tirona -- OATP1B1, OATB1B3 and OATP2B1 / Jorg Konig and Martin Fromm -- OCT (SLC22A) and OCTN families / Sophie L. Stocker ... [et al.] -- MATE (SLC47) family / Atsushi Yonezawa and Ken-ichi Inui -- PEPT (SLC15A) family / Tomoko Sugiura ... [et al.] -- Nucleoside transporters (SLC28 and SLC29) family / Míriam Molina-Arcas -- Maral pastor-anglada -- P-glycoprotein (MDR1/ABCB1) / Ingolf Cascorbi -- Bile salt export pump BSEP (ABCB11) : role in liver physiology and liver disease / Gerd Ullak-Kublick and Bruno Stieger -- BCRP (ABCG2) / Agnes Basseville ... [et al.] -- MRP2 (ABCC2) and MRP3 (ABCC3) / Anne T. Nies -- MRP4 (ABCC4) / Satish B.Cheepala, Mugdha Sukthankar, John D. Schuetz -- MRP8 (ABCC11) / Yu Toyoda, Toshihisa Ishikawa -- Future perspectives / Toshihisa Ishikawa, Joseph Ware. |
Record Nr. | UNINA-9910139050103321 |
Hoboken, N.J., : Wiley, c2013 | ||
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Lo trovi qui: Univ. Federico II | ||
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Pharmacogenomics of human drug transporters [[electronic resource] ] : clinical impacts / / edited by Toshihisa Ishikawa, Richard B. Kim, Jörg König |
Edizione | [1st ed.] |
Pubbl/distr/stampa | Hoboken, N.J., : Wiley, c2013 |
Descrizione fisica | 1 online resource (441 p.) |
Disciplina | 571.64 |
Altri autori (Persone) |
IshikawaToshihisa
KimRichard B KönigJörg |
Soggetto topico |
Biological transport
Drug Interactions Pharmacogenetics |
ISBN |
1-118-35324-2
1-118-35325-0 1-299-38528-1 1-118-35320-X |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | Introduction to pharmacogenomics of drug transporters / Marianne K DeGorter and Richard B Kim -- ADME pharmacogenomics in drug development / Liangfu Chen and Joseph W. Polli -- Regulatory perspective on pharmacogenomics of drug metabolizing enzymes and transporters / Lei Zhang ... [et al.] -- The pharmacogenomics of membrane transporters project / Sook Wah Yee, Deanna L. Kroetz, Kathleen M. Giacomini -- Emerging new technology of SNP typing / Toshihisa Ishikawa and Yoshihide Hayashizaki -- Chapters 6: OATP1A2, OAT1 and OAT3 / Rommel G. Tirona -- OATP1B1, OATB1B3 and OATP2B1 / Jorg Konig and Martin Fromm -- OCT (SLC22A) and OCTN families / Sophie L. Stocker ... [et al.] -- MATE (SLC47) family / Atsushi Yonezawa and Ken-ichi Inui -- PEPT (SLC15A) family / Tomoko Sugiura ... [et al.] -- Nucleoside transporters (SLC28 and SLC29) family / Míriam Molina-Arcas -- Maral pastor-anglada -- P-glycoprotein (MDR1/ABCB1) / Ingolf Cascorbi -- Bile salt export pump BSEP (ABCB11) : role in liver physiology and liver disease / Gerd Ullak-Kublick and Bruno Stieger -- BCRP (ABCG2) / Agnes Basseville ... [et al.] -- MRP2 (ABCC2) and MRP3 (ABCC3) / Anne T. Nies -- MRP4 (ABCC4) / Satish B.Cheepala, Mugdha Sukthankar, John D. Schuetz -- MRP8 (ABCC11) / Yu Toyoda, Toshihisa Ishikawa -- Future perspectives / Toshihisa Ishikawa, Joseph Ware. |
Record Nr. | UNINA-9910816186703321 |
Hoboken, N.J., : Wiley, c2013 | ||
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Lo trovi qui: Univ. Federico II | ||
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Phospholipases in Health and Disease [[electronic resource] /] / edited by Paramjit S. Tappia, Naranjan S. Dhalla |
Edizione | [1st ed. 2014.] |
Pubbl/distr/stampa | New York, NY : , : Springer New York : , : Imprint : Springer, , 2014 |
Descrizione fisica | 1 online resource (416 p.) |
Disciplina | 571.64 |
Collana | Advances in Biochemistry in Health and Disease |
Soggetto topico |
Lipids
Molecular biology Enzymology Proteins Neurosciences Cell membranes Lipidology Molecular Medicine Receptors Membrane Biology |
ISBN | 1-4939-0464-7 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | Phospholipases in Health and Disease -- Role of Phospholipases in Regulation of Cardiolipin Biosynthesis and Remodelling in the Heart and Mammalian Cells -- Role of Phospholipases and Oxidized Phospholipids in Inflammation -- Phospholipases in Cardiovascular Disease -- The Structures and Functions of Intracellular Phospholipase A1 Family Proteins -- Phospholipase A and Breast Cancer -- Pathophysiological Aspects of Lipoprotein Associated Phospholipase A2: A Brief Overview -- Phospholipase A2 Activity Exhibited by a Bacterial Virulence Protein that Enters and Operates within a Variety of Host Cells -- Expression and Role of Phospholipase A2 in Central Nervous System Injury and Disease -- Cytoplasmic Phospholipase A2 and Autotaxin Inhibitors as Potential Radiosensitizers -- Phospholipase A2 Enzymes: Potential Targets for Therapy -- The Role of Phospholipase C Isozymes in Cellular Homeostasis -- Phospholipase C in the Immune Cell -- Phosphoinositide-specific Phospholipase C Enzymes and Cognitive Development and Decline -- Where life begins: Sperm PLCz in mammalian egg activation and implications in male infertility -- Oocyte Activation and Phospholipase C zeta (PLCζ): Male Infertility and Implications for Therapeutic Intervention -- Phospholipase C Signaling in Heart Disease -- Activation of Phospholipase C in Cardiac Hypertrophy -- The Protective Effect of Phospholipase C From Cardiac Ischemia-Reperfusion Injury -- Role of Phospholipase C in Cardioprotection During Oxidative Stress -- Mammalian Phospholipase D: Structure, Regulation and Physiological Functions of Phospholipase D and its Link to Pathology -- Emerging Roles of Phospholipase D in Pathophysiological Signaling -- Alterations in Phospholipase D During the Development of Myocardial Disease. |
Record Nr. | UNINA-9910298337603321 |
New York, NY : , : Springer New York : , : Imprint : Springer, , 2014 | ||
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Lo trovi qui: Univ. Federico II | ||
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Physicochemical kinetics and transport at biointerfaces [[electronic resource] /] / edited by Herman P. van Leeuwen, Wolfgang Köster |
Pubbl/distr/stampa | Hoboken, NJ, : John Wiley, 2004 |
Descrizione fisica | 1 online resource (573 p.) |
Disciplina |
571.6/4
571.64 |
Altri autori (Persone) |
LeeuwenH. P. van
KösterWolfgang |
Collana | IUPAC series on analytical and physical chemistry of environmental systems |
Soggetto topico |
Biological interfaces
Chemical kinetics Biological transport |
Soggetto genere / forma | Electronic books. |
ISBN |
1-280-26953-7
9786610269532 0-470-09403-6 0-470-09404-4 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Physicochemical Kinetics and Transport at Biointerfaces; Contents; List of Contributors; Series Preface; Preface; 1 Physicochemical Kinetics and Transport at the Biointerface: Setting the Stage; 2 Molecular Modelling of Biological Membranes: Structure and Permeation Properties; 3 Biointerfaces and Mass Transfer; 4 Dynamics of Biouptake Processes: the Role of Transport, Adsorption and Internalisation; 5 Chemical Speciation of Organics and of Metals at Biological Interphases; 6 Transport of Solutes Across Biological Membranes: Prokaryotes
7 Transport of Solutes Across Biological Membranes in Eukaryotes: an Environmental Perspective8 Transport of Colloids and Particles Across Biological Membranes; 9 Mobilisation of Organic Compounds and Iron by Microorganisms; 10 Critical Evaluation of the Physicochemical Parameters and Processes for Modelling the Biological Uptake of Trace Metals in Environmental (Aquatic) Systems; Index; Colour Plates |
Record Nr. | UNINA-9910143642603321 |
Hoboken, NJ, : John Wiley, 2004 | ||
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Lo trovi qui: Univ. Federico II | ||
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Physicochemical kinetics and transport at biointerfaces [[electronic resource] /] / edited by Herman P. van Leeuwen, Wolfgang Köster |
Pubbl/distr/stampa | Hoboken, NJ, : John Wiley, 2004 |
Descrizione fisica | 1 online resource (573 p.) |
Disciplina |
571.6/4
571.64 |
Altri autori (Persone) |
LeeuwenH. P. van
KösterWolfgang |
Collana | IUPAC series on analytical and physical chemistry of environmental systems |
Soggetto topico |
Biological interfaces
Chemical kinetics Biological transport |
ISBN |
1-280-26953-7
9786610269532 0-470-09403-6 0-470-09404-4 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Physicochemical Kinetics and Transport at Biointerfaces; Contents; List of Contributors; Series Preface; Preface; 1 Physicochemical Kinetics and Transport at the Biointerface: Setting the Stage; 2 Molecular Modelling of Biological Membranes: Structure and Permeation Properties; 3 Biointerfaces and Mass Transfer; 4 Dynamics of Biouptake Processes: the Role of Transport, Adsorption and Internalisation; 5 Chemical Speciation of Organics and of Metals at Biological Interphases; 6 Transport of Solutes Across Biological Membranes: Prokaryotes
7 Transport of Solutes Across Biological Membranes in Eukaryotes: an Environmental Perspective8 Transport of Colloids and Particles Across Biological Membranes; 9 Mobilisation of Organic Compounds and Iron by Microorganisms; 10 Critical Evaluation of the Physicochemical Parameters and Processes for Modelling the Biological Uptake of Trace Metals in Environmental (Aquatic) Systems; Index; Colour Plates |
Record Nr. | UNINA-9910830010103321 |
Hoboken, NJ, : John Wiley, 2004 | ||
![]() | ||
Lo trovi qui: Univ. Federico II | ||
|
Physicochemical kinetics and transport at biointerfaces [[electronic resource] /] / edited by Herman P. van Leeuwen, Wolfgang Köster |
Pubbl/distr/stampa | Hoboken, NJ, : John Wiley, 2004 |
Descrizione fisica | 1 online resource (573 p.) |
Disciplina |
571.6/4
571.64 |
Altri autori (Persone) |
LeeuwenH. P. van
KösterWolfgang |
Collana | IUPAC series on analytical and physical chemistry of environmental systems |
Soggetto topico |
Biological interfaces
Chemical kinetics Biological transport |
ISBN |
1-280-26953-7
9786610269532 0-470-09403-6 0-470-09404-4 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Physicochemical Kinetics and Transport at Biointerfaces; Contents; List of Contributors; Series Preface; Preface; 1 Physicochemical Kinetics and Transport at the Biointerface: Setting the Stage; 2 Molecular Modelling of Biological Membranes: Structure and Permeation Properties; 3 Biointerfaces and Mass Transfer; 4 Dynamics of Biouptake Processes: the Role of Transport, Adsorption and Internalisation; 5 Chemical Speciation of Organics and of Metals at Biological Interphases; 6 Transport of Solutes Across Biological Membranes: Prokaryotes
7 Transport of Solutes Across Biological Membranes in Eukaryotes: an Environmental Perspective8 Transport of Colloids and Particles Across Biological Membranes; 9 Mobilisation of Organic Compounds and Iron by Microorganisms; 10 Critical Evaluation of the Physicochemical Parameters and Processes for Modelling the Biological Uptake of Trace Metals in Environmental (Aquatic) Systems; Index; Colour Plates |
Record Nr. | UNINA-9910841790803321 |
Hoboken, NJ, : John Wiley, 2004 | ||
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Lo trovi qui: Univ. Federico II | ||
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Physiology of membrane fluidity / editor Meir Shinitzky |
Pubbl/distr/stampa | Boca Raton, Fla. : CRC Press, c1984 |
Descrizione fisica | 2 v. : ill. ; 27 cm |
Disciplina | 571.64 |
Altri autori (Persone) | Shinitzky, Meir |
Soggetto topico |
Membranes (Biology)
Membranes (Biology) - Fluidity Membranes (Biology) - Mechanical properties |
ISBN |
0849361419 (v.1)
0849361427 (v.2) |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNISALENTO-991003539759707536 |
Boca Raton, Fla. : CRC Press, c1984 | ||
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Lo trovi qui: Univ. del Salento | ||
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Plasma-Catalysis for Environmental and Energy-Related Applications / / edited by Monica Magureanu, Corina Bradu |
Pubbl/distr/stampa | Basel, Switzerland : , : MDPI - Multidisciplinary Digital Publishing Institute, , 2022 |
Descrizione fisica | 1 online resource (94 pages) |
Disciplina | 571.64 |
Soggetto topico | Plasma cell diseases |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNINA-9910674027703321 |
Basel, Switzerland : , : MDPI - Multidisciplinary Digital Publishing Institute, , 2022 | ||
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Lo trovi qui: Univ. Federico II | ||
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